Dendrimers are nano-sized, radially symmetric molecules with well-defined, homogeneous, and monodisperse structure that has a typically symmetric core, an inner shell, and an outer shell. Their three traditional macromolecular architectural classes are broadly recognized to generate rather polydisperse products of different molecular weights. A variety of dendrimers exist, and each has biological properties such as polyvalency, self-assembling, electrostatic interactions, chemical stability, low cytotoxicity, and solubility. These varied characteristics make dendrimers a good choice in the medical field, and this review covers their diverse applications.
Dendrimers are nano-sized, radially symmetric molecules with well-defined, homogeneous, and monodisperse structure consisting of tree-like arms or branches . These hyperbranched molecules were first discovered by Fritz Vogtle in 1978, by Donald Tomalia and co-workers in the early 1980s, and at the same time, but independently by George R. Newkome. The second group called synthesized macromolecules ‘arborols’ means, in Latin, ‘trees’. Dendrimers might also be called ‘cascade molecules’ , but this term is not as much established as ‘dendrimers’ [2–4]. Dendrimers are nearly monodisperse macromolecules that contain symmetric branching units built around a small molecule or a linear polymer core [5–7]. ‘Dendrimer’ is only an architectural motif and not a compound. Polyionic dendrimers do not have a persistent shape and may undergo changes in size, shape, and flexibility as a function of increasing generations [8–10]. Dendrimers are hyperbranched macromolecules with a carefully tailored architecture, the end-groups (i.e., the groups reaching the outer periphery), which can be functionalized, thus modifying their physicochemical or biological properties [11–16]. Dendrimers have gained a broad range of applications in supramolecular chemistry, particularly in host-guest reactions and self-assembly processes. Dendrimers are characterized by special features that make them promising candidates for a lot of applications. Dendrimers are highly defined artificial macromolecules, which are characterized by a combination of a high number of functional groups and a compact molecular structure . The emerging role of dendritic macromolecules for anticancer therapies and diagnostic imaging is remarkable. The advantages of these well-defined materials make them the newest class of macromolecular nano-scale delivery devices . Dendritic macromolecules tend to linearly increase in diameter and adopt a more globular shape with increasing dendrimer generation. Therefore, dendrimers have become an ideal delivery vehicle candidate for explicit study of the effects of polymer size, charge, and composition on biologically relevant properties such as lipid bilayer interactions, cytotoxicity, internalization, blood plasma retention time, biodistribution, and filtration  (Figure 1).
Structure and chemistry
The structure of dendrimer molecules begins with a central atom or group of atoms labeled as the core. From this central structure, the branches of other atoms called ‘dendrons’ grow through a variety of chemical reactions. There continues to be a debate about the exact structure of dendrimers, in particular whether they are fully extended with maximum density at the surface or whether the end-groups fold back into a densely packed interior [21, 22]. Dendrimers can be prepared with a level of control not attainable with most linear polymers, leading to nearly monodisperse, globular macromolecules with a large number of peripheral groups as seen in Figure 2, the structure of some dendrimer repeat units, for example, the 1,3-diphenylacetylene unit developed by Moore .
Dendrimers are a new class of polymeric belongings. Their chemistry is one of the most attractive and hastily growing areas of new chemistry [25–27]. Dendrimer chemistry, as other specialized research fields, has its own terms and abbreviations. Furthermore, a more brief structural nomenclature is applied to describe the different chemical events taking place at the dendrimer surface. Dendrigrafts are a class of dendritic polymers like dendrimers that can be constructed with a well-defined molecular structure, i.e., being monodisperse . The unique structure of dendrimers provides special opportunities for host-guest chemistry (Figure 3) and is especially well equipped to engage in multivalent interactions. At the same time, one of the first proposed applications of dendrimers was as container compounds, wherein small substrates are bound within the internal voids of the dendrimer . Experimental evidence for unimolecular micelle properties was established many years ago both in hyperbranched polymers  and dendrimers .
Dendrimers are just in between molecular chemistry and polymer chemistry. They relate to the molecular chemistry world by virtue of their step-by-step controlled synthesis, and they relate to the polymer world because of their repetitive structure made of monomers [32–35]. The three traditional macromolecular architectural classes (i.e., linear, cross-linked, and branched) are broadly recognized to generate rather polydisperse products of different molecular weights. In contrast, the synthesis of dendrimers offers the chance to generate monodisperse, structure-controlled macromolecular architectures similar to those observed in biological systems [36, 37]. Dendrimers are generally prepared using either a divergent method or a convergent one . In the different methods, dendrimer grows outward from a multifunctional core molecule. The core molecule reacts with monomer molecules containing one reactive and two dormant groups, giving the first-generation dendrimer. Then, the new periphery of the molecule is activated for reactions with more monomers.
