Estrogen level monitoring in artificial frozen-thawed embryo transfer cycles using step-up regime without pituitary suppression: is it necessary?

  • Zhihong Niu
  • Yun Feng
  • Yijuan Sun
  • Aijun Zhang
  • Huiqin Zhang
Open Access



To discuss the meaning of serum oestradiol monitoring in frozen embryo transfer cycle using hormone replacement without pretreatment with gonadotropin hormone (GnRH) agonist.


The data from two hundred twelve women undergoing two hundred seventy-four frozen-thawed embryo transfer (FET) cycles was included in this retrospective cohort study. They were detected of serum oestradiol levels and endometrium thicknesses during hormone supplement FET cycles and compared their pregnancy outcomes according to their oestradiol level on progesterone initiation day.


Patients with different levels of serum oestradiol (percentile 0–25th, 25th–75th and 75th–100th) on progesterone initiation day yielded the endometrium thickness of 9.3 ± 0.12, 8.9 ± 0.07 and 9.1 ± 0.11 mm(P > 0.05) and the pregnancy rate of 32.2%, 38.4% and 36.3% (P > 0.05) respectively.


The serum estradiol level did not predict pregnancy success in hormone replacement FET cycles, suggesting that oestradiol monitoring in this method of endometrial preparation is unnecessary.



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© Niu et al; licensee BioMed Central Ltd. 2008

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Zhihong Niu
    • 1
    • 2
  • Yun Feng
    • 1
  • Yijuan Sun
    • 1
  • Aijun Zhang
    • 1
  • Huiqin Zhang
    • 2
  1. 1.IVF-unit, Department of Obstetrics and GynecologyRuiJin Hospital Affiliated to Shanghai Jiaotong UniversityPR China
  2. 2.Shanghai Institute of Planned Parenthood Research, Fudan UniversityShanghaiPR China

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