Breast cancer stem cells (CSCs) are increasingly thought to play a major role in breast cancer growth and the formation of metastases. CSCs have hierarchical potential to undergo self-renewal along with yielding daughter cells that result in the formation of bulk tumor cells, while maintaining a self-replicating potential [1]. CSCs appear to make up a small minority of most tumors, while in others (for example, melanoma) they may comprise up to 25% of the total mass [2].

Epithelial-to-mesenchymal transition (EMT), a process first noted during embryogenesis, guides the transformation of non-mobile epithelial-like cells into mobile, mesenchymal-like cells that have the potential to travel to distant anatomical sites within the developing embryo (Figure 1). This naturally occurring process has also been observed during tumor formation, and may lead to the development of metastatic growth. The process can be reversed through mesenchymal-to-epithelial transition (MET), where migratory cells become anchored at distant sites and lose their migratory potential.

Figure 1
figure 1

Epithelial-to-mesenchymal transition, mesenchymal-to-epithelial transition, and the migration of cancer stem cells. In the presence of stimulatory signaling (that is, Hedgehog (Hh), Notch, Wnt, transforming growth factor (TGF)-β) primary tumor cells may undergo epithelial-to-mesenchymal transition (EMT), a process where cells suppress E-cadherin expression and lose their tight membrane junctions. Cells can acquire a mobile phenotype and migrate into the circulatory system by entering capillary beds. Exiting the circulatory system at a distant anatomical site, cells undergo the reverse process of mesenchymal-to-epithelial transition (MET), reacquiring their original non-mobile epithelial-like phenotype.

Embryonic signaling pathways, such as the Notch, Hedgehog (Hh), Wnt, and transforming growth factor (TGF)-β pathways, are essential for stem cell signaling during embryogenesis [3]. These pathways play critical roles in normal tissue development and maintenance, and are also involved in the tight regulation of EMT. Deregulation of embryonic signaling pathways has been widely reported in human cancers, including breast, pancreatic, and lung [46]. This observation has led to the evaluation of these pathways as potential targets for a new generation of anti-cancer drugs.

This review examines current findings and perspectives on the interplay between CSCs, embryonic signaling pathways, and EMT/MET in breast tumor growth and metastasis.

Breast cancer stem cells

The cell-of-origin for breast CSCs has yet to be determined, but may be the result of malignant transformation of normal stem/progenitor cells [7]. The long life span of stem/progenitor cells makes them more susceptible to the accumulation of DNA mutations. The capacity to replicate and produce multiple progeny also makes stem and progenitor cells likely candidates for tumor cells-of-origin [8].

BRCA1 is known to play a role in the repair of double-stranded DNA breaks in breast tissue, thereby maintaining chromosomal stability and structure [9]. BRCA1 expression is required for the differentiation of estrogen receptor (ER-) stem/progenitor cells into ER+ luminal cells. Loss of the double-stranded DNA break repair function, seen in BRCA1 deficient or mutant cells, may contribute to the accumulation of genetically unstable breast stem cells, providing a source of cells suitable for carcinogenesis and CSC development [10].

Breast CSCs capable of forming mammospheres were isolated from pleural effusions from breast cancer patients, and were tumorigenic when transplanted into SCID mice [11]. Breast CSCs expressed CD44, but had low or undetectable levels of CD24 and were lineage negative (CD44+CD24-/low/lin-) by flow cytometry [12]. NOD/SCID mice injected with as few as 200 ESA+ CD44+ CD24-/low cells formed tumors that could be serially passaged in vivo. More recently, additional markers, including aldehyde dehydrogenase (ALDH)1, CD133 (prominin-1) [13], CD49f hi and ITGA6 [14], have been proposed as breast CSC biomarkers. ALDH1, a detoxifying enzyme that oxidizes intracellular aldehydes, is found in both normal mammary stem cells and breast cancer stem cell populations [15]. As few as 500 ALDH1+ breast tumor cells were capable of forming tumors when transplanted into NOD/SCID mice. An examination of ALDH expression in human breast cancer found that ALDH1+ tumors were associated with HER2 expression, and the absence of ER and progesterone receptor expression (HER2+, ER-, PR-). ALDH-1 expression in basal-type breast cell lines, but not most luminal cell lines, has been associated with a poor prognosis and decreased overall survival [16].

