Abstract
The multikinase inhibitor sorafenib (SOR) is used to treat patients with hepatocellular and renal carcinomas. SOR undergoes CYP-mediated biotransformation to a pharmacologically active N-oxide metabolite (SNO) that has been shown to accumulate to varying extents in individuals. Kinase inhibitors like SOR are frequently coadministered with a range of other drugs to improve the efficacy of anticancer drug therapy and to treat comorbidities. Recent evidence has suggested that SNO is more effective than SOR as an inhibitor of CYP3A4-mediated midazolam 1′-hydroxylation. CYP2D6 is also reportedly inhibited by SOR. The present study assessed the possibility that SNO might contribute to CYP2D6 inhibition. The inhibition kinetics of CYP2D6-mediated dextromethorphan O-demethylation were analyzed in human hepatic microsomes, with SNO found to be ~ 19-fold more active than SOR (Kis 1.8 ± 0.3 μM and 34 ± 11 μM, respectively). Molecular docking studies of SOR and SNO were undertaken using multiple crystal structures of CYP2D6. Both molecules mediated interactions with key amino acid residues in putative substrate recognition sites of CYP2D6. However, a larger number of H-bonding interactions was noted between the N-oxide moiety of SNO and active site residues that account for its greater inhibition potency. These findings suggest that SNO has the potential to contribute to pharmacokinetic interactions involving SOR, perhaps in those individuals in whom SNO accumulates.
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Abbreviations
- AUC:
-
area under the serum concentration versus time curve
- C max :
-
maximal serum concentration
- CYP:
-
cytochrome P450
- DDI:
-
drug-drug interaction
- SOR:
-
sorafenib
- SNO:
-
sorafenib N-oxide
- SRS:
-
substrate recognition site
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Acknowledgments
Technical contributions in aspects of the study from Dr. S. Cui and Ms. K Bourget are also acknowledged. The supply of the human liver samples used in this study by Dr. J George is also gratefully acknowledged.
Funding
This study is financially supported by the Cancer Council NSW (grants RG09-14 and IG11-33). PCN is supported by the Flinders Centre for Innovation in Cancer (FCIC) and Flinders Medical Centre (FMC) Foundation through an Early Career Research Grant.
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Murray, M., Gillani, T.B., Rawling, T. et al. Inhibition of Hepatic CYP2D6 by the Active N-Oxide Metabolite of Sorafenib. AAPS J 21, 107 (2019). https://doi.org/10.1208/s12248-019-0374-2
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DOI: https://doi.org/10.1208/s12248-019-0374-2