Antioxidant and antibacterial activity of Ipomoea mauritiana Jacq.: a traditionally used medicinal plant in Bangladesh

Abstract

Background

In an attempt to explore the scientific basis for the pharmacological benefits the antioxidant and antimicrobial activities of Ipomoea mauritiana whole plant methanol extract were assessed.

Methods

The total phenolic and flavonoid content were determined using standard method while antioxidant activity was determined by DPPH free radical scavenging activity method. The antimicrobial activity was evaluated by disk diffusion method and compared with standard kanamycin (30 μg/disc).

Results

The results revealed that I. mauritiana extract contains tannin, saponin, terpenoids, alkaloid and flavonoids. In DPPH, ascorbic acid and extract showed highest scavenging activity and it was 90.96% at concentration 800 μg/mL and I. mauritiana methanol extract showed 72.28% at a concentration of 800 μg/mL. The extract was able to reduce the stable free radical DPPH with an IC50 of 275.084 μg/mL while that of ascorbic acid was 230.09 μg/mL. Total phenolic constituent of the extract was 59.302 ± 3.289 mg/g as gallic acid equivalent. The flavonoid content of methanolic extract of I. mauritiana was 27.212 mg of QE/g. In case of antimicrobial screening, crude extracts of I. mauritiana showed notable antibacterial activity against tested microorganisms. The extract and standard showed the highest mean zone of inhibition ranging from 13 to 19 mm and 37 to 42 mm, respectively at a concentration of 400 μg /disc and against the gram positive bacteria (Bacillus cereus-19.25 mm) showed highest zone of inhibition.

Conclusions

The results indicate that I. mauritiana possesses considerable antioxidant and antimicrobial activity.

Introduction

Ipomoea mauritiana (I. mauritiana) Jacq. (Synonyms: Ipomoea digitata; Ipomoea eriosperma; Ipomoea paniculata; Convolvulus paniculatus) is a vine of the Convolvulaceae family. The plant has ethnomedicinal importance and found in many parts of the world. In Ayurvedic industries, I. mauritiana is popularly used as “Vidari” instead of the permitted raw drug Pueraria tuberose (Roxb. ex Willd). The Ayurvedic Pharmacopoeia of India correlates I. mauritiana as Kshiravidari. Tubers are used in almost 45 Ayurvedic formulations, and in many instances also used as single drug. This is also an important component of the popular ayurvedic formulation Chyavanaprasha [1]. Traditionally the tubers are also extensively used by local communities in different parts of Bangladesh to treat various ailments including pain in spinal cord, to increase breast milk, body strength and sperm count, to control tuberculosis, as blood purifier in jaundice and to treat biliary disorders [2,3,4,5]. The leaves are also used to treat leucorrhea and diabetes with obesity and in infrequent urination by the folk medicine practitioners of Kurigram and Jhalokati districts of Bangladesh [6].

The pharmacological investigations reported analgesic, hypoglycemic, hypocholesterolemic, and hepatoprotective activities of I. mauritiana tuber root [7, 8]. Additionally, the antibacterial activity against Escherichia coli which caused catheter associated urinary tract infections has also been reported by Pavan [9]. A number of phytochemical have been isolated from I. mauritiana tuber root methanol extracts which includes taraxerol, taraxerol acetate, β-sitosterol, scopoletin and 7-O-β-D-glycopyranosylscopoletin (scopolin) [10]. Literature showed that, scopoletin isolated from Lasianthus lucidus possessed antibacterial activity against Pseudomonus aerigenosa [11]. Plants are major source of natural antioxidants that can serve as possible drug candidates for different chronic ailments such as neurological diseases, swelling and diabetes [12, 13]. The wide range of potential benefits of natural antioxidants has increased the curiosity largely in medicine to ascertain new molecules to combat oxidative stress caused by free radicals [13].

Infective microbes are one of the major public health threats causing morbidity and mortality worldwide [14]. Several life-threatening microorganisms have now become resistant to many commercial antibiotics [15]. Novel, safe, potent and wide-spectrum antimicrobial molecules are therefore urgently needed [16]. Even though the plant has been widely used as traditional medicine, however there is inadequate scientific evidence on the potential benefits of the whole plant. Therefore, the current study was designed to determine the antioxidant and antimicrobial activities of I. mauritiana whole plant methanol extract.

