Fig. 2
From: Bronchoscopic biopsies - a novel source for primary airway epithelial cells in respiratory research
Sampling of primary airway epithelial cells (AECs): vitality, yield, and purity. (A) Sampling method and tissue size of the conventional method or by forceps and cryo biopsy. In the conventional method, AECs were scraped off the mucosal surface after enzymatic digestion. Forceps and cryo biopsy were taken tangentially followed by enzymatic digestion. Representative pictures of tissues sizes used for cell isolation are shown for each sampling procedure. (B) Vitality directly after scraping or biopsy. Results are shown as box and whiskers (plot: min to max, show all points, n = 4). (C) Cell yield (day 8) of each sampling method. Results are shown as box and whiskers (plot: min to max, show all points, conventional: n = 11, forceps: n = 4, cryo: n = 4). (D) Flow cytometry was performed to determine cellular composition using the following markers for each cell type of AECs (CD326+): percentage of basal (CD49f+CD271+), club (CD66a/c/e+), goblet (TSPAN8+), and ciliated cells (ac. α-tubulin+). Results are shown as mean ± standard deviation. All results were analyzed by two-way analysis of variance with Tukey’s post-hoc test