Significantly, these biomarkers are not expressed universally across all types of breast cancer CSCs, but rather are expressed differentially according to subtype [13, 16]. In addition to histologic phenotype, biomarker expression may also be dependent upon the tumor micro-environment [17]. For example, the microenvironment may provide regulatory signals that modulate the activity of transcription factors and subsequent gene expression. The CSC microenvironment may also provide essential factors that help regulate EMT and MET processes and expression of resulting cellular phenotypes.

Treatment-resistant CD44+/CD24-/lowcells

The enrichment of CD44+/CD24-/low cells in primary breast tumors following chemotherapy suggests an innate resistance to standard treatments [18]. The presence of ATP-binding cassette (ABC) transporters, including ABCG2, first cloned by Doyle and colleagues, is highly expressed in a subpopulation (that is, side population) of CSCs [19, 20]. ABC transporters act to confer resistance to chemotherapeutic agents by extruding these compounds out of the cell [21].

Similarly, enrichment of CD44+/CD24-/low cells has been demonstrated following treatment with radiation therapy [22]. These cells were capable of reproducing in an in vivo model following at least four generations of xenograft transplanted mice [23], suggesting that they play important roles in tumor relapse and metastasis. Potential mechanisms of chemotherapy and radiation resistance in breast cancer include the presence of lower concentrations of reactive oxygen species [24], cell dormancy, efficient DNA repair mechanisms [25], and overexpression of Wnt/β-catenin and Notch signaling [22, 26]. Treatment-resistant CD44+/CD24-/low cells, being potential cells-of-origin for metastatic growth, are currently being evaluated as therapeutic targets.

The stem cell niche may also furnish CSCs with a means to evade chemotherapy through the presence of a protective microenvironment [27]. The niche is a complex microenvironment that is actively involved in stem cell growth. The niche acts to shelter stem cells from apoptotic signals, differentiation stimuli, and environmental insults [28]. Bidirectional signaling occurs between the supporting niche and stem cells, and the possibility exists that dysfunctional signaling by the supporting niche may drive CSC growth. Therefore, targeting the CSC niche with new therapeutic agents may disrupt these cellular communications and potentially interfere with CSC growth.

Cross-talk among embryonic signaling pathways

The Hh, Notch, Wnt, and TGF-β signaling pathways, instead of acting as isolated units, may interact through cross-talk to provide tumor cells with an additional mechanism to evade chemotherapy (Figure 2) [29]. The links between embryonic Hh signaling and EMT are relatively well established, but must also be considered in a context-dependent fashion as extensive evidence of cross-talk between the embryonic signaling pathways also occurs. These interlinked network communications may serve as a natural mechanism to increase cellular diversity to extracellular stimuli during embryogenesis and postnatal life. The identification of cross-talk networks in tumor cells may also allow for more effective design and use of combination antitumor therapies.

Figure 2
figure 2

Cross-talk among embryonic signaling pathways and experimental inhibitors. Potential cross-talk among embryonic signaling pathways (transforming growth factor (TGF)-β, Hedgehog (Hh), Notch, Wnt, and ErbB) is shown. The expansive potential for signaling cross-talk suggests that signaling pathways do not function in isolation, but instead are parts of a complex signaling network. Cross-talk can lead to both enhancing and inhibitory interactions between pathways. The actions of experimental pathway inhibitors are shown in yellow. Positive interactions are shown in green, while negative interactions are shown in red.

