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AAPS PharmSciTech

, Volume 19, Issue 7, pp 3000–3008 | Cite as

Preparation and Characterization of Tetrahydrocurcumin-Loaded Cellulose Acetate Phthalate/Polyethylene Glycol Electrospun Nanofibers

  • Ravikumar Rramaswamy
  • Ganesh Mani
  • Senthil Venkatachalam
  • Ramesh Venkata Yasam
  • J. C. Bose Rajendran
  • Jang Hyun Tae
Research Article
  • 68 Downloads

Abstract

A simple composite electrospun nanofiber of cellulose acetate phthalate (CAP)-polyethylene glycol (PEG) loaded with tetrahydrocurcumin (THC) was developed in this study, and the in vitro diffusion of THC was evaluated. The nanofibers were characterized by scanning electron microscopy, powder X-ray diffraction (PXRD), Fourier-transform infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC). The formulated nanofiber (NF) with THC has smooth morphology with diameter of around 300–500 nm. The complete entrapment and dispersion of THC was observed from the results of PXRD and DSC due to the loss of THC crystalline property. Further, FT-IR demonstrated that the vibration bands for the polymers used were dominant over the THC, and the vibrational bands of THC were not observed from the final formulation. The drug entrapment by the final CAP + PEG NF was found to be 95.5% with the high swelling index. From the in vitro release study, it was found that the formulated THC-loaded CAP + PEG NF has followed anomalous mechanism, demonstrating both diffusion and swelling controlled modes. The drug release extended up to 12 h with a final cumulative release of 94.24%.

KEY WORDS

polymers tetrahydrocurcumin electrospinning nanofibers in vitro evaluations 

Notes

Acknowledgements

This work was supported by the Hanseo University Intramural Research Grant, South Korea (2017).

Compliance with Ethical Standards

Conflicts of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12249_2018_1122_MOESM1_ESM.doc (221 kb)
ESM 1 (DOC 221 kb)

