Prevalence of Blastocystis sp. infection in several hosts in Brazil: a systematic review and meta-analysis



Blastocystis sp. affects a wide variety of animals and is the most common protozoan in human fecal samples with potential pandemic distribution. In the present study, a systematic review and meta-analysis were conducted to determine the prevalence and distribution of Blastocystis sp. in different classes of hosts in Brazil.


Studies that analyzed hosts of various classes, including humans, domestic animals, wild animals or captive animals, were considered. The pooled prevalence of Blastocystis sp. infection was estimated by random effects models.


For humans, similar prevalence rates were found for males (31.0%, 95% CI: 17.0–45.0%; weight 10%) and females (28.0%, 95% CI: 16.0–41.0%; weight 10%); the state of Mato Grosso do Sul showed the highest prevalence, with 41.0% positivity (95% CI: 36.0–46.0%; weight 2.9%). The prevalence among immunocompromised patients was 5.0% (95% CI: 3.0–7.0%; weight 10%), and the most common cause of immunosuppression was hemodialysis, with 23.0% (95% CI: 17.0–29.0%; weight 12.4%). Among classifications according to interaction with humans, wild and domestic animals presented values of 19.0% (95% CI: 7.0–31.0%; weight 42.6%) and 17.0% (95% CI: 13.0–21.0%; weight 29.6%), respectively. Among these animals, mammals (39.0%, 95% CI: 21.0–56.0%; weight 47.3%) and birds (18.0%, 95% CI: 10.0–27.0%; weight 39.3%) exhibited the highest prevalence. Phylogenetic analysis of Blastocystis sp. revealed greater genetic diversity for clades of subtypes (STs) ST1, ST2 and ST3.


The overall prevalence of Blastocystis sp. in the Brazilian human population was 24%, which reflects the reality in the South, Southeast and Midwest regions, where prevalence rates of up to 40% were found. Among animals, mammals and birds exhibited the highest prevalence.


Blastocystis sp. is a protozoan parasite with no flagella belonging to class Blastocystea. This protozoan is commonly found in the gastrointestinal tracts of humans in addition to a wide variety of animals of various classes [1, 2].

Transmission of Blastocystis sp. occurs through the fecal-oral route [3]. Several studies suggest that contamination of water with fecal matter may be a source of infection [4,5,6]. For this reason, this parasite was included in water sanitation programmes and the Health Programme of the World Health Organization (WHO) [7].

Blastocystis sp. has been described as the most common eukaryotic organism in human fecal samples. It has a potential pandemic distribution, presenting prevalence rates that vary widely between countries and even between regions of the same country, reaching 30% in developed countries and up to 76% in developing countries [8,9,10,11,12,13].

There is an ongoing debate about the commensal or pathogenic nature of Blastocystis sp. In recent decades, several epidemiological studies have shown in vitro evidence in animal models and in humans strongly suggesting the pathogenic potential of this parasite [14,15,16,17,18,19]. Correlations between pathogenicity and subtypes (STs) of Blastocystis sp. have also been the subject of research, and results have indicated that not all strains of a given subtype are pathogenic. This fact suggests that subtype is not the only factor related to the pathogenicity of this parasite [15, 16]. Furthermore, not all humans are susceptible to infections caused by Blastocystis sp., which can be detected in asymptomatic hosts [1]. Regardless, there is a growing recognition of the pathogenicity of Blastocystis sp. in humans, even though its virulence mechanisms are not well described, because the symptoms of infection by this parasite are associated with non-specific gastrointestinal symptoms such as diarrhea, nausea, vomiting, abdominal pain and irritable bowel syndrome [20, 21].

Blastocystis sp. is commonly reported as a clinically relevant infection among immunosuppressed individuals that can result in severe diarrhea due to the progressive decline in defense mechanisms of these patients. Data on the prevalence of this pathogen indicate rates ranging from 15 to 25% in hemodialysis patients and from 20 to 35% in renal transplant patients [22,23,24,25,26].

