Advertisement

Malaria Journal

, 17:346 | Cite as

On the epidemiology of Plasmodium vivax malaria: past and present with special reference to the former USSR

  • Anatoly V. Kondrashin
  • Lola F. Morozova
  • Ekaterina V. Stepanova
  • Natalia A. Turbabina
  • Maria S. Maksimova
  • Evgeny N. Morozov
Open Access
Review

Abstract

Presently, many malaria-endemic countries in the world are transitioning towards malaria elimination. Out of the 105 countries with ongoing malaria transmission, 10 countries are classified as being in the pre-elimination phase of malaria control, and 9 countries are in the malaria elimination stage, whereas 7 countries are classified as being in the prevention of introduction phase. Between 2000 and 2015, 17 countries eliminated malaria (i.e., attained zero indigenous cases for 3 years or more). Seven countries were certified by the WHO as having successfully eliminated malaria. The purpose of this review was to analyse the epidemiological characteristics of vivax malaria during the various stages of malaria eradication (elimination) programmes in different countries in the past and present. Experiences of the republics of the former USSR with malaria are interesting, particularly since the data overwhelmingly were published in Russian and might not be known to western readers. Among the most important characteristics of Plasmodium vivax epidemiology at present are changes in the ratio of the short-incubation P. vivax to long-incubation P. vivax, the incidence of severe P. vivax cases, the increased numbers of asymptomatic P. vivax cases, the reduced response to anti-malarials and a few others. Various factors contributing towards the peculiarities of P. vivax epidemiology are discussed.

Keywords

Plasmodium vivax Elimination Epidemiology Severe malaria Asymptomatic malaria Primaquine G6PD deficiency Long incubation 

Abbreviations

PCD

passive case detection

PCR

polymerase chain reaction

USSR

Union of Soviet Socialist Republics

WHO

World Health Organization

Background

Many malaria-endemic countries are transitioning towards malaria elimination. At present, out of 105 countries with ongoing malaria transmission, 10 countries are classified as being in the pre-elimination phase of malaria control, and 9 countries are in malaria elimination stage, whereas 7 countries are classified as being in the prevention of introduction phase. Between 2000 and 2015, 17 countries eliminated malaria (i.e., attained zero indigenous cases for 3 years or more) [1].

Globally, more countries are moving towards elimination; in 2016, 44 countries reported fewer than 10,000 malaria cases, up from 37 countries in 2010. Kyrgyzstan and Sri Lanka were certified by WHO as malaria-free in 2016, thus contributing to the total of 7 countries certified during the last few years. In 2016, WHO identified 21 countries with the potential to eliminate malaria by the year 2020 [2]. Experiences in malaria eradication (in the past) and elimination (at present) have revealed that in the countries with local transmission of both falciparum and vivax malaria, the successful implementation of various anti-malaria measures results first in the disappearance of Plasmodium falciparum, followed by its elimination. This phenomenon is due to the biological characteristics of Plasmodium vivax, particularly the presence of the hypnozoites responsible for relapses of this species. In addition, various genotypes of P. vivax demonstrate differences in the primary appearances of clinical signs of the disease, thus dividing the parasite populations into 2 groups: P. vivax with a short or long incubation period. On average, as shown from experience, the elimination of P. vivax foci can be achieved, but not in fewer than 3 years, compared with the elimination of P. falciparum, which can be achieved in 1 year [3].

The purpose of this review is to analyse the epidemiological characteristics of P. vivax during the various stages of malaria eradication (elimination) programmes in different countries in the past and present. The experiences of republics of the former USSR with malaria are interesting, particularly because an overwhelming amount of data was published in Russian and might not be known to western readers.

Among the most important characteristics of P. vivax epidemiology at present are changes in the ratio of P. vivax with short incubation to P. vivax with long incubation, an increased incidence of severe P. vivax cases, increased numbers of asymptomatic P. vivax cases, reduced response to anti-malarials and a few others.

Changed short-to-long incubation ratio in vivax malaria

Populations of P. vivax with long incubation were originally confined to areas with a temperate climate, such as northern, eastern and central Europe, northern parts of Asia and America, whereas P. vivax with short incubation has overwhelmingly been distributed in areas with sub-tropical and tropical climate. However, during the last few decades, a well-established trend of proliferation of P. vivax with long incubation is occurring to the south. Such events could have implications.

For example, prior to the launch of large-scale malaria control activities in the area of the European part of the former USSR, P. vivax with long incubation constituted approximately 70–80% of the total malaria cases. Primary clinical manifestations usually occurred at 8- to 14-month intervals following contraction of infection. However, in the southern parts of the country, the proportion of vivax malaria with long incubation accounted for approximately 10% of the total malaria cases, with the remaining cases being vivax malaria with short incubation [4] (Fig. 1).
Fig. 1

Ratio of Plasmodium vivax short incubation to long incubation

With the implementation of countrywide activities of the national malaria eradication campaign during the 1950s and 1960s, the intensity of malaria transmission was considerably reduced, which resulted in the alteration of the ratio exemplified by the malaria situation in the Republic of Azerbaijan.

During the 1940s and 1950s, P. vivax with short incubation was the predominant species in the Republic of Azerbaijan, accounting for up to 90% of the total malaria cases. No cases of P. falciparum were registered at all. By the end of the 1960s, the territory of the Republic was free of malaria except for the continued transmission in a few residual foci of vivax malaria.

Due to the interaction of various factors, a large-scale epidemic of vivax malaria occurred in the plains of the Republic of Azerbaijan in the 1970s. During the course of the epidemic, the number of primary clinical manifestations on the eve of the start of local malaria transmission (month of May) dramatically increased, suggesting the occurrence of malaria with long incubation. Such cases constituted 32–36% of the total number of cases detected during the years 1971–1973 [5] (Fig. 2).
Fig. 2

Ratio of Plasmodium vivax with short incubation to that with long incubation (Republic of Azerbaijan 1980s and 1990s)

Results of studies in 3 P. vivax residual malaria foci in the Geok-Chai District of Azerbaijan in 1980–1983 revealed that the fraction of P. vivax with long incubation constituted 49% of the cases, and some cases were detected up to 28 months after contraction of infection [6].

In the Republic of Tajikistan, P. vivax cases with short incubation constituted more than 95% of the total cases during the 1940s and 1950s (Fig. 3).
Fig. 3

Ratio of Plasmodium vivax short incubation to long incubation (Republic of Tajikistan 1990 and 2000s)

However, in 1988–1990, in the Punj district bordering Afghanistan, 45% of all detected cases were classified as P. vivax cases with long incubation [7]. In other border areas with Afghanistan, the proportion of cases with long incubation constituted 55% [8]. Among the imported cases of P. vivax in Soviet servicemen from Northern Afghanistan during the 1980s, 54% were P. vivax cases with long incubation. The primary clinical manifestations of these cases were registered from 6 to 38 months after contraction of infection [9].

Reports from other parts of the world, particularly from the Indian continent and Southeast Asia have further confirmed that, at present, P. vivax with long incubation is no longer a legacy of only those countries with temperate climate [10, 11]. Now, P. vivax phenotypes with long incubation are believed to be more widespread and more prevalent than previously thought [12]. For example, in China, where P. vivax is a predominant malaria species, the ratio of short to long incubation is approximately 1:1 [13, 14].

As in the past, North Korea continues to be the stronghold of P. vivax strains, with more than 70% of the malaria cases being P. vivax with long incubation [15].

