Polyethylenimine (PEI), a cationic polymer, is one of the most efficient non-viral vectors for transgene therapy. Decorin (DCN), a leucine-rich proteoglycan secreted by glomerular mesangial cells (MC), is a promising anti-fibrotic agent for the treatment of glomerulonephritis. In this study, we used PEI–DCN nanocomplexes with different N/P ratios to transfect MC in vitro and deliver the MC vector with PEI–DCN expressing into rat anti-Thy1.1 nephritis kidney tissue via injection into the left renal artery in vivo. The PEI–plasmid DNA complex at N/P 20 had the highest level of transfection efficiency and the lowest level of cytotoxicity in cultured MC. Following injection, the ex vivo gene was transferred successfully into the glomeruli of the rat anti-Thy1.1 nephritis model by the MC vector with the PEI–DCN complex. The exogenous MC with DCN expression was located mainly in the mesangium and the glomerular capillary. Over-expression of DCN in diseased glomeruli could result in the inhibition of collagen IV deposition and MC proliferation. The pathological changes of rat nephritis were alleviated following injection of the vector. These findings demonstrate that the DCN gene delivered by the PEI–DNA nanocomplex with the MC vector is a promising therapeutic method for the treatment of glomerulonephritis.
Gene therapy is a promising strategy for treatment of inheritable or acquired diseases[1–3]. One of the key factors for successful gene therapy is the development of safe and efficient gene delivery vectors. Accumulating data show that one of the most common using non-viral vectors in vivo and in vitro is polyethylenimine (PEI)[4, 5]. PEI is a highly water-soluble cationic polymer in which every two carbon atoms are followed by a nitrogen atom. Under physiological conditions, approximately 20% of the nitrogens of PEI are protonated and, as a result, the ionization state of the polymer can be changed over a broad pH range. The positive charge of PEI results in effective binding to the negatively charged plasmid DNA (pDNA), forming a nanoparticle of the pDNA complex that is small enough to be transported easily through the cytoplasm and to protect the pDNA from nuclease degradation[7, 8]. PEI has two structural forms, linear and branched, with various molecular masses. Some studies have shown that 25 kDa branched PEI can be used as a gene vector that has many advantages, such as high transfection efficiency, low cytotoxicity, no immune reaction and easy modification in vitro[9–11]. PEI has been used successfully in many experiments and clinical trials[4, 12]. For example, the intraperitoneal injection route of administration of PEI–DNA is considered to be promising for the treatment of a variety of diseases, including carcinoma of the stomach, pancreas and ovary[13, 14]. Whether by aerosol inhalation or by the intranasal route, the PEI–DNA complex is potentially useful as a therapeutic approach for many lung diseases, such as cancer and asthma. Delivery of the p53 tumor suppressor gene and PEI complex by aerosol can inhibit lung metastasis induced by a melanoma. When administered by the intranasal route, the IL-4 antisense oligodeoxynucleotides–PEI complexes are capable of inhibiting IL-4 secretion in a sensitized murine model of airway inflammation, which is thought to be an effective therapy for asthma.
Glomerulosclerosis, the end stage of various progressive glomerular diseases, is characterized by over-expression of TGF-β1 and extracellular matrix deposition. Decorin (DCN) is a leucine-rich proteoglycan secreted by glomerular mesangial cells (MC) in kidney. DCN can bind to TGF-β and neutralize its biological activity, inhibit the matrix deposition in glomeruli induced by over-expression of TGF-β1 and, thus, inhibit the development of glomerulosclerosis. Earlier, we showed that the over-expression of DCN could inhibit MC growth, promote cell apoptosis, and down-regulate the protein levels of TGF-β1 and collagen type IV in vitro. Using the hemagglutinating virus of Japan; Sendao virus (HVJ)-liposome method to transfer a DCN plasmid vector into rat skeletal muscle, Isaka and colleagues found increased amounts of DCN protein in skeletal muscle and in kidney with a marked therapeutic effect on rat glomerulonephritis. Therefore, DCN is a promising anti-fibrotic agent for the treatment of glomerulonephritis.
