Epigenome-wide DNA methylation profiling of periprostatic adipose tissue in prostate cancer patients with excess adiposity—a pilot study
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Abstract
Background
Periprostatic adipose tissue (PPAT) has been recognized to associate with prostate cancer (PCa) aggressiveness and progression. Here, we sought to investigate whether excess adiposity modulates the methylome of PPAT in PCa patients. DNA methylation profiling was performed in PPAT from obese/overweight (OB/OW, BMI > 25 kg m−2) and normal weight (NW, BMI < 25 kg m−2) PCa patients. Significant differences in methylated CpGs between OB/OW and NW groups were inferred by statistical modeling.
Results
Five thousand five hundred twenty-six differentially methylated CpGs were identified between OB/OW and NW PCa patients with 90.2% hypermethylated. Four hundred eighty-three of these CpGs were found to be located at both promoters and CpG islands, whereas the representing 412 genes were found to be involved in pluripotency of stem cells, fatty acid metabolism, and many other biological processes; 14 of these genes, particularly FADS1, MOGAT1, and PCYT2, with promoter hypermethylation presented with significantly decreased gene expression in matched samples. Additionally, 38 genes were correlated with antigen processing and presentation of endogenous antigen via MHC class I, which might result in fatty acid accumulation in PPAT and tumor immune evasion.
Conclusions
Results showed that the whole epigenome methylation profiles of PPAT were significantly different in OB/OW compared to normal weight PCa patients. The epigenetic variation associated with excess adiposity likely resulted in altered lipid metabolism and immune dysregulation, contributing towards unfavorable PCa microenvironment, thus warranting further validation studies in larger samples.
Keywords
DNA methylation Periprostatic adipose tissue Obesity Prostate cancer MicroenvironmentAbbreviations
- AA
Arachidonic acid
- ACADM
Aliases MCAD, medium-chain acyl-CoA dehydrogenase
- BH
Benjamin and Hochberg
- BMI
Body mass index
- CPT1B
Carnitine palmitoyltransferase 1B
- CPT1C
Carnitine palmitoyltransferase 1C
- CTL2
Choline transporter-like protein 2
- DAG
Diacylglycerol
- DGAT
Diacylglycerol O-acyltransferase
- DGAT2
Diacylglycerol O-acyltransferase 2
- DGEs
Differential gene expressions
- DGLA
Dihomo-gamma-linoleic acid
- DMCs
Differentially methylated CpG sites
- DMRs
Differentially methylated regions
- EPA
Eicosapentaenoic acid
- ER
Endoplasmic reticulum
- EWAS
Epigenetic-wide Association Studies
- FADS1
Fatty acid desaturase 1
- FDR
False discovery rate
- GO
Gene ontology
- GWAS
Genome-wide Association Studies
- HUFA
Highly unsaturated fatty acid
- KEGG
Kyoto Encyclopedia of Genes and Genomes
- LCFA
Long-chain fatty acids
- limma
Linear models for microarray and RNA-seq data
- MAG
Monoacylglycerol
- MCFA
Medium-chain fatty acid
- MGAT1
Aliases for MOGAT1
- MHC
Major histocompatibility complex
- MOGAT
Monoacylglycerol O-acyltransferase
- MOGAT1
Monoacylglycerol O-acyltransferase 1
- MOGAT3
Monoacylglycerol O-acyltransferase 3
- NAFLD
Nonalcoholic fatty liver disease
- PCa
Prostate cancer
- PPAT
Periprostatic adipose tissue
- PPI
Protein-protein interaction analysis
- PSA
Prostate-specific antigen
- PUFA
Polyunsaturated fatty acid
- QC
Quality control
- SLC44A2
Solute carrier family 44 member 2
- TAG
Triacylglycerol
- TAP
Transporter associated with antigen processing
- TAPBP
TAP binding protein
- TSS
Transcription start site
- TSS1500
1500 bp upstream of the transcription start site
- TSS200
200 bp upstream of the transcription start site
- UBC
Ubiquitin C
Background
Prostate cancer (PCa) is one of the most frequent malignancies in men and the second leading cause of cancer-related death in the North America and most western European countries [1, 2]. Epidemiological studies support obesity or excess adiposity as an important environmental risk factor for PCa, being primarily associated with advanced disease and death [3]. Periprostatic adipose tissue (PPAT), a white fat depot surrounding the prostate capsular-like structure, has been recognized to have the potential to exert pro-tumoral endocrine and paracrine influences on prostate cancer cell’s biological phenotypes [4]. There is now evidence that obesity and overweight result in excess fat deposit at PPAT [5], altered fatty acid profile [6], migration of tumor cells [7], secretion of a variety of adipokines, such as interleukin-1 beta (IL-1b), osteopontin, leptin, tumor necrosis factor alpha (TNF-a), and decreased adiponectin, thus contributing to a tumor microenvironment that ultimately facilitates PCa aggressiveness [7, 8].
DNA methylation is a well-known epigenetic mechanism resulting from the interaction between environmental factors and the genome [9]. DNA methylation with variation of CpG sites is associated with tissue-specific gene modulation and involved in phenotype transmission and in the development of diseases [10]. Excess adiposity, as a consequence of environmental factors such as excessive food consumption or inactive lifestyle, has been identified as a regulator of epigenetic modification in adipose tissue. Recent findings from experimental studies suggested that modification of DNA methylation pattern in adipose tissue and adipocytes was related with development of cancer, type 2 diabetes, and cardiovascular diseases through influencing metabolism and inflammation [11, 12, 13]. Additionally, several studies reported altered DNA methylation in PCa cells as compared with adjacent benign tissue, and some significantly methylated CpG sites and genes were found to be responsible for the occurrence and progression of PCa [14, 15, 16]. Nevertheless, the epigenome-wide DNA methylation profile of PPAT from excess adiposity PCa patients is currently unknown despite its potential mechanistic involvement in obesity association with PCa.
The aim of this study was to perform a epigenetic-wide association study (EWAS) in order to evaluate DNA methylation profile of PPAT obtained from obese/overweight (OB/OW) in comparison with normal weight (NW) PCa patients and identify differentially methylated sites. We also explored the consequential potential biological functions that account for the effect of PPAT from OB/OW subjects in PCa molecular mechanisms.
Methods
Study samples
This study included ten prostate cancer patients from the Portuguese Institute of Oncology, Porto Centre. Inclusion criteria and conditions of this study have been previously reported, including the procedures for PPAT collection, handling, and storage [4]. Briefly, PPAT was collected and immediately processed in the operating room and transported to the laboratory within 2 h in appropriate culture media and temperature conditions, in order to minimize pre-analytical errors. Patients’ signed informed consent and research procedures were approved by the institute’s ethics committee.
Clinicopathological characteristics of PCa patients by BMI category
Character | NW (n = 5) | OB/OW (n = 5) | P value |
---|---|---|---|
Age (years) | 65.2 ± 3.8 | 63.2 ± 2.5 | 0.67a |
BMI (kg/m2) | 23.0 ± 0.3 | 29.0 ± 0.9 | 0.0003a |
Gleason score | |||
< 7 | 2 (40%) | 1 (20%) | |
≥ 7 | 3 (60%) | 4 (80%) | 1.00b |
Stage | |||
OCPCa | 2 (40%) | 2 (40%) | |
EPCa | 3 (60%) | 3 (60%) | 1.00b |
Smoking status | |||
Yes | 1 | 5 | |
No | 4 | 0 | 0.05b |
PSA (ng/ml) | 10.7 ± 2.7 | 12.1 ± 3.23 | 0.74a |
Epigenome-wide DNA methylation analysis
DNA was isolated from PPAT using Puregene hisalt extraction method (Qiagen/Gentra). Briefly, the tissue was minced with scalpels in a sterile petri dish on ice and then transferred to Puregene Cell Kit for overnight Proteinase K digest at 55 °C. A second Proteinase K digest was done the next morning for 5 h. DNA from the digested tissue was purified using Puregene extraction protocol (Qiagen/Gentra). Purified DNA was washed 2× with 70% ethanol and DNA pellet air dried and rehydrated in TE (10 mM Tris-Cl, 1 mM EDTA pH 7.5). Epigenome-wide DNA methylation was analyzed using the Infinium Human Methylation450 (HM450) BeadChip (Illumina, San Diego, CA, USA) in the Center for Applied Genomics (Toronto). This array contains 485,577 probes, which cover 21,231 (99%) RefSeq genes. Briefly, DNA was bisulfite-converted using the EZ DNA methylation kit (Zymo Research, Orange, CA, USA) and then used on the Infinium Assay® followed by the Infinium HD Assay Methylation Protocol (Illumina). The imaging data on the BeadChips was captured by Illumina iScan system.
