Potent antiplasmodial extracts and fractions from Terminalia mantaly and Terminalia superba
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Abstract
Background
The emergence and spread of malaria parasites resistant to artemisinin-based combination therapy stresses the need for novel drugs against malaria. Investigating plants used in traditional medicine to treat malaria remains a credible option for new anti-malarial drug development. This study was aimed at investigating the antiplasmodial activity and selectivity of extracts and fractions from Terminalia mantaly and Terminalia superba (Combretaceae) that are used in Cameroon to treat malaria.
Methods
Twelve methanolic (m) and water (w) extracts obtained by maceration of powdered dried leaves (l), stem bark (sb) and root (r) of Terminalia mantaly (Tm) and Terminalia superba (Ts) and 12 derived fractions of hexane, chloroform, ethyl acetate and 4 final residues of selected extracts were assessed for antiplasmodial potential in vitro against the chloroquine-resistant PfINDO and the chloroquine-sensitive Pf3D7 strains of Plasmodium falciparum using the SYBR green I-based fluorescence assay. The cytotoxicity of potent extracts and fractions was evaluated in vitro using the MTT assay on HEK239T cell line.
Results
The antiplasmodial IC50 of extracts from both plants ranged from 0.26 to > 25 µg/mL. Apart from the extracts Tmrm and Tsrw that exerted moderate antiplasmodial activities (IC50: 5–20 µg/mL) and Tmrw that was found to be non-active at the tested concentrations (IC50 > 25 µg/mL), all other tested crude extracts exhibited potent activities with IC50 < 5 µg/mL. The aqueous extracts from the stem bark of Terminalia mantaly (Tmsbw) and the leaf of Terminalia superba (Tslw) displayed the highest antiplasmodial activities (IC50: 0.26–1.26 µg/mL) and selectivity (SI > 158) on both resistant PfINDO and sensitive Pf3D7 strains. Four fractions upon further extraction with chloroform and ethyl acetate (TmlwChl, TmsbwChl, TmsbwEA, TsrmEA) afforded from three selected crude extracts (Tmlw, Tmsbw, Tsrm) exhibited highly potent activities against both P. falciparum strains (IC50 < 2 µg/mL) and high selectivity (SI > 109).
Conclusions
The results achieved in this work validate the reported traditional use of Terminalia mantaly and Terminalia superba to treat malaria. Moreover, the highly potent and selective fractions warrant further investigation to characterize the active antiplasmodial principles and progress them to rodent malaria models studies if activity and selectivity are evidenced.
Keywords
Plasmodium falciparum Antiplasmodial Selectivity Terminalia mantaly Terminalia superbaAbbreviations
- CC50
50% cell cytotoxic concentration
- Chl
chloroform
- DMEM
Dulbecco’s Modified Eagle’s Medium
- DMSO
dimethyl sulfoxide
- EA
ethyl acetate
- EDTA
ethylene diamine tetra acetate
- H
hexane
- HEK239T
human embryonic kidney cells 239T
- HEPES
2-[4-(2 hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
- HNC
Herbier National du Cameroun
- IC50
50% inhibitory concentration
- IC50Pf3D7
50% inhibitory concentration on Plasmodium falciparum 3D7 strain
- IC50PfINDO
50% inhibitory concentration on Plasmodium falciparum INDO strain
- IMPM
Institute for Medical Research and Medicinal Plants Studies
- l
leaf
- m
methanol
- mL
milliliter
- mM
millimolar
- MTT
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
- Pf3D7
Plasmodium falciparum 3D7 strain
- PfINDO
Plasmodium falciparum INDO strain
- r
root
- R3
final residue
- RI
resistance index
- RPMI
Roswell Park Memorial Institute Medium
- sb
stem bark
- SD
standard deviation
- SI
selectivity index
- SIPf3D7
selectivity index on Plasmodium falciparum 3D7 strain
- SIPfINDO
selectivity index on Plasmodium falciparum INDO strain
- Tmlm
Terminalia mantaly leaf methanol extract
- Tmlw
Terminalia mantaly leaf water extract
- TmlwChl
Terminalia mantaly leaf water extract chloroform fraction
- TmlwEA
Terminalia mantaly leaf water extract ethyl acetate fraction
- TmlwH
Terminalia mantaly leaf water extract hexane fraction
- TmlwR3
Terminalia mantaly leaf water extract final residue
- Tmrm
Terminalia mantaly root methanol extract
- Tmrw
Terminalia mantaly root water extract
- Tmsbm
Terminalia mantaly stem bark methanol extract
- Tmsbw
Terminalia mantaly stem bark water extract
- TmsbwChl
Terminalia mantaly stem bark water extract chloroform fraction
- TmsbwEA
Terminalia mantaly stem bark water extract ethyl acetate fraction
- TmsbwH
Terminalia mantaly stem bark water extract hexane fraction
- TmsbwR3
Terminalia mantaly stem bark water extract final residue
- Tslm
Terminalia superba leaf methanol extract
- Tslw
Terminalia superba leaf water extract
- TslwChl
Terminalia superba leaf water extract chloroform fraction
- TslwEA
Terminalia superba leaf water extract ethyl acetate fraction
- TslwH
Terminalia superba leaf water extract hexane fraction
- TslwR3
Terminalia superba leaf water extract final residue
- Tsrm
Terminalia superba root methanol extract
- TsrmChl
Terminalia superba root methanol extract chloroform fraction
- TsrmEA
Terminalia superba root methanol extract ethyl acetate fraction
- TsrmH
Terminalia superba root methanol extract hexane fraction
- TsrmR3
Terminalia superba root methanol extract final residue
- Tsrw
Terminalia superba root water extract
- Tssbm
Terminalia superba stem bark methanol extract
- Tssbw
Terminalia superba stem bark water extract
- w
water
Background
Malaria is the world’s most important protozoan disease. In 2015, an estimated 214 million malaria cases occurred worldwide with an approximated 438,000 deaths. Sub-Saharan Africa is the most affected region based on statistics, with 88% of the overall malaria cases and 90% of all deaths [1]. In Africa, malaria constitutes over 10% of overall disease burden, accounting for 40% of public health expenditure, 30–50% of in-patient hospital admissions and up to 50% of out-patient visits in endemic areas, and thus represents a major hindrance to the socio-economic development [2].
