Abstract
Brain tumor treatment employing methotrexate (MTX) is limited by the efflux mechanism of Pg-p on the blood–brain barrier. We aimed to investigate MTX-loaded chitosan or glycol chitosan (GCS) nanoparticles (NPs) in the presence and in the absence of a coating layer of Tween 80 for brain delivery of MTX. The effect of a low Tween 80 concentration was evaluated. MTX NPs were formulated following the ionic gelation technique and size and zeta potential measurements were acquired. Transport across MDCKII-MDR1 monolayer and cytotoxicity studies against C6 glioma cell line were also performed. Cell/particles interaction was visualized by confocal microscopy. The particles were shown to be cytotoxic against C6 cells line and able to overcome MDCKII-MDR1 cell barrier. GCS-based NPs were the most cytotoxic NPs. Confocal observations highlighted the internalization of Tween 80-coated fluorescent NPs more than Tween 80-uncoated NPs. The results suggest that even a low concentration of Tween 80 is sufficient for enhancing the transport of MTX from the NPs across MDCKII-MDR1 cells. The nanocarriers represent a promising strategy for the administration of MTX to brain tumors which merits further investigations under in vivo conditions.
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ACKNOWLEDGMENTS
This project was financed by Università degli Studi di Bari (Progetti di Ateneo 2008). We thank Dr. Antonella Loverre (Dipartimento di Emergenza Trapianti di Organi, (D.E.T.O.) Bari Hospital, Bari, Italy) for interpretation of confocal microphotographs. We also thank Prof. Giuseppe Trapani (University of Bari) for helpful discussions.
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Trapani, A., Denora, N., Iacobellis, G. et al. Methotrexate-Loaded Chitosan- and Glycolchitosan-Based Nanoparticles: A Promising Strategy for the Administration of the Anticancer Drug to Brain Tumors. AAPS PharmSciTech 12, 1302–1311 (2011). https://doi.org/10.1208/s12249-011-9695-x
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DOI: https://doi.org/10.1208/s12249-011-9695-x