Data filtering and normalization
Raw methylation level for each probe was represented by methylation β value, which was calculated based on β = intensity of the methylated allele/(intensity of the unmethylated allele + intensity of the methylated allele + 100). M values were the logit transformation of β values based on M = log2 (β/(1 − β)), which makes the data more homoscedastic and appropriate for further bioinformatic and statistical analysis.
Methylation values were normalized using the functional normalization algorithm implemented in Minfi R package [18]. Quality control was performed by excluding CpG probes, which are found by Chen et al. to be cross-reactive with areas of the genome not at the site of interest [19], as well as control probes and probes on sex chromosomes. We analyzed a total of 438,458 CpG sites from the PPAT of 5 OB/OW PCa patients and 5 NW PCa patients.
Differential methylation analysis
A statistical linear modeling approach was applied to the detected differentially methylated CpG sites (DMCs) associated with obesity in PPAT using the Bioconductor “limma” package [20]. Hyper- or hypomethylation was determined when methylation levels of CpGs increased or decreased between the OB/OW PCa group and the NW PCa group based on mean different β > 0 or < 0. False discovery rate (FDR)-corrected P values were determined according to the method of Benjamin and Hochberg’s (BH method) multiple testing procedure [21].
Differentially methylated regions (DMRs) were identified using the “Bumphunter” method implemented in the “chAMP” R package with the parameters (B = 1000, useWeights = TRUE, minProbes = 10, pickCutoff = TRUE, and other settings with default values) [22].
The proportions of significant hyper- or hypomethylated CpGs were calculated and visualized according to their relation to the nearest genes or to the CpG islands, separately. Gene promoter region was defined as 1500 base pairs (bp) and 200 bp upstream of the transcription start site (TSS) (TSS1500 and TSS200) [23]. Identified genes were selected when more than two significantly hypermethylated CpGs were simultaneously located in the promoter region.
Functions, pathway, and network enrichment analysis
Gene ontology (GO) and KEGG pathway enrichment analyses were performed to explore the biological functions of significantly methylated genes using the online bioinformatic tool Enrichr [24]. Protein-protein interaction (PPI) analysis of all DMC-related genes was performed using NetworkAnalyst according to STRING database [25].
Association analysis between DNA methylation and gene expression
We have previously performed gene expression experiment of the PPAT of the 5 OB/OW PCa patients and the 5 NW PCa patients using the HG-U133 Plus 2.0 Affymetrix GeneChip Array (Affymetrix, Santa Clara, CA, USA) [4]. Differential gene expression (DGE) analysis between the OB/OW PCa patients and the NW PCa patients was re-performed using the Bioconductor “limma” package as previously described [4]. Spearman’s rank correlation analysis was performed between the methylation profiles of the hypermethylated CpGs and the gene expression profiles of the genes in PPAT.
Results
Clinical characteristics
Clinical characteristics of PCa patients in this study were stratified according to obesity classification groups and are presented in Table 1. Mean age, PSA level, Gleason sum score, and cancer stage in subjects with PCa were similar (P value > 0.05) between OB/OW and NW groups. As expected, the mean BMI of the OB/OW group was significantly higher than that of the NW subjects (P value < 0.01). All the patients in the OB/OW group are ex-smokers or active smokers, while only one patient in the NW group is a smoker (P value = 0.05).
Epigenome-wide DNA methylation profiling of PPAT
Differentially methylated CpG sites in PPAT between obese/overweight PCa patients and normal weight controls
Probe ID | Chromosome and coordinate (GRCh37) | Nearest gene | Relation to gene region | Relation to CpG island | DNAm β difference (%) | P value | Adjusted P value (< 0.25) |
---|---|---|---|---|---|---|---|
Hypermethylated CpG sites | |||||||
cg09476130 | chr1:159870086 | CCDC19 | TSS200 | Island | 12.1 | 1.87E−03 | 0.213 |
cg21293934 | chr18:14748230 | ANKRD30B | TSS200 | Island | 11.2 | 1.83E−03 | 0.212 |
cg16925210 | chr2:216946718 | PECR | TSS200 | Island | 11.2 | 2.44E−03 | 0.226 |
cg11625005 | chr5:1295737 | TERT | TSS1500 | Island | 11.1 | 1.38E−03 | 0.196 |
cg07039560 | chr5:140683681 | SLC25A2 | TSS200 | Island | 10.5 | 2.24E−03 | 0.222 |
cg00329447 | chr8:145028170 | PLEC1 | TSS200 | Island | 10.1 | 3.41E−03 | 0.244 |
cg24463471 | chr1:25257978 | RUNX3 | TSS1500 | Island | 9.9 | 3.58E−04 | 0.155 |
cg26149485 | chr19:2428350 | TIMM13 | TSS1500 | Island | 9.7 | 3.36E−04 | 0.154 |
cg05156901 | chr22:51016646 | CPT1B | TSS200 | Island | 9.3 | 3.05E−03 | 0.238 |
cg18689454 | chr21:45705694 | AIRE | TSS200 | Island | 9.3 | 7.33E−04 | 0.174 |
cg01454592 | chr3:49236800 | CCDC36 | TSS200 | Island | 9.3 | 2.89E−03 | 0.236 |
cg24041556 | chr19:10736059 | SLC44A2 | TSS200 | Island | 9.1 | 2.15E−05 | 0.110 |
cg22257574 | chr9:135754383 | C9orf98 | TSS200 | Island | 9.0 | 1.97E−05 | 0.110 |
cg23005885 | chr15:90543450 | ZNF710 | TSS1500 | Island | 8.9 | 6.20E−04 | 0.169 |
cg05726756 | chr17:46608288 | HOXB1 | TSS200 | Island | 8.6 | 1.67E−03 | 0.206 |
cg12782180 | chr7:127880932 | LEP | TSS1500 | Island | 8.5 | 9.78E−04 | 0.184 |
cg04675542 | chr5:150284416 | ZNF300 | TSS200 | Island | 8.4 | 3.42E−03 | 0.244 |
cg10134527 | chr6:33283015 | TAPBP | TSS1500 | Island | 8.4 | 3.19E−03 | 0.241 |
cg23387569 | chr12:58120011 | LOC100130776 | TSS200 | Island | 8.4 | 3.39E−04 | 0.155 |
cg17205324 | chr14:23835595 | EFS | TSS1500 | Island | 8.3 | 1.46E−04 | 0.133 |
cg24402300 | chr19:55591437 | EPS8L1 | TSS1500 | Island | 8.2 | 2.02E−03 | 0.216 |
cg18081258 | chr14:21494161 | NDRG2 | TSS1500 | Island | 8.2 | 1.05E−03 | 0.187 |
cg00730561 | chr10:102279703 | SEC31B | TSS200 | Island | 8.1 | 4.82E−04 | 0.162 |
