A Non-invasive Liquid Biopsy Screening of Urine-Derived Exosomes for miRNAs as Biomarkers in Endometrial Cancer Patients
Exosomes have great potential to serve as a source of diagnostic and prognostic biomarkers for endometrial cancer (EC). Urine-derived exosomes from patients with EC and patients with symptoms of EC, but without established EC, were used to evaluate a unique miRNA expression profile. Of the 84 miRNA studied, 57 were amplified in qPCR, suggesting the differential packaging of miRNA in exosomes. Further, hsa-miR-200c-3p was identified to be enriched the most. Various bioinformatics and in silico tools were used to evaluate the biological significance of hsa-miR-200c-3p in EC. We conclude that differential miRNA in exosomes can be utilized for discovery of biomarker signatures and EC diagnosis; hsa-miR-200c-3p is one such candidate. Urine-derived exosomes pave the way for the development of non-invasive biomarkers.
KEY WORDSexosomes endometrial cancer miRNA liquid biopsy biomarkers
The authors thank all the patients for providing the samples and the dedicated cancer center staff for assistance in sample collection. Editorial assistance from Ms. Kathy Kyler at the office of Vice President of Research, OUHSC, is appreciated. Rajagopal Ramesh is an Oklahoma TSET Research Scholar and holds the Jim and Christy Everest Endowed Chair in Cancer Developmental Therapeutics.
AS, KM, and RR conducted the studies and collected data; YDZ performed statistical analysis; AS, KM, RR, DND, YDZ, and RR conceived and designed the studies; AS and RR wrote the manuscript; AS, KM, RR, DND, YDZ, and RR critically analyzed and interpreted the data; AS, KM, RR, DND, YDZ, and RR critically reviewed, provided suggestions, and edited the manuscript; and RR supervised the project.
The work was supported in part by funds received from the Stephenson Cancer Center Seed Grant (RR), Presbyterian Health Foundation Seed Grant (RR), Presbyterian Health Foundation Bridge Grant (RR), Chapman Foundation, and Jim and Christy Everest Endowed Chair in Cancer Developmental Therapeutics (RR) at the University of Oklahoma Health Sciences Center.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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