Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility
The high cost of enzymes is one of the key technical barriers that must be overcome to realize the economical production of biofuels and biomaterials from biomass. Supplementation of enzyme cocktails with lytic polysaccharide monooxygenase (LPMO) can increase the efficiency of these cellulase mixtures for biomass conversion. The previous studies have revealed that LPMOs cleave polysaccharide chains by oxidization of the C1 and/or C4 carbons of the monomeric units. However, how LPMOs enhance enzymatic degradation of lignocellulose is still poorly understood.
In this study, we combined enzymatic assays and real-time imaging using atomic force microscopy (AFM) to study the molecular interactions of an LPMO [TrAA9A, formerly known as TrCel61A) from Trichoderma reesei] and a cellobiohydrolase I (TlCel7A from T. longibrachiatum) with bacterial microcrystalline cellulose (BMCC) as a substrate. Cellulose conversion by TlCel7A alone was enhanced from 46 to 54% by the addition of TrAA9A. Conversion by a mixture of TlCel7A, endoglucanase, and β-glucosidase was increased from 79 to 87% using pretreated BMCC with TrAA9A for 72 h. AFM imaging demonstrated that individual TrAA9A molecules exhibited intermittent random movement along, across, and penetrating into the ribbon-like microfibril structure of BMCC, which was concomitant with the release of a small amount of oxidized sugars and the splitting of large cellulose ribbons into fibrils with smaller diameters. The dividing effect of the cellulose microfibril occurred more rapidly when TrAA9A and TlCel7A were added together compared to TrAA9A alone; TlCel7A alone caused no separation.
TrAA9A increases the accessible surface area of BMCC by separating large cellulose ribbons, and thereby enhances cellulose hydrolysis yield. By providing the first direct observation of LPMO action on a cellulosic substrate, this study sheds new light on the mechanisms by which LPMO enhances biomass conversion.
KeywordsLPMO CBH I AFM Lignocellulose Biomass Cellulose Biorefinery
lytic polysaccharide monooxygenase
a lytic polysaccharide monooxygenase from Trichoderma reesei
a cellobiohydrolase I from Trichoderma longibrachiatum
bacterial microcrystalline cellulose
Non-food plant biomass is a sustainable source of fermentable sugars for the production of biofuels and chemicals [1, 2, 3]. Cellulose, the main component of lignocellulosic biomass, forms rigid microfibrils composed of well-organized linear β-1,4-glucan chains. Due to its homogeneity and abundance, cellulose is of major interest in producing monomeric sugars for biorefineries [2, 4, 5]. However, the cellulose chains in a microfibril are strongly held together by hydrogen bond networks and van der Waals forces, so that the majority of these chains are not readily accessible to cellulases. In addition, in plant biomass, microfibrils are organized into closely associated bundles and are embedded in a non-cellulosic matrix of lignin and hemicelluloses, which further reduce the efficiency of biomass conversion [2, 6].
The most widespread method of cellulose conversion requires three types of cellulases to act synergistically to convert cellulose to glucose: endo-β-1,4-glucanases (EGs) cleave the internal bonds in the cellulose chain, cellobiohydrolases (CBHs) processively hydrolyze cellulose from the chain ends to produce cellobiose, and β-glucosidases (BGs) hydrolyze cellobiose to glucose . Lytic polysaccharide monooxygenases (LPMOs) are a recently discovered class of enzymes that stimulate biomass hydrolysis and hence improve the efficiency of biomass conversion [8, 9, 10, 11]. Unlike cellulases that cleave glycosidic bonds by hydrolysis , LPMOs are copper-dependent enzymes that lyse polysaccharide chains by oxidation at either the C1 or the C4 carbon of the glucose unit in the presence of an external electron donor [13, 14, 15, 16, 17]. However, the mechanism by which the addition of LPMOs to cellulase mixtures enhances the overall yield of glucose has not been clearly elucidated.
Atomic force microscopy (AFM), especially high-speed AFM, can image with sub-nanometer resolution under aqueous conditions at video rate. AFM has been used to visualize the interactions between enzymes and substrates in real time . AFM has also been used to image the structural changes of cellulose after treatment by LPMO showing disintegrating and fibrillation of cellulose microfibrils . However, how the interaction of LPMO with the cellulose surface causes these changes is still not well understood. In this study, we use high-speed AFM to monitor in situ interactions of an LPMO (TrAA9A, formerly known as TrCel61A) and a CBH I (TlCel7A), alone and together, with bacterial microcrystalline cellulose (BMCC). Our goal was to visualize in real time the molecular motion of LPMO and its effects on the structure of the cellulose surface, to deepen our understanding of LPMO's role in cellulose hydrolysis.