cg17791651 | chr1:38513489 | POU3F1 | TSS1500 | Island | 8.0 | 1.31E−04 | 0.133 |
Hypomethylated CpG sites | |||||||
cg03462171 | chr16:1664488 | CRAMP1L | TSS200 | Island | − 8.2 | 2.30E−03 | 0.223 |
cg11648730 | chr5:92907151 | FLJ42709 | TSS1500 | Island | − 6.4 | 1.72E−03 | 0.207 |
cg04558166 | chr1:210001279 | C1orf107 | TSS200 | Island | − 4.0 | 1.75E−03 | 0.209 |
cg25472897 | chr8:145560555 | SCRT1 | TSS1500 | Island | − 3.1 | 1.53E−03 | 0.201 |
cg17612948 | chr5:110427863 | WDR36 | TSS200 | Island | − 3.0 | 1.70E−03 | 0.207 |
cg21665057 | chr3:196295764 | WDR53 | TSS1500 | Island | − 2.4 | 2.42E−03 | 0.225 |
cg12683173 | chr7:69063404 | AUTS2 | TSS1500 | Island | − 1.4 | 3.30E−03 | 0.242 |
cg04872557 | chr1:76190008 | ACADM | TSS200 | Island | − 1.0 | 1.96E−03 | 0.215 |
Epigenetic profiles of differentially methylated CpGs of PPAT between OB/OW and NW groups. Manhattan plots show epigenetic profiles of all increased methylated CpGs (a) and all decreased methylated CpGs (b). The X-axis shows chromosomes, and the Y-axis is a −log10 (P value). The black line represents the threshold of adjusted P value = 0.25. CpGs above the black line are significantly hyper- or hypomethylated. The volcano plot of DNA methylation (c) shows a significant difference in PPAT between the OB/OW and NW groups. Four thousand nine hundred eighty-five hypermethylated CpGs are labeled in red, and 541 hypomethylated CpGs are labeled in green (adjusted P value > 0.25). The proportions of hyper- and hypomethylated CpGs on each chromosome are shown in (d) and (e). The black line indicates if the proportions of hyper- and hypomethylated CpGs on a chromosome are higher than 6%. The distribution of significant DMCs (hyper- or hypomethylated CpGs) and globe DNA methylation CpGs in locations related to the nearest gene regions and CpG islands are shown in f and g. Hypermethylated CpGs are mainly located at TSS1500 (transcription start sites 1500), IGR (intergenic region), N-shore (the 2 kb regions upstream of the CpG island boundaries), and S-shore (the 2 kb regions downstream of the CpG island boundaries), and hypomethylated CpGs are mostly located at the gene body and open sea. The difference of the proportion of CpGs among the three CpG groups was calculated based on the χ2 test (*P < 0.05, **P < 0.01, ***P < 0.001, ns not significant). CpG islands were defined as DNA sequences (500 base windows; excluding most repetitive Alu-elements) with a GC base composition greater than 50% and a CpG observed/expected ratio of more than 0.6. The 2 kb regions immediately upstream (N_Shore) and downstream (S_Shore) of the CpG island boundaries were defined as “CpG island shores,” and the 2 kb regions upstream (N_Shelf) and downstream (S_Shelf) of the CpG island shores were referred as “CpG island shelves.” Open seas were the regions more than 4 kb from CpG islands
Chromosomal distribution of the DMCs
To further explore the methylation profile, we investigated the chromosome distribution of DMCs. Results showed that hypermethylated CpG sites were located at chromosomes 1, 6, 11, and 17 (proportion > 6%, Fig. 1d) and hypomethylated CpG sites were located at chromosomes 1, 2, 6, 7, and 11 (proportion > 6%, Fig. 1e).
Protein-protein interaction analysis. a A subnetwork composing of 247 nodes and 403 edges was generated using methylated genes. Blue dots represent the genes involved in prostate cancer; red and pink dots represent the seeds (methylated genes) according to the different P values; the gray dots represent the proteins which were closely interacted with the seeds, and the circle size represents the node degree. b The pathway enrichment analysis shows the subnetwork is mainly enriched in cancer pathways (P < 0.0001)
Functional enrichment analysis of significantly obesity-associated DMCs
To investigate the potential biological relevance of the significant DMCs, we further filtered 483 DMCs (distributed within 413 genes) from a total of 5526 DMCs according to their locations at both the gene promoter and CpG island (Additional file 6: Table S4). Four hundred seventy-five of the 483 DMCs (representing 404 genes) were hypermethylated. Functional enrichment analysis of the hypermethylated genes showed that these genes were enriched for biological processes, such as pattern specification process, neuron differentiation, neuron fate specification, and negative regulation of phosphate metabolic process (adjusted P value < 0.05, Additional file 7: Table S5), as well as molecular functions, such as neuropeptide receptor activity and sequence-specific DNA-binding RNA polymerase II transcription factor activity (adjusted P value < 0.1, Additional file 8: Table S6). KEGG pathway enrichment analysis showed that hypermethylated genes were involved in signaling pathways regulating pluripotency of stem cells, fatty acid metabolism, basal cell carcinoma, non-alcoholic fatty liver disease (NAFLD), and AMPK signaling pathway (P value < 0.05, Additional file 9: Table S7).
We mapped the 404 hypermethylated genes to the STRING database and generated a protein-protein interaction (PPI) network by the NetworkAnalyst. The largest subnetwork was identified to include 247 nodes (genes) and 403 edges (Fig. 2a). In the network, the size of the nodes was based on their degree values and the color of nodes was based on their P values. This network contained 118 seed genes from the DMCs, and the enrichment pathway analysis showed that the genes of the subnetwork were mostly involved in the pathways of prostate cancer and other cancers (Fig. 2b, Additional file 10: Table S8, adjusted P value < 0.05). Particularly, the gene UBC (ubiquitin C) was found to be a hub connecting with many other nodes in the network, suggesting that the gene may play important biological roles in the PPAT of obese PCa patients.
Selected genes with multiple methylated CpG sites
Visualization and analysis of hypermethylated CpG sites in TAPBP promoter.