Cascade reactions are the foundation of dendrimer synthesis
The basic cascade or iterative methods that are currently employed for synthesis were known to chemists much earlier. For example, similar schemes form the basis of solid-phase peptide synthesis. In turn, biology has long exploited similar iterative strategies in biochemical synthetic pathways; one example is provided by fatty acid biosynthesis  (Figure 4).
The synthesis of dendrimers follows either a divergent or convergent approach
Dendrimers can be synthesized by two major approaches. In the divergent approach, used in early periods, the synthesis starts from the core of the dendrimer to which the arms are attached by adding building blocks in an exhaustive and step-wise manner. In the convergent approach, synthesis starts from the exterior, beginning with the molecular structure that ultimately becomes the outermost arm of the final dendrimer. In this strategy, the final generation number is pre-determined, necessitating the synthesis of branches of a variety of requisite sizes beforehand for each generation  (Figure 5).
Properties of dendrimers
When comparing dendrimers with other nanoscale synthetic structures (e.g., traditional polymers, buck balls, or carbon nanotubes), these are either highly non-defined or have limited structural diversity.
Pharmacokinetic properties are one of the most significant aspects that need to be considered for the successful biomedical application of dendrimers, for instance, drug delivery, imaging, photodynamic therapy, and neutron capture therapy. The diversity of potential applications of dendrimers in medicine results in increasing interest in this area. For example, there are several modifications of dendrimers' peripheral groups which enable to obtain antibody-dendrimer, peptide-dendrimer conjugates or dendritic boxes that encapsulate guest molecules .
Covalent conjugation strategies
The strategy of coupling small molecules to polymeric scaffolds by covalent linkages to improve their pharmacological properties has been under experimental test for over three decades [43–46]. In most cases, however, the conjugated dendritic assembly functions as ‘pro-drug’ where, upon internalization into the target cell, the conjugate must be liberated to activate the drug (Figure 6).
Polyvalency is useful as it provides for versatile functionalization; it is also extremely important to produce multiple interactions with biological receptor sites, for example, in the design of antiviral therapeutic agents.
Another fascinating and rapidly developing area of chemistry is that of self-assembly. Self-assembly is the spontaneous, precise association of chemical species by specific, complementary intermolecular forces. Recently, the self-assembly of dendritic structures has been of increasing interest . Because dendrimers contain three distinct structural parts (the core, end-groups, and branched units connecting the core and periphery), there are three strategies for self-assembling dendrimers. The first is to create dendrons with a core unit that is capable of recognizing itself or a ditopic or polytopic core structure, therefore leading to spontaneous formation of a dendrimer [48–51]. A self-assembling dendrimer using pseudorotaxane formation as the organizing force was reported by Gibson and coworkers (Figure 7) .
Molecular recognition events at dendrimer surfaces are distinguished by the large number of often identical end-groups presented by the dendritic host. When these groups are charged, the surface may have as a polyelectrolyte and is likely to electrostatically attract oppositely charged molecules . One example of electrostatic interactions between polyelectrolyte dendrimers and charged species include the aggregation of methylene blue on the dendrimer surface and the binding of EPR probes such as copper complexes and nitroxide cation radicals [54, 55].
Today, dendrimers have several medicinal and practical applications.
Dendrimers in biomedical field
Dendritic polymers have advantage in biomedical applications. These dendritic polymers are analogous to protein, enzymes, and viruses, and are easily functionalized. Dendrimers and other molecules can either be attached to the periphery or can be encapsulated in their interior voids . Modern medicine uses a variety of this material as potential blood substitutes, e.g., polyamidoamine dendrimers .
Perhaps the most promising potential of dendrimers is in their possibility to perform controlled and specified drug delivery, which regards the topic of nanomedicine. One of the most fundamental problems that are set toward modern medicine is to improve pharmacokinetic properties of drugs for cancer . Drugs conjugated with polymers are characterized by lengthened half-life, higher stability, water solubility, decreased immunogenicity, and antigenicity . Unique pathophysiological traits of tumors such as extensive angiogenesis resulting in hypervascularization, the increased permeability of tumor vasculature, and limited lymphatic drainage enable passive targeting, and as a result, selective accumulation of macromolecules in tumor tissue. This phenomenon is known as ‘enhanced permeation and retention’ (EPR) [58, 60]. The drug-dendrimer conjugates show high solubility, reduced systemic toxicity, and selective accumulation in solid tumors. Different strategies have been proposed to enclose within the dendrimer structure drug molecules, genetic materials, targeting agents, and dyes either by encapsulation, complexation, or conjugation.