In India, studies in the western territories (Gujarat State) revealed the presence of P. vivax malaria with long incubation [16]. The presence of local strains of vivax malaria with long incubation in Delhi (north/central India) was confirmed during studies in 1988–1993 [10]. The existence of P. vivax with long incubation in Central India was reconfirmed by the results of studies carried out in Aligarh [17]. The results of genotyping of P. vivax carried out in the eastern part of the country (Kolkata) revealed the presence of locally transmitted infection with primarily long incubation, which occurred 81% of the time compared with short incubation at 19% [11].

The results of these observations have important bearings on the organization and implementation of anti-malarial activities. Unlike the case of P. falciparum, elimination of vivax malaria foci cannot be achieved during a 1-year period, thus necessitating the continuation of interventions and observations for at least 3 consecutive years and also increasing the cost of the operations. Another important peculiarity of the epidemiology of P. vivax at present is the increase in the number of reported cases of severe infection.

Incidence of severe Plasmodium vivax cases in the former USSR

In the former USSR, prior to malaria elimination in the country, malaria was one of the major health problems (see Additional file 1), and complicated cases of P. vivax were registered quite regularly [18]. During the 1930s and 1940s, an epidemic of so-called P. vivax ‘fulminant malaria’ known for causing death, occurred on the territory of the European part of the USSR and in Kyrgyzstan and Uzbekistan (Fig. 4). Fulminant malaria was common among both children and adolescents and rather rare among adults. Normally, cases of fulminant malaria were reported during the spring and summer months, coinciding with primary manifestations of the disease or following 2–3 relapses in the case of P. vivax with long incubation. Death could occur within 2–3 h [18] (see Additional File 2). Russian clinicians believed that such forms of vivax malaria were not only species-specific but were also related to the health state of the infected individual in conjunction with a very poor socio-economic environment [19].
Fig. 4

Registered Plasmodium vivax fulminant cases in the former USSR

Detailed descriptions of severe cases of vivax malaria in the USSR have been presented in monographs and various journals in Russian. The opinion of Soviet clinicians was that the major cause of severe disease was due to ‘secondary hypochrome’ anaemia from prolonged high parasitaemia [20]. Modern explanations of the phenomenon agree with this statement. High parasitaemia in P. falciparum (approximately 100,000 parasites/µl) is less pernicious than parasitaemia of 10,000–20,000 parasites/µl in P. vivax, as the latter persists in the organism considerably longer than in cases of P. falciparum [21].

Cases of comatose P. vivax were reported in approximately 5% of the total comatose malaria cases and exclusively during malaria epidemics [18]. Rupture of the spleen was one of the most serious complications in P. vivax, leading to acute abdominal pain accompanied by a sharp reduction in blood pressure, internal haemorrhage and shock [8, 20]. Other manifestations (1–2% of total complications) were haemoglobinuria, acute nephritis, hypertension, haematuria, albuminuria, and several other rare syndromes [18].

Present increase of severe vivax malaria in the world

An increase in the numbers of severe cases and even cases of deaths due to P. vivax in the world marked the beginning of the 21st Century and coincided with the commencement of national programmes of malaria elimination. This increase resulted in the appearance of a number of publications on the subject in different parts of the world [22, 23, 24, 25].

As could be expected, more than 40% of all publications on P. vivax come from India, the country with the world’s largest national malaria control programme and where the annual number of P. vivax cases constitutes 47% of all malaria cases [26]. Serial cases of severe P. vivax have been reported in the USA, Indonesia and Pakistan. Sporadic cases have also been reported in Laos, Cambodia, Thailand, China, DPR Korea, Bangladesh, Afghanistan, countries of the Middle East and the African Horn, Madagascar, Brazil, and Papua New Guinea [27].

Among the major clinical signs of complicated vivax malaria, severe thrombocytopaenia (< 50,000 cub.mm) is the most common manifestation. The association of severe thrombocytopaenia with acute anaemia can result in death of the malaria patient [28]. Acute anaemia in vivax malaria constitutes a high mortality risk for young children and pregnant women, with a reported case fatality rate of 0.3% [22, 25].

Overall, severe illness with acute infection includes lung injury with respiratory distress, kidney injury with renal dysfunction, hepatic dysfunction and jaundice, seizures/delirium/coma, severe thrombocytopaenia or circulatory collapse [29]. The severity of the syndromes varied widely among the different geographical areas, with severe anaemia being most prominent in areas of high transmission, frequent relapses and chloroquine resistance [24, 25].

Sporadic cases of complicated vivax malaria described in India and Brazil revealed a few other syndromes such as jaundice, haemoglobinuria, seizures, and pulmonary oedema. A combination of syndromes such as jaundice, thrombocytopaenia, anaemia, renal dysfunction, respiratory distress, and cerebral malaria resulted in the death of patients in India [30].

Plasmodium vivax-related anaemia is a high risk for children. Thus, in malaria-endemic territories of Indonesia, approximately 25% of the total inpatients with apparent anaemia are young children [31]. Severe P. vivax-related anaemia among hospitalized children in Papua (Indonesia) was the cause of death at the level of 10.3 per thousand cases [22].

The details of P. vivax-related anaemia in pregnant women were determined in Russia. The most frequent complications were premature delivery, abortions, prolonged course of the disease, and specially increased frequency of relapses towards the end of pregnancy and immediately after delivery [18]. The risk of severe anaemia in pregnant women affected by P. vivax is double that of non-pregnant women with vivax malaria [32]. Studies undertaken in Peru in areas with relatively low levels of vivax malaria transmission revealed that even a single attack of infection during the first trimester of pregnancy increased the risk of spontaneous abortion by four times [33].

The situation has further been aggravated by the exclusion of pregnant women and young infants/children from the use of a gametocytocidal drug (primaquine) in P. vivax infection, thus contributing to local malaria transmission being an important source of infection [21].

Severe P. vivax cases can be reported in situations with very low local transmission. Two cases of severe vivax malaria were described in such a setting in the Russian Federation. One was an introduced case in Volgograd city in 1998 in a 62-year-old man. Malaria was laboratory-confirmed only on the 10th day after primary clinical manifestations. Detailed clinical examination of the patient revealed the presence of severe anaemia and liver cirrhosis. In spite of the anti-malaria treatment undertaken, the patient died [34]. Another introduced case occurred in Moscow in 2001 in a man 35 years of age. The patient was admitted to the hospital with acute spleen pain and haemorrhage which necessitated a splenectomy due to rupture; subsequently, parasites of P. vivax were detected in the patient’s blood [34].

Clinical and parasitological manifestations of Plasmodium vivax in the elimination stage

The intensity of reproduction of the malaria parasite determines both the clinical course of the infection and the stability of the epidemiological process. Evidence exists of differences in clinical and parasitological manifestations during the increase/decrease of malaria incidence, corresponding to various periods of malaria season [35].

Duchanina [36] described a mild clinical course of P. vivax infection with long incubation during the final stages of malaria eradication in the former USSR in comparison with progression in the pre-eradication period. This phenomenon was attributed to contraction of malaria from a mosquito infected by a small number of sporozoites and the absence of repeated infection by mosquito [36].

A similar situation was found in Tajikistan and in Azerbaijan in the course of the final stage of containment of a malaria epidemic during the 1980s. A marked decrease occurred in the clinical manifestations, along with low parasitaemia among cases with long incubation, which was accompanied by an increase in the number of asymptomatic carriers [37, 38]. A mild course of P. vivax infection was observed among 92 children under 14 years of age in one study in the Punj district of southern Tadjikistan in 1988–1990. Infected children with body temperatures of 37.2–38.0 °C did not have any complaints and attended school classes [39]. Thermometry surveys among women engaged in the cotton harvesting in Azerbaijan detected appreciable numbers of malaria patients without complaints while their body temperature was 38 °C (Kondrashin, pers. comm.).