a The chromosome positions of hypermethylated CpG sites show that DMCs are located at chr6 (p21.32), which is in the region of TSS1500 (pink square) of TAPBP and at the location of CpG island 31. b Methylation levels of eight CpG sites in PPAT from OB/OW and NW PCa patients have shown a significant difference. c Correlation analysis shows strong correlation (Spearman correlation coefficient r2 0.73~ 0.97) between the eight hypermethylated CpG sites based on the mean β value difference of individual probes
Differential methylated regions analysis
Differentially methylated regions (DMR) in PPAT of obese/overweight PCa patients compared to normal weight controls
DMR | Chr | Start–end (bp) | Size (bp) | P value | FDR | Located gene | DMCs* | Relation to CpG island |
---|---|---|---|---|---|---|---|---|
1 | 6 | 30,038,791–30,039,801 | 1010 | 5.11E−05 | 2.07E−02 | RNF39 | 37(0) | Island |
2 | 6 | 29,648,161–29,649,084 | 923 | 1.54E−03 | 2.08E−01 | ZFP57 # | 22(0) | Open sea |
3 | 17 | 71,228,123–71,228,832 | 709 | 3.10E−03 | 2.86E−01 | FAM104A | 4(3) | Island |
4 | 17 | 71,228,123–71,228,832 | 709 | 3.10E−03 | 2.86E−01 | C17orf80 | 10(8) | Island |
5 | 12 | 42,720,006–42,720,167 | 161 | 5.56E−03 | 2.86E−01 | PPHLN1 | 4(0) | Island |
6 | 6 | 31,650,735–31,651,158 | 423 | 5.59E−03 | 2.86E−01 | MIR4646 # | 16(0) | Island |
7 | 7 | 27,169,674–27,170,961 | 1287 | 5.66E−03 | 2.86E−01 | HOXA4 | 17(11) | Island |
8 | 6 | 33,282,736–33,283,145 | 409 | 5.87E−03 | 2.86E−01 | TAPBP | 18(18) | Island |
9 | 20 | 57,463,763–57,464,129 | 366 | 6.74E−03 | 2.86E−01 | GNAS | 15(0) | Island |
10 | 16 | 86,546,938–86,547,322 | 384 | 7.03E−03 | 2.86E−01 | FOXF1 | 4(0) | Shore |
Association analysis between DNA methylation and mRNA expression
Genes hypermethylated in promoters with significantly decreased gene expression
Gene symbol | DNA methylation | Gene expression | Correlation analysis | |||||
---|---|---|---|---|---|---|---|---|
Probe ID | DNAm β diff. (%) | Adjusted P value (< 0.25) | Probe ID | FC | P value (< 0.05) | Spearman’s rank correlation coefficient | P value (< 0.05) | |
UCN | cg20442078 | 5.6 | 0.17 | 8051061 | − 1.12 | 3.61E−02 | − 8.42E−01 | 2.23E−03 |
CCHCR1 | cg00160818 | 1.9 | 0.17 | 8124868 | − 1.14 | 1.73E−02 | − 7.45E−01 | 9.21E−03 |
CRB3 | cg14782015 | 4.3 | 0.20 | 8025041 | − 1.13 | 1.84E−02 | − 7.21E−01 | 1.21E−02 |
AGBL4 | cg21834207 | 3.2 | 0.13 | 7915971 | − 1.17 | 1.29E−02 | − 6.73E−01 | 1.97E−02 |
INSL3 | cg10174482 | 4.2 | 0.13 | 8035345 | − 1.13 | 4.94E−02 | − 6.36E−01 | 2.72E−02 |
ANKRD30B | cg21293934 | 11.2 | 0.21 | 8069499 | − 1.17 | 2.24E−02 | − 6.08E−01 | 3.11E−02 |
FADS1 | cg16213375 | 3.6 | 0.16 | 7948612 | − 1.8 | 9.55E−04 | − 5.88E−01 | 4.01E−02 |
PAPL | cg18481683 | 2.3 | 0.24 | 8028570 | − 1.19 | 1.45E−02 | − 5.52E−01 | 5.21E−02 |
MOGAT1 | cg12678667 | 4 | 0.15 | 8048725 | − 1.28 | 3.87E−02 | − 4.67E−01 | 8.91E−02 |
PPP1R1B | cg09762778 | 5 | 0.12 | 8006865 | − 1.27 | 4.74E−02 | − 4.67E−01 | 8.91E−02 |
PRUNE2 | cg00390775 | 4.4 | 0.15 | 8161884 | − 1.31 | 1.91E−02 | − 3.82E−01 | 1.39E−01 |
CIDEA | cg18309817 | 1.8 | 0.18 | 8020211 | − 1.32 | 3.21E−02 | − 2.97E−01 | 2.03E−01 |
PCYT2 | cg19583655 | 6.2 | 0.21 | 8019280 | − 1.26 | 6.38E−04 | − 1.88E−01 | 3.04E−01 |
SCUBE1 | cg07697597 | 1.7 | 0.23 | 8076586 | − 1.23 | 9.03E−03 | − 4.24E−02 | 4.59E−01 |
Discussion
This pilot study revealed significant differences of DNA methylation profiles between the PPATs from OB/OW versus NW PCa patients. Variations in global DNA methylation demonstrated that excess adiposity played an important role in DNA methylation level of PPAT tissues in prostate cancer patients, which provide an opportunity to explore the effect of obesity on PPAT epigenetic modification and subsequently on prostate cancer. These findings reported for the first time in PPAT depot are in concordance with previous works reporting that excess adiposity and BMI activate DNA methylation in adipose tissue [27, 28, 29]. Thus, considering the present understanding of the potential causal relationship between excess adiposity and cancer [30], diabetes [11], and cardiovascular disease [31], our results provide methylated candidate genes, which might foster research on the potential biological mechanisms underlying epigenetic regulation of PPAT by excess adiposity and prostate cancer.
Given that DNA methylation of CpGs located at promoters and islands are associated with gene transcription silencing, we performed a strict filtering of DMCs and explored the biological functions of all promoter hypermethylated genes, aiming to find the critical methylated CpGs in the PPAT between the obese and normal weight PCa patients. Bioinformatic analysis showed that the enriched pathways were mostly involved in metabolic disorders, particularly fatty acid degradation and glycerolipid and choline metabolism. These pathways are known to mediate the pro-tumoral effect of white adipose tissue in tumors, thus contributing to tumorigenesis and metastasis [32, 33], particularly in prostate cancer [5]. Findings from other oncological models highlight excess adiposity-associated impact in methylation markers known to associate with potential effect in the cancer microenvironment (e.g., aromatase, prostaglandin E2 receptor in breast cancer) [34, 35]. Obesity has also been shown to associate with methylation of cancer-related genes (E-cadherin, p16, and RAR-β(2)) directly in malignant breast cells [36, 37].
Proposed mechanisms with differentially methylated genes from PPAT of OB/OW prostate cancer patients. Hypermethylated genes in periprostatic adipose tissue of patients with increased adiposity might contribute towards the modulation of prostate tumor microenvironment. The genes that might be related to tumor microenvironment include choline transporter-like protein 2 (CTL2, which was a rate-limiting step of choline metabolism by transporting extracellular choline into cell and mitochondria), carnitine palmitoyltransferase 1B and 1C (CPT1B and CPT1C, which encode the rate-limiting enzymes of long-chain fatty acid β-oxidation by controlling transportation of long-chain fatty acyl-CoAs from cytoplasm across outer mitochondria membrane), medium-chain-specific acyl-CoA dehydrogenase (ACADM, which catalyzes the initial step of medium-chain fatty acid β-oxidation in mitochondria), fatty acid desaturase 1 (FADS1, which was correlated with fatty acid metabolism by catalyzing polyunsaturated fatty acid biosynthesis), monoacylglycerol O-acyltransferases 1 and 3 (MOGAT1 and MOGAT3, which catalyze the formation of diacylglycerol by transferring fatty acyl-CoA to 2-monoacylglycerol), which contributes to metabolic disorder in adipose tissue by regulating the metabolism of lipid, choline, and glycerolipid. Another gene with hypermethylated promoter, TAPBP (transporter associated with antigen processing (TAP) transport protein), could influence tumor supervision of immune cells in PPAT by altering tumor antigen presentation process from TAP to MHC class I in endoplasmic reticulum and result in tumor metastasis and cancer progression. The black downward arrows represent the promoter hypermethylated genes (in blue containers), and the red arrows represent the possible consequence of these methylated genes. LCFA long-chain fatty acid, MCFA media-chain fatty acid, PUFA polyunsaturated fatty acid, HUFA high unsaturated fatty acid, MAG monoacylglycerol, DAG dionoacylglycerol
LCFA and ACADM genes (aliases MCAD, medium-chain acyl-CoA dehydrogenase) coding for metabolic enzymes presented increased methylation in the PPAT of the OB/OW group. ACADM is the critical enzyme of the initial step of β-oxidation and controls the medium-chain fatty acid (MCFA) metabolism by catalyzing the dehydrogenation of medium-chain Acyl-CoA, which is the common middle product of MCFA and LCFA, in the mitochondria. Mutations in ACADM cause MCAD deficiency, which resulted in fatty acid oxidation disorder leading to disease or infantile death [42, 43, 44]. Greco et al. [45] reported inverse association between ACADM transcript abundance with fat content in the human liver. Our findings suggest that the hypermethylated ACADM found in the PPAT of OB/OW PCa patients might fail to generate medium-chain acyl-CoA β-oxidation and result in MCFA and LCFA accumulation in adipose tissue, providing a favorable tumor microenvironment for PCa cell aggressiveness (Fig. 4). Additional functional studies are required to confirm this assumption.
