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Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice

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Abstract

The physicochemical properties of the optimized microemulsion and the permeating ability of oxyresveratrol in microemulsion were evaluated, and the efficacy of oxyresveratrol microemulsion in cutaneous herpes simplex virus type 1 (HSV-1) infection in mice was examined. The optimized microemulsion was composed of 10% w/w of isopropyl myristate, 35% w/w of Tween 80, 35% w/w of isopropyl alcohol, and 20% w/w of water. The mean particle diameter was 9.67 ± 0.58 nm, and the solubility of oxyresveratrol in the microemulsion was 196.34 ± 0.80 mg/ml. After accelerated and long-term stability testing, the microemulsion base and oxyresveratrol-loaded microemulsion were stable. The cumulative amount of oxyresveratrol permeating through shed snake skin from microemulsion at 6 h was 93.04 times compared to that of oxyresveratrol from Vaseline, determined at 20% w/w concentration. In cutaneous HSV-1 infection in mice, oxyresveratrol microemulsion at 20%, 25%, and 30% w/w, topically applied five times daily for 7 days after infection, was significantly effective in delaying the development of skin lesions and protecting from death (p < 0.05) compared with the untreated control. Oxyresveratrol microemulsion at 25% and 30% w/w was significantly more effective than that of 30% w/w of oxyresveratrol in Vaseline (p < 0.05) and was as effective as 5% w/w of acyclovir cream, topically applied five times daily (p > 0.05). These results demonstrated that topical oxyresveratrol microemulsion at 20–30% w/w was suitable for cutaneous HSV-1 mouse infection.

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ACKNOWLEDGMENTS

We gratefully thank the Japan Society for the Promotion of Science 2009, University of Toyama, Chulalongkorn University Centenary Academic Development Project (2008–2009) and Chulalongkorn University Graduate Scholarship Commemorating the 72nd Anniversary of His Majesty King (2008–2010) for the support of this work. We thank Tomoko Okuda for her excellent assistance.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok, 10330, Thailand

    Pattaraporn Sasivimolphan, Vimolmas Lipipun & Khanidtha Chitphet

  2. Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Garnpimol Ritthidej

  3. Department of Virology, University of Toyama, 2630 Sugitani, Toyama, 939-0194, Japan

    Yoshihiro Yoshida, Tohru Daikoku & Kimiyasu Shiraki

  4. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Boonchoo Sritularak & Kittisak Likhitwitayawuid

  5. Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Pornpen Pramyothin

  6. Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 939-0194, Japan

    Masao Hattori

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  1. Pattaraporn Sasivimolphan
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Sasivimolphan, P., Lipipun, V., Ritthidej, G. et al. Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice. AAPS PharmSciTech 13, 1266–1275 (2012). https://doi.org/10.1208/s12249-012-9828-x

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