Regarding Blastocystis sp. STs, analyses of the small subunit of the ribosomal RNA gene (SSU rDNA) have revealed genetic diversity represented by 17 genetically distinct strains (ST1-ST17) [27, 28]. To date, 10 subtypes have been found in humans (ST1-ST9 and ST12); however, 90–95% of human infections can be attributed to one of the ST1-ST4, with a predominance of ST3 [29,30,31,32,33]. All subtypes found in humans, except for ST9, have also been identified in animals, including non-human primates, mammals and birds [34, 35]. In Brazil, the subtypes found in animal hosts, including domestic, wild and captive, were ST1-ST5 and ST8 among mammals and birds [36, 37], indicating the potential for zoonotic transmission.

ST10 to ST17 have been found exclusively in animal hosts, non-human primates and other mammals [1, 27, 31, 38, 39]. Potential STs in non-mammal and non-avian hosts, so-called NMASTs (non-mammal and non-avian STs), have also been proposed for amphibians, reptiles and insects [39, 40].

Although there are data regarding the prevalence of Blastocystis sp. in some regions, no analysis of pooled prevalence and distribution according to STs by geographical area, type of host, sex and immunosuppression in Brazil has been published to date. This pioneering systematic review aimed to understand the prevalence and distribution of Blastocystis sp. in different classes of hosts in Brazil.


The protocol for this systematic review was published in the International Prospective Register of Systematic Reviews (PROSPERO 2018: CRD42018116792) before its implementation, as described in Additional file 1: Text S1. The protocol and final report were developed based on the Cochrane Handbook for Systematic Reviews of Interventions [41].

Review question

What is the prevalence, geographical distribution and phylogenetic relationships of Blastocystis sp. subtypes parasitizing different host species in Brazil?

Inclusion criteria

This review considered studies conducted with several hosts of various classes, including humans and domestic, wild and captive animals, in Brazil to determine the prevalence and molecular identification of Blastocystis sp. subtypes through coprological analyses and molecular techniques, excluding those that did not report a positivity percentage.

Search strategy

An initial search limited to MEDLINE was performed using MeSH index terms and related keywords. The search was followed by an analysis of the text of the title, abstract and index terms used to describe the article. A second search using all identified keywords and index terms was performed in all included databases. As a source of gray literature, a search was then performed in reference lists for dissertations that evaluated the prevalence of Blastocystis sp. Because this study focused on Brazil, the search was limited to articles published in English, Spanish and Portuguese. The search had no limits regarding the start date and was concluded in February 2019.

Studies were searched in the following databases: the Spanish Bibliographic Index of the Health Sciences (IBECS), the Latin American and Caribbean Health Sciences Literature (LILACS), the United States National Library of Medicine bibliographic database (Medline), the Elsevier database (EMBASE), the Cochrane Library, and National Institute for Health and Clinical Excellence (NICE). The MeSH index terms searched were Blastocystis, Blastocystis infections, Brazil, prevalence, and parasitology. The search terms are provided in Additional file 1: Text S1.

Evaluation of methodological quality

The articles selected for data retrieval were analyzed by two independent reviewers to evaluate the methodological validity of each paper before being included in this review. We evaluated the quality of the included publications based on criteria from the Grading of Recommendations Assessment, Development and Evaluation (GRADE) method. The studies received one point if they did not have limitations in study design or execution (risk of bias), inconsistency of results, indirectness of evidence, imprecision and publication bias. A score of four to five points was considered high quality, three points was considered moderate quality, and two to zero points was considered low quality.

Data extraction

The data were entered in Review Manager (RevMan 5.3) for analysis. A data extraction table was designed to evaluate the quality of the demographic data, study location, sample size, number of cases, number of positives and diagnostic test.

Data synthesis

The meta-analysis random-effect model was applied to analyze the pooled prevalence, with a 95% confidence interval (CI), of Blastocystis sp. infection in both humans and animals. Heterogeneity among the studies was analyzed using the Higgins test (I2), which describes the percentage of total variation across studies that is due to heterogeneity rather than chance. Analyses were performed using Stata v. 13.1.