The hypermethylation of the FADS1 (fatty acid desaturase 1) promoter, whose transcriptional activity was significantly decreased in OB/OW PCa patients in agreement with our previous study [4], has been described as correlated with polyunsaturated fatty acid (PUFA) metabolism by catalyzing the biosynthesis of highly unsaturated fatty acids (HUFA) from the catalysis of dihomo-gamma-linoleic acid (DGLA, 20:3 n-6) and eicosatetraenoic acid (ETA, 20:4 n-3) desaturation, in order to generate arachidonic acid (AA, 20:4 n-6) and eicosapentaenoic acid (EPA, 20:5 n-3) [46]. Genetic variants in the FADS1 and FADS2 gene clusters have been associated with altered (n-6) and (n-3) PUFA metabolism [47, 48], whereas metabolic disorder in PUFA exerted effects on PCa by mediating the formation of eicosanoid inflammatory mediators (prostaglandins, leukotrienes, thromboxanes, and lipoxins), angiogenesis, immune cell regulation, and membrane structure and function [49, 50]. These results illustrated that the epigenetic modifications of FADS1 may play important roles in the regulation of fatty acid metabolic genes on PPAT in response to excess adiposity (Fig. 4).
Besides abnormal fatty acid metabolism, DMC-related genes identified in our study were also correlated with glycerolipid metabolism. MOGAT1 and MOGAT3 encode the monoacylglycerol O-acyltransferase (MOGAT) and catalyze the formation of diacylglycerol (DAG) from monoacylglycerol (MAG), which is the precursor of phosphatidylcholine, phosphatidylethanolamine, and triacylglycerol (TAG), by transferring fatty acyl-CoA to 2-monoacylglycerol [51]. While human MGAT1 (aliases for MOGAT1) is involved in intestinal dietary fat absorption and TAG synthesis in the liver, its function in adipose tissue has yet to be elucidated. The expression of MGAT1 was increased in the liver of diet-induced obese mice with nonalcoholic fatty liver disease (NAFLD), but, interestingly, there was increased DAG accumulation and no inflammatory injury reduction in hepatocytes after MGAT1 knockdown. Similarly, MOGAT3 was mostly expressed in the human intestine and liver and maintained a significant DGAT (diacylglycerol O-acyltransferase) activity. Although results indicate that the metabolic mechanism of lipid regulation by MGAT1 and MOGAT3 was altered, evidence of association between lipid metabolic disorders caused by aberrant expression of MGAT1/MOGAT3 and PCa are lacking. Our data indicate the methylation of MOGAT1 and 3 genes in PPAT may play important roles in response to excess adiposity by modulating glycerolipid metabolism (Fig. 4).
Choline metabolic disorder might be caused by epigenetic regulation of SLC44A2 (solute carrier family 44 member 2), which encodes choline transporter-like protein 2 (CTL2) and is mainly expressed on blood plasma and mitochondrial membrane of different organisms and cell types. This transporter is a rate-limiting step in choline metabolism by transporting extracellular choline into cell and mitochondria. Choline is essential for synthesizing membrane phospholipid and neurotransmitter acetylcholine and used as a donor of methyl groups via choline oxidized in mitochondria [52]. The choline transporter has been associated with choline metabolic disorders, thus playing an important role in regulating immune response, inflammation, and oxidation [53, 54]. Concordantly, abnormal choline metabolism emerged as a metabolic hallmark, associated with oncogenesis and tumor progression in prostate cancer and other malignancies [55, 56, 57]. The increased uptake of choline by the cancer cell was important to meet the needs of phosphatidylcholine synthesis [58]. We hypothesize that hypermethylated SLC44A2 in adipocytes might be associated with lower uptake and oxidation of extracellular choline, resulting in choline accumulation in PPAT extracellular media (Fig. 4) and increasing the availability of choline for PCa cell metabolism.
Besides metabolic modifications, altered immune regulation pathways were also enriched in DMC-related genes. TAPBP (alias tapasin) encodes a transmembrane glycoprotein, which mediates the interaction between MHC class I molecules and a transport protein TAP (transporter associated with antigen processing), being responsible for antigen processing and presentation. This mechanism occurs via mediating TAP to translocate endo/exogenous antigen peptides from the cytoplasm into the endoplasmic reticulum and deliver the antigen peptides to MHC class I molecules. The cancer cell’s survival depends on successful escape to immune surveillance. Loss of MHC class I has been described as a major immune evasion strategy for cancer cells. Downregulation of antigen-presenting MHC class I pathway in tumor cells was a common mechanism for tumor cells escaped from specific immune responses, which can be associated with coordinated silencing of antigen-presenting machinery genes, such as TAPBP [59]. Cross-presentation is the ability of certain antigen-presenting cells to take up, process, and present extracellular antigens with MHC class I molecules to CD8+ T cells. This process is necessary for immunity against most tumors. Recent studies revealed that TAPBP is a major target for cancer immune evasion mechanisms and decreased TAPBP expression in cancer was associated with reduced CD8+ T cell-mediated killing of the tumor cells, lowered immune responses, and enhanced tumor metastases via downregulation of antigen presentation the MHC class I pathway [60, 61]. Our results showed that TAPBP promoter hypermethylation in the PPAT of obese PCa subjects likely reduced the expression or activity of TAPBP, downregulating tumor cell’s antigen presentation of immune cells in PPAT, leading to impaired CD8+ T cell activation (Fig. 4). This indicates that methylation of TAPBP might be a mechanism by which prostate cancer cells escape the immune surveillance and provide an appropriate microenvironment for tumor aggressiveness, allowing prostatic cancer cells’ transfer, spread, and growth. The significant DMR identified with eight DMCs located in the TAPBP promoter further supported its role in prostate cancer.
From the PPI analysis, the network which was connected through ubiquitin C is characterized, suggesting UBC played a significant biological function with the methylated genes in PPAT between OB/OW and NW patients and somehow was correlated with the methylation. Ubiquitin is much known with the functions including roles in protein degradation, DNA repair, cell cycle regulation, kinase modification, and cell signaling pathways [62]. Recent reports expressed that the ubiquitin-proteasome system was associated with the progression and metastasis of prostate cancers [63, 64]. And long-term silencing of the UBC was found to be correlated with DNA methylation at the promoters [65]. Additional studies are needed to clarify whether the protein network for methylated genes impacts prostate cancer and if this difference is associated with ubiquitin C.
Although we present the first report on periprostatic adipose tissue methylation profile in association with excess adiposity measured by BMI, our results should be interpreted in the context of several potential limitations. This study is limited by small sample size, even though representative groups of OB/OW and NW are likely to be selected following the strict inclusion/exclusion criteria and between-group match by clinicopathological and demographic variables. Although we matched patients by clinicopathological characteristics between adiposity groups, tobacco smoking was more frequent among OB/OW compared with NW patients. Actually, albeit we cannot exclude an effect of smoking status on the presumably adiposity-associated findings presented herein, due to a known effect of tobacco on overall DNA methylation, data from previous reports indicate that methylation profiles are tissue-specific [66, 67] and that adiposity-associated DNA methylation occurs independently of tobacco smoking [68, 69]. Future studies will benefit from the confirmation of these results in larger sample sizes, determination of correspondence to matched prostate tumor methylation patterns, investigation of interactome at the interface between tumor and PPAT, and prospective investigations on the value of PPAT epigenetic modifications on cancer recurrence and survival. Future validation and replication are important to establish the accuracy and generalizability of the reported associations.
In summary, we observed differences in PPAT methylation between NW and OB individuals at several loci known to be involved in the metabolism of choline (SLC44A2), fatty acids (CPT1B, CPT1C, ACADM, FADS1), and glycerolipid (MOGAT1, MOGAT3) and in the regulation of exogenous tumor antigen presentation (TAPBP). These findings suggest a relationship of adiposity status with the methylation profile, which ultimately modulates tumor microenvironment and may influence PCa behavior.
Conclusions
In this preliminary study, we report DNA methylation changes in PPAT underlying the association between excess adiposity and PCa. Whole epigenome methylation profiling of PPAT of PCa patients revealed significant differences in OB/OW versus normal weight subjects. Epigenetic imprinting in association with excess adiposity expressed the methylated modifications in genes functionally related with lipid metabolism and immune function, which could ultimately contribute to an unfavorable tumor microenvironment and decreased immune surveillance for prostate tumors. This association analyses provided us novel insights into how prostate cancer patients with excess adiposity differ from those of patients with normal weight in epigenome. Findings from this study warrant confirmation in PPAT samples from larger number of patients.
