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Gentamicin-Eluting Titanium Dioxide Nanotubes Grown on the Ultrafine-Grained Titanium

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ABSTRACT

Titanium (Ti)-based materials is the most appropriate choices for the applications as orthopedic and dental implants. In this regard, ultrafine-grained (UFG) titanium with an enhanced mechanical properties and surface energy has attracted more attention. Titanium dioxide (TiO2) nanotubes grown on the titanium could enhance bone bonding, cellular response and are good reservoirs for loading drugs and antibacterial agents. This article investigates gentamicin loading into and release from the TiO2 nanotubes, grown on the UFG compared to coarse-grained (CG) titanium substrate surfaces. Equal Channel Angular Pressing (ECAP) was employed to produce the UFG structure titanium. TiO2 nanotubes were grown by the anodizing technique on both UFG and CG titanium substrate surfaces. Scanning electron microscopy (SEM) imaging confirmed TiO2 nanotube growth on the surface. The UV-vis spectroscopy analysis results show that the amount of gentamicin load-release in the anodized UFG titanium sample is higher than that of CG one which can be explained in terms of thicker TiO2 nanotube arrays layer formed on UFG sample. Moreover, the anodized UFG titanium samples released the drug in a longer time than CG (1 day for the UFG titanium vs. 3 h for the CG one). Regarding wettability analysis, anodized UFG titanium sample showed more enhanced hydrophilicity than CG counterpart. Therefore, the significantly smaller grain size of pure titanium provided by the ECAP technique coupled with appropriate subsequent anodization treatment not only offers a good combination of biocompatibility and adequate mechanical properties but also it provides a delayed release condition for gentamicin.

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ACKNOWLEDGEMENTS

Authors greatly acknowledge Dr. Saeed Hajizadeh (Maxillofacial surgeon and professor at the Shahid Beheshti University) for his valuable comments in conducting this research. The authors also would like to acknowledge the financial support of Iran National Science Foundation (INSF) and Iran Nanotechnology Initiative Council (INIC) for conducting this study.

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Authors and Affiliations

  1. Biomedical Engineering Faculty, Amirkabir University of Technology, Tehran, Iran

    Sima Hashemi Nemati & Afra Hadjizadeh

  2. Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran

    Afra Hadjizadeh

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  1. Sima Hashemi Nemati
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  2. Afra Hadjizadeh
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Correspondence to Afra Hadjizadeh.

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Nemati, S.H., Hadjizadeh, A. Gentamicin-Eluting Titanium Dioxide Nanotubes Grown on the Ultrafine-Grained Titanium. AAPS PharmSciTech 18, 2180–2187 (2017). https://doi.org/10.1208/s12249-016-0679-8

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  • DOI: https://doi.org/10.1208/s12249-016-0679-8

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