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Nanosuspension-Based Aloe vera Gel of Silver Sulfadiazine with Improved Wound Healing Activity

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Abstract

The present study focuses on the development and characterization of nanosuspension of a poorly soluble drug, silver sulfadiazine (SSD) incorporated in Aloe vera gel (AV-gel) for improving its therapeutic efficacy. The SSD solution in ammonia was subjected to nanoprecipitation in surfactant solution and particle size was optimized by varying concentration of surfactant. Optimized formulation constituted of 5.5% (w/v) Span 20 and 5.5% (w/v) Tween 80 as a dispersing agent and 0.5% (w/v) Poloxamer 188 as a co-surfactant. The prepared nanosuspension was evaluated for particle size, polydispersity index, surface morphology, and x-ray diffraction study. The optimized nanosuspension was incorporated into nanogel formulation with the addition of 1% AV-gel and 0.5% Carbopol 940 for topical delivery of nanosized SSD. Evaluation of in vitro drug release exhibited a significant enhancement in release rate of the drug from developed nanogel formulation (77.16 ± 3.241%) in comparison to marketed formulation (42.81 ± 1.452%) after 48 h. In vivo histopathological studies in rats for 14 days of application of prepared nanogel showed improvement in the wound healing potential as compared to marketed formulation.

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Acknowledgements

The authors are thankful to Anju Drug chem, Indore, India, for gift samples of silver sulfadiazine. The first author acknowledges the University Grant Commission and Ministry of Social Justice, Government of India for providing Research fellowship.

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Authors and Affiliations

  1. Nanomedicine Research Laboratory, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India

    Md Abul Barkat, Iqbal Ahmad & Farhan Jalees Ahmad

  2. Department of Pharmaceutics, Faculty of Pharmacy, Integral University, Kursi Road, Lucknow, India

    Md Abul Barkat & Satya Prakash Singh

  3. Department of Pharmaceutics, College of Pharmacy, SGIT, Ghaziabad, UP, India

    Harshita

  4. College of Pharmacy, King Saud University, P.O. Box No. 2457, Riyadh, 11451, Kingdom of Saudi Arabia

    Raisuddin Ali

  5. PG Department of Pharmaceutical Sciences, Faculty of Applied Sciences and Technology, University of Kashmir, Srinagar, J & K, India

    Faheem Hyder Pottoo

  6. Jubilant Generics Limited, Noida, UP, India

    Sarwar Beg

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  1. Md Abul Barkat
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  2. Harshita
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  3. Iqbal Ahmad
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  8. Farhan Jalees Ahmad
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Correspondence to Farhan Jalees Ahmad.

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Barkat, M.A., Harshita, Ahmad, I. et al. Nanosuspension-Based Aloe vera Gel of Silver Sulfadiazine with Improved Wound Healing Activity. AAPS PharmSciTech 18, 3274–3285 (2017). https://doi.org/10.1208/s12249-017-0817-y

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  • DOI: https://doi.org/10.1208/s12249-017-0817-y

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