Cameroon is amongst the most affected countries where malaria is the first major cause of morbidity and mortality amongst the most vulnerable patient groups such as children under 5 years of age, pregnant women, people living with HIV/AIDS and the poor [3]. In 2014, the whole Cameroonian population was at risk of malaria with 71% living in high transmission areas. Plasmodium falciparum is the causative agent of all reported cases of malaria in Cameroon [1]. Overall, thousands of people continue to die from malaria each year and despite extensive efforts to control the disease, it remains a major public health threat [4, 5]. With persistent severe malarial morbidity and increasing resistance to malaria drugs, including the recently introduced, first-line, artemisinin-based combination therapy (ACT) [6, 7], there is a compelling need for new and improved treatments for malaria. History reveals that medicinal plants have always been an important source of chemotherapeutic agents, and indigenous healthcare systems have always played a vital role in the management of community health and discovery of novel chemotherapeutic agents against malaria. For example, two of the most important anti-malarial drugs, namely quinine and artemisinin, have their respective origins in the medicinal plants Cinchona officinalis and Artemisia annua [8]. In Cameroon, pharmacopeia plants are widely used to treat malaria and several other diseases, particularly in remote areas where access to standard treatments is limited. Terminalia mantaly and Terminalia superba have been reported in such areas in Cameroon as sources of treatment for various diseases, including malaria and/or related symptoms [9]. However, little is known about the antiplasmodial activity of extracts from these plants. This study was therefore designed to investigate the antiplasmodial potential and cytotoxicity of extracts and fractions from leaves, stem back and roots of Terminalia mantaly and Terminalia superba.
Methods
Plants collection, extraction and fractionation
Leaf, stem bark and root samples were collected from Terminalia mantaly and Terminalia superba during August and September 2014 in Nkolbisson and Ngoa-Ekelle, Yaoundé, Cameroon. Plants were identified at the National Herbarium of Cameroon, Yaoundé where voucher specimens were deposited under the reference numbers 64212/HNC [Terminalia mantaly (Tm)] and 55030/HNC [Terminalia superba (Ts)], respectively.
The plant samples were air-dried (2 kg dry weight) and ground into fine powder using an electric mill (Hammer Mill, Leabon 9FQ, Zhengzhou, PRC). One kg of each plant part powder was separately macerated in 10 L of distilled water and methanol, respectively, for 72 h at room temperature (27–29 °C). The methanol macerates were filtered and filtrates evaporated using a rotary evaporator (Rotavapor, BUCHI 071, Switzerland) at 65 °C. The aqueous extracts were lyophilized at the Laboratory of Phytochemistry, Institute for Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, Cameroon using a Virtis Wizard 2.0 Freeze Dryer Lyophilizer: Model: XLS-70. The dried crude extracts were subsequently subjected to antiplasmodial screening in vitro against the resistant PfINDO and sensitive Pf3D7 strains. Crude extracts with potent antiplasmodial activity and high selectivity index were selected and subjected to successive solid–liquid solvent extractions. Cytotoxicity of extracts and fractions was assessed on HEK239T cells using MTT assay [10].
Scheme showing the successive solid–liquid extractions of the crude extracts using organic solvents
In vitro cultivation of Plasmodium falciparum
Chloroquine-sensitive 3D7 (Pf3D7) and resistant INDO (PfINDO) strains of P. falciparum were maintained at the Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, New Delhi, India, and used for in vitro blood stage testing of antiplasmodial activity of the plant extracts and fractions. Plasmodium falciparum culture was maintained according to the method described by Trager and Jensen [11] with minor modifications [12], in fresh O positive human erythrocytes suspended at 4% haematocrit in RPMI 1640 16.2 g/L (Sigma-Aldrich, New Delhi, India) containing 25 mM HEPES, 11.11 mM glucose, 0.2% sodium bicarbonate (Sigma-Aldrich, New Delhi, India), 0.5% albumax I (Gibco, Waltham, MA USA), 45 mg/L hypoxanthine (Sigma-Aldrich, New Delhi, India), and 50 mg/L gentamicin (Gibco, Waltham, MA USA) and incubated at 37 °C under a gas mixture of 5% O2, 5% CO2 and 90% N2. Every day infected erythrocytes were transferred into fresh complete medium to propagate the culture. At parasitaemia > 10%, the cultures were diluted into healthy red blood cells to reduce % parasitaemia to 1–2% so as to maintain the cultures in stress free conditions.