References

  1. 1.
    Rashmi HP, Vandana BP, Medha DJ. Polymeric nanoparticles for targeted treatment in oncology: current insights. Int J Nanomedicine. 2015;10:1001–18.  https://doi.org/10.2147/ijn.S56932.CrossRefGoogle Scholar
  2. 2.
    Saravanakumar A, Ganesh M, Jun HJ, Je OC, Yun HC, Jung HL, et al. Preparation and characterization of gatifloxacin-loaded alginate/poly (vinyl alcohol) electrospun nanofibers. Artif Cells Nanomed Biotechnol. 2014;44:847–52.  https://doi.org/10.3109/21691401.2014.986676.CrossRefGoogle Scholar
  3. 3.
    Hu X, Liu S, Zhou G, Huang Y, Xie Z, Jing X. Electrospinning of polymeric nanofibers for drug delivery applications. J Control Release. 2014;185:12–21.  https://doi.org/10.1016/j.jconrel.2014.04.018.CrossRefPubMedGoogle Scholar
  4. 4.
    Wang B, Wang Y, Yin T, Yu Q. Applications of electrospinning technique in drug delivery. Chem Eng Commun. 2010;197:1315–38.  https://doi.org/10.1080/00986441003625997.CrossRefGoogle Scholar
  5. 5.
    Chakraborty S, Liao IC, Adler A, Leong KW. Electrohydrodynamics: a facile technique to fabricate drug delivery systems. Adv Drug Deliv Rev. 2009;61:1043–54.  https://doi.org/10.1016/j.addr.2009.07.013.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Mendoza PC, Ferrand A, Facca S, Fioretti F, Ladam G, Kuchler BS, et al. Smart hybrid materials equipped by nanoreservoirs of therapeutics. ACS Nano. 2012;6:483–90.  https://doi.org/10.1021/nn203817t.CrossRefGoogle Scholar
  7. 7.
    Ignatious F, Sun L, Lee CP, Baldoni J. Electrospun nanofibers in oral drug delivery. J Pharm Res. 2010;27:576–88.  https://doi.org/10.1007/s11095-010-0061-6. CrossRefGoogle Scholar
  8. 8.
    Yan S, Xiaoqiang L, Shuiping L, Xiumei M, Ramakrishna S. Controlled release of dual drugs from emulsion electrospun nanofibrous mats. Colloids Surf B Biointerfaces. 2009;73:376–81.  https://doi.org/10.1016/j.colsurfb.2009.06.009.CrossRefPubMedGoogle Scholar
  9. 9.
    Shen X, Yu D, Zhu L, Branford WC, White K, Chatterton NP. Electrospun diclofenac sodium loaded Eudragit® L 100-55 nanofibers for colon-targeted drug delivery. Int J Pharm. 2011;408:200–7.  https://doi.org/10.1016/j.ijpharm.2011.01.058.CrossRefPubMedGoogle Scholar
  10. 10.
    Yoo JJ, Kim C, Chung CW, Jeong YI, Kang D. 5-aminolevulinic acid-incorporated poly(vinyl alcohol) nanofiber-coated metal stent for application in photodynamic therapy. Int J Nanomedicine. 2012;7:1997–2005.  https://doi.org/10.2147/ijn.S30298.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Fathi AA, Qun L, Chan YW, Chan SY. Novel vitamin and gold-loaded nanofiber facial mask for topical delivery. AAPS PharmSciTech. 2010;11:1164–70.  https://doi.org/10.1208/s12249-010-9475-z.CrossRefGoogle Scholar
  12. 12.
    Opanasopit P, Ruktanonchai U, Suwantong O, Panomsuk S, Ngawhirunpat T, Sittisombut C, et al. Electrospun poly(vinyl alcohol) fiber mats as carriers for extracts from the fruit hull of mangosteen. J Cosmet Sci. 2008;59:233–42.PubMedGoogle Scholar
  13. 13.
    Chen DW, Liao JY, Liu SJ, Chan EC. Novel biodegradable sandwich structured nanofibrous drug-eluting membranes for repair of infected wounds: an in vitro and in vivo study. Int J Nanomedicine. 2012;7:763–71.  https://doi.org/10.2147/IJN.S29119.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Wu XM, Branford-White CJ, Zhu LM, Chatterton NP, Yu DG. Ester prodrug-loaded electrospun cellulose acetate fiber mats as transdermal drug delivery systems. J Mater Sci Mater Med. 2010;21:2403–11.  https://doi.org/10.1007/s10856-010-4100-y.CrossRefPubMedGoogle Scholar
  15. 15.
    Janairo RR, Henry JJ, Lee BL, Hashi CK, Derugin N, Lee R, et al. Heparin-modified small-diameter nanofibrous vascular grafts. IEEE Trans Nanobioscience. 2012;11:22–7.  https://doi.org/10.1109/tnb.2012.2188926.CrossRefPubMedGoogle Scholar
