Outcomes and factors affecting mortality and successful tracing among patients lost to follow-up from antiretroviral therapy in Pawi Hospital, Northwest Ethiopia
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Loss to follow-up (LTFU) is a major public health problem to antiretroviral therapy (ART) programs in sub-Saharan Africa. Failure to account for patients’ LTFU outcomes (self-transfers and restarts) can result in inaccurate reporting of retention in care. In Ethiopia, specifically in the Benishangule Gumuz region, high LTFU reported patients, who were not assessed for their outcomes, are identified as a gap. Therefore, our objective was to determine the outcomes (alive or dead) of patients lost to follow-up (LTFU) from ART and identify factors associated with successful tracing and mortality of these patients.
The proportion of successful tracing was 75.5% (249 of 330). Among the traced patients (n = 249), 22.9% were deceased, 47.8% were on ART, and 29.3% had discontinued treatment. However, the remaining untraceable patients were not locatable due to wrong addresses (53.1%), change of residence (29.6%), and/or lack of functional phone contact (17.3%). Some (32.9%) of the patients discontinued because of negative test results, others (21.9%) for spiritual reasons or side effects (28.8%), and the remaining (16.4%) for other reasons. Tracing using phone numbers (AOR = 2.97, 95% CI 1.57–5.59) and existing long-term follow-up period for ART (AOR = 2.13, 95% CI 1.17–3.88) were strong predictors of successful tracing while not receiving cotrimoxazole preventive therapy (CPT) (AOR = 2.59, 95% CI 1.22–5.39) is a predictor for mortality of patients post-LTFU.
ART programs need to retain current contact information of patients or guardians/friends for tracing. Having phone contact numbers and prolonged lengths of compliance with ART are predictors of successful tracing, while lack of cotrimoxazole preventive therapy is a predictor of mortality. Early tracing of beginners (newly admitted recipients) and updating their detailed information at each follow-up visit is essential.
KeywordsAntiretroviral therapy Loss to follow-up Mortality ART tracing Cotrimoxazole preventive therapy Ethiopia
Adjusted odds ratio
Cotrimoxazole preventive therapy
Highly active antiretroviral therapy
Loss to follow-up
Pawi General Hospital
People living with human immunodeficiency virus
World Health Organization
Highly active antiretroviral therapy (HAART) has become widely available in low- and middle-income countries where human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS) were most prevalent. By the end of 2015, 17 million people globally had received ART, while at least 12 million people were living with HIV in sub-Saharan Africa .
Retention in care and support are important measures of ART program effectiveness and critical in achieving the 90-90-90 targets (90% of people living with human immunodeficiency virus (PLHIV) know their status, 90% of PLHIV who know their status are on treatment (ART), and 90% of PLHIV on treatment have attained viral suppression) [1, 2, 3]. “Loss to follow-up” is a general term for unknown outcomes of patients who have interrupted or not attend their ART medication follow-up visits. Studies in Ethiopia indicated a high proportion of loss to follow-up (LTFU), ranging between 14.5% and 22.6% for ART [4, 5, 6, 7, 8]. Outcomes for LTFU patients were classified into four categories: self-transfer, restart, discontinuance, and death [9, 10]. Therefore, it is possible to determine the actual outcomes of LTFU through active tracing by phone calls and field/home visits [11, 12, 13].
However, outcomes of post-LTFU patients have not been identified or described in the literature. Failure to account for patients’ outcomes (self-transfer and restart) can result in inaccurate reporting of retention in care, which is an essential indicator of measures of the success of ART scale-up as well as ensuring supply chain management of laboratory investigations, medications, and human resource requirements .
In Ethiopia, LTFU patients are not assessed for their outcomes; hence, this study aimed to determine outcomes of patients LTFU and identify factors associated with tracing and mortality of these patients. By assessing outcomes and factors affecting mortality and tracing will provide information for clinicians seeking appropriate management of patients on HAART. Moreover, policy makers and public health professionals will use the findings to design ART-related policies, programs, and services.
Methods and materials
We conducted a cross-sectional study at Pawi General Hospital (PGH). PGH is one of three (Assossa, Pawi, and Kamashi) hospital clinics that provide ART services in Benishangule Gumuz Region and is found in the Metekel zone, Northwest Ethiopia. The hospital has provided chronic HIV care and support to both pre-ART and ART clients since 2005. Between April 2007 and March 2017, 1760 patients were started on ART at this site.