Dilution of crude extracts, fractions and positive control
Stock solutions of plant extracts and fractions were prepared at 1 mg/mL in dimethyl sulfoxide (DMSO) while chloroquine (Sigma-Aldrich, New Delhi, India) stock solution used as standard drug was prepared in water (Milli-Q grade, The Netherlands) at 1 mM. All stocks solutions were then diluted in 96-well, round-bottom, tissue culture-grade plates (Corning, USA) with fresh RPMI 1640 culture medium to achieve the required concentrations for testing. In all cases, except for chloroquine (positive control), the final solution contained 0.4% DMSO, which was found to be non-toxic to the parasite. Extracts and fractions were tested at concentrations ranging from 0.10 to 25 μg/mL, and chloroquine at 1 µM highest concentration.
In vitro antiplasmodial assays
Crude extracts and fractions were evaluated for antiplasmodial activity against P. falciparum INDO and 3D7 strains. For drug screening, the SYBR green I-based fluorescence assay was set up as described by Smilkstein et al. [13]. Precisely, 100 μL of Sorbitol-synchronized parasites [14] were incubated under normal culture conditions (37 °C, 5% CO2, 5% O2, 90% N2) at 1% parasitaemia and 2% haematocrit in flat-bottomed, 96-well plates (Corning, USA) in the absence or presence of increasing concentrations of crude extracts or fractions for 48 h. Chloroquine (Sigma-Aldrich, New Delhi, India) was used as positive control, while 0.4% (v/v) DMSO was used as the negative control. Upon incubation, 100 μL of SYBR green I lysis buffer (Tris (20 mM; pH 7.5), EDTA (5 mM), saponin (0.008%, w/v), and Triton X-100 (0.08%, v/v))was added to each well and mixed gently twice, then incubated in dark at 37 °C for 1 h. Fluorescence was then measured with a Victor fluorescence multiwell plate reader (Perkin Elmer, Waltham, MA, USA) with excitation and emission wavelength bands centred at 485 and 530 nm, respectively. The fluorescence counts were plotted against drug concentration and the 50% inhibitory concentration (IC50) was determined by analysis of dose–response curves using non-linear regression. Resistance indexes were calculated as RI = IC50 PfINDO/IC50Pf3D7. Results were validated microscopically by examination of Giemsa-stained smears of extract-treated/untreated parasite cultures.
Assessment of the cytotoxicity of active extracts and fractions on HEK239T cells
The cytotoxic effect of antiplasmodial extracts and fractions was assessed using the MTT assay [10], targeting HEK239T cells cultured in complete medium containing 13.5 g/L DMEM (Gibco, Waltham, MA USA), 10% fetal bovine serum (Gibco, Waltham, MA USA), 0.21% sodium bicarbonate (Sigma-Aldrich, New Delhi, India) and 50 μg/mL gentamicin (Gibco, Waltham, MA USA). Essentially, HEK239T cells at 5 × 103 cells/200 μL/well were seeded into 96-well, flat-bottomed, tissue culture plates (Corning, USA) in complete medium. Fifty µL of serially diluted extracts and fractions solutions (≤ 200 µg/mL) were added after 24 h of seeding and then incubated for 48 h in a humidified atmosphere at 37 °C and 5% CO2. DMSO was added as positive inhibitor at 10% v/v. Twenty µL of a stock solution of MTT (5 mg/mL in 1× phosphate-buffered saline) were added to each well, gently mixed and incubated for an additional 4 h. After spinning the plate at 1500 rpm for 5 min, the supernatant was carefully removed and 100 μL of DMSO (stop agent) was added. Formazan formation was read on a microtiter plate reader (Versa Max Microplate Reader, Molecular Devices, USA) at 570 nm. The 50% cytotoxic concentrations (CC50) of extracts and fractions were determined by analysis of dose–response curves. Selectivity indices (CC50/IC50) were calculated for each extract and fraction.
Results
The present study was designed to assess the biological properties of Terminalia mantaly and Terminalia superba as sources of antiplasmodial agents with potential to be further investigated for anti-malarial drug development. The results achieved are summarized and discussed below.