Phylogenetic analysis

Partial sequences of the SSU rDNA gene from various Blastocystis sp. subtypes (ST1-ST5, ST8, ST11, ST12 and ST14) retrieved from the GenBank database were analyzed. Of the subtypes described in Brazil, only ST6 and ST7 were not included in the analysis because sequences of the SSU rDNA gene fragment were not available for these subtypes. The sequences were aligned in Clustal X software [42], with changes to the parameters related to the insertion of “Gaps” (insertion penalty = 1, extension penalty = 1). Phylogenetic inference was performed using the maximum likelihood (ML) method [43], with 500 replicates using the General Time Reversible (GTR) as the substitution model and four gamma categories and diagrams obtained by Maximum Likelihood (ML) as initial trees. The substitution model parameters employed were estimated during the search. Branch support was estimated using 500 bootstrap replicates in RAxML software.


Our study retrieved 1740 manuscripts using the search strategies employed. After the eligibility criteria were applied (duplicate texts, articles related to other topics, text excluded based on the review or methodological quality criteria), 40 studies were retained for analysis (Table 1) [9, 36, 37, 44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80]. Of these 40 studies, 35 evaluated the prevalence of Blastocystis sp. in fecal samples of humans from different Brazilian states and in distinct time periods; the other five studies evaluated the prevalence of Blastocystis sp. infection in wild, captive and domestic animals. Ten of the 40 studies provided a molecular characterization of Blastocystis sp. subtypes by SSU rDNA partial sequencing. The results of the search strategy are shown in a PRISMA flowchart (Fig. 1). The data extracted from the final selection are provided in Additional file 2: Table S1.

Table 1 A summary of the included studies
Fig. 1

A flowchart of the steps performed in the systematic review

Among the 35 studies utilizing human samples, 34 had high methodological quality, with a score of five. Only one study had a score of two; this study showed a risk of bias, imprecision and bias publication (small sample size). Publication bias was not assessed because currently available methods are not considered useful in studies on proportions. The five studies that evaluated the prevalence of Blastocystis sp. in different animal species (wild, captive and domestic animals) also showed high methodological quality, with a score of five. The I2 test indicated low heterogeneity among the studies. The summaries of methodological quality and bias risk and applicability for each study and among the included studies are shown in Additional file 3: Figure S1 and Additional file 4: Figure S2.

Blastocystis sp. in the human population of Brazil

For the 35 studies that included human samples, 14,917 coprological tests were performed, including samples from patients from different Brazilian states. Regarding the distribution of tests performed according to the states studied, five studies were performed in the state of Rio de Janeiro, representing 26.4% of the study samples, three studies in Minas Gerais (18.6%), 13 in São Paulo (17.5%) and six in Paraná (11.2%). Only one study each was conducted in the states of Goiás, representing 4.8% of the samples included, Mato Grosso (2.6%), Amazonas (2.4%), Mato Grosso do Sul (2.1%), Santa Catarina (1.3%), Rio Grande do Sul (0.7%) and Bahia (0.7%). Finally, one study analyzed patient samples from the states of São Paulo, Minas Gerais and Ceará, which represented 11.8% of the samples included in this meta-analysis.

Of the 35 studies analyzed, only 15 classified patient samples by sex, totaling 7948 samples (51.5% female and 48.5% male). Of these, only eight reported the distribution of positive tests according to sex in 2662 samples analyzed, with 1233 (43.7%) males and 1429 (56.3%) females.

Regarding the health status of the immune system, 11,503 (81.3%) samples were from patients without a previously reported compromised immune system; the remaining 2648 (18.7%) samples were from immunocompromised patients. The types of immunosuppression reported were organ transplantation (66.2%), use of immunosuppressive drugs (14.8%), human immunodeficiency virus (HIV) carriers (11.5%) and hemodialysis (7.4%).

Pooled prevalence of Blastocystis sp.

The prevalence of Blastocystis sp. infection reported in the studies analyzed ranged from 0.30% to 83.4%. One study included only one patient who was positive for this parasite. When the meta-analysis was performed using a random-effect model, we found an estimated pooled prevalence for Blastocystis sp. infection in the general Brazilian population of 24.0% (95% CI: 22.0–27.0%; weight 100%) (Fig. 2).