Materials and methods

Plant collection

I. mauritiana was collected from Tangail district of Bangladesh in July 2018 and was identified by Bangladesh National Herbarium. The collected plants (as a whole) were cleaned using tap water to remove the soil and washed with distilled water followed by shade drying. The dried plants were grounded into a fine powder.

Plant extract preparation

Powdered whole plant material having a weight of about 50 g were taken in one amber glass container and soaked in 500 mL methanol, for 3 days with occasional shaking and stirring. The extractive was then filtered using Whatman filter paper number 1 to attain a clear filtrate. The filtrate extract was concentrated and dried in vacuum at 40 °C and stored at 4 °C until further use.

Preliminary phytochemical screening

The presence of different phytochemicals such as alkaloid, steroids, glycosides, flavonoid, tannins, saponin, and terpenoids in crude methanol extract was determined following standard methods [17, 18]. Any color change or precipitation formation indicated positive reactions to these tests.

Test for steroids and Triterpenoids

In the extract solution, few drops of acetic anhydride added and boiled. After cooling, concentrated sulphuric acid was slowly poured into the test tube. Formation of violet to blue or green color (Liebermann–Burchard reaction for steroid) indicated the positive reaction. Formation of green color of the upper layer and reddish- brown color in the bottom layer confirmed a positive test for steroids and terpenoids respectively [17, 18].

Test for glycosides

The plant extract solution was dissolved in a mixture of glacial acetic acid (5%) and Ferric chloride solution and one or two drop of concentrated sulphuric acid was added. A brown ring or violet ring or greenish ring formation coloration indicated a positive test for glycosides [19].

One mL of distilled water was added to 10 drops of the extract dissolved in isopropyl alcohol (20 mg/mL) in a test-tube. The tube was shaken vigorously and observed for persistent froth formation and allowed to stand for 10 min. A positive result indicate a forth formation which is stable as long as 15 min [19].

Test for alkaloids

Few drops of Mayer’s reagent (potassium mercuric iodide solution) was mixed with I. mauritiana extract solution. A yellowish-white like precipitation indicates a positive reaction for the presence of such metabolites [19].

Ferric chloride test for tannins

The plant extract solution of 10 mg was dissolved in ethanol (1 mL). Then distilled water (2 mL) was added followed by 4 drops of ferric chloride aqueous solution (10% w/v). The occurrence of phenols was confirmed by the development of dark blue or green color [20].

Total phenolic content

Total phenolic content of I. mauritiana extract was measured using a Folin-Ciocalteu colorimetric method spectrophotometrically as described by Idowu [21] with slight modification. Gallic acid was used as standard (concentration: 6.25–200 μg/mL) while I. mauritiana whole plant extract was 200 μg/mL. Firstly, 1 mL of the extract or standard gallic acid solution was used in screw cap tube and 5 mL of Folin-Ciocalteu reagent was added. Then, 4 mL (7.5%) of anhydrous sodium carbonate was added followed by 30 in incubation at 40 °C. The vehicle solvent was used as blank solution. UV absorbance was taken with a UV–VIS spectrophotometer (Shimadzu, Japan) at 765 nm. Total phenolic content was calculated as gallic acid equivalent (GAE) using the equation as follows:

$$ \mathrm{C}=\left(\mathrm{c}\times \mathrm{V}\right)/\mathrm{m} $$

Where C = Total phenolics (mg/g plant extract in GAE), c = concentration of sample obtained from calibration curve (mg/mL), V = volume of the sample, and m = sample weight (g).

