Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their superior physical and mechanical properties. We engineered a reversed fatty acid β-oxidation pathway in Escherichia coli that can synthesize mcl-PHA directly from glucose and achieved high yield. However, there were only even-chain monomers in the biosynthetic polymers. The need for mcl-PHA harboring both even- and odd-chain monomers with better and wider utility impels us to develop the biosynthetic routes for the production of the novel and unnatural mcl-PHA through rewiring the basic metabolism.
In the present study, a propionate assimilation and metabolic route was integrated into the reversed fatty acid β-oxidation in order to produce mcl-PHA consisting of both even- and odd-numbered monomers. The content of odd-numbered monomers in mcl-PHA was improved with the increased propionate addition. After further deletion of pyruvate oxidase (PoxB) and pyruvate formate-lyase (PflB), the metabolically engineered chassis E. coli LZ08 harboring pQQ05 and pZQ06 (overexpression of prpP and prpE genes from Ralstonia eutropha H16) innovatively accumulated 6.23 wt% mcl-PHA containing odd-chain monomers ranging from 7 to 13 carbon atoms about 20.03 mol%.
This is the first successful report on production of mcl-PHA harboring both even- and odd-chain monomers (C6–C14) synthesized from glucose and propionate in recombinant E. coli. This present study achieved the highest yield of de novo production of mcl-PHA containing odd-numbered monomers in E. coli at shake-flask fermentation level. Continued engineering of host strains and pathway enzymes will ultimately lead to more economical production of odd-chain monomers based on market demand. The synthetic pathway can provide a promising platform for production of other value-added chemicals and biomaterials that use acetyl-CoA and propionyl-CoA as versatile precursors and can be extended to other microorganisms as intelligent cell factories.
The continuous consumption of resources such as petroleum and fossil fuels along with the increasing environmental pollution caused by petrochemical plastics have generated significant interests in developing and synthesizing bio-based materials. Polyhydroxyalkanoates (PHAs), as a class of environmental-friendly biomaterials, are accumulated by a variety of microbes from renewable carbon resources such as sugars [1, 2]. They have garnered great attention because of their unparalleled properties similar to elastomers and thermoplastics as potential alternatives for petroleum-based polymers [3, 4].
According to the different chain length monomer composition, PHAs can be divided into three main types: short-chain-length PHAs (scl-PHAs) which contain 3–5 carbon atoms, medium-chain-length PHAs (mcl-PHAs) which contain 6–14 carbon atoms, and scl-mcl PHAs which contain 3–14 carbons in length . The composition of copolymers determines the physical and mechanical material properties of the bioplastics. Generally, mcl-PHAs are synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads in nature . They are semicrystalline and thermoplastic elastomers which are suitable for the materials in biomedical application . The traditional mcl-PHAs with only even-chain monomers have shown to own a desirable set of physical properties, and incorporating the fractions of odd-numbered monomers may lend the plastics more strength and flexibility so as to endow the polyesters novel and favorable properties and utilities. The Pseudomonas putida KT2442 mutant, KTOY06, accumulated a homopolymer of poly-3-hydroxyheptanoate (P3HHp) up to 71 wt% of its cell dry weight (CDW) when heptanoate was added as a single carbon source . In another case, 3-hydroxynonanoate (3HN) monomer (30–80 mol%) was the major constituent of polyhydroxyalkanoates accumulated from odd-numbered fatty acids by microorganisms . Lately, feeding of odd carboxylic acids ranging from valeric acid to pentadecanoic acid resulted in the odd carbon number monomer fractions such as 3HHp, 3HN and 3-hydroxyundecanoate (3HUD) and a small amount (10 mol% or less) of even carbon number monomer fractions was also detected in P. putida Bet001 . Researchers also reported that in N-limited shake flasks using nonanoic acid, P. citronellolis DSM 50332 produced 32% of its dry biomass as mcl-PHA containing 78% 3HN with 22% 3HHp . Therefore, propionate or odd-chain fatty-acid-rich feedstocks have been exogenously supplemented in the culture medium for their direct conversion to propionyl-CoA as the aforementioned studies. However, the high costs and toxicity to microbial cells associated with these fatty acids will limit their practical applications. Besides, the monomer types of mcl-PHA synthesized in the above research were not diversified. In view of this, it is a pressing demand to exploit an efficient metabolic pathway that leads to the formation of corresponding odd-chain (R)-3-hydroxyacyl-CoA as precursors for the acyl-chain elongation to biosynthesize mcl-PHA containing various odd-numbered monomers via adding the inexpensive carbon source-glucose.