Results and discussion
LPMO has been shown to break cellulose chains by oxidation at the C1 and/or C4 position(s) [4, 21, 22]. To further confirm the specific activity of TrAA9A, we used mass spectrometry (MS) to analyze the soluble products after 72 h incubation of TrAA9A with BMCC, and detected small amounts of oxidized forms of cellobiose and cellotriose (Additional file 1: Figure S1), consistent with the previous reports .
Continuous imaging revealed that TrAA9A molecules initially bound randomly to the BMCC surface, and individual TrAA9A molecules exhibited a “stop-and-go” behavior. Interestingly, the enzyme molecules stayed in approximately the same position for a much longer time than they spent moving on the cellulose. TrAA9A moved at 0.25 ± 0.13 nm/s (n = 40) in three patterns: in both directions along one cellulose ribbon, across one ribbon, or moving from one ribbon to another (Fig. 2a–c, respectively; details in Additional file 2: Video S1, Additional file 3: Video S2, Additional file 4: Video S3). The observed speed of TrAA9A was one order of magnitude slower than previously observed for CBHI moving on Valonia microcrystalline cellulose (TrCel7A) . This might be related to the slow rate of oxidization catalyzed by TrAA9A . Interestingly, when the imaging period was extended to ~7 h, some TrAA9A molecules seemed to form into groups that moved together towards a particular cellulose ribbon where eventually many molecules accumulated (Fig. 2c; Additional file 4: Video S3).
Previously, AFM imaging revealed that TrCel7A binds and reacts on the hydrophobic face of crystalline cellulose  and moves processively from the reducing end of the glucan chain to the non-reducing end . In contrast, TrAA9A moves in both directions along a ribbon and also across ribbons. The different binding and movement patterns of TrAA9 and TlCel7A observed in this study can be explained by the differences in their molecular structures. The binding face of LPMO is flat [14, 24], whereas CBHs like TlCel7A adopt a tunnel-shape active site that can hold several glucose residues of the cellulose chain and thereby constrain movement in a linear fashion [21, 22, 27, 28].
After 4 h of incubation, the resulting cellulose microfibrils were significantly thinner when treated by TrAA9A together with TlCel7A (Fig. 5a) than when treated by TrAA9A alone (Fig. 4). Interestingly, cellulose hydrolysis by the cellulase mixture composed of TlCel7A, EG, and BG increased from 79 to 87% when using TrAA9A-pretreated BMCC, compared with untreated BMCC (Additional file 11: Figure S5). This enhancement is similar to the results for TrAA9A and TlCel7A added together to untreated BMCC, suggesting that the synergy of TrAA9A and cellulases during cellulose hydrolysis may not be attributed to the direct molecular interaction between these enzymes.
We used high-speed AFM imaging to visualize in real-time the enzymatic hydrolysis of cellulose by cellulases and an LPMO, TrAA9A, in this study. Our results are consistent with the previous studies, but further show that cellulases, i.e., CBHs and EGs, are responsible for the majority of cellulose hydrolysis, and that LPMOs promote their efficiency. We observe that LPMO reacts specifically to disintegrate large cellulose ribbons into small microfibrils with little oxidized sugar products, whereas CBHI has been considered to be a “peel-off” process [26, 31]; we hypothesize that LPMO and CBHI may attack different structures of cellulose. BMCC ribbons are considered to contain amorphous cellulose and CEFs that are composed of well-organized cellulose glucan chains [29, 30]. It is likely that LPMO attacks only the amorphous proportion of cellulose and increases the surface accessibility of CEFs for cellulase enzymes. If this is true, LPMO would not enhance cellulase activity when cellulose accessibility is already at a maximum. Indeed, it has been previously demonstrated that the degree of LPMO enhancement is negatively correlated with cellulose accessibility . Similarly, LPMO has been found to increase the degradation of insoluble xylan , one of the major matrix polysaccharides in the plant cell wall, suggesting that LMPO can oxidize sugars in different amorphous polysaccharides, thus exposing the well-organized cellulose microfibrils to be hydrolyzed by cellulases.