The TGF-β signaling pathway is known to interact with the Wnt, Hh, and Notch pathways, as well as the mitogen-activated protein kinase (MAPK) pathway [30]. These interactions are complex, context dependent, and reflective of the summary of all input signals. TGF-β signaling helps regulate a wide spectrum of cellular processes, including proliferation, differentiation, apoptosis, cell migration, and metastasis [31]. Understanding the consequences of TGF-β cross-talk with other signaling pathways during tumorigenesis is key to developing effective therapeutic strategies.

Links between HER2 overexpression and activation of the phosphoinositide 3-kinase (PI3K)/Akt pathway suggests cross-talk between these pathways in breast tissue [32]. Treatment of sensitive HER2+ cell lines in vitro with trastuzumab led to decreased expression of both phospho-HER2 and phospho-Akt. In resistant HER2 + cells, trastuzumab treatment had no measurable effect on either HER2 or Akt phosphorylation.

A Notch binding sequence has been reported within the HER2 promoter and suggests a mechanism for Notch/HER2 cross-talk [33]. HER2 expression was shown to be dependent upon Notch signaling in stem-like breast cells [34]. Inhibition, or silencing of Notch signaling, resulted in reduced HER2 expression by these cells. Thus, combining Notch inhibitors with anti-HER2 agents may yield positive results in HER2+ breast cancer patients.

Also, cross-talk between Notch and the ER has been reported in breast cancer cell lines in vitro [35]. Estrogen was shown to inhibit Notch signaling through a mechanism partly mediated by inhibition of Notch cleavage by γ-secretase. Treatment of ERα+ cell lines with anti-estrogens (for example, estradiol) strongly upregulated Notch signaling and led to cell proliferation. Results indicated that Notch signaling may represent a therapeutic target in ERα- breast cancers. Conversely, combination therapies of Notch inhibitors and anti-estrogens may be effective in ERα+ breast cancers.

Notch signaling

The Notch receptors and their ligands moderate short range cellular communications during proliferation, differentiation, embryogenesis and apoptosis [36]. The Notch receptors consist of non-covalently bound extracellular, transmembrane and intracellular domains. Notch receptors interact with a diverse group of ligands from the Delta (-1, -3 and -4) and Serrate/Jagged families (-1 and -2). Receptor cleavage, with eventual release of the Notch intracellular domain (NICD), occurs through a disintegrin and metalloprotease (ADAM) followed by γ-secretase cleavage. Once released from the membrane receptor, NICD translocates to the nucleus where it interacts with the CSL transcription factor to activate the Notch target genes such as HES, HEY and c-Myc [37]. Currently, γ-secretase and aspartyl protease serve as primary targets for Notch-specific investigational drug design.

Although cell type and context dependent, Notch signaling can lead to the initiation of EMT by activating nuclear factor-κB (NF-κB) or altering TGF-β signaling [38, 39]. The evolutionarily conserved Numb acts to regulate Notch activity by interacting with NICD, leading to NICD polyubiquitination and degradation [40]. Numb-mediated Notch inhibition is disrupted in approximately 50% of breast carcinomas, due to degradation of Numb [41]. A relationship between overexpression of Notch signaling and poor overall survival in breast cancer patients has also been observed [42]. Most recently, Notch signaling was shown to have an important role in breast cancer bone metastasis pathophysiology. Notch signaling requires coordinated interaction with other pathways to induce EMT. During embryogenesis, Notch signaling coordinates Jagged 1, a Notch ligand, and HEY1, a Notch target gene, signaling in a Smad3-dependent fashion [43].