Antiplasmodial activity and cytotoxicity of crude extracts from Terminalia mantaly and Terminalia superba
Crude extracts preparation yields and activity parameters
| Plant | Part | Solvent | Extract code | Extraction yield (%) | IC50 ± SD (µg/mL) | RI | CC50 (µg/mL) | Selectivity Index | ||
|---|---|---|---|---|---|---|---|---|---|---|
| PfINDO | Pf3D7 | PfINDO | Pf3D7 | |||||||
| T. mantaly | Leaf | Water | Tmlw | 35.50 | 2.09 ± 0.06 | 2.66 ± 0.31 | 0.79 | > 200 | > 95.69 | > 75.19 |
| Methanol | Tmlm | 19.54 | 2.69 ± 0.04 | 2.61 ± 0.43 | 1.03 | > 200 | > 74.35 | > 76.63 | ||
| Stem bark | Water | Tmsbw | 19.93 | 0.26 ± 0.02 | 1.03 ± 0.04 | 0.25 | > 200 | > 769.23 | > 194.17 | |
| Methanol | Tmsbm | 19.32 | 3.63 ± 0.50 | 2.91 ± 0.12 | 1.25 | > 200 | > 55.10 | > 68.73 | ||
| Root | Water | Tmrw | 07.45 | > 25 | > 25 | – | – | – | – | |
| Methanol | Tmrm | 09.01 | 7.01 ± 0.82 | 5.04 ± 0.68 | 1.39 | > 200 | > 28.53 | > 39.68 | ||
| T. superba | Leaf | Water | Tslw | 17.18 | 0.57 ± 0.06 | 1.26 ± 0.12 | 0.45 | > 200 | > 350.88 | > 158.73 |
| Methanol | Tslm | 12.07 | 3.38 ± 0.54 | 2.13 ± 0.13 | 1.59 | > 200 | > 59.17 | > 93.90 | ||
| Stem bark | Water | Tssbw | 14.15 | 3.70 ± 0.03 | 1.42 ± 0.09 | 2.61 | > 200 | > 54.05 | > 140.85 | |
| Methanol | Tssbm | 10.35 | 2.68 ± 0.20 | 1.85 ± 0.13 | 1.44 | > 200 | > 74.63 | > 108.11 | ||
| Root | Water | Tsrw | 04.54 | 18.88 ± 0.14 | 16.43 ± 0.14 | 1.15 | – | – | – | |
| Methanol | Tsrm | 02.27 | 2.38 ± 0.08 | 2.28 ± 0.20 | 1.04 | > 200 | > 84.03 | > 87.72 | ||
| Reference drug | Chloroquine (µM) | – | 0.40 ± 0.00 | 0.04 ± 0.01 | 10.00 | – | – | – | ||
Dose–response curves of Terminalia mantaly and Terminalia superba crude extracts on Plasmodium falciparum INDO and 3D7 strains (Tm: Terminalia mantaly; Ts: Terminalia superba; l: leaf; sb: stem bark; r: roots; w: water; m: methanol)
Results from Table 1 show that plant extraction yields varied from 7.45 to 35.50% for Terminalia mantaly parts and from 2.27 to 17.18% for Terminalia superba parts. Yields were dependent upon plant species, parts and solvent of extraction. In general, leaves as source and water as extractant gave higher yields than was the case with methanol. Water is environment-friendly, making it an ideal and cheap solvent for the extraction of bioactive and safe principles from any source.
The antiplasmodial activity (expressed as IC50) of crude extracts ranged from 0.26 to > 25 µg/mL with nine extracts showing very promising potency (IC50 < 5 µg/mL). The aqueous extract from Terminalia mantaly stem bark (Tmsbw) showed the best antiplasmodial activity and selectivity, respectively, on PfINDO and Pf3D7 strains with IC50 values of 0.26 µg/mL (SIPfINDO > 769.23) and 1.03 µg/mL (SIPf3D7 > 194.17). For Terminalia superba, the aqueous extract from leaves (Tslw) also showed high antiplasmodial activity and selectivity with IC50 values of 0.57 µg/mL (SIPfINDO > 350.88) and 1.26 µg/mL (SIPf3D7 > 158.73) on PfINDO and Pf3D7 strains, respectively.
With most values hovering around one, resistance index data on crude samples (Table 1) suggests that the antiplasmodial metabolites present in the plant extracts may be equipotent against both chloroquine-sensitive and -resistant strains of P. falciparum. However, Tmsbw (RI: 0.25), and Tslw (RI: 0.45) were clearly three to fourfold more active against PfINDO than Pf3D7, respectively, suggesting interesting possibilities of unique and novel drug targets in the resistant strain. The selectivity indices of crude extracts ranging from > 28 to > 769 µg/mL suggest that the crude extracts not only exhibit potent antiplasmodial potency but also possess great selectivity in their action.
Antiplasmodial activity and cytotoxicity of fractions from the most promising extracts
Four crude extracts showing promising antiplasmodial activity profile were selected and fractionated via successive solid–liquid extractions using three different organic solvents (n-hexane, chloroform, ethyl acetate). These were the aqueous extracts from leaf and stem bark of Terminalia mantaly (Tmlw: IC50PfINDO: 2.09 µg/mL, SIPfINDO > 95; IC50Pf3D7: 2.66 µg/mL, SIPf3D7 > 75; and Tmsbw: IC50PfINDO: 0.26 µg/mL, SIPfINDO > 769; IC50Pf3D7: 1.03 µg/mL, SIPf3D7 > 194), and the aqueous and methanolic extracts from leaf and root of Terminalia superba (Tslw: IC50PfINDO: 0.57 µg/mL, SIPfINDO > 350; IC50Pf3D7: 1.26 µg/mL, SIPf3D7 > 158; and Tsrm: IC50PfINDO: 2.38 µg/mL, SIPfINDO > 84; IC50Pf3D7: 2.28 µg/mL, SIPf3D7 > 87).