All chemicals and reagents, unless specifically noted, were purchased from Sigma-Aldrich (St. Louis, MO).
Bacterial strain and medium
Gluconacetobacter xylinus (also known as Komagataeibacter xylinus) strain ATCC 53524 from the American-Type Culture Collection (Manassas, VA) was maintained on Hestrin and Schramm (HS) medium containing 1.5% agar. HS medium contains 20-g/L glucose, 5-g/L peptone, 5-g/L yeast extract, 2.7-g/L Na2HPO4, and 1.15-g/L citric acid monohydrate. The pH was adjusted to 6.0 with NaOH or HCl.
TlCel7A from Trichoderma longibrachiatum (Catalog No. E-CBHI) was purchased from Megazyme, Ltd., Bray, Ireland. TrAA9A (GenBank CAA71999), endo-β-1,4-glucanase (EG; AAA34212) and β-glucosidase (BG; AAA18473) from T. reesei were expressed in Pichia pastoris as previously described . Pronase E (Catalog No. P2714) was purchased from Sigma-Aldrich (St. Louis, MO).
Production of BMCC
The bacterial microcrystalline cellulose (BMCC) was produced based on the previous study with modifications [34, 35]. HS medium (50 mL) in a 250-mL flask was inoculated with G. xylinus colonies and incubated at 30°C for 1–3 days in static until cellulose pellicle was visible. The culture was vigorously shaken for 30 min to release active cells embedded in the pellicle. Then, 450-mL fresh HS medium in a plastic tray (18 × 6 × 2.5 in.) was inoculated with the culture broth and incubated in stationary for another 3–5 days until the cellulose pellicle was grown on the entire medium surface.
The cellulose pellicle was collected by filtering through 2-layer Miracloth. Then, the pellicle was washed repeatedly with distilled deionized water (ddH2O) to rinse off the medium as much as possible. Washed cellulose was boiled in 1% NaOH with stirring for 30 min to remove remaining bacteria. Finally, alkali-treated cellulose was washed several times with ddH2O until the water pH reached 7.0, freeze-dried, and cut to 1-cm strip for enzymatic degradation. Native BMCC without alkali treatment was stored in 0.02% sodium azide at 4 °C for AFM imaging.
Preparation of BMCC samples
The BMCC sample was prepared by a hand-cutting of fresh and never dried BMCC film. Only thin layers with approximated 10–50-μm thickness were used in the experiment. A bright field microscopy was used to select a thinner sample with relative uniform surface. After washing by deionized water several times and placed on a poly-lysine-coated glass slide (Thermo Fisher Scientific Inc, Waltham, MA, USA) with deionized water. The samples were kept in the water for all of the imaging and measurement process.
All the AFM experiments were conducted at room temperature on a Dimension AFM with Nanoscope controller V (Fastscan, Bruker Nano, Santa Barbara, CA. USA) with an acoustic and vibration isolation system. Probes we used were SCANASYST-FLUID+ (Bruker, Camarillo, CA USA) for imaging under fluid. The AFM operation software (Nanoscope V9.1) was used to control the scan size, setpoint, and gain. Before AFM imaging, the scanner has been carefully calibrated using calibration kit (Bruker, Camarillo, CA, USA) to make sure that all the measurements are very close to their actual value. To dynamically capture the movement of enzyme molecules, the scan rate was normally set as 10–20 Hz for the continuous observation with low resolution (256 × 128 pixels or 128 × 64 pixels). However, when we conducted the imaging on single TrAA9A penetration experiment, we lowered the scan rate to 2 Hz to obtain images with better resolution. For static observations, the scan rate was 1 Hz with the resolution of 1024 × 1024 pixels.
AFM measurement and image processing
All off-line data analysis was based on the Nanoscope Analysis v1.8 software (Bruker Nano, Santa Barbara, CA. USA). The height and peak force error images were analyzed using plane fit filter at one order for images presented in all figures. The color bar was manually modified according to the best presence of each image. The images used for height and width measurement were raw data without any processing and all width and height measurements were conducted with the “section” function in the Nanoscope Analysis software.