TGF-β signaling

TGF-β signaling also plays a significant role in the process of EMT, embryogenesis, and cancer pathogenesis. Key are the TGF-β-induced transcription factors Snail (SNAI1 and SNAI2/Slug), Twist (basic helix-loop-helix), Six family homeobox (Six1) and ZEB (ZEB1 and ZEB2/SIP1) (Figure 3). In breast CSCs, TGF-β signaling appears to modulate stem cell phenotype and maintain pluripotency [31, 38, 44, 45]. TGF-β can alter tight junction formation in mammary epithelium and induce signaling in a number of embryonic signaling pathways, including Wnt, Notch, and Hh pathways. During EMT, SNAI1 and SNAI2/Slug (Snail) associate with SMAD3 and SMAD4 in the TGF-β signaling pathway to initiate cancer growth and metastasis by inhibiting or suppressing transcription of E-cadherin, occludin and claudin [46, 47].

Figure 3
figure 3

Embryonic pathway signaling leads to induction of epithelial-to-mesenchymal transition. Hedgehog (Hh), Notch, Wnt, and transforming growth factor (TGF)-β signaling can activate epithelial-to-mesenchymal transition (EMT) regulators to induce phenotypic changes through a variety of signaling intermediates. EMT-inducing signals by Snail, Slug, KLF8, Twist, Goosecoid, Foxc1 and -2, or Zeb1 and -2 drive non-mobile epithelial-like cells to acquire more invasive phenotypes. By migrating into the circulatory system, cancer stem cells can translocate to new locations and initiate new metastatic growths. EGF, epidermal growth factor; Fzd, Frizzled; Hh, Hedgehog; HIF, hypoxia-inducible factor; NICD, Notch intracellular domain; Smo, Smoothened; TGF, transforming growth factor.

Hedgehog signaling

The Hh ligands Sonic (SHh), Desert (DHh) and Indian (IHh), mediate embryogenesis by defining polarity, morphogenesis, proliferation and differentiation [48]. Hh signaling modulates tissue polarity and is responsible for stem cell maintenance. The Hh ligands bind to a 12-pass transmembrane protein, Patched (PTCH) [49], and binding results in the de-repression of Smoothened (SMO) [50]. SMO then translocates to the primary cilium, which is then internalized and activated. Signaling then proceeds to activate the zinc-finger transcription factors GLI-1, -2 and -3, leading to transcription of GLI target genes. The balance between the GLI-1 and -2 activators and the GLI-2 and -3 repressor forms moderates Hh signaling [51].

EMT and metastases are blocked by SMO antagonists/inhibitors in cell lines from pancreatic cancer [52, 53]. For example, cyclopamine, a SMO antagonist, diminished motility and growth of cells [54, 55]. Hh signaling plays a pivotal role in EMT by inducing the expression of a repressor of E-cadherin, SNAIL1 [56, 57]. High GLI1 is seen in colorectal xenografts with high metastatic potential, epithelial morphology and EMT-associated markers [58]. In this model E-CADHERIN was also suppressed. While evaluating CSCs in the immunocompromised SCID mouse model is useful, it presents limitations for evaluating the role of the immune system, and in particular cytokines, in tumorigenesis and the development of metastases.

Wnt/β-catenin signaling

The Wnt signaling pathway controls development of the mammary gland during embryogenesis and growth and differentiation of the breast during pregnancy and lactation [59]. Currently there are 19 known Wnt genes, 10 Frizzled (Fzd) receptors, and 2 low density lipoprotein receptor-related protein (LRP) co-receptors [60].

In the inactive state, cytoplasmic β-catenin is depleted from the cell via the destruction complex consisting of adenomatous polyposis coli (APC), glycogen synthase kinase 3β (GSK3β), Axin, and casein kinase-1α [61]. During Wnt signaling, activity of the destruction complex is inhibited, allowing accumulation and translocation of β-catenin to the nucleus, where it interacts with the lymphoid enhancer factor/T cell factor (Lef/Tcf) complex leading to targeted gene transcription.

Wnt signaling can be broadly grouped as either canonical/β-catenin or non-canonical. Canonical signaling, the result of Wnt binding to Fzd, leads to β-catenin accumulation within the cell nucleus. β-catenin accumulation within the nucleus or cytoplasm has been reported in approximately 50% of breast carcinomas and has been correlated with poor prognosis [62]. Non-canonical Wnt signaling does not result in β-catenin accumulation in the nucleus.

