Skip to main content
Springer Nature Link
Account
Fig. 3 | Malaria Journal

Fig. 3

From: Unravelling the mode of action of the Tres Cantos Antimalarial Set (TCAMS): investigating the mechanism of potent antimalarial compounds potentially targeting the human serotonin receptor

Fig. 3

Survival curves for TCAMS compounds hypothetically targeting serotonin receptors. Asynchronous parasites were incubated with the compounds TCMDC-139482, TCMDC-139634, TCMDC-139786, TCMDC-139803, TCMDC-139813, TCMDC-140136, TCMDC-140219, TCMDC-142310, TCMDC-142311, TCMDC-139860, TCMDC-135267, TCMDC-135301, TCMDC-135385, TCMDC-135435 and TCMDC-135459 (0. 0019–2 µM), with an initial parasitaemia of 0.3% at a haematocrit of 2%. Parasitaemia at the end of 72 h of incubation was quantified via flow cytometry, and the data generated by the cytometer were analysed via FlowJo V10.9.0 software. Parasitaemia values were tabulated in Prism GraphPad software to construct survival curves. IC50 values were first calculated in µM and then converted to nM and are presented above the survival curves for each compound. The experiments were performed in three independent biological replicates. The error bars represent the SEM

Back to article page