Fig. 2

Forest plot for a random-effect meta-analysis of Blastocystis sp. infection in the Brazilian population

When the pooled prevalence was analyzed per state, we found a prevalence of 41.0% for Mato Grosso do Sul, 40.0% for Rio Grande do Sul, 33.0% for São Paulo, 29.0% for Paraná, 23.0% for Rio de Janeiro, 20.0% for Minas Gerais, 17.0% for Mato Grosso, 13.0% for Bahia, 0% for Amazonas, 0% for Goiás, and 0% for Santa Catarina. Finally, the study that analyzed samples from São Paulo, Minhas Gerais and Ceará showed a pooled prevalence of 1.0% (Fig. 2). The pooled prevalence with complete 95% CI values for each state is shown in Table 2.

Table 2 Distribution of the pooled prevalence of Blastocystis sp. infection according to state and sex

The pooled prevalence calculated for the 1233 male samples was 31.0% (95% CI: 17.0–45.0%; weight 100%); the state with the highest prevalence was Rio de Janeiro, (63.0%), followed by Mato Grosso do Sul (36.0%); Paraná (28.0%); Mato Grosso (20.0%); Bahia (19.0%;) and São Paulo (7.0%). In turn, the pooled prevalence calculated for the 1429 female samples was 28.0% (95% CI: 16.0–41.0%; weight 100%); the state with the highest prevalence was Rio de Janeiro (48.0%), followed by Mato Grosso do Sul (47.0%), Paraná (27.0%), Mato Grosso (15.0%), Bahia (7.0%) and São Paulo (6.0%). The pooled prevalence with complete 95% CI values for each state by sex is shown in Table 2.

Among patients without a compromised immune system, the pooled prevalence was 29.0% (95% CI: 24.0–33.0%; weight 100%), whereas the pooled prevalence for immunosuppressed patients was 5.0% (95% CI: 3.0–7.0%; weight 100%). The cause of immunosuppression most prevalent with Blastocystis sp. infection was hemodialysis, at 23.0%, followed by HIV infection at 5.0%, organ transplant at 1.0% and immunosuppressive drug use at 1.0%. The pooled prevalence with complete 95% CI values for each type of immunosuppression is shown in Table 3.

Table 3 Distribution of the pooled prevalence of Blastocystis sp. infection according to the type of immunosuppression

Blastocystis sp. in animals from Brazil

In the five studies that analyzed the prevalence of Blastocystis sp. in animals in Brazil, 892 coprological tests were performed on different species of mammals, birds and reptiles. Regarding the classification of these animals, 65.0% were birds, 20.0% were mammals, and 15.0% were reptiles. Regarding classification according to their direct interaction with humans, 42.3% were wild, 37.4% were domestic, and 20.3% were in captivity.

After analyzing the infection by Blastocystis sp. in animals in Brazil of different orders and with different types of interaction with humans, a pooled prevalence of 21.0% (95% CI: 12.0–37.0%; weight 100%) was observed. The prevalence of Blastocystis sp. according to taxonomic class showed the highest percentage of infection among mammals, at 39.0%, followed by birds (18.0%) and reptiles (3.0%). Captive animals represented 23.0%, followed by wild animals at 19.0% and domestic animals at 17.0% (Fig. 3).

Fig. 3

Forest plot for a random-effect meta-analysis of Blastocystis sp. infection in different taxonomic classes of animals in Brazil, according to the type of interaction with humans

Among the mammals in captivity, non-human primates were the most studied, with high prevalence rates among Macaca mulata (60.0%) and Macaca fascicularis (35.0%). Didelphis aurita was the wild mammal with the highest prevalence (76.0%). Finally, among domestic mammals, Sus scrofa represented 24.0% and Bos taurus 21.0%. Notably, the only animals considered pets included in the analyzed studies, Canis lupus familiaris and Felis catus, had pooled prevalences of 3.0% and 0%, respectively.

Among birds, only species with domestic and wild interactions were studied. Anser anser showed a prevalence of 70.0% and Anas platyrhynchos domesticus a prevalence of 23.0%. Finally, wild birds positive for Blastocystis sp. were Penelope obscura, with a prevalence of 4.0%, and Oryzoborus angolensis, with a prevalence of 6.0%. The Chelonoidis sp., the only reptile species with captive interaction, showed a prevalence of 69.9% (95% CI: 42.0–87.0%; weight 5.10%). Finally, the cockroach Periplaneta americana showed a prevalence of 2.0% (95% CI: 1.0–7.0%; weight 7.54%). The pooled prevalence with complete 95% CI values for each taxonomic class and species of animal are shown in Table 4.

