Fig. 6

a Predicted secondary structure of LysUE1 with three beta sheets and 8 helixes. b 3D predicted structure viaAlphaFold3, the N-terminal (green) amphipathic helix (amino acid resides 11–20) and C-terminal (red) amphipathic helix (amino acid resides 49–60). c SDS‒PAGE analysis of LysUE1 using NiNTA column. Lane 1, Marker; Lane 2, tertiary eluate; Lane 3, primary wash effluent; Lane 4 secondary wash effluent; Lane 5, tertiary wash effluent; Lane 6, crude lysate; Lane 7, column flowthrough