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Table 1 Effect of PBM on cancer cells, in vitro and in vivo experiments between 630 and 670 nm

From: Mechanisms of PhotoBioModulation (PBM) focused on oral mucositis prevention and treatment: a scoping review

  PBM parameters Cell Effect References
  Light source Wave length nm Dose (J/cm2)
In vitro GaAlAs 660 2.05; 3.07; 6.15 Oral dysplastic cells (DOK); oral cancer cells (SCC-9 and SCC-25) Modify cell growth signaling pathway; aggravate oral cancer cellular behavior [16]
Sperandio; 2013
Diode Laser 660 0.5; 1 SCC-25 cells Stimulatory effect on cell proliferation and invasion [17]
Gomes Henriques;2014
GaAlAs laser 660 0.39 to 63.7 mW/cm2 SCC-25 cells LLLT induced a significant increase in the percentage of S-phase associated with a decrease in SCC-25 cells proliferation [18]
Schartinger; 2012
Diode laser (LTIAO00-PLT20 636 Between 5 and 20 Lung cancer stem cells LILI induced biostimulatory effects [44]
GaAlAs laser 660 30, 90, 150 J/cm2 Fibroblasts and tumor cells submitted to IR with doses of 2.5 Gy and 10 Gy No influence of LLLT on tumor cell viability; decrease in proliferation and increase in senescence for tumor cells [21]
Ramos Silva; 2016
laser 635–670 Between 0.04 and 4.8 H.Ep.2 cells (malignant cells) LLLT with 670 nm could significantly increase proliferation of laryngeal cancer cells. Whereas 635 nm wavelength does not stimulate significantly cells proliferation [45]
In vivo HF-LPLI laser 633 120 for in vitro experiments
1 200 for in vivo experiments (one time only)
Mice with tumor cells Cancer cell apoptosis, antitumor immunity [20]
Lu; 2015
Diode Laser 650 0, 15, 30
1 treatment during 150 or 300 s
Anaplastic thyroid cancer cell line FRO injected into thyroid glands of nude mice Overproliferation and angiogenesis of anaplastic thyroid carcinoma [19]
Rhee; 2016
Diode Laser 633 3.5 three times a week for 2 weeks Model of human gastric adenocarcinoma transplanted into immunodeficient athymic nude mice Acceleration tumor growth in conditions without immune resistance [46]
Thesis; 2010
Diode Laser 660 56.4
Every other day for 4 weeks
Squamous cell carcinomas in the hamster Progression of the severity [46]
Red light
670 2.5 per session
Twice a day for 37 days
Mice No harmful effect of whole-body red LLLT on tumor growth [47]
Myakishev-Rempel; 2012
In vitro, in vivo CW semiconductor laser 635 134 one time ASTC-a-1 and A549 cells in mice HF-LPLI can selectively photoinactivate respiratory chain oxidase producing oxidative damage on cancer cells [48]
Wu; 2014
Laser source 660 150 or 1050 once a day for three days B16F10 melanoma cells Cancer-protective effect in vitro but not reproduced in the in vivo experiment [49]
Frigo; 2009
  1. Cell lines (DOK cell line: Dysplastic oral keratinocytes; SCC-9 and SCC-25: oral squamous cell carcinoma cell lines; H.Ep.2 cells (SCC type 2) originate from laryngeal carcinomas, Cell line FRO: human anaplastic thyroid cells, ASTC a 1/A549: human lung adenocarcinoma cell line, B16F10: murine melanoma cells)
  2. LLLT: low level laser therapy, LILI: low-intensity laser irradiation, IR: ionizing radiation CW: continuous wave, HF-LPLI: high fluence low-power laser irradiation