Dendrimers in drug delivery
In 1982, Maciejewski proposed, for the first time, the utilization of these highly branched molecules as molecular containers . Host-guest properties of dendritic polymers are currently under scientific investigation and have gained crucial position in the field of supramolecular chemistry. Host-guest chemistry is based on the reaction of binding of a substrate molecule (guest) to a receptor molecule (host) .
Transdermal drug delivery
Clinical use of NSAIDs is limited due to adverse reactions such as GI side effects and renal side effects when given orally. Transdermal drug delivery overcomes these bad effects and also maintains therapeutic blood level for longer period of time. Transdermal delivery suffers poor rates of transcutaneous delivery due to barrier function of the skin. Dendrimers have found applications in transdermal drug delivery systems. Generally, in bioactive drugs having hydrophobic moieties in their structure and low water solubility, dendrimers are a good choice in the field of efficient delivery system .
The primary promise that the combination of understanding molecular pathways of disease and the complete human genome sequence would yield safer and more efficient medicines and revolutionize the way we treat patients has not been fulfilled to date. However, there is little doubt that genetic therapies will make a significant contribution to our therapeutic armamentarium once some of the key challenges, such as specific and efficient delivery, have been solved . The ability to deliver pieces of DNA to the required parts of a cell includes many challenges. Current research is being performed to find ways to use dendrimers to traffic genes into cells without damaging or deactivating the DNA. To maintain the activity of DNA during dehydration, the dendrimer/DNA complexes were encapsulated in a water soluble polymer and then deposited on or sandwiched in functional polymer films with a fast degradation rate to mediate gene transfection. Based on this method, PAMAM dendrimer/DNA complexes were used to encapsulate functional biodegradable polymer films for substrate-mediated gene delivery. Research has shown that the fast-degrading functional polymer has great potential for localized transfection [65–67].
Dendrimers as magnetic resonance imaging contrast agents
Dendrimer-based metal chelates act as magnetic resonance imaging contrast agents. Dendrimers are extremely appropriate and used as image contrast media because of their properties .
Dendrimers, although are single molecules, can contain high numbers of functional groups on their surfaces. This makes them striking for applications where the covalent connection or close proximity of a high number of species is important. Balzani and coworkers investigated the fluorescence of a fourth-generation poly (propylene amine) dendrimer decorated with 32 dansyl units at the periphery (Figure 8) . Since the dendrimer contains 30 aliphatic amine units in the interior, suitable metal ions are able to coordinate. It was observed that when a Co2+ ion is incorporated into the dendrimer, the strong fluorescence of all the dansyl units is quenched. Low concentrations of Co2+ ions (4.6 × 10-7 M) can be detected using a dendrimer concentration of 4.6 × 10-6 M. The many fluorescent groups on the surface serve to amplify the sensitivity of the dendrimer as a sensor .
Dendrimers used for enhancing solubility
PAMAM dendrimers are expected to have potential applications in enhancing solubility for drug delivery systems. Dendrimers have hydrophilic exteriors and interiors, which are responsible for its unimolecular micelle nature. Dendrimer-based carriers offer the opportunity to enhance the oral bioavailability of problematic drugs. Thus, dendrimer nano carriers offer the potential to enhance the bioavailability of drugs that are poorly soluble and/or substrates for efflux transporters [70, 71].
Photodynamic therapy (PDT) relies on the activation of a photosensitizing agent with visible or near-infrared (NIR) light. Upon excitation, a highly energetic state is formed which, upon reaction with oxygen, affords a highly reactive singlet oxygen capable of inducing necrosis and apoptosis in tumor cells. Dendritic delivery of PDT agents has been investigated within the last few years in order to improve upon tumor selectivity, retention, and pharmacokinetics [72–75].
Miscellaneous dendrimer applications
Clearly, there are many other areas of biological chemistry where application of dendrimer systems may be helpful. Cellular delivery using carrier dendritic polymers is used in the purification of water dendrimer-based product in cosmetics contaminated by toxic metal ion and inorganic solute, and dendrimer-based commercial products organic solutes . Furthermore, highly sensitive analytical devices [77, 78], MRI contrast agents , prion research , burn treatment , and EPR imaging with spin-labeled dendrimers [82–106] are some of the diverse areas of fascinating ongoing dendrimer research that are beyond the scope of this article.
Dendrimers are characterized by individual features that make them hopeful candidates for a lot of applications. Dendrimers are highly defined artificial macromolecules, which are characterized by a combination of a high number of functional groups and a compact molecular structure. A rapid increase of importance in the chemistry of dendrimers has been observed since the first dendrimers were prepared. Work was established to determine the methods of preparing and investigating the properties of the novel class of macro and micromolecules. In spite of the two decades since the finding of dendrimers, the multi-step synthesis still requires great effort.