Notes
Funding
This work was supported in part by the Natural Sciences and Engineering Research Council of Canada, Manitoba Research Health Council, University of Manitoba, and China Scholarship Council.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors’ contributions
YC designed and implemented the experiments and drafted the manuscripts. JY helped generate the figures. PH supervised and monitored the whole project. RR, CM, AM, CP, VC, JGA, GF, and AR performed the tissue handling and processing, isolated the RNA, and conducted the gene expression experiment. RR, AF, AM, and CM collected the adipose tissue and clinicopathological patient information and edited the manuscript. RR, GF, and PH designed the study and edited the manuscript. All authors read, edited, and approved the final manuscript.
Ethics approval and consent to participate
Informed consent has been obtained from all the participants, and the study has received ethical approval from the Ethics Committee of Portuguese Institute of Oncology, Porto Centre.
Consent for publication
The participants/patients have given their consent for their data to be published in the report.
Competing interests
The authors declare that they have no competing interests.
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary material
References
- 1.Labbé DP, Zadra G, Ebot EM, Mucci LA, Kantoff PW, Loda M, Brown M. Role of diet in prostate cancer: the epigenetic link. Oncogene. 2014;34(36):4683–91.CrossRefPubMedPubMedCentralGoogle Scholar
- 2.Sutcliffe S, Colditz GA. Prostate cancer: is it time to expand the research focus to early-life exposures? Nat Rev Cancer. 2013;13:208–18.CrossRefPubMedPubMedCentralGoogle Scholar
- 3.Peisch SF, Van Blarigan EL, Chan JM, Stampfer MJ, Kenfield SA. Prostate cancer progression and mortality: a review of diet and lifestyle factors. World J Urol. 2017;35(6):867–74.CrossRefPubMedGoogle Scholar
- 4.Ribeiro R, Monteiro C, Catalán V, Hu P, Cunha V, Rodríguez A, Gómez-Ambrosi J, Fraga A, Príncipe P, Lobato C, Lobo F, Morais A, Silva V, Sanches-Magalhães J, Oliveira J, Pina F, Lopes C, Medeiros R, Frühbeck G. Obesity and prostate cancer: gene expression signature of human periprostatic adipose tissue. BMC Med. 2012;10:108.CrossRefPubMedPubMedCentralGoogle Scholar
- 5.Venkatasubramanian PN, Brendler CB, Plunkett BA, Crawford SE, Fitchev PS, Morgan G, Cornwell ML, McGuire MS, Wyrwicz AM, Doll JA. Periprostatic adipose tissue from obese prostate cancer patients promotes tumor and endothelial cell proliferation: a functional and MR imaging pilot study. Prostate. 2014;74:326–35.CrossRefPubMedGoogle Scholar
- 6.Iordanescu G, Brendler C, Crawford SE, Wyrwicz AM, Venkatasubramanian PN, Doll JA. MRS measured fatty acid composition of periprostatic adipose tissue correlates with pathological measures of prostate cancer aggressiveness. J Magn Reson Imaging. 2015;42(3):651–7.CrossRefPubMedGoogle Scholar
- 7.Laurent V, Guérard A, Mazerolles C, Le Gonidec S, Toulet A, Nieto L, Zaidi F, Majed B, Garandeau D, Socrier Y, Golzio M, Cadoudal T, Chaoui K, Dray C, Monsarrat B, Schiltz O, Wang YY, Couderc B, Valet P, Malavaud B, Muller C. Periprostatic adipocytes act as a driving force for prostate cancer progression in obesity. Nat Commun. 2016;7:10230.CrossRefPubMedPubMedCentralGoogle Scholar
- 8.Ribeiro R, Monteiro C, Cunha V, Oliveira MJ, Freitas M, Fraga A, Príncipe P, Lobato C, Lobo F, Morais A, Silva V, Sanches-Magalhães J, Oliveira J, Pina F, Mota-Pinto A, Lopes C, Medeiros R. Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro. J Exp Clin Cancer Res. 2012;31:32.CrossRefPubMedPubMedCentralGoogle Scholar
- 9.Liu L, Li Y, Tollefsbol TO. Gene-environment interactions and epigenetic basis of human diseases. Curr Issues Mol Biol. 2008;10(2):25–36.PubMedPubMedCentralGoogle Scholar
- 10.Tost J. DNA methylation: an introduction to the biology and the disease-associated changes of a promising biomarker. Methods Mol Biol. 2009;507:3–20.CrossRefPubMedGoogle Scholar
- 11.Barres R, Zierath J. DNA methylation in metabolic disorders. Am J Clin Nutr. 2011;93:897S–00.CrossRefPubMedGoogle Scholar
- 12.Pinnick KE, Karpe F. DNA methylation of genes in adipose tissue. Proc Nutr Soc. 2011;70(1):57–63.CrossRefPubMedGoogle Scholar
- 13.Benton MC, Johnstone A, Eccles D, Harmon B, Hayes MT, Lea RA, Griffiths L, Hoffman EP, Stubbs RS, Macartney-Coxson D. An analysis of DNA methylation in human adipose tissue reveals differential modification of obesity genes before and after gastric bypass and weight loss. Genome Biol. 2015;16:8.CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Wu Y, Davison J, Qu X, Morrissey C, Storer B, Brown L, Vessella R, Nelson P, Fang M. Methylation profiling identified novel differentially methylated markers including OPCML and FLRT2 in prostate cancer. Epigenetics. 2016;11(4):247–58.CrossRefPubMedPubMedCentralGoogle Scholar
- 15.Shui IM, Wong CJ, Zhao S, Kolb S, Ebot EM, Geybels MS, Rubicz R, Wright JL, Lin DW, Klotzle B, Bibikova M, Fan JB, Ostrander EA, Feng Z, Stanford JL. Prostate tumor DNA methylation is associated with cigarette smoking and adverse prostate cancer outcomes. Cancer. 2016;122(14):2168–77.CrossRefPubMedPubMedCentralGoogle Scholar
- 16.Kobayashi Y, Absher D. DNA methylation profiling reveals novel biomarkers and important roles for DNA methyltransferases in prostate cancer. Genome Res. 2011;21(7):1017–27.CrossRefPubMedPubMedCentralGoogle Scholar
- 17.Fraga A, Ribeiro R, Vizcaíno JR, Coutinho H, Lopes JM, Príncipe P, Lobato C, Lopes C, Medeiros R. Genetic polymorphisms in key hypoxia-regulated downstream molecules and phenotypic correlation in prostate cancer. BMC Urol. 2017;17:12.CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Aryee MJ, Jaffe AE, Corrada-Bravo H, Ladd-Acosta C, Feinberg AP, Hansen KD, Irizarry RA. Minfi: a flexible and comprehensive Bioconductor package for the analysis of Infinium DNA methylation microarrays. Bioinformatics. 2014;30(10):1363–9.CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Chen YA, Lemire M, Choufani S, Butcher DT, Grafodatskaya D, Zanke BW, Gallinger S, Hudson TJ, Weksberg R. Discovery of cross-reactive probes and polymorphic CpGs in the Illumina Infinium HumanMethylation450 microarray. Epigenetics. 2013; 11:8(2).Google Scholar
- 20.Ritchie ME, Phipson B, Wu D, Hu Y, Law CW, Shi W, Smyth GK. Limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 2015;43(7):289–300.CrossRefGoogle Scholar
- 21.Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc B. 1995;57(1):289–300.Google Scholar
