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Anti-arthritic potential of latex of Alstonia scholaris (L.) R. Br.: an in silico and in vivo approach

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Abstract

Background

In the Dhemaji province of Assam, India, the latex of Alstonia scholaris (AS) is traditionally used as a topical agent for arthritis treatment. This study was designed for the first time to explore AS latex for chemical profiling, in vivo anti-arthritic activity and in silico molecular docking against cyclooxygenase (COX-2).

Methods

AS latex was chemically profiled by FTIR, HPTLC and LC-MS/MS. A complete Freund’s adjuvant (CFA)-induced arthritic rat model was used for the evaluation of anti-arthritic activity of AS latex. The compounds detected in AS latex were subjected to molecular docking to determine their ligand-binding affinities with COX-2.

Results

HPTLC fingerprinting and LC-MS/MS of AS latex confirmed the presence of potent anti-arthritic components such as quercetin, kaempferol, and ursolic acid. An in vivo study revealed that AS latex treatment restored arthritic parameters and controlled haematological and biochemical parameters in arthritic rats. AS latex prevented bone destruction, reduced tissue swelling and joint space narrowing in rats with arthritis. It reduced joint friction and made physical movements comfortable and pain-free in arthritic rats. An in silico study revealed that compounds in AS latex promoted better binding to the receptor protein COX-2. The results of the molecular docking studies supported in vivo statistics.

Conclusion

Based on studies performed in vivo and in silico, the results indicate that AS latex may be used as a potent novel anti-arthritic agent in treating rheumatoid arthritis and other inflammatory diseases.

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Acknowledgements

The authors are thankful to the Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh (Assam), India, and the Department of Herbal Science and Technology, ADP College, Nagaon (Assam), India, for providing the platform to conduct this work.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Drug Delivery Research Laboratory, Dibrugarh University, Dibrugarh, Assam, India

    Bapan Banik, Malay Kumar Das & Sanjoy Das

  2. Department of Chemical Engineering, National Chung Cheng University, Chiayi, Taiwan, 62102, ROC

    Sourav De

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Contributions

BB: plant material collection, chemical analysis, biological experiments, interpretation of the data, statistical analysis, manuscript writing. MKD: conception of the study, conceived, designed, and contributed to the formal analysis of the study, and reviewed/ edited the manuscript. SDAS: figure preparation, statistical analysis. SD: in silico docking study, editing of the manuscript. All authors have read the final manuscript and approved the submission.

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Correspondence to Malay Kumar Das.

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Protocols of the analysis were approved by the Institutional Animals Ethical Committee (IAEC) of Dibrugarh University, Dibrugarh-785004, Assam (IAEC Approval number: IAEC/DU/177, dated 27/08/2019). The guidelines of the “Committee for the Purpose of Control and Supervision of Experiments on Animals” (CPCSEA), Government of India, were followed.

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Banik, B., Das, M.K., Das, S. et al. Anti-arthritic potential of latex of Alstonia scholaris (L.) R. Br.: an in silico and in vivo approach. Nutrire 48, 40 (2023). https://doi.org/10.1186/s41110-023-00225-8

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