Srinivasa-Gopalan S, Yarema KJ: Nanotechnologies for the Life Sciences: Dendrimers in Cancer Treatment and Diagnosis, Volume 7. New York: Wiley; 2007.
Klajnert B, Bryszewska M: Dendrimers: properties and applications. Acta Biochim Pol 2001, 48: 199–208.
Tomalia DA, Frechet JMJ: Discovery of dendrimers and dendritic polymers: a brief historical perspective. J Polym Sci A Polym Chem 2002, 40: 2719–2728.
Tomalia DA: The dendritic state. Mater Today 2005, 8: 34–36.
Tomalia DA, Baker H, Dewald J, Hall M, Kallos M, Martin S, Roeck J, Ryder J, Smith P: A new class of polymers: starburst-dendritic macromolecules. Polym J (Tokyo) 1985, 17: 117.
Newkome GR, Yao Z-Q, Baker GR, Gupta VK: Cascade molecules: a new approach to micelles. J Org Chem 1985, 50: 2003.
Hawker CJ, Frechet JMJ: Preparation of polymers with controlled molecular architecture: a new convergent approach to dendritic macromolecules. J Am Chem Soc 1990, 112: 7638–7647.
De Gennes PG, Hervet H: Statistics of starburst polymers. J de Physique Lett (Paris) 1983, 44: 9–351.
Mansfield ML, Klushin LI: Monte Carlo studies of dendrimer macromolecules. Macromolecules 1993, 26: 4262.
Bhalgat MK, Roberts JC: Molecular modeling of polyamidoamine (PAMAM) Starburst™ dendrimers. Eur Polym J 2000, 36: 647–651.
Bosman AW, Meijer EW: About dendrimers: structure, physical properties, and applications. Chem Rev 1999, 99: 1665–1688.
Gilles ER, Frechet JMJ: Dendrimers and dendritic polymers in drug delivery. Drug Discov Today 2005, 10: 35–43.
Tomalia DA, Baker H, Dewald JR, Hall M, Kallos G, Martin S, Roeck J, Ryder J, Smith P: Dendrimers II: architecture, nanostructure and supramolecular chemistry. Macromolecules 1986, 19: 2466.
Kim Y, Zimmerman SC: Applications of dendrimers in bio-organic chemistry. Curr Opin Chem Biol 1998, 2: 733–742.
Smith DK, Diederich F: Functional dendrimers: unique biological mimics. Chem Eur J 1998, 4: 1353–1361.
Stiriba S-E, Frey H, Haag R: Dendritic polymers in biomedical applications: from potential to clinical use in diagnostics and therapy. Angew Chem Int Ed 2002, 41: 1329–1334.
Tomalia DA, Frechet JMJ: Discovery of dendrimers and dendritic polymers: a brief historical perspective. J Polym Sci Part A 2002, 40: 2719.
Wolinsky JB, Grinstaff MW: Therapeutic and diagnostic applications of dendrimers for cancer treatment. Adv Drug Deliv Rev 2008, 60: 1037–1055.
Svenson S, Tomalia DA: Dendrimers in biomedical applications—reflections on the field. Adv Drug Deliv Rev 2005, 57: 2106–2129.
Tomalia DA, Baker H, Dewald J, Hall M, Kallos G, Martin S, Roeck J, Ryder J, Smith P: Dendritic macromolecules: synthesis of starburst dendrimers. Macromolecules 1986, 19: 2466–2468.
Zimmerman SC: Dendrimers in molecular recognition and self-assembly. Curr Opin Colloid Interfac Sci 1997, 2: 89.
Zeng FW, Zimmerman SC: Dendrimers in supramolecular chemistry: from molecular recognition to self-assembly. Chem Rev 1997, 97: 1681.
Moore JS: Shape-persistent molecular architectures of nanoscale dimension. Acc Chem Res 1997, 30: 402.
Zimmerman SC, Lawless LJ: Topics in Current Chemistry: Supramolecular Chemistry of Dendrimers, Volume 217. New York: Springer; 2001.
Boris D, Rubinstein M: A self-consistent mean field model of a starburst dendrimers: dense core vs. dense shells. Macromolecules 1996, 29: 7251–7260.
Tomalia DA, Baker H, Dewald JR, Hall M, Kallos G, Martin S: A new class of polymers: starburst-dendritic macromolecules. Polym J 1985, 17(1):117–132.
Spataro G, Malecaze F, Turrin CO, Soler V, Duhayon C, Elena PP: Designing dendrimers for ocular drug delivery. Eur J Med Chem 2010, 45(1):326–334.