The study population included all adult patients age 18 and older, who started ART at PGH and classified as LTFU. Identified LTFU patients and their baseline information were extracted from hospital records/charts retrospectively from 2007 to 2017. Lost to follow-up was considered for those who were not taking an ART refill for a period of 3 months and above. Finally, tracing was conducted from April 1 to June 30, 2017, and prevalence of tracing was determined at a given LTFU population. To check sample adequacy, a minimum sample size of 296 was determined based on single population proportion formula with consideration of a 10% non-response rate, and finally we surveyed all 330 patients in the given hospital.
When patients start ART, their baseline characteristics are entered on master cards, which are required at each monthly visit to the ART clinic to collect the next month’s supply of ART drugs. If a patient is not seen in the clinic for three consecutive months, the patient’s status is termed as “lost to follow-up” on the master card and the register book. We identified all adult patients listed as LTFU on the master cards and registers. We excluded patients whose contact address was outside Metekel zone, Benishangule Gumuz, Ethiopia and/or whose phone contact was not provided.
The operational definitions of LTFU was those who were not taking an ART refill for a period of 3 months or longer from the last attendance for refill. Transferred out reflected those patients who were formally transferred out to another health facility. Working functional status is defined as those patients who were able to perform their day-to-day activities. Negative test result refers to those patients who had started ART with a false positive test and then become negative or a negative test result with a false negative diagnosis but may actually be positive.
Data quality was maintained by a pre-tested data collection tool to collect relevant information on LTFU outcomes of current ART status and capturing LTFU elements such as restart at former clinic, self-transfer to other clinics discontinued, or deceased. Training was given for HIV-positive outreach tracers who visited patient homes and made phone calls between April 1 and June 30, 2017. Close supervision and verifications of data completeness were done. If patients were traced directly, they were asked whether they were still taking ART and, if not, why they discontinued. If a patient had died or moved away, the relatives were asked when the patient died or moved which was also entered into the data set.
Data management and statistical analysis
We used descriptive analysis to examine demographic and clinical characteristics as well as the history of LTFU patients and their outcomes. Outcomes were classified as alive, dead, or untraceable. Patients found alive were further categorized as restart, self-transferred out to another clinic, or discontinued. Logistic regression model was fitted to determine associated factors, and variables with p value below 0.25 in bivariate analysis were entered to multivariable analysis to adjust odds ratios that were mutually adjusted for all other predictor variables. The adjusted odds ratios with a p value ≤ 0.05 were considered statistically significant, and model fitness was assessed by Hosmer-Lemeshow goodness of fit test for logistic regression. The data was entered by Epi Info 7, and further analyses were done by using Stata/se Version 14 software.
There were 480 (27%) patients classified as LTFU from the pool of 1760 ART-initiated HIV-positive patients at Pawi hospital in the study period.
Socio-demographic characteristics of LTFU patients
Socio-demographic characteristics of patients included in the study
Sex of respondent
> 40 years
Secondary and above
Baseline clinical and related characteristics of LTFU patients
Baseline clinical and related characteristics of patients included in the study
Isoniazid preventive therapy (IPT)
Not received IPT
Duration of follow-up on ART
Follow-up time < 12 months
Follow-up time > 12 months
WHO clinical stage
No opportunistic infection
Successful tracing of LTFU patients
Three hundred and thirty of the LTFU patients were eligible for tracing of which 249 (75.45%) of the patients were successfully traced. Of these located LTFU patients, the status of 98 (39.4%) patients found at hospital traced records and the remaining 151 (60.6%) had to be traced by outreach. Tracing by field/home visit was done for 220 (66.7%) patients with 154 (61.8%) being located, while tracing via phone contact was undertaken for 110 (33.3%) with a successful contact with 95 (38.2%) patients.
Outcomes of LTFU patients
From the 249 LTFU patients who were successfully traced and evaluated for their LTFU outcomes, 192 (77.1%) were alive and 57 (22.9%) dead. Of those who were alive, 79 (41.2%) were on ART at Pawi Hospital, 40 (20.8%) at another ART clinic, and 73 (38%) had stopped ART. For 16 (21.9%) of those who stopped treatment, the rationale was due to a spiritual belief; for 21 (28.8%), side effects were the primary reason for discontinuance; 24 (32.9%) believed they were cured (negative test); and for 12 (16.4%), no reasons were specified. One fourth (24.5%) of the study population was not traced successfully due to wrong address, 43 (53.1%); change of residence, 24 (29.6%); and/or phone contact failure (17.3%).
