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Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

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Abstract

The aim of the study was to develop ultrathin polyelectrolyte microreservoir (UPM) using two combinations of synthetic/synthetic (S/s; poly(allylamine hydrochloride) (PAH)/sodium poly(styrenesulfonate)) and synthetic/natural (S/n; PAH/sodium alginate) polyelectrolytes over spherical porous CaCO3 core particles (CP) followed by core removal and to evaluate its biocompatibility and integrity of loaded model protein bovine serum albumin (BSA). A novel process for synthesis of CP was developed to obtain maximum yield of monodisperse vaterite (spherical) polymorph. The prepared UPM was characterized for surface morphology, layer-by-layer growth, pay load efficiency, integrity of BSA, as well as viability and cell adhesion using murine J 774 macrophages (Φ). In vitro release profile revealed that both S/s and S/n UPM were able to provide sufficient diffusion barrier to release protein at physiological pH. It has been observed that S/n UPM are fully biocompatible due to obvious reason of using natural polymer. In a separate experiment, the S/s UPM surface was modified with pluronic F-68 to tune biocompatibility which provides evidences for safety and tolerability of the S/s UPM as well. In nutshell, the proposed system could successfully be used for the delivery of proteins, and moreover, the system can be tailored to impart desired properties at any stage of layering especially in terms of drug release and to retain the integrity of proteins.

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Acknowledgments

Financial assistance to Dr. P.R. Mishra through CSIR XI Network project (NWP 0035) on “Nanomaterials and Nanodevices for applications in Health and diseases” is gratefully acknowledged. Girish K Gupta and V. Jain are thankful to Indian Council of Medical Research and Council of Scientific and Industrial Research, New-Delhi, India for providing Senior Research Fellowships and Research Associate fellowship, respectively. Prof. S.C. Lakhotia, Department of Zoology, Banaras Hindu University, Varanasi, India is gratefully acknowledged for providing Confocal Laser Scanning Microphotography facility. The CDRI communication no. is 7409.

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  1. Pharmaceutics Division, Central Drug Research Institute, CSIR, Chattar Manzil Palace, M.G. Marg, Lucknow, 226001, India

    Girish K. Gupta, Vikas Jain & Prabhat Ranjan Mishra

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  1. Girish K. Gupta
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  2. Vikas Jain
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Correspondence to Prabhat Ranjan Mishra.

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Gupta, G.K., Jain, V. & Mishra, P.R. Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation. AAPS PharmSciTech 12, 344–353 (2011). https://doi.org/10.1208/s12249-011-9593-2

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