  16. 16.
    Prabaharan M, Jayakumar R, Nair SV. Electrospun nanofibrous scaffolds-current status and prospects in drug delivery. In: Jayakumar R, Nair S, editors. Biomedical applications of polymeric nanofibers, Advances in polymer science. Berlin: Springer; 2011. p. 241–62.CrossRefGoogle Scholar
  17. 17.
    Zhang Y, Lim CT, Ramakrishna S, Huang ZM. Recent development of polymer nanofibers for biomedical and biotechnological applications. J Mater Sci Mater Med. 2005;16:933–46.  https://doi.org/10.1007/s10856-005-4428-x.CrossRefPubMedGoogle Scholar
  18. 18.
    Sukhwinder KB, Harpal SB. Perspectives on nanofiber dressings for the localized delivery of botanical remedies in wound healing. AIMS Mater Sci. 2017;4:370–82.  https://doi.org/10.3934/matersci.2017.2.370.CrossRefGoogle Scholar
  19. 19.
    Abdelgawada AM, Hudsona SM, Rojasb OJ. Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems. Carbohydr Polym. 2014;100:166–78.  https://doi.org/10.1016/j.carbpol.2012.12.043.CrossRefGoogle Scholar
  20. 20.
    Kalaipriya M, Sridhar R, Sundarrajan S, Venugopal JR, Ramakrishna S. Vitamin B12 loaded poly caprolactone nanofibers: a novel transdermal route for the water soluble energy supplement delivery. Int J Pharm. 2013;444:70–6.CrossRefGoogle Scholar
  21. 21.
    Olaru N, Olaru L, Tudorachi N, Simona D, Manuela P. Nanostructures of cellulose acetate phthalate obtained by electrospinning from 2-methoxyethanol-containingsolvent systems: morphological aspects thermal behavior, and antimicrobial activity. Ind Eng Chem Res. 2013;52:696–705.  https://doi.org/10.1021/ie301299d.CrossRefGoogle Scholar
  22. 22.
    Zhang YZ, Feng Y, Huang ZM, Ramakrishna S, Lim CT. Fabrication of porous electrospun nanofibers. Nanotechnology. 2006;17:901–8.  https://doi.org/10.1088/0957-4484/17/3/047. CrossRefGoogle Scholar
  23. 23.
    Sugiyama Y, Kawakishi S, Osawa T. Involvement of the β-diketone moiety in the antioidative mechanism of tetrahydrocurcumin. Biochem Pharmacol. 1996;52:519–25.  https://doi.org/10.1016/0006-2952(96)00302-4.CrossRefPubMedGoogle Scholar
  24. 24.
    Mahendra Kumar T, Mayank G, Sambhu CM, Snehasis J. Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it’s implication for wound healing. J Food Sci Technol. 2017;54:1137–45.  https://doi.org/10.1007/s13197-017-2525-8.CrossRefGoogle Scholar
  25. 25.
    Saipin S, Wichan K, Duangkhae M, Ruedeekorn W. Controlled release of oral tetrahydrocurcumin from a novel self-emulsifying floating drug delivery system (SEFDDS). AAPS PharmSciTech. 2011;12:152–64.  https://doi.org/10.1208/s12249-010-9568-8.CrossRefGoogle Scholar
  26. 26.
    Wu JC, Tsai ML, Lai CS, Wang YJ, Ho CT, Pan MH. Chemopreventative effects of tetrahydrocurcumin on human diseases. Food Funct. 2014;5:12–27.  https://doi.org/10.1039/c3fo60370a.CrossRefPubMedGoogle Scholar
  27. 27.
    Shrestha R, Palat A, Punnoose AM, Joshi S, Ponraju D, Paul SFD. Electrospun cellulose acetate phthalate nanofibrous scaffolds fabricated using novel solvent combinations biocompatible for primary chondrocytes and neurons. Tissue Cell. 2016;18:634–43.  https://doi.org/10.1016/j.tice.2016.07.007.CrossRefGoogle Scholar
  28. 28.
    Xiaochen GU, Sreeneeranj K, Daryl JF, Frank JB. In-vitro permeation of the insect repellent N,N-diethyl-m-toluamide (DEET) and the sunscreen oxybenzone. J Pharm Pharmacol. 2004;56:621–8.  https://doi.org/10.1211/0022357023402.CrossRefGoogle Scholar
  29. 29.
    Prashant MS, Suniket VF, Avinash KD. Evaluation of polymerized rosin for the formulation and development of transdermal drug delivery system: a technical note. AAPS PharmSciTech. 2005;6:E649–54.  https://doi.org/10.1208/pt060481.CrossRefGoogle Scholar
  30. 30.