In Azerbaijan, laboratory examination of blood slides taken from P. vivax patients with long incubation in the residual foci demonstrated lower parasite densities in comparison with the density of parasites in the blood films taken from patients with short incubation [40]. Data from the National Malaria Reference Centre in Baku, Azerbaijan, showed a decrease in parasite densities of P. vivax was associated with a decrease in the intensity of malaria transmission. In 1974–1976, at the height of malaria outbreak, the proportion of positive blood slides for P. vivax with a low density of parasites was approximately 20%, while this proportion was 37% at the beginning of the 1980s following a reduction in malaria transmission intensity [41].

An implication of low clinical manifestations and parasitaemia in malaria cases following a reduction in the intensity of malaria transmission is difficult in the case of detection; low clinical manifestation prolongs the time for laboratory confirmation of malaria diagnosis and necessitates the deployment of much more sensitive and costlier diagnostic methods such as PCR, etc. Reduced clinical manifestation also suggests that long-incubation P. vivax is more prone to survive in the form of asymptomatic case.

Factors facilitating incidences of severe vivax malaria

Unification of the definition of ‘severe P. vivax case’ recently by the WHO has facilitated the reporting of such cases by the personnel of health treatment facilities [42]. This was done through the modification of the definition of ‘severe P. falciparum case’. Severe vivax malaria is defined as it is for falciparum malaria but with no parasite density threshold [42]. For example, the results of observations in one hospital in India revealed that, among the vivax malaria patients, 15% were reported cases of complicated disease [23].

Comparative epidemiological role of Plasmodium vivax with short and long incubation

The results of genotyping of P. vivax parasites (presumably relapses) carried out in the vicinity of Kolkata (India) revealed active transmission of both short- and long-incubation phenotypes [11]. Furthermore, the proportion of true relapses was 60%, whereas that of re-infection constituted 40%. Detailed analysis revealed that relapses due to short incubation were genetically homogenous in 69% of the cases, whereas the remaining relapses were genetically heterogeneous [11]. Implications of these findings are that re-infection due to heterogeneous strains, as a rule, result in a more severe disease course [20]. The same phenomenon is observed in a case of re-infection by a malaria parasite other than P. vivax, particularly re-infection by P. falciparum [20].

One of the peculiarities of the epidemiology of vivax malaria with long incubation in the former USSR was the modulation of the number of relapses depending on the transmission level of infection (Fig. 5).
Fig. 5

Incidence of relapses of vivax malaria with long incubation in foci with various level of transmission [43]

The frequency of relapses depending on the transmission level has an important bearing on the clinical course of vivax malaria. Strains with short intervals between relapses, as in the case of the Chesson strain and strains with long incubation, have negative effects on the haematological state of an infected individual in comparison with P. vivax strains with a long interval between relapses. The cause of such a phenomenon is that the appearance of short relapses overrides the ability of the blood system to return to its normal function following previous attacks of the disease. Such a relapse results in an absolute reduction in the red blood cell mass due to the greater removal of uninfected red blood cells [32] (Fig. 6).
Fig. 6

Malaria in Russia and the former USSR (1900–1963)

The main difference between P. vivax primary manifestations and relapse is in the abrupt appearance of malaria syndromes in the case of the latter. At the same time, the level of parasitaemia is considerably higher compared with primary clinical manifestations. The level of parasitaemia could reach 5000 and even 10,000 parasites/ml compared with 200–500 parasites. However, the patient feels much better than during the primary manifestations due to the development of some level of immunity. Importantly, along with the increased density of parasites, a parallel augmentation occurs in the density of gametocytes. The results of one study in Georgia (in the former USSR) during the 1950s and1960s revealed that the density of gametocytes during P. vivax relapse was two times higher than during the primary manifestations of the disease [44]. Thus, the epidemiological role of P. vivax relapses could be seen in the enhancement of malaria transmission due to the higher density of gametocytes on the one hand and the potential increase of asymptomatic malaria on the other hand.

Role of relapse pattern in the epidemiology of vivax malaria

Various strains of P. vivax differ in their innate ability to relapse. Thus, the ‘Madagascar’ strain was known for its ability to relapse in approximately 80% of cases in comparison with the ‘Holland’ strain, which relapsed in only 10% of the cases [3]. The relapse rate in the ‘Chesson’-like strains distributed in the area of the Western Pacific and Southeast Asia is even higher (more than 80%) [45]. On the other hand, P. vivax in the area of the sub-Indian continent (India, Nepal, Sri Lanka) historically is known for its relatively low ability to produce relapse [46]. In India, during the 1960s, the maximum level of relapses in local P. vivax was approximately 40% [47]. The dynamics of the innate ability of Indian strains of P. vivax to relapse are presented in Table 1.
Table 1

Dynamics of the innate ability to produce relapse and its frequency after 5-day treatment with primaquine in India (1960–2012)

Years

Source

Innate ability %

Relapse rate after 5-day treatment with primaquine

1960

Basavaraj [47]

40.00

5.75

1990

Sharma et al. [48]

40.00

2.60

1996

Srivastava et al. [16]

16.00–27.00

2.50–5.80

1998

Gogtay et al. [49]

8.30

3.00

1998

Adak et al. [10]

23.00–44.00

NA

2001 (a)*

Adak et al. [50]

40.00

27.00–30.00

2001 (b)*

Adak et al. [50]

21.80

19.80

2002

Yadav et al. [51]

8.60

5.70

2012

Kim et al. [11]

31.00

27.00

*Two different sites

During the appraised period, the innate rate of relapse hardly exceeded 40%, and in some areas, it was even much lower. The efficacy of a 5-day treatment with primaquine, particularly in conjunction with the use of insecticides on a large scale, was quite efficacious both from an operational and epidemiological point of view. However, the situation began to change the beginning of the 21st Century, when the efficacy of a 5-day treatment practically reached zero [51]. In spite of evident failing efficacy of a 5-day treatment with primaquine, the use of primaquine continued to be the official method of anti-relapse treatment recommended by the national malaria control programme in India until 2007. The replacement of the use of the 5-day treatment with the standard 14-day treatment took place only in 2008. The introduction on a national scale of the 14-day treatment was accompanied by several operational problems, with the very low level of compliance by the affected population being a significant problem [52]. The result was thousands of improperly treated people, who potentially were contributing to (a) the enhancement of local P. vivax transmission; (b) an increased number of severe cases; and, (c) an increased number of asymptomatic cases [52].

The malariological situation likely was further aggravated by the replacement of local strains of P. vivax with a low ability to relapse with strains with a high ability that was facilitated by large-scale, uncontrolled population movement from different malaria-endemic countries, including Papua New Guinea, Indonesia, Myanmar, Thailand, Afghanistan, and Pakistan. A probable mechanism of strain replacement might be the recombination of the various genotypes of the parasite, contributing to the biological diversity of the species [12].

Role of asymptomatic infection in the malaria elimination process

Malaria infection in the form of asymptomatic parasitaemia occurs quite frequently in the world. In the former USSR, prior to the launch of a national malaria eradication programme, the prevalence of asymptomatic parasite carriers constituted 10–18% of all detected malaria cases during mass surveys of the population, particularly on the eve of the beginning of malaria transmission season in May and June [53].

In the sub-tropical Massali district of Azerbaijan during the final stage of malaria eradication, mass blood surveys in 2 active malaria foci in 1960 revealed that the prevalence of asymptomatic P. vivax cases was 23.7% of the total number of cases in one village and 56.2% in another village. The majority of detected carriers were children under 14 years of age [54].