Successful gene therapy is dependent on the choice of a sensitive gene and a suitable gene vector, and identification of the correct target. Therefore, it is essential in gene therapy research to explore the way of directional gene delivery to target organ. In a study of glomerular disease, it is found that the exogenous mesangial cell transferred into kidney by renal artery injection has a feature of “homing instinct” and can migrate to the mesangium of glomeruli. Kim et al. transferred rat MC with β-galactosidase expressing plasmid as a cell vector into a rat left kidney. Cells expressing enzyme activity were located in the glomerular capillary and mesangium in 80% of the glomeruli 1 day later. This study provides evidence that transfer of an ex vivo gene to glomeruli is feasible and might prove to be a useful tool for future investigation of glomerular diseases. We thus used the MC cell vector to deliver a PEI–DCN expression plasmid into an animal nephritis model for gene therapy research.
In this study, we used a PEI– DCN nanocomplex as a gene vector to transfect cultured mesangial cells and investigated the effect of DCN in vitro. Subsequently, we transferred the PEI– DCN expressing MC into the left kidney of a rat anti-Thy1.1 glomerulonephritis model via left renal artery injection and assessed the feasibility of gene therapy delivered by a PEI nanocomplex in glomerular diseases.
Polyethylenimine (PEI, branched, 25 kDa) and the monoclonal antibody to β-actin were purchased from Sigma-Aldrich (St. Louis, MO). The monoclonal anti-DCN antibody was from R&D Systems (Minneapolis, MN). The monoclonal anti-vimentin antibody was from Chamngdao Biotechnology (Shanghai, China). The monoclonal antibody to transforming growth factor (TGF) β1 and the rabbit polyclonal antibody to collagen IV were from Abcam (Cambridge, UK). The monoclonal antibody to green fluorescent protein (GFP) was from Cell Signaling Technology (Danvers, MA). The monoclonal antibody to proliferating cell nuclear antigen (PCNA) was from Santa Cruz Biotechnology (Santa Cruz, CA). Peroxidase-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were from Jackson ImmunoResearch (West Grove, PA).
Cell culture and plasmids
Mesangial cells were prepared from the kidney cortex of male Sprague–Dawley rats as described. Cells maintained in Dulbecco’s modified Eagle medium supplemented with 10% (v/v) fetal bovine serum at 37°C in a humidified 5% (v/v) CO2 atmosphere were used between passages 6 and 10. Plasmid pcDNA3.1A-DCN made in our laboratory and plasmid pEGFP (Clontech, Mountain View, CA) were amplified in Escherichia coli and purified by the Hispeed Plasmid Midi Kit (QIAGEN) according to the manufacturer’s protocol.
Preparation of the PEI–pDNA nanocomplex
Branched 25 kDa PEI and pDNA were each dissolved in phosphate-buffered saline (PBS, pH 7.4). Equal volumes of the PEI and pDNA solutions were mixed together to give PEI nitrogen to pDNA phosphate (N/P) ratios of 1, 5, 10, 15 and 20, shaken for 30 s and then kept at room temperature for 30 min. The diameter and zeta potential of the complexes were determined by a laser particle size analyzer (Nicomp™ 380 PSS, CA).
Transfection of PEI–pEGFP or PEI–DCN into MC
pEGFP was used as a reporter gene to monitor gene expression. The preparation of PEI– pEGFP complexes at various N/P ratios is described in the preceding section. At 24 h before transfection, mesangial cells were seeded into 6-well plates at a density of 3 × 105 cells/well. At the time of transfection, the cells were washed twice with PBS, 200 μl mixture solution containing 4 μg PEI– pEGFP complex was added to each well followed by incubation for 6 h at 37°C, the transfection agent then was replaced by fresh DMEM containing 10% (v/v) fetal bovine serum and the cells were incubated for 48 h at 37°C. Transfection of pEGFP with Lipofectamine™ 2000 (Invitrogen, Carlsbad) prepared according to the manufacturer’s protocol was used as a positive control. At 48 h following transfection, expression of GFP in transfected cells was observed by inverted fluorescence microscopy. Cells were harvested and used to quantify the transfection efficiency of PEI by flow cytometry (FACScan, BD Biosciences, MD, USA). All transfection experiments were done in triplicate.
MC were grown as described in the preceding section and treated with PEI– pDCN complex at an N/P ratio of 20. At 3 days after transfection, geneticin (G418, Sigma) at a concentration of 0.6 mg/ml was added to the cells. Three weeks later, positive clones were detected by Western blotting after culture expansion.