Yields of crude extracts fractionation and activity parameters
| Plant | Crude extract | Solvent | Fraction code | Fraction yield (%) | IC50 ± SD (µg/mL) | RI | CC50 (µg/mL) | Selectivity index | ||
|---|---|---|---|---|---|---|---|---|---|---|
| PfINDO | Pf3D7 | PfINDO | Pf3D7 | |||||||
| T. mantaly | Tmlw | Hexane | TmlwH | 0 | – | – | – | – | – | – |
| Chl | TmlwChl | 20.78 | 0.36 ± 0.01 | 0.96 ± 0.01 | 0.37 | > 200 | > 555.55 | > 208.33 | ||
| EA | TmlwEA | 2.54 | 6.74 ± 0.49 | 6.88 ± 0.58 | 0.98 | > 200 | > 29.67 | > 29.07 | ||
| – | TmlwR3 | 43.79 | > 25 | > 25 | – | – | – | – | ||
| Tmsbw | Hexane | TmsbwH | 0.16 | 4.50 ± 0.24 | 4.40 ± 0.27 | 1.02 | > 200 | > 44.44 | > 45.45 | |
| Chl | TmsbwChl | 0.67 | 0.56 ± 0.05 | 1.12 ± 0.07 | 0.5 | > 200 | > 357.14 | > 178.57 | ||
| EA | TmsbwEA | 1.26 | 0.68 ± 0.14 | 1.35 ± 0.42 | 0.5 | > 200 | > 294.11 | > 148.15 | ||
| – | TmsbwR3 | 31.35 | 2.80 ± 0.21 | 2.44 ± 0.16 | 1.15 | > 200 | > 71.43 | > 8.97 | ||
| T. superba | Tslw | Hexane | TslwH | 0.7 | 14.09 ± 1.88 | 6.99 ± 0.68 | 2.02 | 126.03 | > 14.19 | > 28.61 |
| Chl | TslwChl | 4.74 | > 25 | > 25 | – | – | – | – | ||
| EA | TslwEA | 4.47 | 6.89 ± 0.94 | 7.06 ± 0.76 | 0.97 | > 200 | > 29.03 | > 28.33 | ||
| – | TslwR3 | 61.9 | > 25 | > 25 | – | – | – | – | ||
| Tsrm | Hexane | TsrmH | 19.36 | > 25 | > 25 | – | – | – | – | |
| Chl | TsrmChl | 5.54 | 2.26 ± 0.09 | 4.93 ± 0.73 | 0.46 | > 200 | > 88.49 | > 40.57 | ||
| EA | TsrmEA | 6.18 | 1.82 ± 0.04 | 1.65 ± 0.24 | 1.1 | > 200 | > 109.89 | > 121.21 | ||
| – | TsrmR3 | 63.82 | 5.22 ± 0.26 | 4.70 ± 1.47 | 1.11 | > 200 | > 38.31 | > 42.55 | ||
| Reference drug | Chloroquine (µM) | – | 0.40 ± 0.00 | 0.04 ± 0.00 | 10 | – | – | – | ||
a, b Dose-response curves of fractions from Terminalia mantaly and Terminalia superba on Plasmodium falciparum INDO and 3D7 strains (Tm: Terminalia mantaly; Ts: Terminalia superba; Tmlw: Terminalia mantaly leaf water extract; Tmsbw: Terminalia mantaly stem bark water extract; Tslw: Terminalia superba leaf water extract; Tsrm: Terminalia superba root methanol extract; H: hexane; Chl: chloroform; EA: ethyl acetate; R3: final residue)
Overall, 11 fractions showed antiplasmodial potential against both resistant and sensitive strains of P. falciparum, with IC50 values ranging from 0.36 to 14.09 µg/mL. Four out of those fractions codified TmlwChl, TmsbwChl, TmsbwEA, and TsrmEA exhibited highly potent antiplasmodial activity with IC50 values ≤ 2 µg/mL on both strains, and high selectivity (SI > 109).
The chloroform fraction (TmlwChl) from the leaf aqueous extract of Terminalia mantaly (Tmlw) showed the highest antiplasmodial activity, with IC50PfINDO = 0.36 µg/mL and IC50Pf3D7 = 0.96 µg/mL. Two fractions from the stem bark aqueous extract of Terminalia mantaly (Tmsbw) exhibited high activity mainly against the resistant PfINDO strain, namely the chloroform fraction (TmsbwChl, IC50PfINDO: 0.56 µg/mL against IC50Pf3D7: 1.12 µg/mL), and the ethyl acetate fraction (TmsbwEA, IC50PfINDO: 0.68 µg/mL against IC50Pf3D7: 1.35 µg/mL). These two fractions were found to be about twofold more active against the resistant PfINDO strain than the sensitive Pf3D7, and also showed very good selectivity with SI > 148. The ethyl acetate fraction (TsrmEA) of the root methanolic extract of Terminalia superba was also quite promising (IC50PfINDO: 1.82 µg/mL, IC50Pf3D7: 1.65 µg/mL, SI > 109). Three other fractions from both plants (Terminalia mantaly and Terminalia superba) showed significant activity profiles with IC50 values ranging (2.26–4.93 µg/mL), viz. TmsbwH (IC50PfINDO: 4.50, IC50Pf3D7: 4.40 µg/mL), TmsbwR3 (IC50PfINDO: 2.80, IC50Pf3D7: 2.44 µg/mL), and TsrmChl (IC50PfINDO: 2.26, IC50Pf3D7: 4.93 µg/mL). However, these latter fractions showed moderate selectivity (8 < SI > 71) compared to the more potent ones.