Tumor metastasis

The dissemination of cancer cells may occur at an early stage of malignancy, but tumor cell dormancy, a time lag between tissue infiltration and tumor formation, may result in metastatic latency [63, 64]. Understanding the sequential stepwise events during metastasis is essential for the development of novel therapeutic agents. However, considering the differences in target organs and rates of metastatic growth, optimizing therapeutic interventions remains challenging.

The relationship between the niche microenvironment and metastasis was first demonstrated by the characterization of bone marrow-derived cells that were directed to and colonized the pre-metastatic niche [65]. The recruitment of bone marrow-derived cells to the pre-metastatic niche may involve a variety of secreted factors, including osteopontin [66]. Weakly metastatic human breast cancer cells could be induced to form metastatic tumors in mice when mixed with bone marrow-derived mesenchymal stem cells [67]. Induction of tumor formation was reversible and dependent on chemokine CCL5 (RANTES) signaling through the CCR5 receptor. These studies suggest a link between cytokine concentrations in the stem cell microenvironment and induction of meta-static tumor growth.

Gene expression studies performed on human breast cancer specimens have identified a breast cancer molecular subtype 'claudin-low' population [68] that expresses an overlapping gene signature with that of CSC populations enriched with CD44+/CD24-/low cells using fluorescence-activated cell sorting (FACS), and mammospheres [69]. Claudin-low breast tumors are typically 'triple negative' invasive, ductal carcinomas associated with a poor prognosis. Using genetically modified cell lines, the 'claudin-low' population expressed low levels of Claudin 3 and E-cadherin and also expressed EMT markers such as vimentin and Twist, suggesting that claudin-low cells may have derived from immature progenitor or stem cells [70]. An EMT gene expression signature in human mammary epithelial cells, produced by overexpressing either Goosecoid (Gsc), Snail, Twist, or TGF-β1, or by knocking down expression of E-cadherin, was found to be most similar to the gene expression signature found in claudin-low and meta-plastic breast cancers. The EMT signature most closely resembled the gene signature of basal B cell lines, characterized by high vimentin expression and a cancer stem cell-like profile.

Epithelial-to-mesenchymal transition

Deregulation of EMT, an essential process during embryogenesis and adult tissue repair and maintenance, may result in the mobilization and spread of primary tumor cells to distant locations. When adherent epithelial cells acquire mesenchymal properties, gene-expression patterns change and the cells acquire an increased motility potential. In the case of cancer, EMT and MET may serve to regulate cellular plasticity and play important roles during tumor invasion, metastasis, and therapeutic resistance [71].

EMT is also linked with the acquisition of stem cell characteristics [44]. The concept of CSCs conferring both EMT and self-renewal properties provides the rationale for cancer cells to migrate and populate metastatic sites. It also provides the rationale for targeting the CSC population with anti-tumor therapies to inhibit metastasis.

Regulators of epithelial-to-mesenchymal transition

The functional loss of E-cadherin is the most critical event linked with EMT. The loss of these membrane adhesive proteins allows previously stationary tumor cells to dislodge from their original location and become more mobile. E-cadherin expression is controlled by various mechanisms, including inactivating mutations [72], epigenetic modulation through promoter hypermethylation [73], and transcriptional repression by zinc-finger transcription factors with high affinity for the E-box elements of E-cadherin promoter [74, 75]. At the metastatic site, in the absence of additional EMT modulators in the stromal microenvironment, the cell transforms back to the original epithelial phenotype (via MET) [76]. This important concept suggests that transcription of the E-cadherin gene, or post-translational modification, may be partly controlled by epigenetic mechanisms.