    Venkata RY, Satya LJ, Natarajan J, Senthil V, Gowthamarajan K, Sumeet S, et al. A novel vesicular transdermal delivery of nifedipine—preparation, characterization and in vitro/in-vivo evaluation. Drug Delivery. 2016;23:619–30.  https://doi.org/10.3109/10717544.2014.931484. CrossRefGoogle Scholar
  31. 31.
    Shiow-Fern Ng JJR, Francis DS, Victor M, Gillian ME. Validation of a static Franz diffusion cell system for in vitro permeation studies. AAPS PharmSciTech. 2010;11:1432–41.  https://doi.org/10.1208/s12249-010-9522-9.CrossRefPubMedPubMedCentralGoogle Scholar
  32. 32.
    Rolland A, Demichelis G, Jamoulle JC, Shroot B. Influence of formulation, receptor fluid, and occlusion, on in vitro drug release from topical dosage forms, using an automated flow-through diffusion cell. Pharm Res. 1992;9:82–6.  https://doi.org/10.1023/A:1018935912097. CrossRefPubMedGoogle Scholar
  33. 33.
    Olaru N, Olaru L. Electrospinning of cellulose acetate phthalate from different solvent systems. Ind Eng Chem Res. 2010;49:1953–7.  https://doi.org/10.1021/ie901427f.CrossRefGoogle Scholar
  34. 34.
    Ravikumar R, Ganesh M, Ubaidulla U, Choi EY, Jang HT. Preparation, characterization, and in vitro diffusion study of nonwoven electrospun nanofiber of curcumin-loaded cellulose acetate phthalate polymer. Saudi Pharm J. 2017;25:921–6.  https://doi.org/10.1016/j.jsps.2017.02.004.CrossRefPubMedPubMedCentralGoogle Scholar
  35. 35.
    Nezarati RM, Eifert MB, Cosgriff-Hernandez E. Effects of humidity and solution viscosity on electrospun Fiber morphology. Tissue Eng Part C Methods. 2013;19:810–9.  https://doi.org/10.1089/ten.tec.2012.0671.CrossRefPubMedPubMedCentralGoogle Scholar
  36. 36.
    Manjunath L, Sailaja RRN. PMMA—cellulose acetate phthalate nano composites reinforced with silane-treated nanoclay. Cellulose. 2014;21:1793–802.  https://doi.org/10.1007/s10570-014-0190-x.CrossRefGoogle Scholar
  37. 37.
    Roxin P, Karlsson A, Singh SK. Characterization of cellulose acetate phthalate (CAP). Drug Dev Ind Pharm. 1998;24:1025–41.  https://doi.org/10.3109/03639049809089946.CrossRefPubMedGoogle Scholar
  38. 38.
    Rana IR, Ming MW, Amal HEK. Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis. Int J Nanomedicine. 2017;12:2335–51.  https://doi.org/10.2147/IJN.S131253.CrossRefGoogle Scholar
  39. 39.
    Zhaoyang X, Jianyu L, Huan Z, Xiangdong J, Chuang Y, Fei W, et al. Morphological and swelling behavior of cellulose nanofiber (CNF)/poly(vinyl alcohol) (PVA) hydrogels: poly(ethylene glycol) (PEG) as porogen. RSC Adv. 2016;6:43626–33.  https://doi.org/10.1039/c6ra03620a.CrossRefGoogle Scholar
  40. 40.
    Hayashi M, Nagai T, Nogami H. Factors affecting dissolution rate of cellulose acetate phthalate in aqueous solution. Chem Pharm Bull. 1970;18:2350–3.CrossRefGoogle Scholar
  41. 41.
    Higuchi T. Rate of release of medicaments from ointment bases containing drugs in suspension. J Pharm Sci. 1961;50:874–5.CrossRefGoogle Scholar
  42. 42.
    Ritger PL, Peppas NA. A simple equation for description of solute release. II. Fickian and anomalous release from swellable devices. J Control Release. 1987;5:37–42.CrossRefGoogle Scholar

Copyright information

© American Association of Pharmaceutical Scientists 2018

Authors and Affiliations

  • Ravikumar Rramaswamy
    • 1
    • 2
  • Ganesh Mani
    • 2
  • Senthil Venkatachalam
    • 3
  • Ramesh Venkata Yasam
    • 3
  • J. C. Bose Rajendran
    • 4
  • Jang Hyun Tae
    • 2
  1. 1.Department of Advanced Materials Science and EngineeringHanseo UniversitySeosan-SiSouth Korea
  2. 2.Department of Chemical EngineeringHanseo UniversitySeosan-SiSouth Korea
  3. 3.Department of PharmaceuticsJSS College of PharmacyThe NilgirisIndia
  4. 4.Department of RadiologyStanford University School of MedicineStanfordUSA

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