- 22.Morris TJ, Butcher LM, Feber A, Teschendorff AE, Chakravarthy AR, Wojdacz TK, Beck S. ChAMP: 450k chip analysis methylation pipeline. Bioinformatics. 2014;30(3):428–30.CrossRefPubMedGoogle Scholar
- 23.Wang D, Yan L, Hu Q, Sucheston LE, Higgins MJ, Ambrosone CB, Johnson CS, Smiraglia DJ, Liu S. IMA: an R package for high-throughput analysis of Illumina’s 450K Infinium methylation data. Bioinformatics. 2012;28(5):729–30.CrossRefPubMedPubMedCentralGoogle Scholar
- 24.Chen EY, Tan CM, Kou Y, Duan Q, Wang Z, Meirelles GV, Clark NR, Ma'ayan A. Enrichr: interactive and collaborative HTML5 gene list enrichment analysis tool. BMC Bioinformatics. 2013;14:128.CrossRefPubMedPubMedCentralGoogle Scholar
- 25.Xia J, Gill EE, Hancock REW. NetworkAnalyst for statistical, visual and network-based meta-analysis of gene expression data. Nat Protoc. 2015;10(6):823–44.CrossRefPubMedGoogle Scholar
- 26.Jones PA, Takai D. The role of DNA methylation in mammalian epigenetics. Science. 2001;293(5532):1068–70.CrossRefPubMedGoogle Scholar
- 27.Dick KJ, Nelson CP, Tsaprouni L, Sandling JK, Aïssi D, Wahl S, Meduri E, Morange PE, Gagnon F, Grallert H, Waldenberger M, Peters A, Erdmann J, Hengstenberg C, Cambien F, Goodall AH, Ouwehand WH, Schunkert H, Thompson JR, Spector TD, Gieger C, Trégouët DA, Deloukas P, Samani NJ. DNA methylation and body-mass index: a genome-wide analysis. Lancet. 2014;383(9933):1990–8.CrossRefPubMedGoogle Scholar
- 28.Rönn T, Volkov P, Gillberg L, Kokosar M, Perfilyev A, Jacobsen AL, Jørgensen SW, Brøns C, Jansson PA, Eriksson KF, Pedersen O, Hansen T, Groop L, Stener-Victorin E, Vaag A, Nilsson E, Ling C. Impact of age, BMI and HbA1c levels on the genome-wide DNA methylation and mRNA expression patterns in human adipose tissue and identification of epigenetic biomarkers in blood. Hum Mol Genet. 2015;24(13):3792–813.PubMedGoogle Scholar
- 29.Zhang B, Zhou Y, Lin N, Lowdon RF, Hong C, Nagarajan RP, Cheng JB, Li D, Stevens M, Lee HJ, Xing X, Zhou J, Sundaram V, Elliott G, Gu J, Shi T, Gascard P, Sigaroudinia M, Tlsty TD, Kadlecek T, Weiss A, O'Geen H, Farnham PJ, Maire CL, Ligon KL, Madden PA, Tam A, Moore R, Hirst M, Marra MA, Zhang B, Costello JF, Wang T. Functional DNA methylation differences between tissues, cell types, and across individuals discovered using the M&M algorithm. Genome Res. 2013;23(9):1522–40.CrossRefPubMedPubMedCentralGoogle Scholar
- 30.Dedeurwaerder S, Desmedt C, Calonne E, Singhal SK, Haibe-Kains B, Defrance M, Michiels S, Volkmar M, Deplus R, Luciani J, Lallemand F, Larsimont D, Toussaint J, Haussy S, Rothé F, Rouas G, Metzger O, Majjaj S, Saini K, Putmans P, Hames G, van Baren N, Coulie PG, Piccart M, Sotiriou C, Fuks F. DNA methylation profiling reveals a predominant immune component in breast cancers. EMBO Mol Med. 2011;3(12):726–41.CrossRefPubMedPubMedCentralGoogle Scholar
- 31.Wang X, Zhu H, Snieder H, Su S, Munn D, Harshfield G, Maria BL, Dong Y, Treiber F, Gutin B, Shi H. Obesity related methylation changes in DNA of peripheral blood leukocytes. BMC Med. 2010;8:87.CrossRefPubMedPubMedCentralGoogle Scholar
- 32.Booth A, Magnuson A, Fouts J, Foster M. Adipose tissue, obesity and adipokines: role in cancer promotion. Horm Mol Biol Clin Investig. 2015;21(1):57–74.PubMedGoogle Scholar
- 33.Hefetz-Sela S, Scherer PE. Adipocytes: impact on tumor growth and potential sites for therapeutic intervention. Pharmacol Ther. 2013;138(2):197–210.CrossRefPubMedPubMedCentralGoogle Scholar
- 34.Zeng XC, Ao X, Yang HF, Zhang GX, Li WH, Liu QL, Tang YL, Xie YC, He WG, Huang YN, Zhang L, Li RJ. Differential expression of stromal aromatase in obese females is regulated by DNA methylation. Mol Med Rep. 2014;10(2):887–90.CrossRefPubMedGoogle Scholar
- 35.To SQ, Takagi K, Miki Y, Suzuki K, Abe E, Yang Y, Sasano H, Simpson ER, Knower KC, Clyne CD. Epigenetic mechanisms regulate the prostaglandin E receptor 2 in breast cancer. J Steroid Biochem Mol Biol. 2012;132:331–8.CrossRefPubMedGoogle Scholar
- 36.Tao MH, Marian C, Nie J, Ambrosone C, Krishnan SS, Edge SB, Trevisan M, Shields PG, Freudenheim JL. Body mass and DNA promoter methylation in breast tumors in the Western New York Exposures and Breast Cancer Study. Am J Clin Nutr. 2011;94(3):831–8.CrossRefPubMedPubMedCentralGoogle Scholar
- 37.Zolochevska O, Shearer J, Ellis J, Fokina V, Shah F, Gimble JM, Figueiredo ML. Human adipose-derived mesenchymal stromal cell pigment epithelium-derived factor cytotherapy modifies genetic and epigenetic profiles of prostate cancer cells. Cytotherapy. 2014;16(3):346–56.CrossRefPubMedPubMedCentralGoogle Scholar
- 38.Serra D, Mera P, Malandrino MI, Mir JF, Herrero L. Mitochondrial fatty acid oxidation in obesity. Antioxid Redox Signal. 2013;19(3):269–84.CrossRefPubMedPubMedCentralGoogle Scholar
- 39.Maples JM, Brault JJ, Witczak CA, Park S, Hubal MJ, Weber TM, Houmard JA, Shewchuk BM. Differential epigenetic and transcriptional response of the skeletal muscle carnitine palmitoyltransferase 1B (CPT1B) gene to lipid exposure with obesity. Am J Physiol Endocrinol Metab. 2015;309(4):E345–56.CrossRefPubMedPubMedCentralGoogle Scholar
- 40.Wolfgang MJ, Kurama T, Dai Y, Suwa A, Asaumi M, Matsumoto S, Cha SH, Shimokawa T, Lane MD. The brain-specific carnitine palmitoyltransferase-1c regulates energy homeostasis. Proc Natl Acad Sci U S A. 2006;103(19):7282–7.CrossRefPubMedPubMedCentralGoogle Scholar
- 41.van Tienen FHJ, van der Kallen CJH, Lindsey PJ, Wanders RJ, van Greevenbroek MM, Smeets HJM. Preadipocytes of type 2 diabetes subjects display an intrinsic gene expression profile of decreased differentiation capacity. Int J Obes. 2011;35(9):1154–64.CrossRefGoogle Scholar
- 42.Hara K, Tajima G, Okada S, Tsumura M, Kagawa R, Shirao K, Ohno Y, Yasunaga S, Ohtsubo M, Hata I, Sakura N, Shigematsu Y, Takihara Y, Kobayashi M. Significance of ACADM mutations identified through newborn screening of MCAD deficiency in Japan. Mol Genet Metab. 2016;118(1):9–14.CrossRefPubMedGoogle Scholar
- 43.Grünert SC, Wehrle A, Villavicencio-Lorini P, Lausch E, Vetter B, Schwab KO, Tucci S, Spiekerkoetter U. Medium-chain acyl-CoA dehydrogenase deficiency associated with a novel splice mutation in the ACADM gene missed by newborn screening. BMC Med Genet. 2015;16(1):56–65.CrossRefPubMedPubMedCentralGoogle Scholar
- 44.Ji S, Yang R, Lu C, Qiu Z, Yan C, Zhao Z. Differential expression of PPARγ, FASN, and ACADM genes in various adipose tissues and longissimus dorsi muscle from Yanbian yellow cattle and Yan yellow cattle. Asian-Australasian J Anim Sci. 2014;27(1):10–8.CrossRefGoogle Scholar
- 45.Greco D, Kotronen A, Westerbacka J, et al. Gene expression in human NAFLD. 2008:1281–1287. doi: https://doi.org/10.1152/ajpgi.00074.2008.