Crude extract fractionation led to fractions and final residues with varied activities against P. falciparum parasites. The overall more potent fraction TmlwChl (IC50PfINDO: 0.36 µg/mL and IC50Pf3D7: 0.96 µg/mL; SI > 208) with an average 4.3-fold activity magnification derived from the crude extract Tmlw (IC50PfINDO: 2.09 µg/mL and IC50Pf3D7: 2.66 µg/mL; SI > 75). Fractionation of Tsrm led to no significant change in activity. Fractionation of Tmsbw (IC50PfINDO: 0.26 µg/mL and IC50Pf3D7: 1.03 µg/mL; SI > 194) slightly reduced the antiplasmodial potency (but led to promising and selective fractions—IC50PfINDO: 0.39–4.50 µg/mL and IC50Pf3D7: 1.12–4.40 µg/mL), whereas fractionation of extract Tslw (IC50PfINDO: 0.57 µg/mL and IC50Pf3D7: 1.26 µg/mL) negatively impacted its potency leading to only two moderately active fractions (TslwH and TslwEA-IC50: 6.89–14.09 µg/mL).
The thresholds for the in vitro antiplasmodial activity of the plant extracts/fractions were based on the classification according to Gessler et al. [15] where the promise of the extract is based on its potency: IC50 < 10 µg/mL (very good); 10–50 µg/mL (moderate) and > 50 µg/mL (low activity). Based on this classification, results from this study indicate that most of the tested extracts and fractions exerted very good activities against both sensitive and resistant strains of P. falciparum.
Very few reports are available in the literature on the activity of the studied plants against P. falciparum. Among those, Ngemenya et al. [16] previously reported the antiplasmodial activity (IC50 of 19.5 µg/mL) of methanolic extract from the leaf of Terminalia superba against the chloroquine-sensitive P. falciparum F32 strain. This value is about five to ninefold greater than that of similar extract tested in this study against PfINDO and Pf3D7. This activity discrepancy might be explained by the difference in parasite strains and approaches used and the specific features of parasites relating to drug susceptibility. Indeed, there might be a relationship between in vitro adaptation to culture of P. falciparum and drug-resistant characteristic of the strain. There is also the possibility of the emergence of a drug-resistant sub-population or of changes in the metabolic pathways during the course of in vitro routine culture maintenance [17]. Likewise, Adewunmi et al. [18] investigated the activity of root and stem of Terminalia superba against Trypanosoma congolense IL 1180 and reported IC50 values of 56.1 µg/mL (root ethanolic extract), 91.73 and 55.26 µg/mL for stem hexane and ethanolic extracts. These findings further highlight the potential of Terminalia superba as a source of anti-protozoan principles.
The antiplasmodial activities of extracts, fractions and isolated compounds from many Terminalia species have been previously reported. However, this study is reporting for the first time the antiplasmodial activity of extracts from Terminalia mantaly. Moreover, it is the first report on the antiplasmodial activity of Terminalia species against P. falciparum chloroquine-resistant INDO strain.
Muganga et al. [19] reported the antiplasmodial activity of Terminalia mollis crude methanolic extract (IC50: 3.84 µg/mL), aqueous extract (IC50: 4.66 µg/mL), ethyl acetate fraction (IC50: 2.10 µg/mL), aqueous fraction (IC50: 19.72 µg/mL) and isolated ellagic acid (IC50: 0.17 µg/mL) against P. falciparum 3D7 strain. Mohd Abd Razak et al. [20] reported the antiplasmodial activity of Terminalia catappa aqueous extract (IC50: 4.28 µg/mL), methanolic extract (IC50: 5.19 µg/mL) and dichloromethane extract (IC50: 5.29 µg/mL) on P. falciparum K1. Abiodun et al. [21] also reported the antiplasmodial activity of hexane, ethyl acetate and methanolic extracts from Terminalia catappa on P. falciparum K1 (IC50: 10.10, 3.05 and 7.42 µg/mL, respectively) and P. falciparum NF54 (IC50: 21.93, 6.68 and 9.40 µg/mL, respectively). Sanon et al. [22] reported the antiplasmodial activity of Terminalia avicennioides aqueous, methanolic and dichloromethane extracts from leaf and stem bark with IC50 values ranging from 1.60 to 7.40 µg/mL on P. falciparum K1. Ouattara et al. [23] reported the activity of Terminalia avicennioides ethyl acetate and butanol crude extracts against P. falciparum K1, while ellagic acid isolated from the leaf showed potent antiplasmodial activity with an IC50 of 0.52 µM. Bavagan et al. [24] previously reported the antiplasmodial activity of Terminalia chebula hexane, ethyl acetate, acetone, and methanolic extracts on P. falciparum 3D7 with IC50 values of 51.91, 67.45, 4.76, 42.98 µg/mL, respectively. They equally highlighted the antiplasmodial potential of aqueous extracts from stem bark and stem wood of Terminalia spinosa on P. falciparum chloroquine-resistant ENT36 (IC50: 29.50 and 49.20 µg/mL) and chloroquine-sensitive K67 (IC50: 9.90 and 35.90 µg/mL) strains.