In tropical areas, the frequency of asymptomatic P. vivax varies from 30 to 50% among the children and to a somewhat lesser extent among adults. Thus, a large proportion of asymptomatic infections of both P. falciparum and P. vivax, with low and sub-microscopic parasite densities, was found in the low-transmission setting of Temotu Province, Solomon Islands, in 2008 [55]. Only 17.8% of P. falciparum- and 2.9% P. vivax-infected subjects were febrile at the time of the survey. A significant proportion of both infections detected by microscopy showed a parasite density below 100 parasites/µl. An age correlation accounted for the proportion of low parasite density for P. vivax only [55].

In the Bandarban District of Chittagong Hill Tracts of southeastern Bangladesh, a large proportion of asymptomatic P. falciparum and P. vivax infections were found by microscopic and PCR examination. The proportion of asymptomatic infections among the confirmed PCR cases was 77%. Significantly more asymptomatic cases were recorded among the patients older than 15 years of age, whereas prevalence and parasite density were significantly higher in patients younger than 15 years of age [56]. A similar pattern was noted among the imported cases from Africa and Asia to the Russian Federation [57].

The possibility of transmission of malaria infection by Anopheles mosquitoes feeding on an infected human with a very low density of parasites in the peripheral blood was experimentally demonstrated in Russia as early as the end of the 1930s and beginning in the 1940s [58, 59]. At present, individuals with very low parasitaemia and/or with asymptomatic malaria provide an important reservoir for malaria transmission and smear-positive asymptomatic cases are more infectious to mosquitoes than those with sub-microscopic infection [60].

The genesis of asymptomatic malaria is largely of an immunological nature. The intensity of parasitaemia progressively decreases due to the active formation of anti-parasite complex immunity, whereas pyrogenic thresholds prevent malaria patients from undergoing malaria paroxysms under conditions of reduced density of malaria parasites. In other words, asymptomatic malaria disease is a consequence of infection experienced sometime in the past. The phenomenon of asymptomatic malaria is a potential threat to the infected person and to the community as well [57].

Asymptomatic malaria in an infected person is a good example of a well-balanced status between the host and parasite. However, this balance can change in favour of the latter due to various reasons (re-infection, environmental changes, reduction of immunity, etc.). Such disruption of the balance can occur at any time as the longevity of asymptomatic infection varies quite widely, and the density might be very low. In Plasmodium malariae, for example, asymptomatic infection can last for many years. Epidemiologically, the patient with malaria could possibly lead to the patient with asymptomatic malaria infecting a recipient (transfusion malaria). In the case of reduced immunity in the patient with asymptomatic malaria, the disease might return in a quite severe clinical form [57].

As far as the communities are concerned, asymptomatic malaria cases that harbour infective gametocytes are quite efficient in the maintenance of malaria transmission and are largely responsible for the persistence of malaria foci [61].

Various factors and conditions might facilitate the development of asymptomatic malaria. A broad range of factors associated with the development of asymptomatic malaria was recently reviewed [61]. Among the factors listed were infection prevalence, mosquito infectivity, parasite density, strain diversity, and many others. The role of factors such as polymorphism of immunologically relevant genes and genetic disorders (G6PD deficiency, β-thalassaemia, etc.), malaria infections in association with different parasitic diseases and infections, and others was also mentioned [61].

Role of G6PD deficiency in asymptomatic vivax malaria

G6PD deficiency is a common genetic disorder in humans. This deficiency is prevalent throughout Africa, Asia, Southeast Asia and parts of South America, where malaria is endemic [62]. At present, the prevailing hypothesis is that G6PD deficiency confers protection from severe malaria disease caused by P. falciparum. The basis of this hypothesis was the results of several population-based studies indicating more light clinical manifestations of the disease among individuals with the G6PD deficiency accompanied by low parasitaemia [63, 64, 65, 66]. Thus, a mild course of P. falciparum infection potentially results in asymptomatic malaria.

The relevance of this hypothesis to P. vivax was tested in Azerbaijan during the epidemic in 1970 to 1973. Epidemiological studies undertaken in the Republic found the G6PD deficiency present among local population at various levels (from 2.8% to 38.7%, with an average of 10.0%) [67]. This deficiency was highest among the people of the Geok-Chai District (on average 15.4%). The aim of studies undertaken in the Geok-Chai District was to establish whether the carriers of the deficient gene had a protective advantage against contracting vivax malaria. The studies were carried out in a group of villages with a total population of approximately 8000 people and with a malaria prevalence of 5.94%. Prevalence of the G6PD was studied in 125 vivax malaria cases confirmed by laboratory and among 604 persons determined as negative by laboratory testing. Positive cases consisted of patients with clinical manifestations of malaria and asymptomatic cases. The results of the investigations are presented in Table 2.
Table 2

Prevalence of G6PD among malaria patients and healthy persons, Azerbaijan, 1972 [67]

Groups

Total examined

G6PD deficient

%

OR

CI

p- value

+ve

125

11a

8.80

0.36

0.18–0.72

0.001

−ve

604

127

21.03

a 7 asymptomatic cases

The G6PD prevalence among malaria patients was lower than in non-infected persons (p < 0.05). Although statistically significant, the OR of 0.36 indicates that the G6PD deficiency does not prevent the contraction of infection. Even more epidemiologically important, however, is that among the 11 G6PD-deficient positive cases, 7 persons were asymptomatic cases, and only 4 positive cases were with clinical manifestations of the disease, suggesting that the G6PD deficiency played some role in the phenomenon of asymptomatic infection [67].

The results of the studies in Azerbaijan agree with those obtained in other malaria-endemic areas. Studies in the Brazilian Amazon (Manaus) among local populations demonstrated a significant protection of vivax malaria in the G6PD-deficient men enrolled in these studies, independently of their age [68]. Extension of research in the same areas corroborated the results and inferences of previous studies and revealed that the G6PD-deficient individuals with vivax malaria were less likely to report the occurrence of malaria episodes due to the protective effect related to the enzyme activity [69, 70].

Results of studies in the areas bordering Thailand-Myanmar showed no significant difference in the malaria positivity rate when comparing G6PD normal and deficient subjects. However, although not significant, parasite densities were a few times lower in the presence of G6PD deficiency compared to densities in normal subjects for P. falciparum and P. vivax [71].

Another aspect of the problem was recently discussed by Baird [72]. It appears that the problem of G6PD deficiency excludes many people from safe and effective treatment at the latent stage of vivax malaria, such as the G6PD-deficient persons, pregnant and lactating women, young infants, people with chronic hepatic problems, and a few others. As a result, a large fraction of the population constitutes a persistent source of infection in the community, both with clinical manifestations and asymptomatic carriers. A good illustration of such a situation is malaria incidence among excluded persons from mass drug administration with primaquine in the Democratic People Republic of Korea (Table 3).
Table 3

Malaria among people excluded from the mass drug administration with primaquine, Democratic People Republic of Korea, 2002–2003 [15, 73]

County

Target population

Total excluded

% excluded

Cases among excluded

Incidence

Panmum

30,000

2100

7

18

8.6

Hwanju

74,030

10,000

13.5

147

14.7

Anbyon

40,970

4978

12.1

123

24.7

Sonchon

54,470

6793

12.5

45

6.6

Kangnam

33,030

1950

5.9

14

7.2

Suchon

86,600

10,133

11.7

114

11.2

Hwanhu

2263

542

23.9

18

33.2

TOTAL

320,763

36,495

11.38

479

13.1

Probable role of β-thalassaemia in Plasmodium vivax asymptomatic malaria

The mechanisms by which the thalassaemia protects against malaria are not known with any certainty [74]. Reduced parasite growth occurs in β-thalassaemia cells, particularly when exposed to oxidant stress. Infected cells from β-thalassaemia subjects show enhanced antigen expression at the surface of the infected red cells, possibly leading to enhanced immune clearance and further to mild attacks and an asymptomatic course of infection [74].