- 46.Berquin IM, Edwards IJ, Kridel SJ, Chen YQ. Polyunsaturated fatty acid metabolism in prostate cancer. Cancer Metastasis Rev. 2011;30(3–4):295–309.CrossRefPubMedGoogle Scholar
- 47.Lattka E, Illig T, Koletzko B, Heinrich J. Genetic variants of the FADS1 FADS2 gene cluster as related to essential fatty acid metabolism. Curr Opin Lipidol. 2010;21(1):64–9.CrossRefPubMedGoogle Scholar
- 48.Glaser C, Heinrich J, Koletzko B. Role of FADS1 and FADS2 polymorphisms in polyunsaturated fatty acid metabolism. Metabolism. 2010;59(7):993–9.CrossRefPubMedGoogle Scholar
- 49.Suburu J, Chen YQ. Lipids and prostate cancer. Prostaglandins Other Lipid Mediat. 2012;98(1–2):1–10.CrossRefPubMedPubMedCentralGoogle Scholar
- 50.Chen YQ, Edwards IJ, Kridel SJ, Thornburg T, Berquin IM. Dietary fat–gene interactions in cancer. Cancer Metastasis Reviews. 2007;26(3–4):535–51.Google Scholar
- 51.Schweitzer GG, Finck BN. Targeting hepatic glycerolipid synthesis and turnover to treat fatty liver disease. Advances in Hepatology. 2014;2014:498369.CrossRefGoogle Scholar
- 52.Glunde K, Bhujwalla ZM, Ronen SM. Choline metabolism in malignant transformation. Nat Rev Cancer. 2011;11(12):835–48.CrossRefPubMedPubMedCentralGoogle Scholar
- 53.Snider S. Choline transport links phospholipid metabolism and inflammation in macrophages. 2017. http://hdl.handle.net/10393/35715. Accessed 28 July 2017.Google Scholar
- 54.Traiffort E, O’Regan S, Ruat M. The choline transporter-like family SLC44: properties and roles in human diseases. Mol Asp Med. 2013;34(2–3):646–54.CrossRefGoogle Scholar
- 55.Kouji H, Inazu M, Yamada T, Tajima H, Aoki T, Matsumiya T. Molecular and functional characterization of choline transporter in human colon carcinoma HT-29 cells. Arch Biochem Biophys. 2009;483(1):90–8.CrossRefPubMedGoogle Scholar
- 56.Nishiyama R, Nagashima F, Iwao B, Kawai Y, Inoue K, Midori A, Yamanaka T, Uchino H, Inazu M. Identification and functional analysis of choline transporter in tongue cancer: a novel molecular target for tongue cancer therapy. J Pharmacol Sci. 2016;131(2):101–9.CrossRefPubMedGoogle Scholar
- 57.Mattie M, Raitano A, Morrison K, Morrison K, An Z, Capo L, Verlinsky A, Leavitt M, Ou J, Nadell R, Aviña H, Guevara C, Malik F, Moser R, Duniho S, Coleman J, Li Y, Pereira DS, Doñate F, Joseph IB, Challita-Eid P, Benjamin D, Stover DR. The discovery and preclinical development of ASG-5ME, an antibody-drug conjugate targeting SLC44A4-positive epithelial tumors including pancreatic and prostate cancer. Mol Cancer Ther. 2016;15(11):2679–87.CrossRefPubMedGoogle Scholar
- 58.Sutinen E, Nurmi M, Roivainen A, Varpula M, Tolvanen T, Lehikoinen P, Minn H. Kinetics of [11C]choline uptake in prostate cancer: a PET study. Eur J Nucl Med Mol Imaging. 2004;31(3):317–24.CrossRefPubMedGoogle Scholar
- 59.Vlková V, Štěpánek I, Hrušková V, Šenigl F, Mayerová V, Šrámek M, Šímová J, Bieblová J, Indrová M, Hejhal T, Dérian N, Klatzmann D, Six A, Reiniš M. Epigenetic regulations in the IFNγ signalling pathway: IFNγ-mediated MHC class I upregulation on tumour cells is associated with DNA demethylation of antigen-presenting machinery genes. Oncotarget. 2014;5(16):6923–35.CrossRefPubMedPubMedCentralGoogle Scholar
- 60.Pedersen MH, Hood BL, Beck HC, Conrads TP, Ditzel HJ, Leth-Larsen R. Downregulation of antigen presentation-associated pathway proteins is linked to poor outcome in triple-negative breast cancer patient tumors. Oncoimmunology. 2017;6(5):e1305531.CrossRefPubMedPubMedCentralGoogle Scholar
- 61.Ylitalo EB, Thysell E, Jernberg E, Lundholm M, Crnalic S, Egevad L, Stattin P, Widmark A, Bergh A, Wikström P. Subgroups of castration-resistant prostate cancer bone metastases defined through an inverse relationship between androgen receptor activity and immune response. Eur Urol. 2017;71(5):776–87.CrossRefPubMedGoogle Scholar
- 62.Lucia Radici MB, Rita Crinelli, Mauro Magnani. Ubiquitin C gene: structure, function, and transcriptional regulation. Adv Biosci Biotechnol 2013;4:1057–1062.Google Scholar
- 63.Song HM, Lee JE, Kim JH. Ubiquitin C-terminal hydrolase-L3 regulates EMT process and cancer metastasis in prostate cell lines. Biochem Biophys Res Commun. 2014;452(3):722–7.CrossRefPubMedGoogle Scholar
- 64.Jang MJ, Baek SH, Kim JH. UCH-L1 promotes cancer metastasis in prostate cancer cells through EMT induction. Cancer Lett. 2011;302(2):128–35.CrossRefPubMedGoogle Scholar
- 65.Hawkins PG, Santoso S, Adams C, Anest V, Morris KV. Promoter targeted small RNAs induce long-term transcriptional gene silencing in human cells. Nucleic Acids Res. 2009;37(9):2984–95.CrossRefPubMedPubMedCentralGoogle Scholar
- 66.Wahl S, Drong A, Lehne B, et al. Epigenome-wide association study of body mass index, and the adverse outcomes of adiposity. Nature. 2017;541(7635):81–6.CrossRefPubMedGoogle Scholar
- 67.Rahmioglu N, Drong AW, Lockstone H, Tapmeier T, Hellner K, Saare M, Laisk-Podar T, Dew C, Tough E, Nicholson G, Peters M, Morris AP, Lindgren CM, Becker CM, Zondervan KT. Variability of genome-wide DNA methylation and mRNA expression profiles in reproductive and endocrine disease related tissues. Epigenetics. 2017;12(10):897–908.CrossRefPubMedPubMedCentralGoogle Scholar
- 68.Agha G, Houseman EA, Kelsey KT, Eaton CB, Buka SL, Loucks EB. Adiposity is associated with DNA methylation profile in adipose tissue. Int J Epidemiol. 2015;44(4):1277–87.CrossRefPubMedGoogle Scholar
- 69.Turcot V, Tchernof A, Deshaies Y, Pérusse L, Bélisle A, Marceau S, Biron S, Lescelleur O, Biertho L, Vohl MC. LINE-1 methylation in visceral adipose tissue of severely obese individuals is associated with metabolic syndrome status and related phenotypes. Clin Epigenetics. 2012;4(1):10.CrossRefPubMedPubMedCentralGoogle Scholar
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