Aberrant DNA methylation suppresses expression of estrogen receptor 1 (ESR1) in ovarian endometrioma
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In ovarian endometriomas (OE), the expression statuses of various steroid hormone receptors are altered compared with their expression statuses in eutopic endometrium (EE). For example, in OE, the expressions of estrogen receptor 1 (ESR1), which encodes ERα, and progesterone receptor (PGR) are downregulated, while the expression of ESR2, which encodes ERβ, is upregulated. The causes of these changes are unclear. DNA methylation of a specific region of a gene can result in tissue-specific gene expression. Such regions are called tissue-dependent and differentially methylated regions (T-DMRs). We previously reported that the tissue-specific expression of ESR1 is regulated by DNA methylation of a T-DMR in normal tissues. In the present study, we examined whether aberrant DNA methylation of the T-DMR is associated with the altered expressions of ESR1, ESR2 and PGR in OE.
Gene expression levels of ESR1, ESR2 and PGR were measured by quantitative RT-PCR. The expression levels of ESR1 and PGR were significantly lower and the expression level of ESR2 was significantly higher in OE than in EE. DNA methylation statuses were examined with an Infinium HumanMethylation450K BeadChip and sodium bisulfite sequencing. DNA methylation at the T-DMRs of ESR1 were significantly higher in OE than in EE, but no significant differences were observed in the DNA methylation statuses of ESR2 and PGR.
Aberrant DNA methylation of the T-DMR was associated with the impaired expression of ESR1, but not the altered expressions of ESR2 and PGR, in OE.
KeywordsEndometriosis Ovarian endometrioma Eutopic endometrium Steroid receptor Estrogen receptor 1 Estrogen receptor 2 Progesterone receptor DNA methylation
Estrogen receptor alpha
Estrogen receptor beta
Estrogen receptor 1
Estrogen receptor 2
Quantitative real-time RT-PCR
Tissue-dependent and differentially methylated region
Endometriosis is a common gynecological disease affecting approximately 10% of reproductive age females , and is characterized by the ectopic localization of endometrial-like tissue in the pelvic cavity . The disease induces chronic inflammation in the pelvic cavity, leading to symptoms such as chronic pelvic pain and infertility that subsequently affect the patient’s health [1, 2] .
Endometriosis develops mostly in women of reproductive age and regresses after menopause, suggesting that the growth is estrogen-dependent. Estrogen hormone action is mediated by the estrogen receptor in many physiological and pathological processes. The estrogen receptor has two subtypes, estrogen receptors α and β (ERα and ERβ) encoded by estrogen receptor 1 (ESR1) and 2 (ESR2), respectively [3, 4]. ESR1 is the primary mediator of the estrogenic action, and its expression of ESR1 is higher than that of ESR2 in endometrium . In endometriotic tissue, the expression of ESR1 is strongly suppressed, while ESR2 is upregulated . However, the exact mechanisms of the downregulation of ESR1 and upregulation of ESR2 in endometriotic tissue remain unclear [7, 8].
DNA methylation is a well-known epigenetic mark. DNA methylation, which occurs at CpG sites, interrupts the recognition and binding of transcription factors , recruits methyl CpG-binding proteins that interact with transcription repressors , and induces chromatin condensation via histone modification changes . Maintaining a specific DNA methylation profile in a cell is necessary for cellular integrity, and alterations in DNA methylation may have serious health consequences . We and other groups have previously demonstrated that aberrant epigenetic regulation is associated with the pathogenesis and development of endometriosis [10, 11, 12, 13] . In fact, local estrogen production is upregulated in endometriotic tissue by altering the activities of enzymes involved in synthesizing or degrading estradiol [10, 14, 15]. We showed that the altered activities of these genes were caused by aberrant DNA methylation [10, 16].
The tissue- and cell-specific expression of a gene can be determined by DNA methylation of a specific region of the gene called the tissue-dependent and differentially methylated region (T-DMR) . Recent genome-wide analyses have identified many T-DMRs in mammalian genomes [17, 18, 19]. T-DMRs tend to be located at an upstream region of gene promoter or intron rather than in the gene promoter region [18, 19, 20, 21]. We previously reported that two T-DMRs are present in the region from − 0.5 kb to − 1 kb with respect to the transcription start site of ESR1, and DNA methylation of the two T-DMRs, but not the promoter region, regulate tissue-specific expression of ESR1 in normal tissues . In the tissues with DNA hypermethylation in the two T-DMRs, the expression of ESR1 is suppressed . This led us to hypothesize that the downregulation of ESR1 in endometriosis is caused by aberrant DNA methylation of these T-DMRs. On the other hand, DNA methylation of the promoter region (not the T-DMRs) was reported to regulate the expression of ESR2 . However, because the T-DMRs have a role in ESR1 expression, there is a possibility that regions other than the promoter region are involved in the regulation of ESR2 expression.