For the past few years, rational strategies for metabolic pathway engineering and synthetic biology were exploited to balance the enzyme expression, eliminate the pathway regulatory bottleneck, and facilitate the production of targeted metabolites [12,13,14,15,16], such as PHA production [17,18,19,20]. The engineered reversal of the fatty acid β-oxidation cycle provides a promising platform that can support the generation of various advanced products at high yields from renewable feedstocks recently with the development of systems metabolic engineering and synthetic biology [21,22,23]. Furthermore, there has been no report on the accumulation of odd-chain acyl-CoA for mcl-PHA production using glucose and propionate in E. coli cell factory by far. For this reason, the functional fatty acid β-oxidation reversal was mediated through supplying two-carbon extending acyl-CoA molecules from unrelated and cheap carbon source as biogenic precursors to synthesize different odd-numbered (R)-3-hydroxyacyl-CoA instead of adding only related carbon sources-fatty acids. To synthesize mcl-PHA that contained odd-chain monomers from the reversed fatty acid β-oxidation cycle, the starting precursor propionyl-CoA must be provided. In the previous study, after overexpressing the prpP gene in E. coli, the increasing pool of intracellular propionate facilitated the content of propionyl-CoA and increased the cell biomass . For the production of PHBV, Yang et al. employed the prpE gene from Ralstonia eutropha H16 to synthesize the propionyl-CoA and elevated the 3HV monomer fraction . Regarding PctRe, it can catalyze the transfer of CoA from acetyl-CoA to propionate . At the same time, it is worth mentioning that acetate overflow is the major drawback for production of acetyl-CoA-derived chemicals. Approaches for overcoming acetate overflow may be beneficial for biomass accumulation and the production of acetyl-CoA-derived products; for instance, PHA . This research aimed to construct the metabolic pathway for PHA production by integrating two parallel modules leading to the production of the even-chain monomers and the odd-chain monomers. The results demonstrated that the amount of odd-numbered monomers accumulated in the recombinant E. coli depended on the combination of propionate supplementation and propionyl-CoA supply. This is the first case revealing that engineered E. coli can produce novel and unnatural mcl-PHA consisting of the highest amount of odd-chain ranging from C7 to C13 motieties from glucose with addition of propionate.
Results and discussion
Construction and integration of individual module to enable direct microbial synthesis of even- and odd-chain mcl-PHA
In the previous study, we constructed an efficient even-chain mcl-PHA biosynthetic pathway in recombinant E. coli via the reversed fatty acid β-oxidation cycle . In order to supply odd-chain monomer precursors, an intracellular de novo biosynthetic module was established. For this, prpP, acs, prpE, and pct genes were overexpressed and then we constructed a series of plasmids, namely pZQ01 (pBBR1MCS2-prpP), pZQ02 (pBBR1MCS2-acs), pZQ03 (pBBR1MCS2-prpE) and pZQ04 (pBBR1MCS2-pct) as four kinds of odd-chain precursor supply module. After this, the entire pathway was built and divided into three modules: even-chain precursor supply, odd-chain precursor supply, and the reversed β-oxidation biosynthetic cycle (Fig. 1). Glucose leads to the formation of acetyl-CoA through glycolysis for the priming molecule production whereas in the engineered pathway of odd-chain precursor supply, propionate uptake can be promoted by propionate permease (PrpP) according to the previous results . Meanwhile, propionate is also directly activated by propionyl-CoA synthetase (PrpE/Acs) or propionate CoA-transferase (Pct) to incorporate a 3-hydroxyvaleryl-CoA unit into the extended polymer chain by the PHA synthase encoded by phaC2Pa from Pseudomonas aeruginosa PAO1. In light of this reason, the prpE gene encoding propionyl-CoA synthase from R. eutropha H16 was used to attach CoA group to propionic acid. Overexpression of prpP increased the substrate of PrpE to form propionyl-CoA. Additionally, Liu et al. found that deletion of acs in E. coli resulted in blockage of propionate utilization, so that Acs is essential for propionate utilization in E. coli and responsible for transforming propionate to propionyl-CoA . The propionate CoA-transferase from R. eutropha H16 (PctRe) preferably uses propionate as CoA acceptor .