MC cells were seeded in 96-well plates at a density of 8 × 103 cells/well before transfection and incubation for 24 h at 37°C. The cells were transfected by PEI– pEGFP at different N/P ratios as described above and incubated for another 48 h at 37°C. Cells treated with naked plasmid were used as a negative control. The cells were transfected with Lipofectamine™ 2000 as a positive control. Cytotoxicity was assessed using the Cell Counting Kit8 (Dojindo, Tokyo, Japan) according to the manufacturer’s instructions. Relative cell viability was calculated as:
Cells were harvested in lysis buffer (20 mM Tris at pH 8.0, 150 mM NaCl, 1% (v/v) Nonidet P-40, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM Na3VO4, 1 mM NaF and 1 μg/ml each of aprotinin, leupeptin and pepstatin) on ice. Proteins (700 μg) of were separated by SDS-PAGE (10% (w/v) polyacrylamide gel) and transferred onto a polyvinylidene fluoride membrane. After blocking with 5% non-fat milk, the membrane was probed with the appropriate primary antibody overnight on a shaker at 4°C and proteins were detected by enzyme-linked chemiluminescence using an ECL kit (Thermo Scientific, Rockford, IL).
Analysis of the cell cycle was done by flow cytometry. MC were digested from culture plates by 0.05% (w/v) trypsin and treated with citrate buffer for 30 min, then incubated with RNase for a few minutes followed by addition of propidium iodide for 15 min. The results were analyzed by CellQuest software (BD Bioscience, Mississauga, Ontario, Canada) at the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences.
In vivo experimental design
A rat anti-Thy1.1 glomerulonephritis model was made by injection of rabbit anti-rat thymocyte serum (ATS) into the tail vein of male Sprague–Dawley rats as described. At day 3, the rats were anesthetized by an intraperitoneal injection of 10% (w/v) chloral hydrate (0.4 ml/100 g body weight). The left kidney was exposed through a left flank incision, the renal artery was separated and blood flow was interrupted by vessel clamping. MC with PEI–PEGFP or PEI–DCN were trypsinized, suspended in 800 μl of PBS with cell concentration 1 × 106, and injected into the left renal artery using a 29-gauge needle at a rate of 70 μl/s. The cells remained in the left kidney for 5 min and then the blood was allowed to re-perfuse. For the control group, normal mesangial cells were injected into the left renal artery. After 1, 3 and 5 days, 10 rats from the control group and 10 rats from each time group of the experimental group were sacrificed, and both left and right kidneys from each rat were removed separately.
Paraffin-embedded (5 μm thick) sections were deparaffinized and endogenous peroxidase was quenched with 3% (v/v) H2O2. The antigen retrieval was done by microwave at 600 power 10 min, in 0.1 M citric acid-sodium citrate buffer, pH 6.0. Non-specific binding was blocked using 5% (v/v) normal sheep serum for 30 min at 37°C. Sections were then incubated with primary antibodies (anti-EGFP 1:100, anti-DCN 1:200, anti-vimentin 1:50, anti-PCNA 1:100, anti-collagen IV 1:50) at 4°C overnight. Immobilized antibodies were detected by the avidin/biotin/peroxidase technique (Vectastain ABC Kits, Vector Laboratories, UK). Diaminobenzidine was used as the chromogen and hematoxylin was used as the nuclear counterstain. The primary antibody was replaced by PBS in the negative control.
We selected five different glomeruli from each slide at random for the evaluation of staining results. The positive area of each glomerulus was measured by computer-assisted image analysis (KS400, Zeiss, Germany). All values are expressed as mean ± SD. Results were analyzed by Student’s t-test with statistically significant difference set at P ≤0.05.
Results and discussion
It has been reported that the N/P ratio can influence the efficiency of nanoparticle transfection in vitro. To identify the optimal transfection conditions, pEGFP was used as a reporter and PEI– pEGFP nanocomplexes with different N/P ratios were prepared. The particle size and the zeta potential of PEI– pEGFP nanocomplexes were examined (Figure1). We found the diameter of the nanocomplex increased from N/P 1 to N/P 5 and then decreased from N/P 5 to N/P 20, while the zeta potential of nanocomplexes indicated positive charges increased with increasing N/P ratio, which is consistent with the report by Ogris. Quantitative analysis of transfection was done with an inverted fluorescence microscope after 48 h. The results showed that transfection efficiency increased with N/P ratios from 1– 20 and peaked at 20 with a smaller diameter, the expression of EGFP fluorescence was highest and nearly equal to that achieved by Lipofectamine™-mediated gene delivery (Figure2A). We used flow cytometry to further estimate the transfection efficiency and the result was the same as that described above (Figure2B). Moreover, the 3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test showed that cell viability was a little lower in cell groups of N/P 1– 20, but there was no significant difference in cell viability between the control cells and each group of transfected cells with N/P 1 – 20. However, cell viability was decreased significantly in the N/P 25 group. The group of Lipofectamine™-transfecting cell also had significant cytotoxicity (Figure2C). The PEI– pDNA complex at an N/P ratio of 20 has the highest transfection efficiency and the lowest cytotoxicity in cultured MC.
