Fig. 5
From: Bronchoscopic biopsies - a novel source for primary airway epithelial cells in respiratory research
Differentiation of airway epithelial cells (AECs) in air-liquid interface (ALI). Cellular composition of cultivated AECs on day 8, at air exposure, and after 1, 2, or 3 weeks in ALI was analyzed by flow cytometry (n = 5) using the following markers for each epithelial cell type (CD326+): (A) percentage of basal (CD49f+ CD271+), (B) club (CD66a/c/e+), (C) goblet (TSPAN8+). Results are expressed as mean ± standard deviation (plot: min to max, show all points). Statistical analyses were performed by two-way analysis of variance with Tukey’s post-hoc test