Malaria in children of Tshimbulu (Western Kasai, Democratic Republic of the Congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting

Table 1 Primer sets and PCR conditions for DNA amplification of Plasmodium species

Plasmodium species	Primers	Product size (bp)	PCR conditions
Plasmodium spp.	PgMt19F3 5′-TCG CTT CTA ACG GTG AAC-3′ PgMt19B3 5′-AAT TGA TAG TAT CAG CTA TCC ATA G-3′	250	95 °C, 15′; 5 cycles [95 °C 10″, 62 °C 30″, 72 °C 15″]; 45 cycles [95 °C 10″, 62 °C 10″, 72 °C 15″] 72 °C, 10′
P. falciparum forward	5′-AAC AGA CGG GTA GTC ATG ATT GAG-3′	276	95 °C, 15′; 43 cycles [95 °C 45″, 60 °C 90″, 72 °C 45″]; 72 °C, 10′
P. malariae forward	5′-CGT TAA GAA TAA ACG CCA AGC G-3′	412
P. ovale forward	5′-CTG TTC TTT GCA TTC CTT ATG C-3′	375
P. vivax forward	5′-CGG CTT GGA AGT CCT TGT-3′	300
Species-specific reverse	5′-GTA TCT GAT CGT CTT CAC TCC C-3′