Fig. 6
From: Bronchoscopic biopsies - a novel source for primary airway epithelial cells in respiratory research
Influence of house dust mite extract (HDM) on differentiation of airway epithelial cells (AECs). After 3 weeks in ALI, AECs from conventional, forceps, and cryo-sampled cells were untreated or stimulated with HDM for 6–24 h (n = 4–5). (A) Immunofluorescence staining of goblet cells (green–MUC5AC; blue–Hoechst, red–ZO1) (n = 5; scale bar 40 μm); representative pictures are shown. (B) Numbers by amount of MUC5AC+ goblet cell or ac. α-tubulin+ cells were determined per cm2 (n = 4–5). (C). Analyses of goblet cells (TSPAN8+) and club cells (CD66a/c/e+) by flow cytometry (n = 5). (B) + (C) Results are expressed as mean ± standard deviation (plot: min to max, show all points). Statistical analyses were performed by two-way analysis of variance with Tukey’s post-hoc test, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001