AAPS PharmSciTech

, 20:5 | Cite as

Brain Microdialysis Study of Vancomycin in the Cerebrospinal Fluid After Intracerebroventricular Administration in Mice

  • Yusuke Miura
  • Yuki Fuchigami
  • Sakiko Nomura
  • Koyo Nishimura
  • Masayori Hagimori
  • Shigeru KawakamiEmail author
Brief/Technical Note


Vancomycin (VCM) is an important antibiotic for treating methicillin-resistant Staphylococcus aureus (MRSA) infections. To treat bacterial meningitis caused by MRSA, it is necessary to deliver VCM into the meninges, but the rate of VCM translocation through the blood–brain barrier is poor. Additionally, high doses of intravascularly (i.v.) administered VCM may cause renal impairments. Thus, VCM is sometimes administered intracerebroventricularly (i.c.v.) for clinical treatment. However, information on the VCM pharmacokinetics in cerebrospinal fluid (CSF) after i.c.v. administration is lacking. In the present study, we evaluated the VCM pharmacokinetics in the CSF and systemic circulation after i.c.v. compared to that after i.v. administration, using the brain microdialysis method in mice. VCM administered via i.c.v. showed a highly selective distribution in the CSF, without migration to systemic circulation. Moreover, to assess renal impairments after i.c.v. administration of VCM, we histologically evaluated damage to the mouse kidney by hematoxylin and eosin staining. No significant morphological change in the kidney was observed in the i.c.v. administration group compared to that in the i.v. administration group. Our results demonstrate that i.c.v. administration of VCM can be partially prevented from entering the systemic circulation to prevent renal impairments caused by VCM.


intracerebroventricular administration vancomycin microdialysis pharmacokinetics 


Funding information

This work was partly supported by JSPS KAKENHI Grant Number 16K18862.


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Copyright information

© American Association of Pharmaceutical Scientists 2018

Authors and Affiliations

  • Yusuke Miura
    • 1
  • Yuki Fuchigami
    • 1
  • Sakiko Nomura
    • 1
  • Koyo Nishimura
    • 1
  • Masayori Hagimori
    • 1
  • Shigeru Kawakami
    • 1
    Email author
  1. 1.Department of Pharmaceutical Informatics, Graduate School of Biomedical SciencesNagasaki UniversityNagasakiJapan

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