Tomalia DA, Hedstrand DM, Ferritto MS: Comb-burst dendrimer topology: new macromolecular architecture derived from dendritic grafting. Macromolecules 1991, 24: 1435.
Maciejewski M: Concepts of trapping topologically by shell molecules. J Macromol Sci Chem 1982, A17: 689.
Kim YH, Webster OW: Water soluble hyperbranched polyphenylene: “a unimolecular micelle?”. J Am Chem Soc 1990, 112: 4592.
Newkome GRM, Baker GR, Saunders MJ, Grossman SH: Uni-molecular micelles. Angew Chem Int Ed Engl 1991, 30: 1178.
Frechet JMJ, Tomalia DA: Dendrimers and Other Dendritic Polymers. Chichester: Wiley; 2001.
Newkome GR, Moorefield CN, Vögtle F: Dendrimers and Dendrons: Concepts, Syntheses, Applications. Wiley: Weinheim; 2001.
Majoral JP, Caminade AM: Dendrimers containing heteroatoms (Si, P, B, Ge, or Bi). Chem Rev 1999, 99: 845–880.
Bosman AW, Janssen HM, Meijer EW: About dendrimers: structure, physical properties, and applications. Chem Rev 1999, 99: 1665–1688.
Tomalia DA: Birth of a new macromolecular architecture: dendrimers as quantized building blocks for nanoscale synthetic organic chemistry. Aldrichimica Acta 2004, 37: 39–57.
Tomalia DA: Dendrimer molecules. Sci Am 1995, 272: 62–66.
Hodge P: Polymer science branches out. Nature 1993, 362: 18–19.
Gitsov I, Lin C: Dendrimers – nanoparticles with precisely engineered surfaces. Curr Org Chem 2005, 9: 1025–1051.
Buhleier E, Wehner W, Vögtle F: “Cascade”- and “nonskid-chain-like” synthesis of molecular cavity topologies. Synthesis 1978, 1978(2):155–158.
Grayson SM, Frechet JMJ: Convergent dendrons and dendrimers: from synthesis to applications. Chem Rev 2001, 101: 3819–3868.
Szymanski P, Markowicz M, Mikiciuk-Olasik E: Nanotechnology in pharmaceutical and biomedical applications: Dendrimers. Nano Brief Rep Rev 2011, 6: 509–539.
Ringsdorf H: Structure and properties of pharmacologically active polymers. J Polym Sci Polym Symp 1975, 51: 135–153.
Bader H, Ringsdorf H, Schmidt B: Water-soluble polymers in medicine. Angew Makromol Chem 1984, 123/124: 457–485.
Gillies ER, Dy E, Frechet JMJ, Szoka FC: Biological evaluation of polyester dendrimer: poly (ethylene oxide) “bow-tie” hybrids with tunable molecular weight and architecture. Mol Pharm 2005, 2: 129–138.
Kolhe P, Khandare J, Pillai O, Kannan S, Lieh-Lai M, Kannan RM: Preparation, cellular transport, and activity of polyamidoamine-based dendritic nanodevices with a high drug payload. Biomaterials 2006, 27: 660–669.
Emrick T, Fréchet JMJ: Self-assembly of dendritic structures. Curr Opin Coll Interface Sci 1999, 4: 15–23. CrossRef, Web of Science® Times Cited: 80 CrossRef, Web of Science® Times Cited: 80
Christine D, Ijeoma FU, Andreas GS: Dendrimers in gene delivery. Adv Drug Deliv Rev 2005, 57: 2177–2202.
Wang Y, Zeng FW, Zimmerman SC: Dendrimers with anthyridine-based hydrogen-bonding units at their cores - synthesis, complexation and self-assembly studies. Tetrahedron Lett 1997, 38: 5459.
Kolotuchin SV, Zimmerman SC: Self-assembly mediated by the donor-donor-acceptor, acceptor-acceptor-donor (DDA, AAD) hydrogen-bonding motif: formation of a robust hexameric aggregate. J Am Chem Soc 1998, 120: 9092.
Issberner J, Vogtle F, Decola L, Balzani V: Dendritic bipyridine ligands and their tris(bipyridine)ruthenium(II) chelates—syntheses, absorption spectra, and photophysical properties. Chem Eur J 1997, 3: 706.
Gibson HW, Hamilton L, Yamaguchi N: Molecular self-assembly of dendrimers, non-covalent polymers and polypseudorotaxanes. Polym Adv Technol 2000, 11: 791.
Zeng F, Zimmerman SC: Dendrimers in supramolecular chemistry: from molecular recognition to self-assembly. Chem Rev 1997, 97: 1681–1712.
