Abstract
Salivary lysozyme levels were measured to determine the effect of stress on innate mucosal immunity. In the first study perceived stress levels of 39 participants were measured using a stress/arousal checklist questionnaire. Salivary lysozyme levels were found to show a significant negative correlation (r = −.477, p <.01) with the perceived stress level. In a second study salivary lysozyme concentrations were measured during periods of high and low stress in the form of an actual stressor—final year examinations. The concentration of lysozyme was found to be significantly (p < .05) lower in the sample taken before the examination when the students were awaiting entry into the examination hall compared to the levels after completion of all examinations. These results indicate that salivary lysozyme concentrations are sensitive to psychological stress and could be utilized as a potential marker in studies looking at the effects of stress on immunity. Because the usefulness of slgA as a marker has been questioned, the use of salivary lysozyrne as a noninvasive index of mucosal immunity warrants further investigation.
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Arnold, R. R., Pruitt, K. M., Cole, M. R., Adamson, J. M., & McGhee, J. R. (1979). Salivary antibacterial mechanisms in immunodeficiency. In I. Kleinberg, S. Ellison, & I. Mandel (Eds.), Saliva and dental caries (pp. 449–462). New York: Information Retrieval Inc.
Buckingham, J., Christian, H., Gillies, G., Philip, J. G., & Taylor, A. D. (1996). The HPA immune axis: In J. A. Marsh & M. D. Kendall (Eds.), The physiology of immunity (pp. 331–335). New York: CRC.
Calabrese, J. R., Kling, M. A., & Gold, P. W. (1987). Alterations in immunocompetence during stress, bereavement and depression: Focus on neuroendocrine regulation. The American Journal of Psychiatry, 144, 1123–1134.
Cox, T., & MacKay, C. (1985). The measurement of self reported stress and arousal. British Journal of Psychology, 76, 183–186.
Dorian, B., Garfinkel, P., Brown, G., Shore, A., Gladman, D., & Keystone, E. (1982). Aberrations in lymphocyte subpopulations and function during psychological stress. Clinical and Experimental Immunology, 50, 132–141.
Dupont, E., Huygen, K., Schandene, L., Vandercnrys, M., Palfliet, K., & Wybran, J. (1985). Influence of in vivo immunosuppressive drugs on production of lymphokines. Transplantation, 39, 143.
Falchuk, K. R., Perrotto, J. L., & Isselbacher, K. L. (1975). Serum lysozyme in Crohn’s disease and ulcerative colitis. New England Journal of Medicine, 292, 395–397.
Friedman, S. A., Mandel, I. D., & Herrera, M. S. (1983). Lysozyme and lactoferrin quantitation in crevicular fluid. Journal of Periodontology, 54, 347–350.
Germaine, G. R., & Tellefson, L. M. (1979). Simple and rapid procedure for the selective removal of lysozyme from human saliva. Infection and immunity, 26, 991–995.
Houldin, A. D., Lev, E., Prystowsky, M. B., Redei, E., & Lowery, B. J. (1991). Psychoneuroimmunology: A review of literature. Holistic Nursing Practice, 5, 10–21.
Jemmott, J. B., Borysenko, J. Z., Borysenko, M., McCleiiand, D. C., Chapman, R., Meyer, D., & Benson, H. (1983, June 25). Academic stress, power motivation and decrease in secretion rale of salivary secretory immunoglobulin A. The Lancet, 1400-1402.
Jolles, P., & Jolles, J. (1984). What’s new in lysozyme research? Molecular and Cellular Biochemistry, 63, 165–189.
Khansari, D. N., Murgo, A. J., & Faith, R. E. (1990). Effects of stress on the immune system. Immunology Today, 11, 170–175.
Kiecolt-Glaser, J. K., Speieher, C. E., Holliday, J. E.,& Glaser, R. (1984). Stress and the transformation of lymphocytes by Epstein-Barr virus. Journal of Behavioural Medicine, 7, 1–12.
Locke, S. E., Kraus, L., Lesserman, J., Hurst, M. W., Heisel, J. S., & Williams, R. M. (1984). Life change,psychiatric symptoms and natural killer cellactivity. Psychosomatic medicine, 46, 441–453.
Mouton, C., Fillion, L., Tawadros, E., & Tessier, R. (1989, Winter). Salivary IgA is a weak stress marker. Behavioural Medicine, 179-185.
Osserman, E. F., & Lawlor, D. P. (1966). Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukaemia, Journal of Experimental Medicine, 124, 921–949.
Perillie, P. E., Khan, K., & Finch, S. D. (1973). Serum lysozyme in pulmonary tuberculosis. American Journal of the Medical Sciences, 265, 297–302.
Pryzwansky, K. B., Martin, L. E., & Spitznagel, J. K. (1978). Immunocytochemical localisation of myeloperoxidase, lactoferrin, lysozyme and neutral proteases in human monocytes and neutrophilic granulocytes. Journal of Reticuloendothelial Society, 24, 295–310.
Reinhart, J., Nuest, D., & Ackerman, G. A. (1983). Corticosteroid alteration of human monocyte to macrophage differentiation. Journal of Immunology, 129, 1436–1440.
Rudney, J. D. (1989). Relationships between human parotid saliva lysozyme, lactoferrin, salivary peroxidase and sIgA in a large sample population. Archives of Oral Biology, 34, 499–506.
Van Rood, Y. R., Bogaards, M., Goulmy, E., & Houwelingen, H. C. (1993). The effects of stress and relaxation on the in vitro immune response in man: A meta analytic study. Journal of Behavioural Medicine, 16, 163–181.
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Perera, S., Uddin, M. & Hayes, J.A. Salivary lysozyme: A noninvasive marker for the study of the effects of stress on natural immunity. Int. J. Behav. Med. 4, 170–178 (1997). https://doi.org/10.1207/s15327558ijbm0402_5
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DOI: https://doi.org/10.1207/s15327558ijbm0402_5