The findings in this report, together with all previous data, emphasize the potential of Terminalia species to produce secondary metabolites with potent antiplasmodial activity. Furthermore, recent reports on phytochemical studies of Terminalia mantaly mainly showed the presence of phenols, flavonoids, tannins, saponins, and steroids [25, 26]. Many compounds belonging to these classes of phytochemicals have been found to be highly potent against several sensitive and resistant strains of P. falciparum [4, 19, 27, 28, 29, 30]. Moreover, studies on their potential mechanisms of action revealed that phenolic compounds and derivatives are very active as enzymes inhibitors. Examples of such enzymes inhibited by phenolic compounds and derivatives are aspartic proteases, xanthine oxidase, 1,5-lipoxygenase, α-glucosidase, glucose-6-phosphate dehydrogenase, carbonic anhydrase and glutathione-S-transferase [26, 30, 31]. It is noteworthy that nowadays some of the abovementioned enzymes including aspartic proteases, glutathione-S-transferase are clearly identified as potential new targets for drug discovery against malaria and several others metabolic dysfunctions of public health significance including cancer, obesity, epilepsy and gout.
Conclusion
Results from this study clearly demonstrate the activity of Terminalia mantaly and Terminalia superba plants extracts against P. falciparum, the causative agent of malaria. To a reasonable extent, they also partly support the traditional uses of these plants in ethno-medicine to treat malaria. However, full validation of this use will depend on the results of detailed toxicological studies of the active extracts. The findings reported here have great scientific significance as they highlight for the first time the antiplasmodial activity of Terminalia mantaly. Identified extracts and fractions with very good antiplasmodial potential and selectivity will be further fractionated following activity-guided approach, and the isolated hit compounds polished and progressed towards novel anti-malarial drugs development.
Notes
Authors’ contributions
FFB and DS designed and supervised the study; CDJM, RMTK, RK, LRYT, PVTF, and BMTT carried out plant extraction, fractionation, in vitro susceptibility testing and drafted the manuscript; PVTF, DS and FFB critically revised the manuscript. All authors read and approved the final manuscript.
Acknowledgements
The authors are grateful to Mr. Victor Nana from the National Herbarium of Cameroon for his assistance in plant collection and identification.
Competing interests
The authors declare that they have no competing interests.
Availability of data and materials
All data used, generated or analysed during the study are included in this research article.
Ethics approval and consent to participate
Not applicable.
Funding
The study was supported by Grant FR: 3240287192 of the ICCBS-TWAS Fellowship to RMTK and the equipment from the Seeding Labs Instrumental Access Grant (SL2012-2) to FFB.
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
- 1.WHO. World malaria report 2015. Geneva: World Health Organization; 2015.Google Scholar
- 2.WHO. World malaria report 2010. Geneva: World Health Organization; 2010.Google Scholar
- 3.Ntonifor NH, Veyufambom S. Assessing the effective use of mosquito nets in the prevention of malaria in some parts of Mezam division, Northwest Region Cameroon. Malar J. 2016;15:390.CrossRefPubMedPubMedCentralGoogle Scholar
- 4.Kamkumo RG, Ngoutane AM, Tchokouaha LRY, Fokou PVT, Madiesse EAK, Legac J, et al. From Sorindeia juglandifolia (Anacardiaceae) exhibit potent antiplasmodial activities in vitro and in vivo. Malar J. 2012;11:382.CrossRefPubMedPubMedCentralGoogle Scholar
- 5.Tsabang N, Tsouh FPV, Yamthe TLR, Noguem B, Bakarnga-Via I, Dongmo NMS, et al. Ethnopharmacological survey of Annonaceae medicinal plants used to treat malaria in four areas of Cameroon. J Ethnopharmacol. 2012;139:171–80.CrossRefPubMedGoogle Scholar
- 6.Dondorp AM, Nostern F, Yi P, Das D, Phyo AP, Tarning J, et al. Artemisinin-resistant Plasmodium falciparum malaria. N Engl J Med. 2009;361:455–67.CrossRefPubMedPubMedCentralGoogle Scholar
- 7.Cheeseman IH, Miller BA, Nair S, Nkhoma S, Tan A, Tan JC, et al. A major genome region underlying artemisinin resistance in malaria. Science. 2012;336:79–82.CrossRefPubMedPubMedCentralGoogle Scholar
- 8.Dobson SMM. The history of antimalarial drugs. In: Rosenthal PJ, editor. Antimalarial chemotherapy: mechanisms of action, resistance, and new directions in drug discovery. Totowa: Humana Press; 2001. p. 15–25.Google Scholar
- 9.Titanji VPK, Zofou D, Ngemenya MN. The antimalarial potential of medicinal plants used for the treatment of malaria in Cameroonian folk medicine. Afr J Trad CAM. 2008;5:302–21.Google Scholar