Ottaviani MF, Bossmann S, Turro NJ, Tomalia DA: Characterization of starburst dendrimers by the EPR technique. 1. Copper complexes in water solution. J Am Chem Soc 1994, 116: 661–671.
Ottaviani MF, Cossu E, Turro NJ, Tomalia DA: Characterization of starburst dendrimers by electron paramagnetic resonance. 2. Positively charged nitroxide radicals of variable chain length used as spin probes. J Am Chem Soc 1995, 117: 4387–4398.
Patel HN, Patel DRPM: Dendrimer applications – a review. Int J Pharm Bio Sci 2013, 4(2):454–463.
Ruth D, Lorella I: Dendrimer biocompatibility and toxicity. Ad Drug Deliv Rev 2005, 57: 2215–2237.
Sampathkumar SG, Yarema KJ: Chapter 1: dendrimers in cancer treatment and diagnosis. In Nanotechnologies for the Life Sciences. Volume 6: Nanomaterials for Cancer Diagnosis and Therapy. Edited by: Kumar CSSR. Hoboken: Wiley; 2007:1–47.
Pasut G, Veronese FM: Polymer - drug conjugation, recent achievements and general strategies. Prog Polym Sci 2007, 32: 933.
Gillies ER, Frechet JMJ: Dendrimers and dendritic polymers in drug delivery. DDT 2005, 10(1):35–43.
Maciejewski M: Concepts of trapping topologically by shell molecules. J Macromol Sci Chem A 1982, 17: 689.
Herrmann A, Mihov G, Vandermeulen GWM, Klok H-A, Mullen K: Peptide-functionalized polyphenylene dendrimers. Tetrahedron 2003, 59: 3925.
Cheng Y, Man N, Xu T, Fu R, Wang X, Wang X, Wen L: Transdermal delivery of nonsteroidal anti-inflammatory drugs mediated by polyamidoamine (PAMAM) dendrimers. J Pharm Sci 2007, 96: 595–602.
Pearson S, Jia H, Kandachi K: China approves first gene therapy. Nat Biotechnol 2004, 22: 3–4.
Fu H-L, Cheng S-X, Zhang X-Z, Zhuo R-X: Dendrimer/DNA complexes encapsulated functional biodegradable polymer for substrate-mediated gene delivery. J Gene Med 2008, 10(12):1334–1342.
Fu HL, Cheng SX, Zhang XZ: Dendrimer/DNA complexes encapsulated in a water soluble polymer and supported on fast degrading star poly (DL-lactide) for localized gene delivery. J Gene Med 2007, 124(3):181–188.
Tathagata D, Minakshi G, Jain NK: Poly (propyleneimine) dendrimer and dendrosome based genetic immunization against hepatitis B. Vaccine 2008, 26(27–28):3389–3394.
Balzani V, Ceroni P, Gestermann S, Kauffmann C, Gorka M, Vögtle F: Dendrimers as fluorescent sensors with signal amplification. Chem Commun 2000, 2000: 853–854.
Beer PD, Gale PA, Smith DK: Supramolecular Chemistry. Oxford: Oxford University Press; 1999.
Tomalia DA, Baker H, Dewald JR, Hall M, Kallos G, Martin S, Roeck J, Ryder J, Smith P: Dendrimers II: architecture, nanostructure and supramolecular chemistry. Macromolecules 1986, 19: 2466.
Froehling PE: Dendrimers and dyes – a review. Dyes Pigments 2001, 48: 187–195.
Triesscheijn M, Baas P, Schellens JH, Stewart FA: Photodynamic therapy in oncology. Oncologist 2006, 11: 1034–1044.
Nishiyama N, Stapert HR, Zhang GD, Takasu D, Jiang DL, Nagano T, Aida T, Kataoka K: Light-harvesting ionic dendrimer porphyrins as new photosensitizers for photodynamic therapy. Bioconjug Chem 2003, 14: 58–66.
Zhang GD, Harada A, Nishiyama N, Jiang DL, Koyama H, Aida T, Kataoka K: Polyion complex micelles entrapping cationic dendrimer porphyrin: effective photosensitizer for photodynamic therapy of cancer. J Control Release 2003, 93: 141–150.
Battah SH, Chee CE, Nakanishi H, Gerscher S, MacRobert AJ, Edwards C: Synthesis and biological studies of 5-aminolevulinic acid containing dendrimers for photodynamic therapy. Bioconjug Chem 2001, 12: 980–988.
Tiwari DK, Behari J, Sen P: Application of nanoparticles in waste water treatment. World Appl Sci J 2008, 3: 417–433.