After integration of the aforementioned three modules, we utilized them together to produce the even- and odd-chain mcl-PHA polyesters from glucose with supplement of extracellular propionate. To assess the effect of genes prpP, acs, prpE and pct on the odd-chain fractions in mcl-PHA production, we co-transformed the plasmids pQQ05 and pZQ01, pQQ05 and pZQ02, pQQ05 and pZQ03, pQQ05 and pZQ04, respectively, into the engineered E. coli strain LZ05 which yielded the highest content of even-chain mcl-PHA and set LZ05 containing pQQ05 (LZ05/pQQ05), previously constructed, as the control strain . After the shake flask study at 30 °C and 250 rpm by co-feeding 30 g/L glucose and 1.5 g/L propionate, the control strain LZ05/pQQ05 was able to synthesize about 4.12 wt% mcl-PHA only with even-chain monomers (Fig. 2). However, the recombinant strain LZ05 harboring the above combination of plasmids were all found to be able to accumulate even- and odd-numbered mcl-PHA with carbon chain length from C6 to C14. The results suggested that we successfully established the distinct pathway for producing targeted mcl-PHA in E. coli. Among these recombinants, the strain LZ05 harboring plasmids pQQ05 and pZQ02 accumulated the mcl-PHA approximately 2.97 wt% containing the highest amount of even-chain monomers about 90.17 mol%. Moreover, LZ05 (pQQ05, pZQ02) also produced the higher molar content of 3-hydroxyhexanoate (3HHx) fraction than that of other recombinants. Furthermore, the engineered strain LZ05 harboring plasmids pQQ05 and pZQ01 produced mcl-PHA approximately 3.21% of the CDW with the highest amount of odd-numbered mcl-PHA monomers up to 11.24 mol%, among which the 3HHp fraction in mcl-PHA was maintained at about 6.46 mol%. It indicated that a relatively higher metabolic flux was shunted towards propionyl-CoA synthesis when overexpressing prpP in the cells (Figs. 2, 3). Nevertheless, the engineered strain LZ05 harboring plasmids pQQ05 and pZQ03 resulted in about 3.96% mcl-PHA of the CDW, which was the highest PHA content compared with the other three combination of plasmids. The molar ratios of 3HHx, 3HHp, 3-hydroxyoctanoate (3HO), 3HN, 3-hydroxydecanoate (3HD), 3HUD, 3- hydroxydodecanoate (3HDD), 3-hydroxytridecanoate (3HTRD) and 3-hydroxytetradecanoate (3HTD) were approximately 0.76, 5.91, 48.60, 2.82, 22.49, 0.98, 11.74, 0.69 and 6.01, respectively. In total, it had the odd-chain monomers with a molar content of 10.40% (Figs. 2, 3). Herein, it can be concluded that prpP increased odd-numbered fraction formation rate and stimulate the cells to use more propionate for odd-chain fraction yield, although it may not accumulate the highest amount of mcl-PHA polyesters. Regarding the cell growth phenomena, LZ05 harboring plasmids pQQ05 and pZQ02 had the best performance and reached the cell dry weight of 6.80 g/L, while the CDW of the control strain LZ05/pQQ05 could only reach about 5.79 g/L. As for the composition of mcl-PHA, except the strain LZ05 (pQQ05, pZQ04) harboring mainly 3HD fraction, the other three constructed strains are all composed primarily of 3HO monomer (Figs. 2, 3). These results demonstrated that genes prpP, acs, prpE and pct had diverse impacts on the mcl-PHA production. Overexpression of prpP gene indeed enhanced the transport efficiency of propionate and offered more precursors for propionyl-CoA. Although overexpressing prpE gene accumulated the odd-chain monomers and obtained the relatively higher 3HHp and 3HN content, the incorporated odd-chain fraction was still rather low. In addition, when we overexpressed pct in pZQ04, 10.36 mol% of odd-chain monomers were detected. This is most likely because Pct harnessed acetyl-CoA and propionate as substrates to form propionyl-CoA, resulting in precursors for initiation of the odd-chain monomer biosynthesis. According to these experimental results, the strains still did not have sufficient molecules for initiation the formation of odd-chain monomers.