Resistance to progesterone may contribute to the pathophysiology of endometriosis , and may be caused by aberrant expression of progesterone receptor (PGR) . In cancer tissues, PGR expression may be regulated by DNA methylation of the proximal promoter region of PGR [25, 26]. However, it remains unclear whether aberrant DNA methylation is also involved in the suppressed expression of PGR in endometriosis.
In the present study, we first compared the expressions of ESR1, ESR2 and PGR in eutopic endometrium (EE) and ovarian endometrioma (OE). Next, we examined the DNA methylation statuses of ESR1, ESR2 and PGR to investigate whether aberrant DNA methylation is associated with their aberrant expressions in OE.
mRNA expressions of ESR1, ESR2 and PGR
ESR2 has two isoforms encoded by ESR2 variants a and b (Fig. 1b). The expression levels of the two variants were examined together using a primer pair that detects both variants. The expression of ESR2 was 134-fold higher in OE than in EE (Fig. 2b).
PGR has two isoforms, PR-A and PR-B, encoded by PGR variants 1 and 2, respectively (Fig. 1c). Since the primer pair designed for PGR variant 1 also detects PGR variant 2, the amplified product reflects the combined levels of PGR variants 1 and 2. The combined expression level of PGR variants 1 and 2 was 12-fold lower in OE than in EE (Fig. 2c). The expression of PGR variant 2 was 8-fold lower in OE than in EE (Fig. 2c).
DNA methylation statuses detected by 450K BeadChip
DNA methylation statuses detected by sodium bisulfite sequencing
We previously reported that DNA methylation of the T-DMRs of ESR1 regulates the expression of ESR1 in normal tissues . The expression of ESR1 in the tissues with high DNA methylation at the T-DMRs was strongly suppressed compared with the expression of ESR1 in the tissues with low DNA methylation at the T-DMRs . In the present study, ESR1 expression was lower and DNA methylation at the T-DMRs of ESR1 was higher in OE than in EE, indicating that the aberrant DNA methylation of the T-DMRs was associated with the impaired expression of ESR1 in OE. On the other hand, DNA methylation was not involved in the altered expressions of ESR2 and PGR in OE.
ESR1 is downregulated in cancer tissues such as breast cancers, and the downregulation was shown to be associated with DNA methylation of the promoter region of ESR1 [28, 29, 30]. However, Meyer et al.  found no significant difference in the DNA methylation status of ESR1 at the promoter region between EE and OE. We previously showed that methylation of the promoter region (not the T-DMR) of ESR1 was low in normal tissues with both high and low ESR1 expression [20, 31]. These results indicated that the DNA methylation of the T-DMRs regulates the expression of ESR1 and there is no difference in DNA methylation of the promoter regions between tissues with different expression levels of ESR1 . The present results show that the methylation levels of the promoter regions were kept low in both OE and EE, which indicates that the impaired expression of ESR1 in OE is due to the T-DMRs and not the promoter region.
ESR2, which is upregulated in OE, has been shown to suppress ESR1 expression in endometriosis by binding to the ESR1 promoter [8, 32] . Knockdown of ESR2 in endometriotic stromal cells increased the expression of ESR1 twofold, and overexpression of ESR2 in endometrial stromal cells reduced the expression of ESR1 by one-half [8, 32]. In view of the huge difference in the expression levels of ESR1 between EE and OE (Fig. 2a), aberrant DNA methylation of the T-DMRs is likely a factor in the impaired expression of ESR1 in OE.
Xue et al. reported that the DNA methylation levels of the promoter region of ESR2 is high in endometrial stroma, while it is low in endometriotic stroma . They concluded that the increased expression of ESR2 in endometriosis is caused by DNA hypomethylation of the promoter region (from − 163 to − 48) of ESR2 [22, 32]. However, 25% of their endometrial stroma samples also showed DNA hypomethylation. Because of this inconsistency, they may have shown only a difference among individuals. The present results show that DNA methylation in the region from − 163 to − 48 was low in both EE and OE. We also did not see any difference in DNA methylation in the regions upstream and downstream of this region; in these regions, both EE and OE were DNA hypomethylated. Our results suggest that DNA methylation is not involved in the upregulation of ESR2 in OE.
