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AAPS PharmSciTech

, Volume 18, Issue 6, pp 1917–1924 | Cite as

Polymeric Nanofiber/Antifungal Formulations Using a Novel Co-extrusion Approach

  • Mohammad Mofidfar
  • Jia Wang
  • Lisa Long
  • Christopher L. Hager
  • Chairut Vareechon
  • Eric Pearlman
  • Eric Baer
  • Mahmoud GhannoumEmail author
  • Gary E. WnekEmail author
Research Article

ABSTRACT

We report the successful implementation of a novel melt co-extrusion process to fabricate ca. 1 μm diameter fibers of poly(caprolactone) (PCL) containing the antifungal compound clotrimazole in concentrations between 4 and 8 wt%. The process involves co-extrusion of a clotrimazole-loaded PCL along with poly(ethylene oxide) (PEO) as a co-feed, with subsequent removal of PEO to isolate PCL-clotrimazole fibers. In vitro tests of the clotrimazole-containing fibers against the fungus Aspergillus fumigatus, Candida albicans, and Trichophyton mentagrophytes strains demonstrated good antifungal activity which was maintained for more than 3 weeks. An in vivo study using a mouse model showed the lowest tissue fungal burden for PCL-clotrimazole when compared to a PCL-only patch and untreated controls. Comparative studies were conducted with clotrimazole-containing PCL fibers fabricated by electrospinning. Our data showed that the co-extruded, clotrimazole-containing fibers maintain activity for longer times vs. electrospun samples. This, coupled with the much higher throughput of the co-extrusion process vs. electrospinning, renders this new approach very attractive for the fabrication of drug-releasing polymer fibers.

KEY WORDS

antifungal release clotrimazole co-extruded fiber poly(caprolactone) 

Notes

ACKNOWLEDGMENTS

The authors acknowledge funding from the National Science Foundation (NSF) Science and Technology Center, the Center for Layered Polymeric Systems (CLiPS), under Grant DMR-0423914, and the NSF under Grant CMMI-1335276. We are also grateful for discussions with Dr. Andrew Olah of CLiPS.

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Copyright information

© American Association of Pharmaceutical Scientists 2016

Authors and Affiliations

  • Mohammad Mofidfar
    • 1
  • Jia Wang
    • 1
  • Lisa Long
    • 2
    • 3
  • Christopher L. Hager
    • 2
    • 3
  • Chairut Vareechon
    • 4
  • Eric Pearlman
    • 5
  • Eric Baer
    • 1
  • Mahmoud Ghannoum
    • 2
    • 3
    Email author
  • Gary E. Wnek
    • 1
    Email author
  1. 1.Department of Macromolecular Science and EngineeringCase Western Reserve UniversityClevelandUSA
  2. 2.Department of Dermatology and Center for Medical MycologyCase Western Reserve UniversityClevelandUSA
  3. 3.University Hospitals Case Medical CenterClevelandUSA
  4. 4.Department of Ophthalmology and Visual ScienceCase Western Reserve UniversityClevelandUSA
  5. 5.Institute for Immunology, Physiology & Biophysics, School of MedicineUniversity of CaliforniaIrvineUSA

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