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Evaluation of modulatory effects of taurine in the aortic and myocardial tissue of nitroglycerin-induced tolerance Wistar rats

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Abstract

Purpose

Important underlying mechanisms of nitroglycerine tolerance development include oxidative stress and endothelial dysfunction, and there is paucity of information on how to reduce the tolerance during long-term administration. Taurine, a sulfonic amino acid, was reported to possess antioxidant, cardio-regulatory, neuro-modulatory, and membrane-stabilizing effect. The present study was designed to investigate the potential ability of taurine to prevent nitroglycerine-induced tolerance.

Methods

The effect of taurine on nitroglycerin-induced tolerance was investigated in endothelium intact and endothelium-denuded aortic ring preparations. In the in vivo study, male Wistar rats were pre-treated with taurine for ten days and co-treated with nitroglycerin (GTN) 50 mg/kg for 3 days. Then, the aortic ring was harvested and tested in vitro for GTN tolerance. The serum was used to test for oxidative stress parameter (malondialdehyde, reduced glutathione (GSH), catalase (CAT)).

Results

Taurine (20 mM) co-incubated with GTN significantly augmented vasodilatory response to nitroglycerin (GTN) in endothelial-denuded aortic rings. Pre-treatment with taurine (100 and 200 mg/kg) also ameliorated GTN (50 mg/kg)-induced tolerance in isolated aortic ring in rats. Also, taurine (100 and 200 mg/kg) significantly (p < 0.05) increased serum nitrite, GSH, and CAT levels while reducing malondialdehyde (MDA) concentration.

Conclusion

Taurine could prevent nitroglycerin-induced tolerance by increasing serum nitric oxide level and decreasing oxidative stress.

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Data availability

All data and material for this study will be provided upon request.

Code availability

Not Applicable.

Abbreviations

MDA:

Malondialdehyde

GSH:

Reduced glutathione

CAT:

Catalase

ROS:

Reactive oxygen species

GTN:

Nitroglycerine

ACh:

Acetylcholine

NE:

Norepinephrine

NO:

Nitric oxide

LPO:

Lipid peroxidation

NO2 − :

Nitrite

GSH-Px:

Glutathione peroxidase

NADPH:

Nicotinamide adenine dinucleotide phosphate

cyt P450:

Cytochrome P450

L-NAME:

N-nitro-L-arginine methylester

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, University of Ibadan, Ibadan, Nigeria

    Oluseye Odebiyi, Joseph Badejo, Abayomi Ajayi, Olugbenga Iwalewa & Oluwole Fagbemi

  2. Department of Pharmacology and Therapeutics, Bowen University, Iwo, Nigeria

    Babatunde Alabi

Authors
  1. Oluseye Odebiyi
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  2. Joseph Badejo
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  3. Babatunde Alabi
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  4. Abayomi Ajayi
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  5. Olugbenga Iwalewa
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  6. Oluwole Fagbemi
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Contributions

This study was carried out in collaboration among all authors. The following authors OO, JAB, and OSF designed the study. OO, BAA, AAM, and JAB performed the statistical analysis, wrote the protocol and the first draft of the manuscript. EOI and OSF managed the managed literature searches and supervised the experiment. All authors read and approve the final manuscript.

Corresponding author

Correspondence to Joseph Badejo.

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Conflict of interest

The authors declare no competing interests.

Ethical approval

All of the experiments were conducted according to the approved guidelines set by the University of Ibadan Animal Care and Use Research Ethics Committee (UI-ACUREC), which is in agreement with the “Guide for the Care and Use of Laboratory Animals” prepared by the National Academy of Science and published by the National Institutes of Health. The ethical approval number assigned for animal use in this study by UI-ACUREC was UI-ACUREC/17/0071.

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Odebiyi, O., Badejo, J., Alabi, B. et al. Evaluation of modulatory effects of taurine in the aortic and myocardial tissue of nitroglycerin-induced tolerance Wistar rats. Nutrire 46, 12 (2021). https://doi.org/10.1186/s41110-021-00141-9

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