Total flavonoid content

To determine the total flavonoid content, the method of Rahman [22] was adopted, quercetin was the standard and different concentrations were used (6.25–200 μg/mL) while I.mauritiana methanol extract was 200 μg/mL. Initially, in a volume of 1 mL of extract or 1 mL quercetin solution of different concentrations, 3 mL of methanol was added. After that, 200 μL of 10% aluminum chloride and 200 μL of 1 M potassium acetate solution were added followed by the addition of 5.6 mL distilled water. Then the mixture incubated at room temperature for 30 min. Absorbance at 415 nm was recorded against the blank (water). The total flavonoid content was calculated as per the formulae bellow:

$$ \mathrm{C}=\left(\mathrm{c}\times \mathrm{V}\right)/\mathrm{m} $$

Where C = Total flavonoid content (mg/g plant extract in Quercetin), c = concentration of sample obtained from calibration curve (mg/mL), V = volume of sample, and, m = sample weight (g). Measurement was taken triplicate.

DPPH radical scavenging activity

To determine the antioxidant activity, the method of described by Braca [23] was adopted with slight modification. At first, 100 μL of plant extract and different concentrations of standard (ascorbic acid) were taken in test tubes. Then, 3 mL of 0.004% DPPH (2,2-diphenyl-1-picrylhydrazyl) solution in ethanol was added to each test tube followed by 30 min incubation. Later absorbance was taken at 517 nm spectrophotometically against methanol (blank). The absorbance values were used to calculate the percentage of antioxidant activity (% inhibition) using the formula below:

$$ \%\mathrm{Scavenging}=\left\{\left({\mathrm{A}}_0-{\mathrm{A}}_1\right)/{\mathrm{A}}_0\right\}\times 100. $$

Where A0 = Absorbance of DPPH solution only; A1 = Absorbance in the presence of test sample and standard ascorbic acid in DPPH solution. Measurement was taken triplicate.

Antimicrobial activity of I. mauritiana

Microorganisms

Four gram-positive and six gram-negative bacteria were selected as test organisms. Gram positive; Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus and Gram negative; Escherichia coli, Salmonella typhi, Salmonella paratyphi, Pseudomonas aeruginosa, Vibrio parahemolyticus and Shigella dysenteriae were used for antimicrobial activity test. Each of the stock cultures was collected as pure culture from the Institute of Nutrition and Food Science (INFS), University of Dhaka, Bangladesh.

Antibacterial activity

Disk diffusion method was used to perform the antibacterial test [24]. The sample solutions (400 and 200 μg/mL) were made by dissolving in methanol. Sterilized and dried filter paper discs (6 mm diameter) were then soaked in the crude extracts (400 and 200 μg/mL/disc) using micropipette and the solvent residue evaporated to dryness. Kanamycin (30 μg/disc) and blank discs (soaked with methanol followed by evaporation) were positive and negative control, respectively. The plates were inverted and refrigerated at 4 °C for 24 h for maximum diffusion. The following day plates were placed in the incubator at 37 °C for another 24 h for optimum bacterial growth. The antimicrobial activity was observed with inhibited growth of the microorganisms giving a clear, distinct zone of inhibition around the discs. Finally, the diameter of zone of inhibition was measured. The data was obtained from three individual experiments (n = 3).

Statistical analysis

A statistical analysis was used to interpret the antimicrobial and antioxidant results. The experiment was conducted in completely randomized design with 3 replicates. The results are presented as means ±standard error of means using MS excel [25].

Result and discussion

The results of preliminary phytochemical screening of I. mauritiana are presented in Table 1. Results revealed that the methanolic extract of I. mauritiana contains terpenoids, saponins, flavonoids, steroids, and alkaloids. The presence of these classes of compounds of this plant could be responsible for its various medicinal uses [26, 27].

Table 1 Phytochemical screening of I. mauritiana

On the other hand, the total phenolic content of I. mauritiana was evaluated as expressed by gallic acid equivalents per gram of extract which was found as 59.302 ± 3.289 mg of GAE/g. The value was obtained from regression equation of the calibration curve (y = 0.004x + 0.105; r2 = 0.992) as shown in Fig. 1. Subsequently, the total flavonoid content of methanol extract of I. mauritiana was 27.212 ± 0.51 mg of QE/g which was obtained from the regression equation (y = 0.003x + 0.023; r2 = 0.995) as shown in Fig. 2.