Table 4 Distribution of the pooled prevalence of Blastocystis sp. according to taxonomic class and species

Diversity of Blastocystis sp. subtypes in different host species in Brazil

SSU rDNA was used in 10 studies to identify Blastocystis sp. subtypes in samples from different host species. Of these studies, eight were performed in humans and two in animals of different classes. In total, 473 samples from humans and 118 from animals were studied.

The Blastocystis subtypes identified in the different hosts were ST1 to ST8, with the most prevalent being ST1, ST2 and ST3, with an overall percentage of infection of 86.2% (Fig. 4).

Fig. 4

Geographical distribution of Blastocystis subtypes detected in Brazil. a Subtypes detected in 473 human and 118 animal samples. b Distribution of subtypes in Brazilian states. In the state of Rio de Janeiro, all STs found in human samples were also found in animal samples. Abbreviations: MT, Mato Grosso; SP, São Paulo; RJ, Rio de Janeiro; PR, Paraná; MG, Minas Gerais

Although ST1 was more prevalent in humans (36.8%), it was also detected in domestic and wild mammals and birds (19.2%). In human hosts, ST1 was detected in the states of Mato Grosso, São Paulo, Paraná and Rio de Janeiro. In pigs, ST1 was identified in the state of Minas Gerais; in Rio de Janeiro, it was detected in non-human primates, marsupials, wild boars and birds.

ST2 and ST3 were detected in human samples from the states of Mato Grosso, São Paulo, Paraná and Rio de Janeiro. Both were found in samples from non-human primates in the state of Rio de Janeiro, and ST3 was also detected in a rodent in the same state (Fig. 4).

ST4 was the least prevalent (1.8%) and was found in human hosts in the states of Paraná and Rio de Janeiro. This subtype was also found in wild boar and cockroach samples and was the only subtype detected in insects in Brazil. ST5 was found only in rooster and wild boar samples in the state of Rio de Janeiro. ST6 and ST7 were identified only in human hosts in the states of São Paulo and Paraná. ST8 was identified in humans, non-human primates, marsupials, armadillo and wild boars in the states of Rio de Janeiro and Paraná (Fig. 4).

Phylogenetic analysis of Blastocystis sp. subtypes found in Brazil

To understand phylogenetic relationships between the subtypes and their interactions with their hosts, a phylogenetic analysis was performed using the ML estimation method, and 255 sequences of ST1-ST5, ST8, ST11, ST12 and ST14 were included. The accession numbers for the GenBank sequences are provided in Additional file 5: Table S2.

Of the subtypes found in Brazil (ST1 to ST8), only ST6 and ST7 were not included due to a lack of sequences compatible with the SSU rDNA gene fragment used to perform the alignment. The alignment used to perform the phylogenetic inference is provided in Additional file 6: Text S2.

The unrooted tree presents nine clades that correspond exactly to each ST included in the analysis (Fig. 5). Each subtype was strongly supported by a high bootstrap value. Furthermore, the results showed a relationship between clades ST1 and ST2, among clades ST5, ST12 and ST14, and between clades ST4 and ST8. Clades ST1, ST2 and ST3 exhibited greater genetic diversity because they have at least two possible genotypes in each ST. With the exception of ST11, of which only one sequence was included, the other clades included in the inference were shown to be genetically homogeneous (Fig. 5).

Fig. 5

A dendrogram inferred by Maximum Likelihood analysis using 255 sequences of the SSU rRNA gene fragment (365 characters, see alignment in Additional file 6: Text S2). Bootstrap node support values ≥ 0.95 are shown as circles at the nodes, and bootstrap node support values of 0.75–0.94 are shown as squares at the nodes


A pooled prevalence for Blastocystis sp. infection in the general Brazilian population of 24% was found in this meta-analysis. Of the five Brazilian regions, the highest amount of published data was for the South, South-East and Center-West regions. A total of 32 articles representing approximately 85.1% of the samples analyzed in this meta-analysis were included in these regions. Regarding the North and North-East regions, only one study per region was included, representing 3.1% of the samples analyzed. Notably, one study used samples from the North-East and South-East regions (São Paulo, Minas Gerais and Ceará), but the results were not segregated by state. This study included 11.8% of the samples analyzed. Regarding sex, both showed similar prevalence rates: 31% for men and 28% for women.