Epigenetic and DNA mutations

Hypermethylation and silencing of the E-cadherin promoter is known to cause transcriptional down-regulation of the E-cadherin gene and is linked to the initiation of EMT, migration and invasion in breast cancer [73, 77]. Reversibility of E-cadherin expression seems to be associated with the tumor progression and metastasis caused by EMT and MET [78]. These findings demonstrate that reduced E-cadherin expression may be linked with epigenetic modulation, due to partial methylation of the E-cadherin promoter region. More recently, a role of TGF-β has been identified to maintain DNA methylation patterns during EMT and sustain the silencing of E-cadherin and other tight junction genes [79].


microRNAs (miRNAs) are a new class of EMT regulators, functioning to modify EMT-inducing transcription factors [80]. miRNAs act by inhibiting gene expression at the post-transcriptional level by suppression of sequence complementary mRNA targets [81]. Since each small interfering RNA can interact with dozens of target mRNAs, their impact on gene expression can be significant. Members of the miR-200 family suppress tumor cell motility and invasiveness in vitro and inhibit induction of EMT through downregulation of ZEB1 and ZEB2 and the subsequent increase in E-cadherin expression [82]. Down-regulation of miR-200 was sufficient to reduce E-cadherin expression and induce EMT. Thus, modulating the expression of particular miRNAs is likely to effect tumorigenesis and may play a role in tumor metastasis as well [83].

The presence of miR-335 has been linked with suppression of metastasis in human breast cancer [84]. In a study of 20 primary human breast tumors, the expression of miR-335 was inversely associated with the formation of metastatic lesions. Results suggested that miR-335 helped down-regulate metastatic genes in breast cancer, and the loss of miR-335 may serve as a negative prognostic indicator. The important role that miRNAs play in the regulation of EMT suggests they may also serve as potential therapeutic targets.

Transcription factors

At least ten transcription factors have been identified as EMT regulators during embryogenesis and/or carcinogenesis (Figure 3) [56, 85]. Besides ZEB1 and ZEB2, the following transcription factors directly repress the CDH1 gene encoding E-cadherin: SNAI1, SNAI2 (SLUG), SNAI3, TWIST1, FOXC1, FOXC2, GSC (goosecoid), and KLF8. Extensive cross-talk among these transcription factors is necessary to maintain mesenchymal cell phenotypes [85]. Furthermore, these EMT-inducing transcription factors play a role in acquisition of stem cell characteristics as these factors are expressed at much higher levels in CD44+/CD24- breast CSC-like cells than in more differentiated epithelial cells [44, 86].


The polycomb group protein Bmi-1 (B lymphoma Mo-MLV insertion region 1 homologue) also plays a role in epigenetic gene-silencing and affects embryonic development and oncogenesis [87]. Bmi-1's role in breast cancer invasion and metastasis has been speculated, but details remain elusive. Recently, Song and colleagues [88] studied the Bmi-1 role in human nasopharyngeal epithelial cells and showed a direct association between Bmi1-1 and EMT. This report also revealed that EMT induction was due to the direct binding of Bmi-1 to the phosphatase and tensin homologue (PTEN) locus causing down-regulated expression of PTEN. Subsequent activation of the PI3K/Akt pathway stabilized Snail and down-regulated E-cadherin. Bmi-1 may become a therapeutic target in CSCs, not only modulating the self-renewal potential of these cells, but also inhibiting EMT and preventing stem cells from acquiring invasive properties.

Immune regulation

In addition to its well known function for accelerating cell invasion, SNAI1 has been demonstrated to contribute to enhancing metastasis by inducing immunosuppression through multiple mechanisms [89]. SNAI1+ melanoma was observed to induce severe immunosuppression both in vitro and in vivo. In mice, enhanced tumor metastasis was achieved through suppression of nearly all antitumor effector cells at the local tumor site in vivo. Almost no infiltration of effector cells was observed. Thus, expression of SNAI1-induced EMT and immunosuppression of the tumor-bearing host accelerate tumor metastasis simultaneously. Even tumors expressing low levels of SNAI1 might cause immunosuppression, suggesting that targeting SNAI1 may help prevent metastasis.