The deliverer for gene therapy generally has two forms, viral and nonviral. Currently, PEI has emerged as a potent nonviral vector for gene therapy in vitro and in vivo. The vehicle with efficient DNA condensation has been used successfully to transfer genes into living cells[25–27]. It has been shown that the N/P ratio influences the efficiency of the gene delivery system and cytotoxicity dramatically, and the optimal N/P ratio differs between cell lines[28, 29]. Therefore, it is important to select an optimal N/P ratio of a PEI nanoparticle. In this study, the PEI–pEGFP complexes showed the highest transfection efficiency and relatively lower cytotoxicity at an N/P ratio of 20.
To investigate the effect of PEI– pDCN on MC biology, we transfected a PEI– pDCN nanocomplex into cultured rat MC at N/P 20, which led to over-expression of DCN in MC (Figure3A). Western blotting (Figure3B) showed that the protein level of DCN in D6 increased significantly, whereas the expression of TGF-β1 and collagen IV decreased markedly compared to the control cells. The results suggest that over-expression of DCN could inhibit the expression of TGF-β1 and collagen IV of MC in vitro.
To further examine whether over-expression of DCN affects the growth of MC, cell cycle analysis of D6 cells and control cells was done by flow cytometry and the results showed that D6 cells in the G0/G1 and S phases were 84.47 ± 1.05% and 5.26 ± 0.51%, respectively. Compared to the controls, the difference was significant (Table1). These data indicate that over-expression of DCN could suppress cell growth by arresting MC in the G0/G1 phase.
We used pEGFP as a reporter gene and prepared stably transfected MC with PEI– pEGFP complexes to estimate the glomerular targeting of the MC vector. We injected MC vector into the normal rat kidney via the left renal artery and the rats were sacrificed after 2 days. Immunohistochemistry showed that about 65 ± 2.13% of the glomeruli in the left kidney were positive for EGFP staining and these cells were located mainly in the mesangium and the glomerular capillary (Figure4B), whereas the right kidney of the same animal was negative for EGFP and served as the control (Figure4A). Subsequently, the MC vector with PEI– DCN was also injected into the normal rats kidney. Immunohistochemical staining for exogenous DCN expressing cells had the same localization in glomeruli as EGFP (Figure4C), which is consistent with the distribution pattern of vimentin-positive cells that is recognized as a mesangial cell marker in glomeruli (Figure4D). By contrast, DCN staining was negative in the normal kidney where DCN was expressed at very low or even undetectable levels.
The rat anti-Thy1.1 glomerulonephritis model was induced by injection of rabbit anti-thymocyte serum into tail veins. The proliferation of MC and matrix in mesangium occurred 3 days after injection and more severe changes were seen at day 7.
Furthermore, the rat models were treated by MC (D6) vector with PEI– pDCN via injection of the left renal artery. Immunohistochemistry showed the DCN expression of D6 cells was located in glomeruli 1 day after the injection; its expression level was enhanced at day 3 and decreased slightly at day 5. However, the expression of DCN in all groups was significantly higher than that in the uninjected (right) kidney; DCN expression was totally absent from the uninjected control kidneys (Figure5A and B).
The expression of collagen type IV and PCNA in the glomeruli was detected by immunohistochemical staining and the results showed that the expression levels of both collagen type IV and PCNA in glomeruli in the PEI– pDCN group were decreased significantly compared to that in uninjected kidneys (Figure5A and B). Thus, matrix synthesis and cell proliferation were inhibited effectively by DCN over-expression in D6 cells.
