Abstract
Toxoplasma gondii IgG antibodies were measured in 212 goat sera, comparing the Sabin-Feldman dye-test and a three-layer sandwich enzyme-linked immunosorbent assay (ELISA). With 98 % concordance obtained between these 2 tests, the results are at the same paragon as for human sera. Accordingly, the ELISA sandwich procedure appears to be suitable for large-scale analysis of goat sera. The discordant 2 % were ELISA positive and dye-test negative. One possible explanation of the divergent titres is given using an immunized goat model.
Sammendrag
IgG antistoffer mot Toxoplasma gondii ble malt i 212 geit ved hjelp av Sabin-Feldman’s dye test, og en tre-lags sandwich ELISA (“enzyme-linked immunosorbent assay”). Med 98 % sammenfallende resultater i disse 2 testene, var overensstemmelsen på lik lin je med hva man finner for humane sera. Derav følger at ELISA prosedyren
også bør egne seg for masseundersøkelser av geitesera.
De 2 % avvik var ELISA positive og dye-test negative. En mulig forklaring på denne divergensen er be&krevet ved hjelp av en immuni-sert geit-modell.
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Berdal, B.P., Olsvik, Ø., Alrnlid, T. et al. Comparison Between Elisa and Dye Test for Detection of Naturally Acquired Toxoplasma Gondii Antibodies in the Goat. Acta Vet Scand 24, 65–73 (1983). https://doi.org/10.1186/BF03546758
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DOI: https://doi.org/10.1186/BF03546758