Yoon HC, Lee D, Kim H-S: Reversible affinity interactions of antibody molecules at functionalized dendrimer monolayer: affinity-sensing surface with reusability. Anal Chim Acta 2002, 456: 209–218.
Benters R, Niemeyer CM, Drutschmann D, Blohm D, Wohrle D: DNA microarrays with PAMAM dendritic linker systems. Nucleic Acid Res 2002, 30: 1–11.
Konda SD, Wang S, Brechbiel M, Wiener EC: Biodistribution of a 153Gd-folate dendrimer, generation = 4, in mice with folate-receptor positive and negative ovarian tumor xenografts. Invest Radiol 2002, 37: 199–204.
Supattapone S, Nishina K, Rees JR: Pharmacological approaches to prion research. Biochem Pharmacol 2002, 63: 1383–1388.
Halkes SBA, Vrasidas I, Rooijer GR, van den Berg AJJ, Liskamp RMJ, Pieters RJ: Synthesis and biological activity of polygalloyl-dendrimers as stable tannic acid mimics. Bioorg Med Chem Lett 2002, 12: 1567–1570.
Yordanov AT, Yamada K-I, Krishna MC, Mitchell JB, Woller E, Cloninger M, Brechbiel MW: Spin-labeled dendrimers in EPR imaging with low molecular weight nitroxides. Angew Chem Int Ed Engl 2001, 40: 2690–2692.
Akbarzadeh A, Mikaeili H, Asgari D, Zarghami N, Mohammad R, Davaran S: Preparation and in-vitro evaluation of doxorubicin-loaded Fe3O4 magnetic nanoparticles modified with biocompatible copolymers. Int J Nanomed 2012, 7: 511–526.
Abolfazl A, Nosratollah Z, Haleh M, Davoud A, Amir Mohammad G, Khaksar Khiabani H, Soodabeh D: Synthesis, characterization and in vitro evaluation of novel polymer-coated magnetic nanoparticles for controlled delivery of doxorubicin. Inter J Nanotechnol Sci Environ 2012, 5: 13–25.
Akbarzadeh A, Samiei M, Joo SW, Anzaby M, Hanifehpour Y, Nasrabadi HT, Davaran : Synthesis, characterization and in vitro studies of doxorubicin-loaded magnetic nanoparticles grafted to smart copolymers on A549 lung cancer cell line. J Nanobiotechnol 2012, 10: 46–58.
Zohreh E, Nosratollah Z, Manoutchehr K, Soumaye A, Abolfazl A, Mohammad R, Zohreh Mohammad T, Kazem N-K: Inhibition of hTERT gene expression by silibinin-loaded PLGA-PEG-Fe3O4 in T47D breast cancer cell line. Bio Impacts 2013, 3(2):67–74.
Soodabeh D, Samira A, Kazem N-K, Hamid Tayefi N, Abolfazl A, Amir Ahmad K, Mojtaba A, Somayeh A: Synthesis and study of physicochemical characteristics of Fe3O4 magnetic nanocomposites based on poly(nisopropylacrylamide) for anti-cancer drugs delivery. Asian Pac J Cancer Prev 2014, 15(1):049–054.
Rogaie R-S, Nosratollah Z, Abolfazl B, Akram E, Abolfazl A, Mustafa R-T: Studies of the relationship between structure and antioxidant activity in interesting systems, including tyrosol, hydroxytyrosol derivatives indicated by quantum chemical calculations. Soft 2013, 2: 13–18.
Kazem N-K, Abolfazl A, Mohammad P-M, San Woo J: Inhibition of leptin and leptin receptor gene expression by silibinin-curcumin combination. Asian Pac J Cancer Prev 2013, 14(11):6595–6599.
Ghasemali S, Nejati-Koshki K, Akbarzadeh A, Tafsiri E, Zarghami N, Rahmati-Yamchi M, Alizadeh E, Barkhordari A, Tozihi M, Kordi S: Study of inhibitory effect of β-cyclodextrin-helenalin complex on HTERT gene expression in T47D breast cancer cell line by real time quantitative PCR (q-PCR). Asian Pac J Cancer Prev 2013, 14(11):6949–6953.
Mollazade M, Nejati-Koshki K, Abolfazl A, Younes H, Zarghami N, Sang Woo J: PAMAM dendrimers augment inhibitory effects of curcumin on cancer cell proliferation: possible inhibition of telomerase. Asian Pac J Cancer Prev 2013, 14(11):6925–6928.
Soodabeh D, Akbar R, Somayeh A, Amir Ahmad K, Kazem N-K, Hamid Tayefi N, Abolfazl A: Synthesis and physicochemical characterization of biodegradable star-shaped poly lactide-co-glycolide–β-cyclodextrin copolymer nanoparticles containing albumin. Adv Nanoparticles 2014, 3: 14–22.