The highest prevalence of β-thalassaemia was found in the territory of the former USSR in Azerbaijan, where its prevalence was, on average, 8.67%, ranging from 9.28% in the lowlands to 10.16% in the foothills; both populations compose 70% of the total population of the country. The highest prevalence of 16.8% was found in one district in the foothills. Very low prevalence was established in the mountains (1000–2000 m asl): 1.30%, with 0% prevalence in the high mountains. The distribution of β-thalassaemia almost ideally coincided with the distribution of malaria in the past and in the present. Importantly, a strong correlation in the analogous geographical distribution of the G6PD deficiency had also been marked. Another important finding was that, on making a comparison with the occurrence of different forms of malaria, areas with high concentrations of the gene for β-thalassaemia did not correspond with the occurrence of P. falciparum in the past; rather, they corresponded with the occurrence of P. vivax [75, 76]. Thus, coexistence of the G6PD deficiency and β-thalassaemia among the local population in Azerbaijan might contribute to the phenomenon of asymptomatic malaria in the country, which may account for the difficulty in eliminating malaria in this country.

Asymptomatic malaria due to mixed parasitic diseases

A detailed review of published papers on the interactions between worms and malaria has led to the conclusion that, at present, no clear picture exists as to the outcome of this interaction [77]. Nevertheless, some worms, much more so than others, demonstrate protective properties against malaria and its severe manifestations. Ascaris lumbricoides is one such parasite, whereas hookworms seem to increase malaria incidence and manifestations [78]. Important epidemiological evidence transcribed from these publications indicate that nematode worms in malaria patients are capable of reducing some symptoms (e.g., body temperature) of the disease, thus precluding patients seeking treatment and thus contributing to the malaria infection remaining asymptomatic [78, 79, 80]. This inference was recently further confirmed by the results of a prospective cohort study in Mali. Co-infection with Schistosoma haematobium and P. falciparum was significantly associated with a reduced risk of febrile malaria in long-term asymptomatic carriers of P. falciparum [81].

Association of malaria with HIV infection

Malaria and HIV are major global health problems, particularly in the countries south of Sahara. Both infections share extensive epidemiological overlap, co-infecting large numbers of people in many endemic countries of the world. HIV-1 infection has long been associated with an increased frequency of clinical malaria and parasitaemia. This association became even more pronounced with advancing immunosuppression [82]. Furthermore, HIV co-infection is not only is associated with increased disease severity but also with malaria mortality in an area of stable malaria transmission with an overwhelming preponderance of P. falciparum [83]. However, recent studies in Nigeria have revealed that, under certain situations, for example, in children under 5 years of age, asymptomatic malaria was more common in HIV-positive children [84].

The situation in areas with relatively low levels of malaria transmission appeared somewhat different. The results of studies in southern India showed that P. vivax accounted for a majority of the infections (60%), followed by P. falciparum (27%) and mixed infections (13%). Plasmodium falciparum infection was more than sixfold higher among HIV-positive individuals compared with their counterparts infected with P. vivax, thus suggesting that a number of P. vivax cases could be asymptomatic cases [85].

Results of a comparative cross-sectional study in Oromi, Ethiopia, known for local transmission of both P. vivax and P. falciparum, documented lower malaria prevalence among HIV-seropositive individuals who came for routine follow-up. Clinical symptoms of malaria were more pronounced among HIV-seronegative than HIV-seropositive patients [86]. On the whole, in epidemiological settings with high malaria transmission (P. falciparum-predominant malaria), asymptomatic malaria is associated with HIV-seronegative infections. In areas with moderate and low-level malaria transmission, P. vivax asymptomatic cases are related to HIV-positive infection.

Implications for the diagnosis of asymptomatic vivax malaria

Several recently conducted studies in areas of low malaria transmission in Ethiopia demonstrated the presence of asymptomatic malaria in both P. vivax and P. falciparum, which could not be detected either by microscopy or RDT [86, 87, 88]. The results of these studies showed that malaria infections were not detected by microscopy despite more than 5% prevalence detected by nPCR (nested polymerase chain reaction: 5.2% for P. falciparum and 4.3% for P. vivax [86, 87, 88]. The inadequate sensitivity of microscopy and RDT to detect substantial sub-microscopic parasitaemia undoubtedly would affect malaria control/elimination plans of national malaria programmes aiming to eliminate malaria in the nearest future. Thus, PCR and its modifications (including nPCR, real-time PCR) have sufficient sensitivity to detect a higher number of infected subjects with low and sub-microscopic parasite densities than RDT and microscopy, potentially accelerating the achievement of malaria elimination [89].

Conclusions

Review of published data on the peculiarities of P. vivax epidemiology during the various stages of malaria elimination programmes at present and malaria eradication programmes in the past, particularly in the republics of the former USSR, revealed the southward extension of vivax malaria with long incubation, an increase of severe cases, a reduced response to certain anti-malarials, and the appearance of a large number of asymptomatic cases. One of the major problems facing malaria elimination programmes at its final stage is the difficulty in detection of cases under conditions of drastic reduction of intensity of malaria transmission. Retrospective analysis of published data in the former USSR and in other countries on the same subject have revealed various factors contributing to the problem, due to a mild course of infection, reduced level of clinical manifestations of the disease and low density of malaria parasites under a microscope. Such situations inevitably led to the occurrence of asymptomatic malaria cases, the epidemiological role of which is still underestimated in many situations. Additional contributing factors might be the presence of genetic disorders such as G6PD deficiency and β -thalassaemia, and probably some others. Malaria in association with other parasitic diseases represents another group of situations, potentially contributing to the development of asymptomatic malaria. Technical staff of national malaria elimination programmes, as well as personnel of health services, engaged in the implementation of anti-malaria activities should be aware of the problem of the detection of asymptomatic malaria. This could be done through the orientation training of the staff. Deployment of various approaches towards strengthening case detection mechanisms should be considered/introduced, including proactive case detection, use of PCR, nested PCR and other mechanisms. The need exists to further develop suitable methods of field detection of malaria asymptomatic cases.

Notes

Authors’ contributions

AVK and ENM had overall responsibility for the study, which included the study concept and prepared a first draft of the paper; EVS provided additional analysed data from the international sources; ENM, LFM, EVS and NAT participated in the analysis of the data and in the development of tables; AVK provided an overall technical editing of the paper and drafting the manuscript. All authors took part in the preparation of the final draft of the paper. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Availability of data and materials

All documents and publications in Russian are available with the Archive and Library at the Martsinovski Institute of the Sechenov University, Moscow, Russian Federation.

Ethics approval and consent to participate

Data used in the paper is not subject to ethical clearance as they form part of routine analysis of malaria control/elimination activities.

Funding

The authors received funding from Ministry of Education and Science Ref. No. RFMEFI60517X0002 for this work.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary material

12936_2018_2495_MOESM1_ESM.docx (18 kb)
Additional file 1. Malaria control and elimination in the former USSR.
12936_2018_2495_MOESM2_ESM.docx (17 kb)
Additional file 2. Plasmodium vivax fulminant malaria in the former USSR.

