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Association between resting energy expenditure, diet and uncoupling protein 2 in obese women with normal and low resting energy expenditure

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Abstract

Purpose

Obesity is associated with increased risk for several metabolic diseases. Low Resting Energy Expenditure (REE) is considered a risk factor for weight gain and obesity. It was shown that diet composition can influence energy balance. Uncoupling Protein2 (UCP2) is a transporter protein presented in mitochondrial inner membrane which plays a role in cellular energy hemostasis. Our study was carried out to investigate the association between REE, diet and UCP2 in women with obesity in different REE.

Methods

A total forty nine subjects participated in the current study. Sixteen subjects with body mass index (BMI) > 30 and low REE, seventeen subjects with BMI > 30 and normal REE, and Sixteen non-obese subjects as control group. Subjects with measured REE less than 20% of their predicted REE were defined as low REE. Anthropometric, body composition parameters and REE were measured. Fasting blood UCP2 in Peripheral blood mononuclear cell (PBMC) and thyroid hormones were determined. Validated questionnaires for 24-h food records was used.

Results

There was a significant association between REE and protein intake in normal weight group (p-value = 0.03). In addition, there was a significant relationship between UCP2, protein and carbohydrate intake in group with obesity and low REE (p-value = 0.01, p-value < 0.01). After adjustment for age, fat-free mass and waist circumference, UCP2 was associated with measured REE in control group when compared to each group separately (95% CI, β = 0.42, p-value = 0.04). Furthermore there was a significant relationship between UCP2 and REE in three groups when we pooled (95% CI, β = 0.23, p-value = 0.04). Moreover, after adjustment for fat-free mass, we found, 0.15 decrease in the chance of low REE with the high level concentration of UCP2 in women with obesity (OR = 0.15, p-value = 0.04).

Conclusion

There was an association between REE, UCP2 and dietary intake (protein and carbohydrate). In addition REE was lower in some women with obesity and decreasing in the level of UCP2 protein may relate to this problem.

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Data Availability

Not applicable in this section.

Abbreviations

BMI:

Body Mass Index

FFM:

Fat Free Mass

FM:

Fat Mass

HFD:

High-fat diet

REE:

Resting Energy Expenditure

PBMCs:

Peripheral Blood Mononuclear Cells;

TSH:

Thyroid Stimulating Hormone

UCP:

Uncoupling Protein

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Acknowledgements

We would like to thank the Research Council of Tehran University of Medical Sciences (28767) for their financial support and all the subjects who participated in this study.

Funding

Research Council of Tehran University of Medical Sciences (28767).

Author information

Authors and Affiliations

  1. Department of Cellular-Molecular Nutrition, School of Nutrition Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran

    Zahra Taghadomi Masoumi & Hamideh Pishva

  2. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Eshraghian

  3. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Saeed Hosseini

Authors
  1. Zahra Taghadomi Masoumi
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  2. Mohammad Reza Eshraghian
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  3. Saeed Hosseini
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  4. Hamideh Pishva
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Contributions

HP contributed to conception of the idea, study design, ZT participated in performing study, SH provided assistance in selecting cases, ME helped with statistical analysis. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Hamideh Pishva.

Ethics declarations

Ethical Approval and Consent to participate

All procedures performed were in accordance with the ethics committee of Tehran University of Medical Sciences (TUMS), in compliance with the principles of the Declaration of Helsinki. All participants were informed of the nature of the study and gave a written informed consent.

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Not applicable.

Competing interests

All authors declare that they have no conflict of interests.

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Masoumi, Z.T., Eshraghian, M.R., Hosseini, S. et al. Association between resting energy expenditure, diet and uncoupling protein 2 in obese women with normal and low resting energy expenditure. Nutrire 48, 19 (2023). https://doi.org/10.1186/s41110-023-00202-1

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