Efficient targeted gene therapy in vivo is still a major challenge. PEI is one of the most efficient nonviral vectors but there are still many problems limiting its systemic application following intravenous administration in vivo. For example, the PEI–DNA complex is restricted by a shortage of organ targeting; it is usually expressed and aggregates in lung tissue via rat tail vein injection[32, 33]. Cationic polymers and DNA are known to bind serum proteins, which might serve to opsonize the complexes and perhaps promote rapid clearance by macrophages in the bloodstream[34–36]. Therefore, the recent trial of PEI delivery of genes to target cells in vivo were done mainly by direct injection either into the target tissue[13, 14, 37] or into its blood supply[38, 39] or by introduction with an appropriate cell vector.
PEI-mediated gene delivery to rat kidney was reported by Boletta et al.. The PEI– DNA particles injected into the left kidney via the left renal artery were localized exclusively in the proximal tubule, suggesting that direct injection with PEI– DNA complexes in kidney could be an effective route for renal tubulointerstitial injury instead of glomerular diseases. Furthermore, the ex vivo trial using a mesangial cell as a vector to transfer PEI– DNA is more successful for specific targeting of glomeruli. With the homing instinct of a cell vector, MC with β-galactosidase plasmid injected into the left kidney via the renal artery were located and enzyme expressed mainly in the glomeruli capillary and mesangium. This is a good example of attractive targeting strategy for transgene therapeutics of glomerular diseases. In this study, we transferred the MC vector with the PEI– DCN complex into the kidney of rat anti-Thy1.1 nephritis. The results showed that the exogenous MC with DCN expressing were located mainly in the mesangium and the glomerular capillary. Rat anti-Thy1.1 nephritis models are characterized by MC proliferation accompanied with ECM deposition. Over-expression of DCN in diseased glomeruli by injection of MC with a PEI– DCN complex could alleviate the pathological changes of rat nephritis. Thus, the DCN gene delivered by a PEI– DNA nanocomplex with the MC vector is proved to be a promising therapeutic reagent for the treatment of glomerulonephritis.
This study demonstrated that PEI-mediated DCN gene transfer to MC is effective and could be used as a gene-stable transfection carrier in vitro. We transferred a PEI–DCN complex into the glomeruli of a rat anti-Thy1.1 nephritis model by the MC vector with the PEI–DCN complex by injection into the left renal artery. We observed expression of the DCN protein in transfected MC in injected glomeruli, which have an important antagonistic effect on glomerular lesion of rat anti-Thy1.1 nephritis. We have shown the ex vivo gene transfer of a PEI–DCN complex to glomeruli is feasible and could be a useful method for gene therapy of glomerular diseases.
hemagglutinating virus of Japan
green fluorescent protein
anti-rat thymocyte serum.
Merdan T, Kopecek J, Kissel T: Prospects for cationic polymers in gene and oligonucleotide therapy against cancer. Adv Drug Deliv Rev 2002, 54: 715–758. 10.1016/S0169-409X(02)00046-7
Song S, Goudy K, Campbell-Thompson M, Wasserfall C, Scott-Jorgensen M, Wang J, Tang Q, Crawford JM, Ellis TM, Atkinson MA, Flotte TR: Recombinant adeno-associated virusmediated alpha-1 antitrypsin gene therapy prevents type I diabetes in NOD mice. Gene Ther 2004, 11: 181–186. 10.1038/sj.gt.3302156
Olefsky JM: Diabetes: Gene therapy for rats and mice. Nature 2000, 408: 420–421. 10.1038/35044177
Lungwitz U, Breunig M, Blunk T, Göpferich A: Polyethylenimine-based non-viral gene delivery systems. Eur J Pharm Biopharm 2005, 60: 247–266. 10.1016/j.ejpb.2004.11.011
Lemkine GF, Demeneix BA: Polyethylenimines for in vivo gene delivery. Curr Opin Mol Ther 2001, 3: 178–182.
