Skip to main content

Advertisement

SpringerLink
Log in

Hidalgo, I. J., Raub, T. J., and Borchardt, R. T.: Characterization of the Human Colon Carcinoma Cell Line (Caco-2) as a Model System for Intestinal Epithelial Permeability, Gastroenterology, 96, 736–749, 1989—The Backstory

  • Commentary
  • Theme: Landmark Publications in the Pharmaceutical Sciences: The Backstory
  • Published:
The AAPS Journal Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Audus KA, Borchardt RT. Characterization of an in vitro blood–brain barrier model system for studying drug transport and metabolism. Pharm Res. 1986;3:81–7.

    Article  CAS  Google Scholar 

  2. Osiecka I, Borchardt RT, Fix JA, Gardner CA, Porter PA. In vitro drug absorption models. I. Brush border membrane vesicles, isolated mucosal cells and everted intestinal rings: characterization and salicylate accumulation. Pharm Res. 1985;2:284–93.

    Article  Google Scholar 

  3. Porter PA, Osiecka I, Borchardt RT, Fix JA, Frost L, Gardner CA. In vitro drug absorption models. II. Salicylate, cefoxitin, α-methyldopa and theophylline uptake in cells and rings: correlation with in vivo bioavailability. Pharm Res. 1985;2:293–8.

    Article  Google Scholar 

  4. Osiecka I, Cortese M, Porter PA, Borchardt RT, Fix JA, Gardner CR. Intestinal absorption of α-methyldopa: in vitro mechanistic studies in rat small intestinal segments. J Pharmacol Exp Ther. 1987;242:443–9.

    PubMed  CAS  Google Scholar 

  5. Leibovitz A, Stinson JC, McCombs WB, McCoy CE, Mazur KC, Mabry ND. Classification of human colorectal adenocarcinoma cell line. Cancer Res. 1976;36:4562–9.

    PubMed  CAS  Google Scholar 

  6. Pinto M, Appay M-D, Simon-Assmann P, Chevalier G, Dracopoli N, Fogh J, et al. Enterocytic differentiation of cultured human colon cancer cells by replacement of glucose by galactose in the medium. Biol Cell. 1982;44:193–6.

    CAS  Google Scholar 

  7. Pinto M, Robine-Leon S, Appay M-D, Kedinger M, Triadou N, Dussaulx E, et al. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol Cell. 1983;47:323–30.

    Google Scholar 

  8. Hidalgo IJ, Raub TJ, Kuentzel SL, Borchardt RT. Characterization of the Caco-2 cell line as an intestinal epithelial model system. FASEB J. 1988;2:A734. Abst. #2539.

    Google Scholar 

  9. Hidalgo IJ, Kato A, Borchardt RT. Epidermal growth factor (EGF) binding in a human colon carcinoma cell-line (Caco-2). FASEB J. 1989;3:A899. Abst. #3906.

    Google Scholar 

  10. Hidalgo IJ, Borchardt RT, Raub TJ. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology. 1989;96:736–49.

    PubMed  CAS  Google Scholar 

  11. Hidalgo IJ, Borchardt RT. Transport of taurocholic acid in an intestinal epithelial model system (Caco-2 cell). Pharm Res. 1988;5:S-110. Abst. #949.

    Google Scholar 

  12. Hidalgo IJ, Borchardt RT. Amino acid transport in a novel model system of the intestinal epithelium (Caco-2 cell). Pharm Res. 1988;5:S-110. Abst. #PD950.

    Google Scholar 

  13. Hidalgo IJ, Hillgren KM, Grass GM, Borchardt RT. Characterization of the aqueous boundary layer in Caco-2 cells using a novel diffusion cell. Pharm Res. 1989;6:S-114. Abst. #PD950.

    Google Scholar 

  14. Hidalgo IJ, Borchardt RT. Polarity of bile acid uptake and efflux in an intestinal epithelial cell line. Pharm Res. 1989;6:S-115. Abst. #PD953.

    Google Scholar 

  15. Hidalgo IJ, Kato A, Borchardt RT. Epidermal growth factor (EGF) binding in a human colon carcinoma cell line (Caco-2). Biochem Biophys Res Commun. 1989;158:317–24.

