Fig. 4
From: Bronchoscopic biopsies - a novel source for primary airway epithelial cells in respiratory research
Differentiation of airway epithelial cells (AECs) in air-liquid interface (ALI). (A) Ciliation was analyzed by scanning electron microscopy after 3 weeks in ALI using conventional, forceps, and cryo-sampled AECs (n = 5); representative pictures are shown; scale bar 10 μm. (B) Representative pictures of ciliation were analyzed by immunofluorescence staining (green–ac. α-tubulin; blue–Hoechst, red–ZO1) after 3 weeks in ALI using conventional, forceps, and cryo-sampled AECs (n = 5; scale bar 40 μm). (C) Differentiation of ALI-AECs after 3 weeks using conventional, forceps, and cryo-sampled cells analyzed by periodic acid-Schiff reaction (PAS, n = 5); goblet cells are marked by an arrow; representative pictures are shown; scale bar 50 μm