Tang MX, Szoka FC: The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther 1997, 4: 823–832. 10.1038/sj.gt.3300454
Intra J, Salem AK: Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo. J Control Release 2008, 130: 129–138. 10.1016/j.jconrel.2008.04.014
Zhang XQ, Intra J, Salem AK: Comparative study of poly (lactic-co-glycolic acid)-polyethyleneimine-plasmid DNA microparticles prepared using double emulsion methods. J Microencapsul 2008, 25: 1–12. 10.1080/02652040701659347
Fischer D, Bieber T, Li Y, Elsässer HP, Kissel T: A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity. Pharm Res 1999, 16: 1273–1279. 10.1023/A:1014861900478
Huh SH, Do HJ, Lim HY, Kim DK, Choi SJ, Song H, Kim NH, Park JK, Chang WK, Chung HM, Kim JH: Optimization of 25 kDa linear polyethylenimine for efficient gene deliver. Biologicals 2007, 35: 165–171. 10.1016/j.biologicals.2006.08.004
Kichler A: Gene transfer with modified polyethylenimines. J Gene Med 2004, 6(Suppl 1):S3-S10.
Morille M, Passirani C, Vonarbourg A, Clavreul A, Benoit JP: Progress in developing cationic vectors for non-viral systemic gene therapy against cancer. Biomaterials 2008, 29: 3477–3496. 10.1016/j.biomaterials.2008.04.036
Aoki K, Furuhata S, Hatanaka K, Maeda M, Remy JS, Behr JP, Terada M, Yoshida T: Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity. Gene Ther 2001, 8: 508–514. 10.1038/sj.gt.3301435
Louis MH, Dutoit S, Denoux Y, Erbacher P, Deslandes E, Behr JP, Gauduchon P, Poulain L: Intraperitoneal linear polyethylenimine (L-PEI)-mediated gene delivery to ovarian carcinoma nodes in mice. Cancer Gene Ther 2006, 13: 367–374. 10.1038/sj.cgt.7700893
Di Gioia S, Conese M: Polyethylenimine-mediated gene delivery to the lung and therapeutic applications. Drug Des Devel Ther 2009, 2: 163–188.
Gautam A, Densmore CL, Waldrep JC: Inhibition of experimental lung metastasis by aerosol delivery of PEI-p53 complexes. Mol Ther 2000, 2: 318–323. 10.1006/mthe.2000.0138
Seong JH, Lee KM, Kim ST, Jin SE, Kim CK: Polyethylenimine-based antisense oligodeoxynucleotides of IL-4 suppress the production of IL-4 in a murine model of airway inflammation. J Gene Med 2006, 8: 314–323. 10.1002/jgm.848
Wu H, Wang S, Xue A, Liu Y, Liu Y, Wang H, Chen Q, Guo M, Zhang Z: Overexpression of decorin induces apoptosis and cell growth arrest in cultured rat mesangial cells in vitro. Nephrology 2008, 13: 607–615. 10.1111/j.1440-1797.2008.00961.x
Isaka Y, Brees DK, Ikegaya K, Kaneda Y, Imai E, Noble NA, Border WA: Gene therapy by skeletal muscle expression of decorin prevents fibrotic disease in rat kidney. Nat Med 1996, 2: 418–423. 10.1038/nm0496-418
Kim HJ, Kim SI, Yun IJ, Kwak JH, Yu SH: Gene delivery into rat glomerulus using a mesangial cell vector. Mol Cells 2000, 10: 662–668.
Zhang M, Guo MY, Chen Q, Jin HM: The culture of rat glomerular mesangial cells. Journal of Shanghai Medical University 1995, 22: 207–209. in Chinese in Chinese
Wang H, Zhang Z, Liu X, Chen G, Chen Qi-Guo M: Influence on the Growth and Changes of Several Phenotypes of Cultured Rat MsC Transfected by DCN Gene. Journal of Shanghai Medical University 2003, 30: 414–417. in Chinese in Chinese
Chen G, Guo M, Zhang Y, Zhang J, Zhang X, Chen Q: Preparation of anti-thy1 serum and establishment of rat mesangioproliferative glomerulonephritis model. Chinese Journal of Clinical and experimental Pathology 1996, 12: 241–245. in Chinese in Chinese
Ogris M, Steinlein P, Kursa M, Mechtler K, Kircheis R, Wagner E: The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther 1998, 5: 1425–1433. 10.1038/sj.gt.3300745
Boussif O, Lezoualc’h F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP: A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci 1995, 92: 7297–7301. 10.1073/pnas.92.16.7297