    Article  Google Scholar 

  16. Hidalgo IJ, Borchardt RT. Transport of a large neutral amino acid (phenylalanine) in a human intestinal epithelial cell line: Caco-2. Biochim Biophys Acta. 1028;25–30:1990.

    Google Scholar 

  17. Hidalgo IJ, Borchardt RT. Transport of bile acids in a human intestinal epithelial cell line: Caco-2. Biochim Biophys Acta. 1035;97–103:1990.

    Google Scholar 

  18. Audus K, Bartel R, Hidalgo I, Borchardt RT. The use of cultured epithelial and endothelial cells for drug transport and metabolism studies. Pharm Res. 1990;7:435–51.

    Article  PubMed  CAS  Google Scholar 

  19. Hidalgo IJ, Borchardt RT. Carrier-mediated transport of bile acids and amino acids in Caco-2 cells. In: Wilson G, Davis SS, Illum L, editors. Pharmaceutical applications of cell and tissue culture to drug transport. New York: Plenum; 1991. p. 77–92.

    Google Scholar 

  20. Borchardt RT, Hidalgo IJ, Hillgren KM, Hu M. Pharmaceutical applications of cell culture—an overview. In: Wilson G, Davis SS, Illum L, editors. Pharmaceutical applications of cell and tissue culture to drug transport. New York: Plenum; 1991. p. 1–14.

    Google Scholar 

  21. Hidalgo IJ, Hillgren KM, Grass GM, Borchardt RT. Characterization of the unstirred water layer in Caco-2 cells using a novel diffusion cell. Pharm Res. 1991;8:223–7.

    Article  Google Scholar 

  22. Artursson P. Epithelial transport of drugs. I. A model for studying the transport of drugs (β-blocking agents) over an intestinal epithelial cell line (Caco-2). J Pharm Sci. 1990;79:476–82.

    Article  PubMed  CAS  Google Scholar 

  23. Artursson P, Magnusson C. Epithelial transport of drugs 2. Effect of extracellular calcium on the transport of hydrophilic and lipophilic drugs (ß-blocking agents) over an intestinal epithelial (Caco-2) cell line. J Pharm Sci. 1990;79:595–600.

    Article  PubMed  CAS  Google Scholar 

  24. Karlsson J, Artursson P. A method for the determination of cellular permeability coefficients and aqueous boundary layer thickness in monolayers of intestinal epithelial (Caco-2) cells grown in permeable filter chambers. Int J Pharm. 1991;71:55–64.

    Article  CAS  Google Scholar 

  25. Artursson P, Karlsson J. Correlation between oral drug absorption in humans and apparent drug permeability coefficients in human intestinal epithelial (Caco-2) cells. Biochem Biophys Res Commun. 1991;175:880–5.

    Article  PubMed  CAS  Google Scholar 

  26. Hillgers AR, Conradi RA, Burton PS. Caco-2 cell monolayers as a model for drug transport across the intestinal mucosa. Pharm Res. 1990;7:902–10.

    Article  Google Scholar 

  27. Hillgers AR, Conradi RA, Ho NF, Burton PS. The influence of peptide structure on transport across Caco-2 cells. Pharm Res. 1991;8:1453–60.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, Kansas, 66047, USA

    Ronald T. Borchardt

Authors
  1. Ronald T. Borchardt
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ronald T. Borchardt.

Additional information

Editor: Ho-Leung Fung

This manuscript was written with the knowledge and input of Ismael J. Hidalgo and Thomas J. Raub, who were coauthors of the 1989 Gastroenterology paper describing for the first time Caco-2 cell monolayers as a cell culture model of the intestinal mucosa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Borchardt, R.T. Hidalgo, I. J., Raub, T. J., and Borchardt, R. T.: Characterization of the Human Colon Carcinoma Cell Line (Caco-2) as a Model System for Intestinal Epithelial Permeability, Gastroenterology, 96, 736–749, 1989—The Backstory. AAPS J 13, 323–327 (2011). https://doi.org/10.1208/s12248-011-9283-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1208/s12248-011-9283-8

Key words

Search

Navigation