Fig. 1
figure1

Concentration-response curve of gallic acid standard at 765 nm

Fig. 2
figure2

Concentration–response curve of quercetin standard at 415 nm

Similarly, DPPH free radical scavenging activity of I. mauritiana and ascorbic acid is shown in the Table 2. Both ascorbic acid and I. mauritiana methanolic extract showed dose dependent activity. Among the eight different concentrations (6.25, 12.5, 25, 50, 100, 200, 400 and 800 μg/mL) ascorbic acid showed 14.05 ± 1.64, 16.66 ± 1.55, 20.47 ± 1.47, 27.20 ± 1.64, 42.76 ± 1.64, 74.89 ± 1.63, 87.82 ± 1.73 and 90.96 ± 1.64% scavenging activity respectively while, I. mauritiana methanol extract showed 20.68 ± 1.15, 26.50 ± 1.64, 31.92 ± 1.80, 38.45 ± 1.89, 48.99 ± 1.73, 59.03 ± 1.86, 66.86 ± 1.78 and 72.28 ± 1.8% scavenging activity respectively. In addition, the IC50 of ascorbic acid and methanolic extract were 230.09 μg/mL and 275.084 μg/mL respectively as shown in Table 2. The DPPH scavenging activity of I. mauritiana methanol extract was lesser than that of standard ascorbic acid. The IC50 value denotes the minimum sample concentration required to scavenge 50% free radical. The antiradical activity of the extract may be due to their high content of phenols, as polyphones which contribute to the antioxidant activity in living systems by chelation and electron transfer or hydrogen donating ability of hydroxyl groups in ortho- and para- positions thus neutralizing the free radicals [28, 29].

Table 2 DPPH free radical scavenging activity of ascorbic acid and I. mauritiana extract

The results of antibacterial activity of the extract and standard kanamycin with respect to each of the test organisms are presented in Table 3. The mean zone of inhibition of kanamycin was between 37 to 42 mm and the extract which was between 13 to 19 mm, respectively at a concentration of 400 μg /disc. Zone of inhibition of standard was larger than extract. The extract showed the highest zone of inhibition against the gram positive; Bacillus cereus (19.25 ± 1.020 mm) and Sarcina lutea (18.5 ± 0.0813 mm) and gram negative Shigella boydii (18.25 ± 3.06 mm) and had no activity against Escherichia coli. Gram positive strains were more sensitive to the extract than the gram negative ones. However, the extract had the lowest antibacterial effect to Pseudomonas aureus. These results indicate that I. mauritiana extract is active against both bacterial strains as compared to standard antibiotic, kanamycin.

Table 3 Antibacterial activity of I. mauritiana using the disk diffusion method

Plant has been a very significant source of medicine for decades and many plants have been tested for their potential bioactive compounds [30].

Free radicals are significant contributor in many pathological manifestations. Antioxidants can maintain good health and protect, either by counteracting these free radicals or defending the body’s antioxidant defense mechanisms. Plants and herbs, being the potential sources for a number of antioxidants in addition to their therapeutic properties, have appealed the concern of scientific community to meet up the growing interest for raw materials with potential natural antioxidant compounds. Phenolic or polyphenolic compounds are one of the major classes of compounds having strong antioxidant properties. The antioxidative action of phenolic compounds is primarily due to their redox properties, [31, 32] which can hydrolyze and neutralize free radicals, purify singlet and triplet oxygen, or decompose peroxides.

On the other hand, ongoing demand for new antibiotic is present due to the sustained emergence of infection-resistant strains. About 80% of the drugs available in many developing countries originated from medicinal plants. In the industries of many countries plants comprise the raw material for processes that synthesizes pure chemical derivatives [33].

In the present study, prominent antioxidant activity was observed for the whole plant extract of I. mauritiana with potential antibacterial activity against a number of Gram (+) ve and Gram (−) ve bacteria. Different parts of the plant may vary with their antioxidant activity. For instance, a study revealed that, the root and stem bark, leaf and flower parts of Tabebiua pallida methanol extract were tested for antioxidant activity where the leaf extract was found to show highest antioxidative and free radical scavenging property which was also rich in total phenols and flavonoid contents [34]. The current study thus was conducted with the whole plant of I. mauritiana. It is clear from the results that, total phenolic and flavonoid contents and DPPH scavenging activity signifies the potential bioactivity of I. mauritiana. This result is consistent with the report of Sulaiman [35]. Moreover, phenolic compounds demonstrates potential antibacterial activity. Rahman and collegues stated that, 3,4-dihydroxybenzoic acid obtained from Cananga odorata was active against a number of Gram (+) and Gram (−) bacteria [36].