Soodabeh D, Abolfazl A, Kazem N-K, Somayeh A, Mahmoud Farajpour G, Mahsa Mahmoudi S, Akbar R, Amir Ahmad K: In vitro studies of NIPAAM-MAA-VP copolymer-coated magnetic nanoparticles for controlled anticancer drug release. J Encapsul Adsorption Sci 2013, 3: 108–115.
Ahmadi A, Shirazi H, Pourbagher N, Akbarzadeh A, Omidfar K: An electrochemical immunosensor for digoxin using core-shell gold coated magnetic nanoparticles as labels. Mol Biol Rep 2014, 41(3):1659–1668.
Abolfazl A, Samiei M, Soodabeh D: Magnetic nanoparticles: preparation, physical properties and applications in biomedicine. Nanoscale Res Lett 2012, 7: 144–157.
Alireza V, Haleh M, Mohammad S, Samad Mussa F, Nosratollah Z, Mohammad K, Abolfazl A, Soodabeh D: Quantum dots: synthesis, bioapplications, and toxicity. Nanoscale Res Lett 2012, 7: 276.
Abolfazl A, Rogaie R-S, Soodabeh D, Sang Woo J, Nosratollah Z, Younes H, Mohammad S, Mohammad K, Kazem N-K: Liposome: classification, preparation, and applications. Nanoscale Res Lett 2013, 8: 102.
Mohammad P-M, Mohammad R-Y, Abolfazl A, Hadis D, Kazem N-K, Younes H, Sang Woo J: Protein detection through different platforms of immuno-loop-mediated isothermal amplification. Nanoscale Res Lett 2013, 8: 485.
Mohammad K, Ali V, Abolfazl A, Younes H, Sang Woo J: Investigation of quadratic electro-optic effects and electro absorption process in GaN/AlGaN spherical quantum dot. Nanoscale Res Lett 2014. in press in press
Fariba B, Alireza V, Kazem B, Samane M, Samad Mussa F, Nasrin S, Najme Malekzadeh G, Abolfazl A, Younes H, Sang Woo J, Mohammad R-Y: Nanodetection and nanodrug delivery in lung cancer. Nano Rev 2014. in press in press
Sohrabi N, Sohrabi Z, Valizadeh A, Mohammadi S, Mussa Farkhani S, Malekzadeh Gonabadi N, Mohammadi M, Badrzade F, Akbarzadeh A, Woo Joo S, Hanifehpour Y: Basic of DNA biosensors and cancer diagnosis. Nano Rev 2014. in press in press
Tabatabaei Mirakabad FS, Akbarzadeh A, Zarghami N, Zeighamian V, Rahimzadeh A, Alimohammadi S: PLGA-cased nanoparticles as cancer drug delivery systems. APJCP 2014, 15(1):517–535.
Valizadeh H, Mohammadi G, Ehyaei R, Milani M, Azhdarzadeh M, Zakeri-Milani P, Lotfipour F: Antibacterial activity of clarithromycin loaded PLGA nanoparticles. Pharmazie Int J Pharm Sci 2012, 67(1):63–68.
Hasani A, Sharifi Y, Ghotaslou R, Naghili B, Aghazadeh M, Milani M: Molecular screening of virulence genes in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolated from clinical specimens in Northwest Iran. Indian J Med Microbiol 2012, 30: 2.
Sharifi Y, Hasani A, Ghotaslou R, Varshochi M, Hasani A, Soroush MH, Aghazadeh M, Milani M: Vancomycin-resistant Enterococci among clinical isolates from north-west Iran: identification of therapeutic surrogates. J Med Microbiol 2012, 61(4):600–602.
Farajnia S, Hassan M, HallajNezhadi S, Mohammadnejad L, Milani M, Lotfipour F: Determination of indicator bacteria in pharmaceutical samples by multiplex PCR. J Rapid Meth Aut Mic 2009, 17(3):328–338.
The authors thank the Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences of Tabriz University of Medical Sciences for all the support provided. This work is funded by Grant 2011-0014246 of the National Research Foundation of Korea.
The authors declare that they have no competing interests.
SWJ conceived the study and participated in its design and coordination. EA participated in the sequence alignment and drafted the manuscript. AA, RPA, SFA, HTN, YH, KNK, and MM helped in drafting the manuscript. All authors read and approved the final manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Abbasi, E., Aval, S.F., Akbarzadeh, A. et al. Dendrimers: synthesis, applications, and properties. Nanoscale Res Lett 9, 247 (2014). https://doi.org/10.1186/1556-276X-9-247