The expressions of PGR variants 1 and 2 were significantly different between EE and OE. The present study showed no significant difference in the DNA methylation status between EE and OE, indicating that DNA methylation is not associated with the differential expression of PGR. However, in breast cancer tissues, DNA methylation around the transcription start sites appears to have a role in the regulation of PGR expression [25, 26]. Aberrant DNA methylation often occurs in the promoter region in a variety of cancers [33, 34]. In fact, we previously showed that DNA hypermethylation of the ESR1 promoter occurred only in cancer tissues and that this region remained hypomethylated in non-cancer tissues regardless of the ESR1 expression level . Therefore, the difference between the previous reports and the present study may be due to a difference in the examined samples. Other mechanisms such as the binding of transcription factors may be associated with the differential expression of PGR between EE and OE.
We found that OE had aberrant DNA methylation in the T-DMRs of ESR1 and that the DNA methylation is associated with the impaired expression of ESR1 in OE. On the other hand, it is unlikely that DNA methylation is associated with the altered expressions of ESR2 and PGR in OE.
Specimens of EE and OE were obtained from 16 Japanese women. EE was obtained from patients who underwent surgery for uterine leiomyoma. The ages of the patients were 32.13 +/− 4.19 years old (mean +/− SD; 26–40 years). OE tissue was obtained from patients who underwent cystectomy of OE during the proliferative phase [10, 16]. The ages of the patients were 31.13 +/− 5.08 years old (mean +/− SD; 24–39 years old) and did not significantly differ from the patients with uterine leiomyoma (p = 0.67). None of the patients had received previous hormonal therapy. Specimens were dissected immediately after removal, immersed in liquid nitrogen and stored at − 80 °C until DNA/RNA extraction as previously reported [35, 36].
Quantitative real-time RT-PCR (qRT-PCR)
Primer sets used in quantitative RT-PCR
variant1 (transcribed from uExon-A)
variant2 (transcribed from uExon-B)
variant3 (transcribed from uExon-C)
F: 5′ -CTCGCTTTCCTCAACAGGTG- 3′
F: 5′ -ACCAGCTCTTGGTGCCTGT- 3′
F: 5′ -TCCCTCTGCCCCTATATTCC- 3′
Illumina Infinium HumanMethylation450k BeadChip assay
Genomic DNA was isolated from 20 mg of frozen tissue using a Qiagen Genomic DNA kit (Qiagen). DNA methylation was analyzed with an Illumina Infinium assay with the HumanMethylation450 BeadChip (Illumina, San Diego, CA, USA), which interrogates a total of 482,421 CpG sites from the distal promoter regions of the transcription start sites to the 3’-UTR of consensus coding sequences. Methylated and unmethylated signals were used to compute beta values, which are quantitative scores of the DNA methylation levels, ranging from 0 (completely unmethylated) to 1 (completely methylated). The BeadChip was scanned on a BeadArray Reader (Illumina) according to the manufacturer’s instructions. CpG sites with “detection p values” > 0.05 (computed from the background based on negative controls) and CpG sites on the Y chromosome were eliminated from further analysis, leaving 482,005 CpGs valid for use with the nine samples tested.
Sodium bisulfite sequencing
Primer sets used in sodium bisulfite sequencing analysis
ESR1_Region I (− 129 to + 279)
ESR1_Region II (− 670 to −94)
ESR1_Region III (− 1298 to − 731)
ESR1_Region IV (− 2227 to − 1756)
ESR1_Region V (− 2595 to − 2241)
ESR1_Region VI (− 2981 to − 2573)
ESR2 (− 113 to + 3)
DNA methylation and mRNA Expression levels of the two groups were compared with unpaired t-tests using SPSS for Windows version 11 (SPSS Inc., Chicago, IL, USA).
This work was supported in part by JSPS KAKENHI Grants C16K11142, C16K11091, C18K09262, C17K11239, C18K09230, 18 K16801 and 18 K16772 for Scientific Research from the Ministry of Education, Science, and Culture, Japan.
Availability of data and materials
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
RM is the first/corresponding author, and participated in the study designing, supervision, experiment, analysis of BeadChip, statistical analysis and interpretation of the data and drafting of the manuscript. YM, SS, MO, IT, MS, YS, HT and TT participated in the measurement of the levels of mRNA expression and DNA methylation, interpretation of the measured data, and drafting the manuscript. IT and MS participated in the analysis of BeadChip and statistical analysis. HT and NS conceived, designed and supervised the study and participated in drafting the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
This study was reviewed and approved by the Institutional Review Board of Yamaguchi University Graduate School of Medicine. Written informed consent was obtained from the participants before the collection of any samples, and the specimens were irreversibly de-identified. All experiments involving the handling of human tissues were performed in accordance with Tenets of the Declaration of Helsinki.
Consent for publication
Written informed consent for publication was obtained from the participants before the collection of any samples.
The authors declare that they have no competing interests.
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