- 10.Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth. 1983;65:55–63.CrossRefGoogle Scholar
- 11.Trager W, Jensen JB. Human malaria parasites in continuous culture. Science. 1976;193:673–5.CrossRefPubMedGoogle Scholar
- 12.Kaushik NK, Bagavan A, Rahuman AA, Zahir AA, Kamaraj C, Elango G, et al. Evaluation of antiplasmodial activity of medicinal plants from North Indian Buchpora and South Indian Eastern Ghats. Malar J. 2015;14:65.CrossRefPubMedPubMedCentralGoogle Scholar
- 13.Smilkstein M, Sriwilaijaroen N, Kelly JX, Wilairat P, Riscoe M. Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening. Antimicrob Agents Chemother. 2004;48:1803–6.CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Lambros C, Vanderberg JP. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol. 1979;65:418–20.CrossRefPubMedGoogle Scholar
- 15.Gessler MC, Nkunya MHN, Nwasumbi LB, Heinrich M, Tonner M. Screening Tanzanian medical plants for antimalarial activity. Acta Trop. 1994;55:65–7.CrossRefGoogle Scholar
- 16.Ngemenya MN, Tane P, Berzins K, Titanji VPK. Antiplasmodial activity of some medicinal plants used in Cameroon: preliminary toxicity studies of highly active extracts. In: XIth annual conference of the Cameroon Bioscience Society, 16–18 December, 2004.Google Scholar
- 17.Le Bras J, Deloron P, Ricour A, Andrieu B, Savel J, Coulaud JP. Plasmodium falciparum: drug sensitivity in vitro of isolates before and after adaptation to continuous culture. Exp Parasitol. 1983;56:9–14.CrossRefPubMedGoogle Scholar
- 18.Adewunmi CO, Agbedahunsi JM, Adebajo AC, Aladesanmi AJ, Murphy N, Wando J. Ethno-veterinary medicine: screening of Nigerian medicinal plants for trypanocidal properties. J Ethnopharmacol. 2001;77:19–24.CrossRefPubMedGoogle Scholar
- 19.Muganga R, Angenot L, Tits M, Frédérich M. In vitro and In vivo antiplasmodial activity of three Rwandan medicinal plants and identification of their active compounds. Planta Med. 2014;80:482–9.CrossRefPubMedGoogle Scholar
- 20.Mohd Abd Razak MR, Afzan A, Ali R, Jalaluddin NFA, Wasiman MI, Zahari SHS, et al. Effect of selected local medicinal plants on the asexual blood stage of chloroquine resistant Plasmodium falciparum. BMC Complement Altern Med. 2014;14:492.CrossRefPubMedPubMedCentralGoogle Scholar
- 21.Abiodun O, Gbotosho G, Ajaiyeoba E, Happi T, Falade M, Wittlin S, et al. In vitro antiplasmodial activity and toxicity assessment of some plants from Nigerian ethnomedicine. Pharm Biol. 2011;49:9–14.CrossRefPubMedGoogle Scholar
- 22.Sanon S, Gansane A, Ouattara LP, Traore A, Ouedraogo IN, Tiono A, et al. In vitro antiplasmodial and cytotoxic properties of some medicinal plants from western Burkina Faso. Afr J Lab Med. 2013;2:1–7.CrossRefGoogle Scholar
- 23.Ouattara LP, Sanon S, Mahiou-Leddet V, Gansané A, Baghdikian B, Traoré A, et al. In vitro antiplasmodial activity of some medicinal plants of Burkina Faso. Parasitol Res. 2014;113:405–16.CrossRefPubMedGoogle Scholar
- 24.Bagavan A, Rahuman AA, Kamaraj C, Kaushik NK, Mohanakrishnan D, Sahal D. Antiplasmodial activity of botanical extracts against Plasmodium falciparum. Parasitol Res. 2011;108:1099–109.CrossRefPubMedGoogle Scholar
- 25.Ngouana TK, Mbouna CDJ, Kuipou RMT, Tchuenmogne MAT, Zeuko’o EM, Ngouana V, et al. Potent and synergistic extract combinations from Terminalia catappa, Terminalia mantaly and Monodora tenuifolia against pathogenic yeasts. Medicines (Basel). 2015;2:220–35.CrossRefGoogle Scholar
- 26.Tchuenmogne MAT, Kammalac TN, Gohlke S, Kouipou RMT, Aslan A, Kuzu M, et al. Compounds from Terminalia mantaly L. (Combretaceae) stem bark exhibit potent inhibition against some pathogenic yeasts and enzymes of metabolic significance. Medicines (Basel). 2017;4:6.CrossRefGoogle Scholar
- 27.Zofou D, Kowa TK, Wabo HK, Ngemenya MN, Tane P, Titanji VPK. Hypericum lanceolatum (Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark. Malar J. 2011;10:167.CrossRefPubMedPubMedCentralGoogle Scholar
- 28.Pham AT, Nguyen C, Malterud KE, Diallo D, Wangensteen H. Bioactive flavone-C-glycosides of the African medicinal plant Biophytumum braculum. Molecules. 2013;18:10312–9.CrossRefPubMedGoogle Scholar
- 29.Su Q, Krai P, Goetz M, Cassera MB, Kingston DGI. Antiplasmodial isoflavanes and pterocarpans from Apoplanesia paniculata. Planta Med. 2015;81:1128–32.CrossRefPubMedPubMedCentralGoogle Scholar
- 30.Malterud KE. Ethnopharmacology, chemistry and biological properties of four Malian medicinal plants. Plants (Basel). 2017;6:11.CrossRefGoogle Scholar
- 31.Meyersa MJ, Andersona EJ, McNitta SA, Krenninga TM, Singha M, Xub J, et al. Evaluation of spiropiperidine hydantoins as a novel class of antimalarial agents. Bioorg Med Chem. 2015;23:5144–50.CrossRefGoogle Scholar
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