Epidermal growth factor receptor regulation

Expression of the ErbB receptor tyrosine kinase family and their ligands has been detected in various cancers and is particularly important in breast cancer, where it is associated with poor prognosis. Epidermal growth factor receptor (EGFR) signaling can induce EMT, invasion and metastasis via induction of Snail and ZEB [90]. EGFR/ErbB2 heterodimers could induce mammary epithelial cells to acquire invasive properties [91]. Inhibition of ErbB receptor signaling reversed an aggressive inflammatory breast cancer phenotype into an epithelial-like phenotype in vitro [92]. Korkaya and colleagues [32] demonstrated that HER2 (ErbB2) stimulated the self-renewal of breast cancer stem cells through the PI3K/Akt pathway. Their study provided the therapeutic rationale to treat chemotherapy-resistant breast CSCs with the EGFR/HER2 inhibitor lapatinib [18]. Due to limited clinical efficacy with ErbB inhibitors alone, a combination approach may provide improved clinical outcome.

Microenvironment and cytokines

Extracellular factors related to tumor microenvironment are known to induce EMT. For example, matrix metalloproteinase (MMP) family proteins were found to cause mammary epithelial cell invasion by inducing EMT [93, 94]. It is likely that EMT-inducing signals are released by mesenchymal cells into the tumor microenvironment and confer on tumor cells invasive and metastatic characteristics [67]. Moreover, these signals are released by more activated and inflamed stroma surrounded by progressive tumors [76]. The concept of activated stroma releasing EMT-inducing transcription factors into the microenvironment suggests that when tumor cells metastasize to distant sites, these stromal cells may not be activated. In the absence of EMT-inducing signals, the metastasized tumor cells may revert to their epithelial phenotype (MET). For example, Mori and colleagues [95] reported that mouse mammary epithelial cells underwent malignant transformation, including loss of cell-to-cell contact, when exposed to long-term oxidative stress.


Preventing the induction of EMT in primary breast cancer may serve as a novel mechanism to potentially inhibit the spread of metastatic disease. Activation of embryonic signaling pathways (that is, Notch, Wnt, Hh, and TGF-β) and their downstream transcription factors are responsible for driving EMT, resulting in the transformation of epithelial-like CSCs into cells with aggressive mesenchymal-like phenotypes. These invasive CSCs have the potential to travel to distant sites and initiate metastatic tumors. Therapies directed towards inhibiting the induction of EMT may therefore reduce the formation of mesenchymal-like CSCs and improve clinical outcome.

In parallel with the development of this therapeutic approach, it is crucial to identify a more detailed molecular signature of the primary tumor in order to more accurately predict which patient population would benefit from this type of long-term therapy. By examining embryonic pathway signaling at the tumor microenviron-mental level, one may better predict future metastatic potential. In high risk patients, therapeutic approaches directed at modulation of embryonic signaling pathways may be beneficial.

Another promising approach to inhibit metastasis centers on developing molecular assays to identify which dormant tumor cells will acquire metastatic potential. Tumor dormancy is relatively unique to breast cancer as some disseminated tumor cells remain quiescent, while others metastasize to distant organs. For example, bone marrow micrometastases are commonly seen in early stage breast cancer. Targeting bone marrow disseminated tumors at an early stage with adjuvant therapy may be beneficial to those with high risk for future distant metastasis. Alternatively, therapies designed to mobilize or stimulate dormant CSCs to undergo cell division may subsequently increase their sensitivity to currently available cytotoxic therapeutics.

Finally, the interrelationship between CSCs, embryonic signaling pathways, and EMT/MET offers a continuum of potential therapeutic targets. Knowledge of this relationship is important for both the researcher and clinician in developing and administering optimal therapies for breast cancer patients.