References

  1. 1.
    WHO. World malaria report 2016. Geneva: World Health Organization; 2016.Google Scholar
  2. 2.
    WHO. World malaria report 2017. Geneva: World Health Organization; 2017.Google Scholar
  3. 3.
    Pampana E. A textbook of malaria eradication. London: Oxford University Press; 1963.Google Scholar
  4. 4.
    Duchanina NN. Plasmodium vivax malaria with long incubation, its distribution in the USSR and epidemiological consequences. Thesis, Doctor of Medicine, Moscow, 1957: 60 (in Russian).Google Scholar
  5. 5.
    Rybalka VM, Beljaev AE, Lysenko AY. Population studies on Plasmodium vivax. 2. Distribution of manifestations of Plasmodium vivax in malaria foci. Bull World Health Organ. 1978;55:559–65 (in Russian).Google Scholar
  6. 6.
    Alekseev AN, Baranova AM. On probable relationship in mosquito vectors of malaria and increase of number of late manifestations of Plasmodium vivax. In: Orlov VS, Duchanina NN, editors. Malaria control in the USSR at present. Moscow; 1985, p. 281–5 (in Russian).Google Scholar
  7. 7.
    Sergiev VP, Baranova AM, Kouznetsov RL. Changes in the epidemiological pattern of Plasmodium vivax populations on the territory of the CIS. Giorn Ital Med Trop. 1972;1–4:33–5.Google Scholar
  8. 8.
    Baranova AM, Sergiev VP. Changes in the characteristics of manifestations of Plasmodium vivax in the CIS. Med Parazitol (Mosk). 1995;1:11–3 (in Russian).Google Scholar
  9. 9.
    Sergiev VP, Baranova AM, Arsenieva LP. Importation of malaria to the USSR from Afghanistan. Med Parazitol (Mosk). 1992;3:15–7 (in Russian).Google Scholar
  10. 10.
    Adak T, Sharma VP, Orlov VS. Studies on the relapse pattern in Delhi, India. Am J Trop Med Hyg. 1998;59:175–9.CrossRefGoogle Scholar
  11. 11.
    Kim JR, Nandy A, Maji AK, Addy M, Dondorp AM, Day NP, et al. Genotyping of Plasmodium vivax reveals both short and long latency relapse patterns in Kolkata. PLoS ONE. 2012;7:e39645.CrossRefGoogle Scholar
  12. 12.
    White NJ, Imwong M. Relapse. Adv Parasitol. 2012;80:113–50.CrossRefGoogle Scholar
  13. 13.
    Shu H, Lou S, Lin D, Fu R. Observations on hypnozoite of different isolates of Plasmodium vivax in untreated malaria. Zhongguo Ji Sheng Chong. 1995;13:185–8 (in Chinese).Google Scholar
  14. 14.
    Yang BL, Wang WJ, Wang WR, Hu HX, Li HX, Li XL, et al. Experimental studies on the biological characteristics of Plasmodium vivax in South Yunan. Zhongguo Ji Sheng Chong. 1986;4:101–4 (in Chinese).Google Scholar
  15. 15.
    Kondrashin AV, Baranova AM, Sergiev VP. Widespread use of primaquine for control of Plasmodium vivax epidemics in a population with varying degrees of G6PD deficiency. Med Parazitol (Mosk). 2010;4:29–34 (in Russian).Google Scholar
  16. 16.
    Srivastava HC, Sharma SK, Bhatt RM, Sharma VP. Studies on Plasmodium vivax relapse pattern in Kheda district, Gujarat. Indian J Malariol. 1996;33:173–9.PubMedGoogle Scholar
  17. 17.
    Asma UE, Taufiq F, Khan W. Prevalence and clinical manifestations of malaria in Aligarh, India. Korean J Parasitol. 2014;52:621–9.CrossRefGoogle Scholar
  18. 18.
    Kassirski IA, Plotnikov NN. The diseases in countries with hot climate. Moscow: Medgiz; 1964 (in Russian).Google Scholar
  19. 19.
    Tareev EM. Clinical malaria. Moscow: Medgiz; 1946.Google Scholar
  20. 20.
    Loban K, Polozok E. Le paludisme. Paris: MEDSI; 1983. p. 311.Google Scholar
  21. 21.
    Baird JK. Evidence and implications of mortality associated with acute Plasmodium vivax malaria. Clin Microbiol Rev. 2013;26:36–57.CrossRefGoogle Scholar
  22. 22.
    Douglas NM, Pontoronig G, Lampah DA, Yeo TW, Kenagalem E, Poespoprodjo JR, et al. Mortality attributable to Plasmodium vivax malaria: a clinical audit from Papua, Indonesia. BMC Med. 2014;12:217–8.CrossRefGoogle Scholar
  23. 23.
    Limaye CS, Londhey VA, Nabar ST. The study of complications of vivax malaria in comparison with Plasmodium falciparum malaria in Mumbai. J Assoc Physic India. 2012;60:15–8.Google Scholar
  24. 24.
    Naing C, Whittaker MA, Wai VN, Mak JW. Is Plasmodium vivax malaria a severe malaria? A systematic review and meta-analysis. PLoS Negl Trop Dis. 2014;8:e3071.CrossRefGoogle Scholar
  25. 25.
    Rahimi BA, Thakkinstian A, White NJ, Sirivichyaku C, Dondorp AM, Chokeijindachai W. Severe vivax malaria: a systematic review and meta-analysis of clinical studies since 1900. Malar J. 2014;13:481.CrossRefGoogle Scholar
  26. 26.
    WHO. World malaria report 2014. Geneva: World Health Organization; 2014.Google Scholar
  27. 27.
    Joshi H, Prajapati SK, Verma A, Kanga’a S, Carlton JM. Plasmodium vivax in India. Trends Parasitol. 2008;24:228–35.CrossRefGoogle Scholar
  28. 28.
    Lampah DA, Yeo TW, Malloy M, Kenangalem E, Douglas NM, Ronaldo D, et al. Severe malarial trombocytopenia: a risk factor for mortality in Papua, Indonesia. J Infect Dis. 2015;211:623–34.CrossRefGoogle Scholar
  29. 29.
    Baird K. Origins and implications of neglect of G6PD deficiency and primaquine toxicity in Plasmodium vivax malaria. Pathog Glob Health. 2015;109:91–104.CrossRefGoogle Scholar
  30. 30.
    Kochar DK, Das A, Kochar A, Middha S, Acharya J, Tanwar GS, et al. A prospective study on adult patients of severe malaria caused by Plasmodium falciparum, Plasmodium vivax and mixed infections from Bikaner, northwest India. J Vector Borne Dis. 2014;51:200–10.PubMedGoogle Scholar
  31. 31.
    Poespoprodjo JR, Fobia W, Kenangalem E, Lampah DA, Hasanuddin A, Warikar N, et al. Vivax malaria: a major cause of morbidity in early infancy. Clin Infect Dis. 2009;48:1704–12.CrossRefGoogle Scholar
  32. 32.
    Douglas NM, Anstey NM, Buffet PA, Poespoprodio JR, Yeo TW, White NJ, et al. The anaemia of Plasmodium vivax malaria. Malar J. 2012;11:135.CrossRefGoogle Scholar
  33. 33.
    Quispe AM, Pozio E, Guerrero E, Durand S, Baldeviano GC, Edgel KA, et al. Plasmodium vivax hospitalizations in monoendemic malaria region: severe vivax malaria? Am J Trop Med Hyg. 2014;91:11–7.CrossRefGoogle Scholar
  34. 34.
    Kondrashin AV, Baranova AM, Morozova LF, Stepanova EV. Actual tasks of malaria elimination. Med Parazitol (Mosk). 2011;3:3–9 (in Russian).Google Scholar
  35. 35.
    Moshkovski SD. Basic principles of malaria epidemiology. Moscow: USSR Academy of Medical Science; 1950. p. 5–11 (in Russian).Google Scholar