Improvement of odd-chain monomer biosynthesis by simultaneous overexpression of double genes in the metabolic pathway
To increase the mcl-PHA production and possess more odd-numbered monomers in recombinant E. coli, we rebuilt the biosynthetic pathway by construction of the plasmids pZQ05, pZQ06 and pZQ07. In the above plasmids, prpP and acs, prpP and prpE, and prpP and pct were simultaneously overexpressed separately, due to the highest gain of odd-numbered monomers when overexpression of prpP. Then, they were all co-transformed with pQQ05 into the strain LZ05 to form LZ05 (pQQ05, pZQ05), LZ05 (pQQ05, pZQ06), and LZ05 (pQQ05, pZQ07). The shake flask study was conducted at 30 °C and 250 rpm with addition of 30 g/L glucose and 1.5 g/L propionate. Cultivation of these strains and the control strain LZ05/pQQ05 showed that they exhibited diverse growth phenomena and different accumulation of mcl-PHA with odd-numbered monomers. The CDW of LZ05 harboring double plasmids pQQ05 and pZQ05 reached approximately up to 6.90 g/L and the cellular dry weight of LZ05 harboring double plasmids pQQ05 and pZQ06 (or pZQ07) reached about 6.69 g/L (or 6.31 g/L). Nevertheless, the CDW of LZ05/pQQ05 could reach 6.62 g/L when we simply added 30 g/L glucose for the shake flask study under the same conditions with this research in our previous work . In order to verify the effect of the single gene or three gene combination (prpP and acs, prpP and prpE, and prpP and pct) to the cell growth, statistical analyses were carried out. The results of significance tests denoted the significant difference in the CDW between LZ05 (pQQ05, pZQ06) and the control strain LZ05/pQQ05 (P < 0.05, Fig. 2). Moreover, it also demonstrated that co-overexpression of prpP and acs restored the cell growth compared with that of the strain LZ05/pQQ05 without propionate addition (6.62 g/L). As a result, overexpressing prpP and acs not only offset the toxicity of propionate to cells, but also balanced the carbon flux between cell growth and PHA synthesis. With regard to the content of mcl-PHA, they had different reflection in different strains. LZ05 (pQQ05, pZQ06) accumulated 4.96 wt% mcl-PHA polymers which is the highest among the aforementioned three strains and resulted in 1.2 fold (P < 0.05) increase in mcl-PHA production compared to the control strain. Similarly, the content of mcl-PHA was also enhanced by 1.6-fold (P < 0.001), 1.7-fold (P < 0.0001), 1.3 fold (P < 0.05), and 1.5-fold (P < 0.001) compared with LZ05 (pQQ05, pZQ01), LZ05 (pQQ05, pZQ02), LZ05 (pQQ05, pZQ03), and LZ05 (pQQ05, pZQ04), respectively. Nevertheless, the molecular content of odd-numbered fractions in LZ05 (pQQ05, pZQ06) of about 10.98 mol% was lower than LZ05 (pQQ05, pZQ01). Reasons for this phenomenon could be attributed to two points: the first one is the proper expression level of prpE is vital for its high activity to obtain high content of odd-numbered fractions. It has been reported that the expression of prpE from Salmonella enterica at a relatively high level led to decrease on 3HV content in the PHBV . The second one is whether the substrate of PrpE-propionate addition is sufficient or not. It is important to engineer host strains to provide enough precursors of specific HA-CoAs with PHA synthase for efficient production of mcl-PHA containing corresponding monomer constituents. To verify this, we attempted to supplement different concentrations of propionate in the subsequent research. Additionally, increasing total amount of mcl-PHA heterogeneous polymers was detected in the two strains LZ05 harboring pQQ05 and pZQ05, and LZ05 containing pQQ05 and pZQ07 which accounted for 4.01 wt% and 4.28 wt%, respectively. Compared with LZ05 (pQQ05, pZQ01) and LZ05 (pQQ05, pZQ04), 3HN and 3HUD fractions were augmented up to 2.77 mol% and 1.27 mol% after both overexpression of prpP and pct in the strain LZ05 (pQQ05, pZQ07) (Figs. 2, 4). This study also demonstrated that genetically modulating propionyl-CoA metabolism can be potentially applied to tailor the monomer fraction of the PHA copolymer and have preferable properties for various applications.