There are contrasting realities within the states that compose each region. In the Center-West, we observed high prevalence rates in the states of Mato Grosso do Sul (41.0%) and Mato Grosso (17.0%), yet the pooled prevalence in Goiás was 0%. The same phenomenon was observed in the South and South-East regions, where states such as Rio Grande do Sul (40.0%), São Paulo (33.0%), Paraná (29.0%) and Minas Gerais (20.0%) had high prevalence rates, but the same was not true for Santa Catarina (0%). Importantly, a significant number of samples was analyzed in these studies conducted in both Goiás and Santa Catarina, decreasing the probability of sampling error. However, in the few studies conducted in the states of Amazonas and Bahia, which are the only representatives of the North and North-East regions, the calculated prevalence rates were 0% and 13.0%, respectively. Regardless, further studies should be conducted to corroborate these rates in these regions.

Brazil is a country with many regional differences in climatic conditions and socioeconomic development. According to data from the Ministry of Cities (Sistema Nacional de Informações sobre Saneamento (National Sanitation Information System 2014, SNIS) [81], only 39% of the municipalities collect and treat 100% of their sewage. The lack of an appropriate waste collection and sewage treatment system can contribute to the dissemination of neglected diseases, including those caused by Blastocystis sp.

The prevalence of Blastocystis sp. in immunocompromised patients was 5.0% (95% CI: 3.0–7.0%; weight 100%). Patients under hemodialysis treatment were the most susceptible to infection by this parasite, at 23.0% (95% CI: 17.0–29.0%; weight 12.36%). HIV patients ranked second, with a prevalence of 5.0% (95% CI: 5.0–8.0%; weight 33.61%).

Some studies indicate that this parasite often causes opportunistic infection in immunosuppressed patients [65], specifically diarrhea that is usually accompanied by weight loss, vomiting, malabsorption syndrome and, in some cases, fever and abdominal pain [82]. Other studies have reported that Blastocystis sp. is responsible for clinically relevant infections in patients undergoing hemodialysis and kidney transplantation; the prevalence rates in these studies vary between 15–25% and 20–35%, respectively [22, 23, 25, 26]. Our results showed low prevalence rates in patients with organ transplants. As patients undergoing hemodialysis are candidates for kidney transplantation, planning strategies for the prevention of parasitic infections and appropriate interventions are necessary to improve the quality of life of these patients.

Furthermore, our results revealed a wide diversity of hosts, including animals of various orders (mammals, birds and reptiles) and even insects (cockroaches) capable of harboring and distributing Blastocystis sp. Among these animals, mammals showed the highest prevalence rates, at 39.0% (95% CI: 21.0–56.0%; weight 47.31%), followed by birds at 18.0% (95% CI: 10.0–27.0%; weight 39.31%). In regard to interaction with humans, the highest prevalence rates were observed for captive animals, which are not easily accessible to the population. Wild animals and domestic animals had the second highest prevalence rates. Although domestic animals had lower prevalence rates, a prevalence of 17.0% (95% CI: 13.0–21.0%; weight 29.64%), which is still significant, was observed. When domestic animals were analyzed, we found them to be animals related to livestock production. Among these animals, the wild boar (Sus scrofa) and the ox (Bos taurus) among mammals and the Greylag goose (Anser anser) and the domestic duck (Anas platyrhynchos domesticus) among birds had the highest prevalence rates. Notably, the prevalence rate among animals considered to be pets (cat and dog) was low. Thus, our results point to rearing livestock as a possible risk factor for Blastocystis sp., and control measures against intestinal parasites should be reinforced to minimize the transmission of these parasites through the feces of these animals.

