Skip to main content

Table 1 Primer sets and PCR conditions for DNA amplification of Plasmodium species

From: Malaria in children of Tshimbulu (Western Kasai, Democratic Republic of the Congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting

Plasmodium species Primers Product size (bp) PCR conditions
Plasmodium spp. PgMt19F3 5′-TCG CTT CTA ACG GTG AAC-3′
PgMt19B3 5′-AAT TGA TAG TAT CAG CTA TCC ATA G-3′
250 95 °C, 15′; 5 cycles [95 °C 10″, 62 °C 30″, 72 °C 15″]; 45 cycles [95 °C 10″, 62 °C 10″, 72 °C 15″] 72 °C, 10′
P. falciparum forward 5′-AAC AGA CGG GTA GTC ATG ATT GAG-3′ 276 95 °C, 15′; 43 cycles [95 °C 45″, 60 °C 90″, 72 °C 45″]; 72 °C, 10′
P. malariae forward 5′-CGT TAA GAA TAA ACG CCA AGC G-3′ 412
P. ovale forward 5′-CTG TTC TTT GCA TTC CTT ATG C-3′ 375
P. vivax forward 5′-CGG CTT GGA AGT CCT TGT-3′ 300
Species-specific reverse 5′-GTA TCT GAT CGT CTT CAC TCC C-3′