Factors associated with successful tracing of LTFU patients
Factors associated with successful tracing of LTFU adult HIV-positive patients
Tracing N (%)
Crude OR (95% CI)
Adjusted OR (95% CI)
> 40 years
< 12 months
> 12 months
Factors associated with mortality of LTFU patients
Factors associated with mortality of adult HIV-positive patients LTFU
Outcomes number (%)
Crude OR (95% CI)
Adjusted OR (95% CI)
> 40 years
< 12 months
> 12 months
Exhaustive tracing studies of LTFU patients are important efforts to improve the quality of care as well as the outcome evaluation of ART programs in Ethiopia. Hence, this study was conducted to determine outcomes and factors affecting mortality and successful tracing among patients LTFU from ART. We achieved 75.5% successful tracing for LTFU patients which revealed mortality of 22.9%. In addition to deceased, live patients further identified as LTFU causes of 20.8% were self-transferred to other ART clinics, 41.2% were on ART at Pawi Hospital, and 38% had discontinued the treatment. Moreover, having a phone number and completing follow-up for more than a year were significantly associated with successful tracing whereas CPT was the only significant predictor of mortality.
The proportion of successful tracing was in line with previous studies conducted in Kenya  and Malawi  and a systematic review of studies in Africa . However, the findings in this study are higher than those of a systematic review in other resource-limited settings  which could reflect that most of the residents in the study area are difficult to trace due to issues in addressing. In our study, tracing via phone contacts and longer follow-up on ART were more successful in revealing the outcomes of LTFU. But, as in other studies, we failed to find any significant relations among age categories, baseline advanced clinical stage, functional status, CPT, and baseline CD4 cell count with tracing success [13, 16].
The availability of phone contacts appears an effective way of identifying outcomes of LTFU patients. Via telephone, patients were more likely to be located on a first attempt, to admit leaving the clinic, and to reveal their outcome status. Likewise, LTFU survivors are less likely to change their personal or friends’ phone numbers leading to successful phone tracing. Both scenarios could decrease the proportion of tracing patients via in-person field tracing compared to phone calls as observed in our study and mirrored in a study in Malawi . Cell phone coverage is increasing the positive experience of using phones in patient follow-up, resulting in more successful tracing . However, this study conflicts with findings from a studies in South Africa [17, 19], although its confidence interval was very wide (95% CI 1.5–47.7), indicating a large variability and weak evidence.
Longer length of time attending ART before stopping was a predictor for successful tracing. Longer length of time attending ART may increase patients’ familiarity with all service providers in terms the details of their personal biography, which minimizes incomplete patients’ contact details.
This study also indicated that 23% of the patients traced had died, which is similar to the findings in a Kenyan study  which could be due to relatively similar settings in Ethiopia. However, it is lower than studies in Malawi and other select resource-limited settings of sub-Saharan Africa [12, 17, 20], which may be attributable to variations in study settings and periods.
These findings suggest that CPT is a strong predictor of mortality among traced patients that missed scheduled visit dates. Adults who did not receive CPT had significantly higher incidences of mortality as end points. This finding could be due to LTFU patients also choosing to discontinue CPT despite its potential to reduce mortality by reducing comorbidities during the LTFU period [9, 18, 21, 22]. In our study, advanced age, advanced WHO stages, and lower baseline CD4 counts were not significantly predictive for patients who died.
Limitation of the study
One limitation of this work is excluding patients who have no phone contact and home address outside of the Metekel zone due to lack of resources and technology. In addition, dependence on patient’s or family/friends’ recall of the details of the ART services they have received, or their tendency to report outcomes as they feel desirable or acceptable to the researchers/tracers is also a limitation. This inaccurate/misgiven information could bias reporting of the outcomes of patients LTFU.
ART programs need to retain current contact information of patients or family/friends for tracing. Having phone contact numbers and prolonged lengths of compliance with ART are predictors of successful tracing, while lack of CPT is a predictor of mortality. Early tracing of beginners (newly admitted recipients) and updating their detailed information at each follow-up visit is essential.
The authors would like to express their deepest gratitude to patients who participated in the study and to the data collectors. We would also like to extend our gratitude to Pawi General Hospital administration for their cooperation.
MAA contributed to the conception of research protocol, study design, data analysis, interpretation, and drafting the manuscript. CTL facilitated the data collection and management and drafted and critically reviewed the manuscript. PMP critically reviewed, edited, and validated the research and manuscript. All authors read and approved of the final manuscript.
Ethics approval and consent to participate
Ethical clearance was obtained from the College of Health Science Institutional Review Board, Debre Markos University. Written informed consent was obtained from individuals without waiver by creating public awareness before the commencement of the study as approved by IRB (Ref. No. 640/11/17). General measures were provided in ART facilities to ensure patient confidentiality. The data collected did not use their personal identifiers. Data were anonymized, and confidentiality was maintained during all phases of the research activities.
Consent for publication
The authors declare that they have no competing interests.
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