Our phylogenetic inference revealed a relationship between ST1 and ST2 as well as between ST8 and ST4. Such a relationship was also proposed in a recent study that analyzed SSU rDNA sequences in fecal samples from animals of various orders [36]. After analyzing sequences from several STs in fecal samples from humans and other mammals, birds, reptiles and cockroaches, we observed a considerable overlap between different hosts and the Blastocystis subtypes. Therefore, no specific host-ST relationship could be detected. Because the ST2 sequences analyzed were from humans and non-human captive primates, it seems reasonable to assume the possibility of zoonotic transmission where these animals are kept. Other possible interactions may occur through contacts with domestic animals, especially on farms (ST1 and ST4).

The correlation between pathogenicity and Blastocystis sp. STs has been the subject of several studies, showing that not all strains of a subtype are pathogenic and suggesting that subtype is not the only factor related to pathogenicity [15, 16]. Indeed, our phylogenetic analysis suggests the possibility that more than one strain of ST1, ST2 and ST3 may result in different clinical symptoms in infected patients.

There are some limitations to our study. First, in the studies conducted in humans, some authors did not segregate the results of positive samples by sex, which decreased the number of samples available to evaluate prevalence for this variable. Secondly, the samples per state were not segregated in one study [59], but this may contribute to a better calculation of the prevalence rates in the states involved. Thirdly, our results only reflect the reality in the South, South-East and Center-West regions, which have higher scientific production. Fourthly, the lack of partial SSU rDNA sequences available for ST6, ST7, ST9, ST10, ST13, ST15, ST16 and ST17 led to the need to exclude these STs, limiting the phylogenetic analysis. Finally, in meta-analyses, it is recommended that publication bias be assessed through statistical methods. However, currently available methods such as funnel plots and Eggerʼs regression test are not considered useful in studies on proportions [83]. Additionally, the statistical power of these tests is affected by the presence of high heterogeneity and the limited number of studies [84]. Accordingly, publication bias was not measured.


This study revealed a high prevalence (24%) of Blastocystis sp. in the Brazilian population, a value that was influenced by the most studied regions (South, South-East and Center-West), where prevalence rates of up to 40% were found. Among animals, mammals and birds exhibited the highest prevalence rates, and domestic animals used as livestock are possibly most related to parasite transmission. Eco-epidemiological studies of Blastocystis sp. are very important due to the possible interactions of host animals with humans. Therefore, control measures against intestinal parasites should be reinforced to prevent the transmission of these parasites, principally in zoos and on farms. Finally, in patients with any type of immunosuppression, routine screening of opportunistic intestinal protozoa should be performed, and early treatment should be administered.

Availability of data and materials

All data are presented in the manuscript and its additional files.





ribonucleic acid


small subunit


ribosomal deoxyribonucleic acid


non-mammal and avian host STs


Prospective Register of Systematic Reviews


Medical Subject Headings


The Spanish Bibliographic Index of the Health Sciences


The Latin American and Caribbean Health Sciences Literature


The United States National Library of Medicine Bibliographic Database


The Elsevier database

The Cochrane Library and NICE:

National Institute for Health and Clinical Excellence


grading of recommendations assessment development and evaluation


review manager

I2 :

the Higgins test


maximum likelihood


general time reversible


preferred reporting items for systematic reviews and meta-analyses


human immunodeficiency virus


confidence interval


Mato Grosso


São Paulo


Rio de Janeiro




Minas Gerais


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The authors thank Universidade do Estado de Mato Grosso (UNEMAT). ADSZ has a fellowship sponsored by this institution.


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ADSZ, AFM, TAM and LMM analyzed and interpreted the study data and prepared the preliminary versions of the manuscript. FGL, SLS and SKIC devised the search strategies and analyzed the data. SMBF and EI analyzed the data and conducted the content review. ASZ, AFM and OAE designed and delineated the study, revised the manuscript content, and approved the final version to be published. All authors read and approved the final manuscript.

Correspondence to Omar Ariel Espinosa.

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Zanetti, A.d., Malheiros, A.F., de Matos, T.A. et al. Prevalence of Blastocystis sp. infection in several hosts in Brazil: a systematic review and meta-analysis. Parasites Vectors 13, 30 (2020).

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  • Blastocystis
  • Blastocystis infection
  • Meta-analysis
  • Brazil
  • Systematic review