Topical Formulation Containing Beeswax-Based Nanoparticles Improved In Vivo Skin Barrier Function
Lipid nanoparticles have shown many advantages for treatment/prevention of skin disorders with damaged skin barrier function. Beeswax is a favorable candidate for the development of nanosystems in the cosmetic and dermatological fields because of its advantages for the development of products for topical application. In the present study, beeswax-based nanoparticles (BNs) were prepared using the hot melt microemulsion technique and incorporated to a gel-cream formulation. The formulation was subsequently evaluated for its rheological stability and effect on stratum corneum water content (SCWC) and transepidermal water loss (TEWL) using in vivo biophysical techniques. BNs resulted in mean particle size of 95.72 ± 9.63 nm and zeta potential of −9.85 ± 0.57 mV. BN-loaded formulation showed shear thinning behavior, well adjusted by the Herschel-Bulkley model, and a small thixotropy index that were stable for 28 days at different temperatures. BN-loaded formulation was also able to simultaneously decrease the TEWL and increase the SCWC values 28 days after treatment. In conclusion, the novel beeswax-based nanoparticles showed potential for barrier recovery and open the perspective for its commercial use as a novel natural active as yet unexplored in the field of dermatology and cosmetics for treatment of skin diseases with damaged skin barrier function.
KEY WORDSbeeswax clinical efficacy lipid nanoparticles rheology skin barrier function
The authors would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) and the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Brazil) for the financial support to this study.
Compliance with ethical standards
Disclosure of Interest
The authors declare that they have no conflicts of interest.
- 12.Gowda DV, Gupta VK, Khan MS, Bathool A. Encapsulation of clozapine into beeswax microspheres: preparation, characterization and release kinetics. Int J PharmTech Res. 2011;3(4):2199–207.Google Scholar
- 22.Freitas LAP, Zamarioli CM, Martins RM. Brazilian Patent Office. INPI—Instituto Nacional da Propriedade Industrial. Processo de obtenção de nanopartículas lipídicas sólidas contendo curcuminóides, nanopartículas lipídicas sólidas contendo curcuminóides e uso das mesmas. Registered patent number: BR1020150090170, 22/04/2015, Brazil. 2015.Google Scholar
- 24.Zetasizer Nano-ZS. User Instructions. NBTC User Instructions. 2009.Google Scholar
- 30.Rigo LA, da Silva CR, de Oliveira SM, Cabreira TN, de Bona da Silva C, Ferreira J, et al. Nanoencapsulation of rice bran oil increases its protective effects against UVB radiation-induced skin injury in mice. Eur J Pharm Biopharm. 2015;93:11–7. doi: 10.1016/j.ejpb.2015.03.020.CrossRefPubMedGoogle Scholar
- 36.Barry BW. Rheology of dermatological vehicles. In: Dermatological Formulations—Percutaneous Absorption, Marcel Dekker, Inc., New York and Basel. 1983;18:351–396.Google Scholar
- 40.Silva AC, Amaral MH, González-Mira E, Santos D, Ferreira D. Solid lipid nanoparticles (SLN)-based hydrogels as potential carriers for oral transmucosal delivery of risperidone: Preparation and characterization studies. Colloids Surf B: Biointerfaces. 2012;93:241–8. doi: 10.1016/j.colsurfb.2012.01.014.CrossRefPubMedGoogle Scholar
- 43.Briceno MI. Rheology of suspensions and emulsions. In: Pharmaceutical Emulsions and Suspensions, Marcel Dekker, Inc., New York, 2000;557–607.Google Scholar
- 52.Camargo Júnior FB, Gaspar LR, Campos PMBGM. Skin moisturizing effect of panthenol-based formulations. J Cosmet Sci. 2011;62(4):361–70.Google Scholar