In the same way, the antibacterial activity of I. mauritiana against gram positive bacteria such as Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus and gram negative bacteria such as Escherichia coli, Salmonella typhi, Salmonella paratyphi, Pseudomonas aeruginosa, Vibrio parahemolyticus and Shigella dysenteriae was evaluated. These are important pathogens and can rapidly develop antibiotic resistance as antibiotic use increases. However, a previous report stated that, the leaf freeze dried extract of I. mauritiana did not show any activity against Streptococcus mutans, S. mitis, Staphylococcus aureus, and a fungi Candida albicans in both agar disk and agar well diffusion tests [37]. This study reveals the promise of antibacterial activity of I. mauritiana against gram positive bacteria in particular.

Conclusion

The results demonstrated that the methanolic extract of I. mauritiana Jacq shown antioxidant and antimicrobial activity. These findings could be a scientific evidence to use this plant as a potential source of antioxidant and antibacterial agents. However, future studies are necessary to determine the mechanisms of these pharmacologic properties. Moreover, phytochemicals characterization of possible bioactive compounds in I. mauritiana Jacq is also required.

Availability of data and materials

All data generated and analyzed are present in this manuscript.

References

  1. 1.

    Devaiah K, Paranthaman S. Development of randomly amplified polymorphic DNA based SCAR marker for identification of Ipomoea mauritiana Jacq (Convolvulaceae). Evid Based Comple Alt Med. 2011;868720:1–6.

    Google Scholar 

  2. 2.

    Anzumi H, Rahman S, Islam MA, Rahmatullah M. Uncommon medicinal plant formulations used by a folk medicinal practitioner in Naogaon district, Bangladesh. World J Pharm Pharm Sci. 2014;3(12):176–88.

    Google Scholar 

  3. 3.

    Azad AK, Mahmud MR, Parvin A, Chakrabortty A, Akter F, Moury SI, Anny IP, Tarannom SR, Joy SK, Chowdhury SY, Akter S, Rahmatullah M. Ethnomedicinal surveys in two Mouzas of Kurigram district, Bangladesh. World J Pharm Pharm Sci. 2014;3(10):1607–20.

    Google Scholar 

  4. 4.

    Jahan N, Khan A, Hasan MN, Hossain MU, Das U, Sultana S, Rahmatullah M. Ethnomedicinal plants of fifteen clans of the Garo tribal community of Madhupur in Tangail district, Bangladesh. Am Eur J Sustain Agric. 2013;7:188–95.

    Google Scholar 

  5. 5.

    Walid R, Suvro KFA, Harun-or-Rashid M, Mukti M, Rahman S, Rahmatullah M. Ethnomedicinal plants of folk medicinal practitioners of two villages in Bagerhat district of Bangladesh. Am Eur J Sustain Agric. 2013;7(2):61–74.

    Google Scholar 

  6. 6.

    Rahmatullah M, Nuruzzaman M, Hossan MS, Khatun MA, Rahman MM, Jamal F, Harun-Or-Rashid M, Nasrin D, Seraj S, Jahan R. An ethnomedicinal survey of folk medicinal practitioners of Shitolpara village, Jhalokati district, Bangladesh. Adv Nat Appl Sci. 2010;4(1):85–92.

    Google Scholar 

  7. 7.

    Islam S, Ahmed MR, Al-Mahamud R. Callus extract of Ipomoea mauritiana show analgesic and antihyperglycemic activity in Swiss albino mice. J Appl Pharm Sci. 2015;5(10):44–7.

    Article  Google Scholar 

  8. 8.

    Moushumi JS. Hypoglycemic, hypocholesterolemic and hypotriglyceridemic activity of tuber roots of Ipomoea mauritiana Jacq (Convolvulaceae) when administered to rats. Adv Nat Appl Sci. 2010;4(2):174–6.

