Topical Formulation Containing Beeswax-Based Nanoparticles Improved In Vivo Skin Barrier Function

Abstract

Lipid nanoparticles have shown many advantages for treatment/prevention of skin disorders with damaged skin barrier function. Beeswax is a favorable candidate for the development of nanosystems in the cosmetic and dermatological fields because of its advantages for the development of products for topical application. In the present study, beeswax-based nanoparticles (BNs) were prepared using the hot melt microemulsion technique and incorporated to a gel-cream formulation. The formulation was subsequently evaluated for its rheological stability and effect on stratum corneum water content (SCWC) and transepidermal water loss (TEWL) using in vivo biophysical techniques. BNs resulted in mean particle size of 95.72 ± 9.63 nm and zeta potential of −9.85 ± 0.57 mV. BN-loaded formulation showed shear thinning behavior, well adjusted by the Herschel-Bulkley model, and a small thixotropy index that were stable for 28 days at different temperatures. BN-loaded formulation was also able to simultaneously decrease the TEWL and increase the SCWC values 28 days after treatment. In conclusion, the novel beeswax-based nanoparticles showed potential for barrier recovery and open the perspective for its commercial use as a novel natural active as yet unexplored in the field of dermatology and cosmetics for treatment of skin diseases with damaged skin barrier function.

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Acknowledgments

The authors would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) and the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Brazil) for the financial support to this study.

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Correspondence to Patrícia Maria Berardo Gonçalves Maia Campos.

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Souza, C., de Freitas, L.A.P. & Maia Campos, P.M.B.G. Topical Formulation Containing Beeswax-Based Nanoparticles Improved In Vivo Skin Barrier Function. AAPS PharmSciTech 18, 2505–2516 (2017). https://doi.org/10.1208/s12249-017-0737-x

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KEY WORDS

  • beeswax
  • clinical efficacy
  • lipid nanoparticles
  • rheology
  • skin barrier function