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Table 2 Characterization of the DNA inserts from the resistant clones found in the oral metagenomic library

From: Functional screening of a human saliva metagenomic DNA reveal novel resistance genes against sodium hypochlorite and chlorhexidine

Sample name (Accession number) Size (bp) BlastN BlastX
Closest homologue Percentage identity (%) Coverage (%) Accession number of the homologous DNA (BlastN) Closest homologue ORF size (bp) Percentage identity (%) Coverage (%) Position on sample Accession number of the homologous proteins (BlastX)
Sodium hypochlorite- 1 (MZ955857) 12,588 Rothia mucilaginosa strain FDAARGOS_369 chromosome, complete genome 90.32 77 CP023510.1 Helix-turn-helix transcriptional regulator [Rothia mucilaginosa] 168 100 41 2 → 169 WP_049346502.1
MULTISPECIES: DUF3046 domain-containing protein [Rothia] 222 100 100 347 → 568 WP_049333114.1
MULTISPECIES: Recominase RecA [Rothia] 1107 99 100 979 → 2085 WP_049332756.1
RecX family trancriptional regulator [Rothia sp. HMSC072E10] 1938 97 100 2292 → 4229 WP_070680682.1
Hemin ABC transporter substrate-binding protein [Rothia mucilaginosa] 1476 98 100 4391 ← 5866 WP_152901671.1
MULTISPECIES: tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB [Rothia] 1515 100 100 5883 → 7397 WP_049332750.1
tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA [Rothia mucilaginosa] 930 99 100 7474 → 8403 WP_193389320.1
MULTISPECIES: Diaminopimelate epimerase [Rothia] 936 100 100 8501 → 9436 WP_049344416.1
MULTISPECIES: Methyltransferase [Rothia] 624 100 100 9603 ← 10,226 WP_049332745.1
GTPase HflX (Rothia sp. HMSC068F09) 1581 99 94.3 10,426 → 12,006 WP_070648960.1
ATP-dependent DNA helicase [Rothia sp. HMSC072B03] 472 96 22.6 12,117 → 12,588 WP_070482790.1
Chlorhexidine- 1 (MZ955858) 1015 No significant similarity found Type IV secretion system DNA-binding domain-containing protein [Kocuria indica] 237 51 10.8 746 → 982 WP_121544318.1
Chlorhexidine- 2 (MZ955859) 1536 Streptococcus parasanguinis ATCC 15,912, complete genome 99.28 92 CP002843.1 MULTISPECIES: Beta-ketoacyl-ACP synthase II [Streptococcus] 659 100 53.2 1 → 659 WP_014712692.1
MULTISPECIES: Acetyl-CoA carboxylase biotin carboxyl carrier protein [Streptococcus] 489 100 100 663 → 1151 WP_061604604.1
MULTISPECIES: 3-hydroxyacyl-ACP dehydratase FabZ [Bacteria] 267 99 62.4 1270 → 1536 WP_003004019.1
Erythromycin-1 (MZ955860) 5528 Streptococcus mitis strain SK637 chromosome, complete genome 99.74 96 CP028415.1 Hypothetical protein [Streptococcus mitis] 432 100 99.3 223 → 654 CDG57854.1
MULTISPECIES: Hypothetical protein [Bacteria] 336 97 100 651 → 986 WP_000806926.1
YolD-like protein [Streptococcus pneumoniae] 369 99 100 998 → 1366 VLH51388.1
MULTISPECIES: Hypothetical protein [Bacteria] 300 100 100 1354 → 1654 WP_001072467.1
ABC-F type ribosomal protection protein Msr(D) [Streptococcus pneumoniae] 2064 99 100 1771 ← 3234 WP_000420316.1
Macrolide-efflux protein [Streptococcus pneumoniae] 1194 98 93 3379 ← 4572 VQI18798.1
Hypothetical protein [Streptococcus pneumoniae] 186 100 100 4960 ← 5145 AAR22392.1
Triclosan-1 (MZ95561) 5408 Streptococcus sp. oral taxon 431, complete genome 91.83 99 CP014264.1 Enoyl-[acyl-carrier-protein] reductase FabK [Streptococcus sp. SK643] 1020 96 100 629 → 1648 WP_196793360.1
ACP S-malonyltransferase [Streptococcus sp. HMSC034E03] 921 98 100 1641 → 2561 WP_075232029.1
3-oxoacyl-[acyl-carrier-protein] reductase [Streptococcus sp. HMSC034E03] 735 99 100 2595 → 3329 WP_075232030.1
Beta-ketoacyl-ACP synthase II [Streptococcus mitis] 1236 99 100 3352 → 4587 WP_075232031.1
MULTISPECIES: 3-hydroxyacyl-ACP dehydratase FabZ [Streptococcus] 384 100 91.4 5025 → 5408 WP_042901453.1
Triclosan-2 (MZ955862) 3944 Streptococcus salivarius strain NCTC8618 genome assembly, chromosome: 1 99.75 97 LR134274.1 MULTISPECIES: Enoyl-CoA hydratase [Streptococcus] 389 100 49 1 → 389 WP_037601410.1
MULTISPECIES: Ketoacyl-ACP synthase III [Streptococcus] 963 99 100 926 → 1888 WP_037612035.1
MULTISPECIES: Enoyl-[acyl-carrier-protein] reductase FabK [Streptococcus] 966 100 100 2283 → 3248 WP_022495984.1
ACP S-malonyltransferase [Streptococcus salivarius] 627 100 73.2 3318 → 3944 WP_049545679.1
Tetracycline-1 (MZ955863) 6366 Streptococcus australis strain NCTC13166 genome assembly, chromosome: 1 93.08 99 LS483444.1 Tetracycline efflux ABC transporter Tet(46) subunit A [Streptococcus parasanguinis] 1725 99 97.5 380 → 2104 WP_125826751.1
Tetracycline efflux ABC transporter Tet(46) subunit B [Streptococcus parasanguinis] 1737 99 100 2106 → 3842 WP_049497012.1
MULTISPECIES: rRNA maturation RNase YbeY [Streptococcus] 498 99 100 3939 → 4436 WP_003005431.1
MULTISPECIES: GTPase Era [Streptococcus] 900 99 100 4845 → 5744 WP_003004993.1
MULTISPECIES: DNA-formamidopyrimidine glycosylase [Streptococcus] 577 98 67 5790 → 6366 WP_070587080.1