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AAPS PharmSciTech

, Volume 19, Issue 7, pp 3258–3271 | Cite as

Thermal Magnetic Field Activated Propolis Release From Liquid Crystalline System Based on Magnetic Nanoparticles

  • Lucas de Alcântara Sica de Toledo
  • Hélen Cássia Rosseto
  • Rafaela Said dos Santos
  • Federico Spizzo
  • Lucia Del Bianco
  • Maiara Camotti Montanha
  • Elisabetta Esposito
  • Elza Kimura
  • Patricia de Souza Bonfim-Mendonça
  • Terezinha Inez Estivalet Svidzinski
  • Rita Cortesi
  • Marcos Luciano Bruschi
Research Article

Abstract

Intra-periodontal pocket drug delivery systems, such as liquid crystalline systems, are widely utilized improving the drug release control and the therapy. Propolis is used in the treatment of periodontal diseases, reducing the inflammatory and infectious conditions. Iron oxide magnetic nanoparticles (MNPs) can improve the treatment when an alternating external magnetic field (AEMF) is applied, increasing the local temperature. The aim of this study was to develop a liquid crystalline system containing MNPs for intra-periodontal pocket propolis release. MNPs were prepared using iron salts and the morphological, size, thermal, x-ray diffraction, magnetometry, and Mössbauer spectroscopy analyses were performed. Cytotoxicity studies using Artemia salina and fibroblasts were also accomplished. The systems were prepared using polyoxyethylene (10) oleyl ether, isopropyl myristate, purified water, and characterized by polarized optical microscopy, rheometry, and in vitro drug release profile using a periodontal pocket simulator apparatus. The antifungal activity of the systems was investigated against Candida spp. using an AEMF. MNPs displayed nanometric size, were monodisperse, and they displayed very low cytotoxicity. Microscopically homogeneous formulations were obtained displaying important physicochemical and biological properties. The system displayed prolonged release of propolis and important in vitro fungicide activity, which was increased when the AEMF was applied, indicating a potentially alternative therapy for the treatment of the periodontal disease.

KEY WORDS

magnetic nanoparticles drug delivery buccal natural products periodontal disease 

Notes

Acknowledgements

The authors thank the financial support of the Brazilian funding agencies CAPES (Coordination of Improvement of Higher Education Personnel), CNPq (National Counsel of Technological and Scientific Development; grant number 206085/2014-1), and FINEP (Financier of Studies and Projects).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12249_2018_1163_MOESM1_ESM.docx (4.1 mb)
ESM 1 (DOCX 4224 kb)

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Copyright information

© American Association of Pharmaceutical Scientists 2018

Authors and Affiliations

  • Lucas de Alcântara Sica de Toledo
    • 1
  • Hélen Cássia Rosseto
    • 1
  • Rafaela Said dos Santos
    • 1
  • Federico Spizzo
    • 2
  • Lucia Del Bianco
    • 2
  • Maiara Camotti Montanha
    • 3
  • Elisabetta Esposito
    • 4
  • Elza Kimura
    • 3
  • Patricia de Souza Bonfim-Mendonça
    • 5
  • Terezinha Inez Estivalet Svidzinski
    • 3
    • 5
  • Rita Cortesi
    • 4
  • Marcos Luciano Bruschi
    • 1
  1. 1.Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of PharmacyState University of MaringaMaringaBrazil
  2. 2.Department of Physics and Earth SciencesUniversity of FerraraFerraraItaly
  3. 3.Postgraduate Program in Biosciences and PhysiopathologyState University of MaringaMaringaBrazil
  4. 4.Department of Life Sciences and BiotechnologyUniversity of FerraraFerraraItaly
  5. 5.Laboratory of Medical Mycology, Department of Clinical Analysis and BiomedicineState University of MaringaMaringaBrazil

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