Preparation of TrAA9A-treated BMCC
2-mg/mL BMCC was incubated with 100-μg/mL TrAA9A, 1-mM L-ascorbic acid, and 0.02% sodium azide for 72 h in 50-mM pH 4.8 sodium acetate buffer. All reactions in triplicate were conducted at 150 rpm, 50 °C in shaking incubator. After 72-h incubation, BMCC residue was recovered by filtering through two layers of Miracloth (Calbiochem, San Diego, CA). Filtrates from all reactions were saved for TrAA9A product analysis. Then, TrAA9A remained on cellulose residue was removed according to the previous study with modifications . The residue was first washed several times with ddH2O. Then, it was incubated with 10-mg/mL Pronase E in 50-mM pH 7.5 Tris buffer overnight at 37 °C, 100 rpm for complete proteolysis of remaining TrAA9A. Next, BMCC residue was collected by filtering through two layers of Miracloth, washed with ddH2O, 1 M NaCl, and ddH2O again to remove Pronase E. Finally, the BMCC residue was freeze-dried and stored at 4 °C.
TrAA9A product analysis by LC–MS
Filtrate obtained after 72-h reaction with TrAA9A was centrifuged in 70% ethanol for 20 min at 4 °C to precipitate TrAA9A and supernatant was collected. Filtrate of BMCC incubated under the same condition without TrAA9A was also prepared in the same way for mass spectrometry analysis. Samples were analyzed on a Waters Xevo G2-XS Q-TOF system coupled to a Waters I-Class UPLC system. Carbohydrates were separated by an ACQUITY UPLC BEH Amide column (2.1 × 100 mm, 1.7 μm) maintained at 40 °C, with the injection volume at 10 μL. Solvent A was 10-mM ammonium formate, and solvent B was 100% acetonitrile. The solutes were eluted at 0.2 mL/min starting at 95% B, followed by a linear gradient to 35% B over 14 min. The proportion of solvent B was maintained at 35% for 2 min and then increased back to 95% and kept for 4 min for re-equilibration.
The operation condition for mass spectrometer was capillary voltage of 3.0 kV, sample cone voltage of 80 V, source temperature of 100 °C, desolvation temperature of 350 °C, and desolvation gas flow of 600 L/h. Mass spectra were acquired in positive ion mode across the 50–2000-m/z range. Data processing was performed using the MassLynx software (version 4.1, Waters).
Enzymatic degradation of BMCC
To test the synergism between TrAA9A and TlCel7A on cellulose degradation, 2-mg/mL BMCC was incubated with 34-μg/mL TlCel7A and 6-μg/mL TrAA9A, alone or together, in 10-mL reactions containing 1-mM L-ascorbic acid and 0.02% sodium azide. Reactions were conducted in triplicate in 50-mM sodium acetate, pH 4.8, for 72 h at 150 rpm, 50 °C. To detect cellulose conversion rate, 100-μL supernatant was incubated with 40-μg/mL β-glucosidase in 50-mM pH 4.8 sodium acetate buffer for 30 min at 50 °C. Free glucose was measured by enzyme-linked colorimetry as described .
To test the digestibility of TrAA9A-treated cellulose, TrAA9A-treated BMCC was compared against untreated BMCC for enzymatic hydrolysis at pH 4.8. For all reactions, cellulose loading was 2 mg/mL and cellulase mixture loading 100 μg/mL, which contained TlCel7A, EG, and BG with 6:3:1 ratio. Each reaction in triplicate was carried at 150 rpm, 50 °C in 0.02% sodium azide. Glucose yield was measured during 72-h incubation.
SYD designed the study and guided the experiment; BS and BL performed the experiments, analyzed data, and drafted the manuscript equally. XW, WS, SP, AF, and CC processed, analyzed the data, and modify the manuscript. JDW and SJS reviewed the data and helped write the manuscript. All authors read and approved the final manuscript.
We would like to thank Dr. Daniel Jones and Dr. Tony Schilmiller, MSU Mass Spectrometry Facility, for their help with the MS experimental design.
The authors declare that they have no competing interests.
Availability of data and materials
The data sets generated and/or analyzed during the present study are included in this published article and are available from BS and SD on reasonable request.
Ethics approval and consent to participate
This work was funded by the US National Science Foundation (DMR-BMART-1206908), the U.S. Department of Energy Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494) and Office of Biological and Environmental Research (DE-SC0012411). Additional funding was provided by grant DE-FG02-91ER200021 to the MSU-DOE Plant Research Laboratory from the U.S. Department of Energy, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences and Biosciences.
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