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Vitamin B12 and coenzyme Q10 ameliorated alcohol-driven impairment of hematological parameters, inflammation, and organ damage in a mouse model

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Abstract

Introduction

Chronic alcohol consumption is associated with a myriad of negative physiological and biochemical changes in humans. Vitamin B12 and coenzyme Q10 (CoQ10) are novel antioxidants and anti-inflammatory agents.

Purpose

The objective of this study was to determine the impact of oral supplementation with vitamin B12 and CoQ10, in attenuating deleterious effects associated with alcohol exposure in Swiss albino mice.

Methods

Group one was normal control, the second group received 5 g/kg alcohol; the third group received 6 mg/kg b/w of vitamin B12 and 5 g/kg alcohol; the fourth group received 6 mg/kg b/w of vitamin B12, 200 mg/kg b/w CoQ10, and 5 g/kg alcohol, the fifth group 200 mg/kg b/w of CoQ10 and 5 g/kg alcohol.

Results

Oral administration of vitamin B12 and CoQ10 alone or in combination significantly ameliorated alcohol-induced impairment of hematological parameters and stabilized alcohol-induced alteration of the lipid profile. Notably, administration of either vitamin B12 or CoQ10 significantly blocked alcohol-induced depletion of reduced glutathione levels. Furthermore, vitamin B12 and CoQ10 stabilized the levels of pro-inflammatory cytokines (TNF-α and IFN-γ) when administered alone or in combination. Remarkably, the administration of CoQ10 and vitamin B12 significantly attenuated alcohol-induced liver and kidney inflammation and pathology.

Conclusion

Administration of either vitamin B12 or CoQ10 alone or in combination can protect from the toxic effects of chronic alcohol exposure.

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Data availability

All data generated and analyzed from this study are included in this manuscript or are available through the corresponding author upon request.

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Acknowledgements

The authors are indebted to The World Academy of Sciences and Kenya National Innovation Agency. Bio-reagents and chemicals purchased using funds from these organizations supported this research.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, Technical University of Kenya, P. O. Box 52428, Nairobi, 00200, Kenya

    Biwott Kipchumba, Victoria K. Mwaeni & James Nyabuga Nyariki

  2. Department of Pharmaceutical Sciences and Technology, Technical University of Kenya, P. O. Box 52428, Nairobi, 00200, Kenya

    Alfred Orina Isaac

  3. Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, P.O. Box, Nairobi, 43844-00100, Kenya

    Biwott Kipchumba, George Omwenga & Mathew Ngugi

  4. Department of Biophysics and Cell Biology, University of Debrecen, P.O. Box H-4032, Debrecen, Hungary

    Biwott Kipchumba

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Contributions

BK, VKM, and JNN performed experiments; BK, GO, and JNN analyzed and interpreted the experimental results; AOI provided the laboratory facility and helped in the designing of experimental procedures BK wrote the manuscript. MG, MG, AOI, and JNN edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to James Nyabuga Nyariki.

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The authors declare no competing interests.

Ethics approval

All experimental procedures and protocols involving mice were reviewed by the Institutional Review for approval Committee (IRC) of the Institute of Primate Research Karen, Kenya (ISERC/08/2017). All experiments were conducted in compliance with the recommendations of the Helsinki Declaration on guiding principles on the care and use of animals.

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The authors declare no competing interests.

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Kipchumba, B., Isaac, A.O., Mwaeni, V.K. et al. Vitamin B12 and coenzyme Q10 ameliorated alcohol-driven impairment of hematological parameters, inflammation, and organ damage in a mouse model. Nutrire 48, 13 (2023). https://doi.org/10.1186/s41110-023-00197-9

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  • DOI: https://doi.org/10.1186/s41110-023-00197-9

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