Introduction
Increases in mucosal intestinal permeability may cause pathological leakage of bacterial products, inducing inflammation. Low-grade systemic inflammation has been reported in overweight humans. Probes used for permeability evaluation in humans have not yet been evaluated in dogs.
Objective
To determine suitability of human permeability probes (riboflavin, lactulose, mannitol and sucralose) for canine obesity models.
Methods
Fourteen healthy dogs were examined as baseline group. They were given all four probes per os using human doses corrected for weight and reported to be harmless for dogs. Urine samples were collected 2, 4 and 6 h after ingesting probes. Urinary excretion was quantified.
Results
The least squares means of mannitol proportion (excreted/ingested ± SE) was 15.6 % (± 2.0), 11.0 % (± 2.1) and 5.5 % (± 2.1) for urine sampled 2, 4 and 6 h after ingestion (p<0.0001). Hence, the majority of mannitol absorption and secretion representing upper gastrointestinal leakage was completed by 4 h. Riboflavin followed a similar temporal profile, defining 4 h as a cutoff for upper vs lower gut permeability. Probes were well tolerated.
Conclusions
Data obtained in the study indicates that probes used for humans can be used for intestinal permeability evaluation in dogs.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is published under an open access license. Please check the 'Copyright Information' section either on this page or in the PDF for details of this license and what re-use is permitted. If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and re-use information, please contact the Rights and Permissions team.
About this article
Cite this article
Hagman, R., Rosberg, P., Al-Saffar, A. et al. A new test for canine intestinal mucosa permeability. Acta Vet Scand 57 (Suppl 1), O16 (2015). https://doi.org/10.1186/1751-0147-57-S1-O16
Published:
DOI: https://doi.org/10.1186/1751-0147-57-S1-O16