Aoki K, Furuhata S, Hatanaka K, Maeda M, Remy JS, Behr JP, Terada M, Yoshida T: Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity. Gene Ther 2001, 8: 508–514. 10.1038/sj.gt.3301435
Nimesh S, Chandra R: Polyethylenimine nanoparticles as an efficient in vitro siRNA delivery system. Eur J Pharm Biopharm 2009, 73: 43–49. 10.1016/j.ejpb.2009.04.001
Godbey WT, Wu KK, Mikos AG: Poly(ethylenimine)-mediated gene delivery affects endothelial cell function and viability. Biomaterials 2001, 22: 471–480. 10.1016/S0142-9612(00)00203-9
Gharwan H, Wightman L, Kircheis R, Wagner E, Zatloukal K: Nonviral gene transfer into fetal mouse livers (a comparison betneen cationic polymer PEI and naked DNA). Gene Ther 2003, 10: 810–817. 10.1038/sj.gt.3301954
Yuasa T, Izawa T, Kuwamura M, Yamate J: Thy-1 Expressing Mesenchymal Cells in Rat Nephrogenesis in Correlation with Cells Immunoreactive for α-Smooth Muscle Actin and Vimentin. J Toxicol Pathol 2010, 23: 1–10. 10.1293/tox.23.1
Kircheis R, Blessing T, Brunner S, Wightman L, Wagner E: Tumor targeting with surface-shielded ligand–polycation DNA complexes. J Control Release 2001, 72: 165–170. 10.1016/S0168-3659(01)00272-3
Bragonzi A, Boletta A, Biffi A, Muggia A, Sersale G, Cheng SH, Bordignon C, Assael BM, Conese M: Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs. Gene Ther 1999, 6: 1995–2004. 10.1038/sj.gt.3301039
Rudolph C, Lausier J, Naundorf S, Müller RH, Rosenecker J: In vivo gene delivery to the lung using polyethylenimine and fractured polyamidoamine dendrimers. J Gene Med 2000, 2: 269–278. 10.1002/1521-2254(200007/08)2:4<269::AID-JGM112>3.0.CO;2-F
Ogris M, Brunner S, Schuller S, Kircheis R, Wagner E: PEGylated DNA/transferring PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther 1999, 6: 595–605. 10.1038/sj.gt.3300900
Dash PR, Read ML, Fisher KD, Howard KA, Wolfert M, Oupicky D, Subr V, Strohalm J, Ulbrich K, Seymour LW: Decreased binding to proteins and cells of polymeric gene delivery vectors surface modified with a multivalent hydrophilic polymer and retargeting through attachment of transferrin. J Biol Chem 2000, 275: 3793–3802. 10.1074/jbc.275.6.3793
Kircheis R, Wightman L, Wagner E: Design and gene delivery activity of modified polyethylenimines. Adv Drug Deliv Rev 2001, 53: 341–358. 10.1016/S0169-409X(01)00202-2
Chen G, Zhang X, Yang F, Mu L: Disposition of nanoparticle-based delivery system via inner ear administration. Curr Drug Metab 2010, 11: 886–897. 10.2174/138920010794479673
Boletta A, Benigni A, Lutz J, Remuzzi G, Soria MR, Monaco L: Nonvival gene delivery to the rat kidney with polyethylenimine. Hum Gene Ther 1997, 8: 1243–1251. 10.1089/hum.1997.8.10-1243
Liu M, Li ZH, Xu FJ, Lai LH, Wang QQ, Tang GP, Yang WT: An oligopeptide ligand-mediated therapeutic gene nanocomplex for liver cancer-targeted therapy. Biomaterials 2012, 33: 2240–2250. 10.1016/j.biomaterials.2011.11.082
This project was supported by a grant from the National Natural Science Foundation of China (NSFC: 30570859). We thank Professor Muyi Guo and Dr Xiurong Zhang for advice and help with techniques, and Mr Huakang Cao for his kind instructions for animal experiments.
The authors declare that they have no competing interest.
JYS performed the experiments and drafted the manuscript. YS, HJW and HXZ carried out the partial experiments and participated in the mechanism analysis. ZHZ and QC helped in the technical support for the experiments. ZGZ designed the experiments and revised the manuscript. All authors read and approved the final manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Sun, JY., Sun, Y., Wu, HJ. et al. Transgene therapy for rat anti-Thy1.1 glomerulonephritis via mesangial cell vector with a polyethylenimine/decorin nanocomplex. Nanoscale Res Lett 7, 451 (2012). https://doi.org/10.1186/1556-276X-7-451
- Mesangial cell
- Rat anti-Thy1.1 nephritis