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AAPS PharmSciTech

, Volume 19, Issue 7, pp 3177–3186 | Cite as

In Vivo Determination of Moisturizers Efficacy on Human Skin Hydration by Confocal Raman Spectroscopy

  • Vamshi Krishna Tippavajhala
  • Taciana D. Magrini
  • Daniele C. Matsuo
  • Michely G. P. Silva
  • Priscila P. Favero
  • Leonardo Rodrigues De Paula
  • Airton Abrahão Martin
Research Article

Abstract

This research work deals with in vivo testing of the efficacy of commercial moisturizer products on the hydration of human skin, as there are various in vitro and ex vivo studies questioning their activity. Confocal Raman spectroscopy was used for this purpose of assessing the efficacy of moisturizers on skin hydration mainly owing to its simple, non-invasive, non-destructive, timesaving, and cost-effective nature. Water content and natural moisturizing factor (NMF) of stratum corneum were analyzed and compared using this method at high wavenumber (2500–4000 cm−1) and fingerprint (400–1800 cm−1) spectral regions, respectively, as these two parameters are correlated to skin hydration. Four commercial moisturizer products of different brands were tested on volar forearm region of healthy human female volunteers. This study was conducted for a period of 30 days with 0, 7, and 30 days as time points of analysis. The results of this study clearly indicate that not all the moisturizer products hydrate the skin to the expected levels, and this extent of skin hydration varies with duration of application of these products.

KEY WORDS

moisturizer efficacy in vivo human skin hydration confocal Raman spectroscopy 

Notes

Funding Information

Airton Abrahão Martin thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (310375/2017) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (project number 88881.068140/2014-01) for their financial support.

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Copyright information

© American Association of Pharmaceutical Scientists 2018

Authors and Affiliations

  1. 1.Department of Pharmaceutics, Manipal College of Pharmaceutical SciencesManipal Academy of Higher EducationManipalIndia
  2. 2.Laboratory of Biomedical Vibrational Spectroscopy (LEVB), Institute of Research and Development (IP&D)Universidade do Vale do Paraiba (UNIVAP)São José dos CamposBrazil
  3. 3.Biomedical Engineering Innovation Center, Biomedical Vibrational Spectroscopy GroupUniversidade Brasil-UNBrItaqueraBrazil
  4. 4.DermoProbes: Skin and Hair Technology OptimizationSão José dos CamposBrazil
  5. 5.Natura Inovação e Tecnologia de Produtos Ltda.—CajamarCajamarBrazil
  6. 6.Department of PhysicsFederal University of Piaui—UFPITeresinaBrazil

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