  36. 36.
    Duchanina NN. Distribution of P. vivax malaria with long incubation period in the USSR. In: Proceedings of Martzinovski Institute of Medical Parasitology and Tropical Medicine, 1959, Moscow, p. 103–106 (in Russian).Google Scholar
  37. 37.
    Baranova AM. Characteristics of epidemiological process of malaria in Tajik SSR under malaria control. In: Orlov VS, editor. Actual malaria problems, Ministry of Health, Kurgan Tube [Tajik SSR] 1988:63–72 (in Russian).Google Scholar
  38. 38.
    Baranova AM, Losev OL, Sabgaida TP. Peculiarities of epidemiological process in malaria foci of various types in Geokchai district of Azerbaijan SSR. In: Orlov VS, Duchanina NN, editors. Malaria control in the USSR at present. Moscow: Ministry of Health; 1985. p. 57–67 (in Russian).Google Scholar
  39. 39.
    Baranova AM. Malaria in Russia and in the CIS. Med Parazitol (Mosk). 1988;3:58–60 (in Russian).Google Scholar
  40. 40.
    Sergiev VP, Baranova AM, Sabgaida TP. Decreasing P. vivax parasitaemia in the blood films of patients in malaria residual foci. Abstracts, II European Congress of Parasitology, Paris, France, 1995:28.Google Scholar
  41. 41.
    Abdullaev HI, Davidova MA, Ahmedova AA, Djafarov AA. On activities of Central Control Laboratory of Parasitology Department of the Republican Sanitary Epidemiological Station of Azerbaijan. In: Orlov VS, editor. Actual problems of malaria. Moscow: Ministry of Health; 1988. p. 100–3 (in Russian).Google Scholar
  42. 42.
    WHO. Guidelines for the treatment of malaria. 3rd ed. Geneva: World Health Organization; 2015.Google Scholar
  43. 43.
    Sergiev PG, Yakusheva AI. Control of malaria in the USSR. Moscow: Medgiz; 1956. p. 308 (in Russian).Google Scholar
  44. 44.
    Bakradze GL, Macharadze TG. On clinical manifestations of P. vivax malaria and some parasitological changes during consolidation phase of malaria eradication in Georgia. Transactions of Virsaladze Institute of Medical Parasitology and Tropical Medicine, Tbilisi, 1965; p. 49–50 (in Russian).Google Scholar
  45. 45.
    Rabinovich SA, Kondrashin AV, Tokmalaev AK, Maksakovskaya EV, Sadikova VD, Burchik MA, et al. Peculiarities of imported P. vivax malaria caused by Chesson-like strains. Med Parazitol (Mosk). 2012;4:7–11 (in Russian).Google Scholar
  46. 46.
    Kondrashin AV. Materials and approaches to malaria stratification in relation to the rational use of antimalarial drugs in South Asia. WHO/MAL/987;1035:3–51 (in Russian).Google Scholar
  47. 47.
    Basavaraj HR. Observations on the treatment of 678 malaria cases with primaquine in an area free from malaria transmission in Mysore State, India. Indian J Malariol. 1960;14:269–81.PubMedGoogle Scholar
  48. 48.
    Sharma RC, Gautam AS, Orlov V, Sharma VP. Relapse pattern of Plasmodium vivax in Kheda district, Gujarat. Indian J Malariol. 1990;27:95–9.PubMedGoogle Scholar
  49. 49.
    Gogtay N, Carg M, Kadam V, Kamtekar K, Kshirsagar NA. A 5-day primaquine regimen an antirelapse therapy for Plasmodium vivax. Trans R Soc Trop Med. 1998;92:341.CrossRefGoogle Scholar
  50. 50.
    Adak T, Valecha N, Sharma VP. Plasmodium vivax polymorphism in a clinical trial. Clin Diagn Lab Immunol. 2001;8:891–4.PubMedPubMedCentralGoogle Scholar
  51. 51.
    Yadav RS, Ghosh SK. Radical curative efficacy of five-day regimen of primaquine for treatment of Plasmodium vivax in India. J Parasitol. 2002;88:1042–4.CrossRefGoogle Scholar
  52. 52.
    Anvikar AR, Arora U, Sonal GS, Mishra N, Shatri B, Savargaonkar D, et al. Antimalarial drug policy in India: past, present and future. Indian J Med Res. 2014;139:205–15.PubMedPubMedCentralGoogle Scholar
  53. 53.
    Rashina MG. Role of asymptomatic malaria in epidemiology of malaria. News Medicine. 1952;27:22–8 (in Russian).Google Scholar
  54. 54.
    Jukova TA, Gozodova GE, Prisyagin LA. On asymptomatic malaria carriers of P. vivax in Massali district of Azerbaijan SSR. Med Parazitol (Mosk). 1961;5:572–9 (in Russian).Google Scholar
  55. 55.
    Harris I, Sharrock WW, Main LM, Gray KA, Bobogare A, Boaz L, et al. A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting. Malar J. 2010;9:254.CrossRefGoogle Scholar
  56. 56.
    Starzengruber P, Fuehrer HP, Ley B, Thrimer K, Swoboda P, Habler VE, et al. High prevalence of asymptomatic malaria in south-eastern Bangladesh. Malar J. 2014;13:6.CrossRefGoogle Scholar
  57. 57.
    Lysenko AY, Kondrashin AV, Ejov MN. Malariology. 2nd ed. Copenhagen: World Health Organization, Regional Office for Europe; 2003.Google Scholar
  58. 58.
    Yakusheva AI. Malaria curve types and relationship spring new cases of malaria with autumn incidence. Med Parazitol (Mosk). 1939;3:262–80 (in Russian).Google Scholar
  59. 59.
    Remennikova MA. Importance of asymptomatic malaria carriers in P. vivax and P. falciparum in malaria epidemiology and magnitude of it in intensive malaria focus. Med Parazitol (Mosk). 1943;4:47–57 (in Russian).Google Scholar
  60. 60.
    Lin JT, Saunders DL, Meshnick SR. The role of submicroscopic parasitemia in malaria transmission: what is the evidence? Trends Parasitol. 2014;30:183–90.CrossRefGoogle Scholar
  61. 61.
    Laishram DD, Sutton PL, Nanda N, Sharma WL, Sobti RC, Carlton JM, et al. The complexities of malaria disease manifestations with a focus on asymptomatic malaria. Malar J. 2012;11:29.CrossRefGoogle Scholar
  62. 62.
    Howes RE, Piel FB, Patil AP, Nyangiri OA, Gething PW, Dewi M, et al. G6PD deficiency prevalence and estimates of affected populations in malaria endemic countries: a geostatistical model-based map. PLoS Med. 2012;9:1001339.CrossRefGoogle Scholar
  63. 63.
    Allison AC, Clyde DF. Malaria in African children with deficient erythrocyte glucose-6-phosphate dehydrogenase. Br Med J. 1961;1:1346–9.CrossRefGoogle Scholar
  64. 64.
    Livingstone FB. Aspects of the population dynamics of the abnormal hemoglobin and glucose-6-phosphate dehydrogenase deficiency genes. Am J Human Genet. 1965;16(4):435–50.Google Scholar
  65. 65.
    Siniscalco M, Bernini G, Filippi B, Latte P, Khan M, Piomelli S, et al. Population genetics of haemoglobin variants, thalassaemia and glucose-6-phosphate dehydrogenase deficiency, with particular reference to the malaria hypothesis. Bull World Health Organ. 1966;34:379–93.PubMedPubMedCentralGoogle Scholar