    Google Scholar 

  9. 9.

    Pavan HV, Murthy DSM, Devi DNL. Isolation and identification of urinary catheter associated bacteria and study of in vitro antibacterial activity of methanolic and petroleum ether leaf extracts of Ipomoea mauritiana Jacq against bacteria isolated from urinary catheters. Int J Herb Med. 2017;5(5):216–20.

    Google Scholar 

  10. 10.

    Dharmaratne HRW, Jayasinghe ULB, Weerawardhena WDPP, Herath HMTB, Fujimoto Y. Chemical investigation of Ipomoea mauritiana. ACGC Chem Res Commun. 1997;6:39–41.

    CAS  Google Scholar 

  11. 11.

    Tiwtawat N, Markus B, Henrik B, Kwankamol T, Wichai S, Srunya V. Scopoletin from Lasianthus lucidus Blume (Rubiaceae): a potential antimicrobial against multidrug-resistant Pseudomonas aeruginosa. J Appl Pharm Sci. 2018;8:001–6.

    Google Scholar 

  12. 12.

    Venkatesan A, Kathirvel A, Prakash S. Sujatha V, antioxidant, antibacterial activities and identification of bioactive compounds from Terminalia chebula bark extracts. Free Radic Ant. 2017;7(1):44–50.

    Google Scholar 

  13. 13.

    Paliwal SK, Sati B, Faujdar S, Sharma S. Antioxidant and antibacterial activities of various extracts of Inula cuspidate (CB) Clarke stem Beni-Suef. Univ J Basic Appl Sci. 2017;6(2):97–105.

    Google Scholar 

  14. 14.

    Natarajan D, Shivakumar MS, Srinivasan R. Antibacterial activity of leaf extracts of Biophytum sensitivum (L.) DC. J Pharm Sci Res. 2010;2(11):717–20.

    Google Scholar 

  15. 15.

    Srinivasan R, Aruna A, Manigandan K, Pugazhendhi A, Kim M, Shivakumar MS, Natarajan D. Phytochemical, antioxidant, antimicrobial and antiproliferative potential of Elaeagnus indica. Biocatal Agric Biotechnol. 2019;20:101265.

    Article  Google Scholar 

  16. 16.

    Srinivasan R, Natarajan D, Shivakumar MS. Antimicrobial and GC-MS analysis of Memecylon edule leaf extracts. Inter J Cur Res. 2014;5:1–13.

    Google Scholar 

  17. 17.

    Dev UK, Hossain MT, Islam MZ. Phytochemical investigation, antioxidant activity and anthelmintic activity of Mikania micrantha leaves. World J Pharm Res. 2015;4(5):121–33.

    Google Scholar 

  18. 18.

    Hossain MT. Antioxidant, cytotoxicity, membrane stabilization and anthelmintic activity of ethanolic extract of Sarcochlamys pulcherrima leaves. Int J Green Herbal Chem. 2015;4:274–83.

    CAS  Google Scholar 

  19. 19.

    Bhandary SK, Suchetha KN, Bhat VS, Sharmila KP, Bekal MP. Preliminary phytochemical screening of various extracts of Punica granatum peel whole fruit and seeds. Nitte Univ J Health Sci. 2012;2(4):35–8.

    Google Scholar 

  20. 20.

    María R, Shirley M, Xavier C, Jaime S, David V, Rosa S, Jodie D. Preliminary phytochemical screening, total phenolic content and antibacterial activity of thirteen native species from Guayas province. J King Saud Univ Sci. 2018;30(4):500–5.

    Article  Google Scholar 

  21. 21.

    Sagbo IJ, Afolayan AJ, Bradley G. Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria. Asian Pac J Trop Biomed. 2017;7(9):817–25.

    Article  Google Scholar 

  22. 22.

    Rahman MA, Chowdhury JMKH, Aklima J, Azadi MA. Leea macrophylla Roxb leaf extract potentially helps normalize islet of β-cells damaged in STZ-induced albino rats. Food Sci Nutr. 2018;6(4):943–52.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. 23.

    Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia terapotensis. J Nat Prod. 2001;64(7):892–5.

    Article  CAS  PubMed  Google Scholar 

  24. 24.

    Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol. 1996;45:493–6.

    Article  Google Scholar 

  25. 25.

    Ansar M, Gulam M. Phenolic contents, antimicrobial and antioxidant activity of Olea ferruginea Royle (Oleaceae). BMC Complement Alt Med. 2018;18:173.

    Article  CAS  Google Scholar 

  26. 26.

    Asolkar LV, Chopra RN. Second supplement to glossary of Indian medicinal plants with active principles. Publications & Information Directorate; 1992.

  27. 27.

    Mishra SS, Datta KC. A preliminary pharmacological study of Ipomoea digitata Linn. Indian J Med Res. 1962;50:43–5.

    PubMed  CAS  Google Scholar 

  28. 28.

    Oyaizu M. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Exp Med. 1986;44(6):307–15.

    CAS  Google Scholar 

  29. 29.

    Lapornik A, Prosek M, Wondra GA. Comparision of extracts prepared from plant byproducts using different solvents and extraction time. J Food Eng. 2005;71(2):214–22.

    Article  Google Scholar 

  30. 30.

    Rosy BA. Joseph H, Rosalie. Phytochemical, pharmacognostical, antimicrobial activity of Indigofera spalathoids Vahl (Fabaceae). Int J Biol Technol. 2010;1:12–5.

    Google Scholar 

  31. 31.

    Galato D, Ckless K, Susin MF, Giacomelli C, Ribeiro-do-Valle RM, Spinelli A. Antioxidant capacity of phenolic and related compounds: correlation among electrochemical, visible spectroscopy methods and structure-antioxidant activity. Redox Rep. 2001;6(4):243–50.

    Article  CAS  PubMed  Google Scholar 

  32. 32.

    Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agr Food Chem. 2001;49(11):5165–70.

    Article  CAS  Google Scholar 

  33. 33.

    Penso G. The role of WHO in the selection and characterisation of medicinal plants. J Ethnopharmacol. 1980;2(2):183–8.

    Article  CAS  PubMed  Google Scholar 

  34. 34.

    Rahman MM, Islam MB, Biswas M, Alam KAHM. In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res Notes. 2015;8:621.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. 35.

    Sulaiman C, Sivadasan PG, Balachandran I. Identification of phenolic antioxidants in Ipomoea mauritiana jacq using spectrophotometric and mass spectroscopic studies. Avicenna J Phytomed. 2014;4(2):89–96.

    PubMed  PubMed Central  CAS  Google Scholar 

  36. 36.

    Rahman MM, Lopa SS, Sadik G, Rashid H, Islam R, Khondkar P, Alam KAHM, Rashid A. Antibacterial and cytotoxic compounds from the bark of Cananga odorata. Fitoterapia. 2005;76:758–61.

    Article  CAS  PubMed  Google Scholar 

  37. 37.

    Pochapski MT, Fosquiera EC, Esmerino LA, dos Santos EB, Farago PV, Santos FA, et al. Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves' extract from Ipomoea batatas (L.) lam. Phcog Mag. 2011;7:165–70.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the Institute of Nutrition and Food Science (INFS), University of Dhaka, for generously providing the test organisms.

Funding

None.

Author information

Affiliations

Authors

Contributions

IA performed the extraction and antioxidant study and analyzed the data. MSF performed the antibacterial study. MSF and RM drafted the manuscript and MFFMA edited the manuscript. AKMMH planned and supervised the work and finalized the manuscript. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Moyeenul Huq.

Ethics declarations

Ethics approval and consent participate

None.

Consent for publication

Not applicable.

Competing interests

Authors have no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Alam, I., Forid, S., Roney, M. et al. Antioxidant and antibacterial activity of Ipomoea mauritiana Jacq.: a traditionally used medicinal plant in Bangladesh. Clin Phytosci 6, 35 (2020). https://doi.org/10.1186/s40816-020-00185-w

Download citation

Keywords

  • Ipomoea mauritiana
  • Antioxidant activity
  • Antimicrobial activity