  66. 66.
    Cappadoro M, Giribaldi G, O’Brien E, Turrini F, Mannu F, Ulliers D, et al. Early phagocytosis of glucose-6-dehydrogenease (G6PD)-deficient erythrocytes parasitized by Plasmodium falciparum may explain malaria protection in G6PD deficiency. Blood. 1998;92:2527–34.PubMedGoogle Scholar
  67. 67.
    Alexeeva MI, Abrashkin-Jouckov RG, Lysenko AY, Glazunova ZI, Gorbunova YP, Mirsiyabov AU, et al. Distribution of the G6PD deficiency in Azerbaijan and its malariological significance. In: Lysenko AY, editor. Actual problems of malaria prevention in the USSR. Baku: Ministry of Health; 1973. p. 91–4 (in Russian).Google Scholar
  68. 68.
    Santana MS, de Lacerda MVG, Barbosa MD, Alecrim WD, Alecrim M. Glucose-6-phosphate dehydrogenase deficiency in an endemic area of malaria in Manaus: a cross-sectional survey in the Brazilian Amazon. PLoS ONE. 2009;4:e5259.CrossRefGoogle Scholar
  69. 69.
    Santana MS, Monteiro WM, Siqueira AM, Costa MF, Sampaio L, Lacerda MV, et al. Glucose-6-phosphate degydrogenase deficient variants are associated with reduced susceptibility to malaria in the Brazilian Amazon. Trans R Soc Trop Med Hyg. 2013;107:301–6.CrossRefGoogle Scholar
  70. 70.
    Monteiro WM, Franca GP, Melo GC, Queiroz ALM, Brito M, Peixoto HM, et al. Clinical complications of G6PD deficiency in Latin American and Caribbean populations: systematic review and implications for malaria elimination programmes. Malar J. 2014;13:70.CrossRefGoogle Scholar
  71. 71.
    Bancone G, Chu CS, Somsakchaicharoen R, Chowwiwat N, Parker DM, Charunwattana P, et al. Characterization of G6PD genotypes and phenotypes on the Northwestern Thailand-Myanmar border. PLoS ONE. 2014;9:e0116063.CrossRefGoogle Scholar
  72. 72.
    Baird JK, Battle KE, Howes RE. Primaquine ineligibility in anti-relapse therapy of Plasmodium vivax malaria: the problem of G6PD deficiency and cytochrome P-450 2D6 polymorphisms. Malar J. 2018;17:42.CrossRefGoogle Scholar
  73. 73.
    Kondrashin AV, Baranova AM, Ashley EA, Recht J, White NJ, Sergiev VP. Mass primaquine treatment to eliminate vivax malaria: lessons from the past. Malar J. 2014;13:51.CrossRefGoogle Scholar
  74. 74.
    Warrell DA, Gilles HM. Essential malariology. 4th ed. London: Arnold; 2002.Google Scholar
  75. 75.
    Mestiashvili IG. Study on the genogeography of beta-thalassemia with regards to the “malaria hypothesis”. Vestn Akad Med Nauk SSSR. 1984;51:156–265 (in Russian).Google Scholar
  76. 76.
    Voronov AA, Gordeuk VR. Genogeography of hemoglobinopathies in the USSR. In: Winter WP, editor. Hemoglobin variants in human populations. Boca Raton: CRC Press, Inc; 1987.Google Scholar
  77. 77.
    Nacher M. Interactions between worms and malaria: good worms or bad worms? Malar J. 2011;10:259.CrossRefGoogle Scholar
  78. 78.
    Nacher M, Singhasicanon P, Traore B, Dejvorakul S, Phumratanaprapin W, Looareesuwan S, et al. Hookworm infection is associated with decreased body temperature during mild Plasmodium falciparum malaria. Am J Trop Med Hyg. 2001;65:136–7.CrossRefGoogle Scholar
  79. 79.
    Nacher M, Singhasicanon P, Treeptassetsu S, Krudsood S, Gay F, Mazier D, et al. Association of helminth infections with increased gametocyte carriage during mild falciparum malaria in Thailand. Am J Trop Med Hyg. 2001;65:644–7.CrossRefGoogle Scholar
  80. 80.
    Nacher M. Helminth-infected patients with malaria: a low profile transmission hub? Malar J. 2012;1:376.CrossRefGoogle Scholar
  81. 81.
    Doumbo S, Tran TM, Sangala J, Li S, Doumtabe D, Kone Y, et al. Co-infection of long-term carriers of Plasmodium falciparum with Schistosoma haematobium enhances protection from febrile malaria; a prospective cohort study in Mali. PLoS Negl Trop Dis. 2014;9:e3154.CrossRefGoogle Scholar
  82. 82.
    Whitworth J, Morgan D, Qudley M, Smith A, Mayania B, Eotu H, et al. Effect of HIV-1 and increasing immunosuppression on malaria parasitaemia and clinical episodes in adults in rural Uganda: a cohort study. Lancet. 2000;356:105–60.CrossRefGoogle Scholar
  83. 83.
    Berg A, Patel S, Aukrust P, David C, Gonca M, Berg ES, et al. Increased severity and mortality in adults co-infected with malaria and HIV in Maputo, Mozambique: a prospective cross-sectional study. PLoS ONE. 2014;9:e88257.CrossRefGoogle Scholar
  84. 84.
    Okonkwo IR, Ibadin MO, Omoigberale AI, Sadon WE. Effect of HIV-1 serostatus on the prevalence of asymptomatic malaria Plasmodium falciparum parasitaemia among children less than 5-year of age in Benin city, Nigeria. J Pediatric Infect Dis Soc. 2016;5:21–8.CrossRefGoogle Scholar
  85. 85.
    Bharti AR, Saravanan S, Madhavan V, Smith DM, Sharma J, Balakrishnan R, et al. Correlates of HIV and malaria co-infection in southern India. Malar J. 2012;11:306.CrossRefGoogle Scholar
  86. 86.
    Alemayehu G, Melaki Z, Abreha T, Alemayehu B, Girma S, Tadesse Y, et al. Burden of malaria among adult patients attending general medical department and HIV care and treatment clinics in Oromia, Ethiopia: a comparative cross-sectional study. Malar J. 2015;14:501.CrossRefGoogle Scholar
  87. 87.
    Golassa L, Enweji N, Erko B, Aseffa A, Swedberg G. Detection of a substantial number of sub-microscopic Plasmodium falciparum infections by polymerase chain reaction: a potential threat to malaria control and diagnosis in Ethiopia. Malar J. 2013;12:352.CrossRefGoogle Scholar
  88. 88.
    Tadesse FG, Pett H, Baidjoe A, Lanke K, Grignard L, Sutherland C, et al. Submicroscopic carriage of Plasmodium and Plasmodium vivax in low endemic area in Ethiopia where no parasitaemia was detected by microscopy or rapid diagnostic test. Malar J. 2015;14:303.CrossRefGoogle Scholar
  89. 89.
    Mirahmad H, Shahrakipur A, Mehravarana A, Khorashad AS, Rahmati-Balaghaleh M, Zarean M. Evaluation of malaria multiplex/nested PCR performance at low parasite densities and mixed infection in Iran: a country close to malaria elimination. Insect Genet Evol. 2018;65:283–7.CrossRefGoogle Scholar

Copyright information

© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  1. 1.Sechenov UniversityMoscowRussian Federation
  2. 2.Department of Tropical, Parasitic Diseases and DisinfectologyRussian Medical Academy of Continuous Professional EducationMoscowRussian Federation

Personalised recommendations