Effect of propionate concentration on odd-chain monomer production
In order to enhance the content of the odd-numbered fractions in the strain LZ05 harboring pQQ05 and pZQ06, propionate of various concentrations was successively added to the medium to examine the effects. The results showed that the addition of propionate was essential for the production of odd-numbered monomers in mcl-PHA copolymers, and also able to substantially promote the proportion of odd-numbered monomers in mcl-PHA copolymers with the most suitable propionate concentration. On one hand, when the concentration of propionate in the medium increased from 0 to 2.0 g/L, the proportion of odd-numbered monomers in the mcl-PHA increased from 0 to 15.30 mol%, on the other hand, the proportion of even-numbered monomers in the mcl-PHA decreased from 100 to 84.70 mol%. The concentration of mcl-PHA and CDW were approximately 5.12 wt% and 6.49 g/L, respectively, when the concentration of propionate was 2.0 g/L. However, when the concentration of propionate in the medium increased to 3.0 g/L, the proportion of odd-numbered monomers in the mcl-PHA decreased to 14.15 mol%. Meanwhile, the concentration of mcl-PHA and biomass decreased to 4.88 wt% and 6.12 g/L (Fig. 5a, b). This suggested that the high concentration of propionate inhibited cell growth, leading to a reduction of odd-numbered monomer content and total mcl-PHA production. Therefore, the optimal concentration of propionate in the medium was 2.0 g/L. With this concentration, the monomer content of LZ05 (pQQ05, pZQ06), 3HHp, 3HN, 3HUD and 3HTRD fractions were all enhanced to 7.42 mol%, 3.88 mol%, 2.20 mol% and 1.80 mol%, respectively. As a whole, not only was the engineered E. coli LZ05 (pQQ05, pZQ06) capable of obtaining the highest odd-numbered fraction content, but the total mcl-PHA content in the cell dry weight was also promoted.
Improvement of mcl-PHA accumulation by reinforcing acetyl-CoA supply
Acetyl-CoA is a key molecule in microbial central carbon metabolism and is the direct initial molecule taking part in mcl-PHA biosynthetic pathway, so enhancement of acetyl-CoA supply shall promote the mcl-PHA formation. When glucose is excess in the medium, E. coli cells do not fully activate TCA cycle, and thus generate several by-products, such as acetate . The acetate secretion not only caused low pH and was toxic to cell growth, but also decreased acetyl-CoA pool and the formation of target products . There are two main routes which induce acetate formation from pyruvate in E. coli: (1) phosphotransacetylase (pta) and acetate kinase (ackA) catalyze acetyl-CoA to acetate; (2) pyruvate oxidase (poxB) catalyzes pyruvate to acetate . In the previous study, Rhie et al. reported that deletion of ackA and pta exhibited substantially reduced levels of 3HV formation and thus would reduce the content of other odd-chain monomers. Hence, the ackA and pta genes were also confirmed to be essential for odd-chain monomer production . Additionally, the enzyme pyruvate oxidase (PoxB) is regarded as a major candidate responsible for catalyzing the decarboxylation of pyruvate to form acetate and CO2. Several approaches have been performed to reduce the competitive acetate flux through the PoxB pathways  and to increase the acetyl-CoA pool . Therefore, the poxB gene was deleted to reduce acetate secretion and provide more acetyl-CoA precursors for mcl-PHA biosynthesis in E. coli. Previous studies have indicated that intracellular ATP concentration was 18% higher in a pflB mutant growing under aerobic conditions, compared to the parental strain , which may be beneficial for biomass production and protein expression. According to this, gene deletion of pflB encoding pyruvate formate-lyase was also performed. After the double knockout, we constructed the engineered strain LZ08, and then the plasmids pQQ05 and pZQ06 were introduced into LZ08. As a consequence, when cultivation of the strain with the supplement of 30 g/L glucose and 2 g/L propionate, higher content of the mcl-PHA polymers was detected at 6.23 wt% with the molar content of odd-numbered monomers accounting for 20.03 mol% in batch cultivation, which means 456.04 mg/L mcl-PHA in the culture medium. The dominant one in even-chain monomers is also 3HO and 3HHp fraction accounts for the highest amount in odd-numbered monomers (Fig. 6a, b). Compared to other strategies such as fatty acid β-oxidation and de novo biosynthesis, the yield of mcl-PHA containing odd-numbered monomers was the highest and the category of monomer composition accumulated in a single strain was more various. These characteristics of monomer composition distribution may endow the mcl-PHA polyesters with novel mechanical properties and broader applications. Although further deletion of poxB and pflB in the engineered strain enhanced the odd-numbered monomer production, the even-numbered monomers still were the major constituents (Fig. 6a). It seemed that the Km for propionyl-CoA of thiolase (YqeF) was much higher than that of acetyl-CoA. As shown in Fig. 6b, we investigated the cell growth performance and glucose consumption of the strain LZ08 harboring pQQ05 and pZQ06. Cultivation of this engineered strain, we found that there was a short growth lag phase at the initial stage. After 10 h, the strain grew rapidly and reached its maximum cell dry weight of 7.32 g/L at 64 h (Fig. 6b). Meanwhile, we detected the acetate secretion in the medium in the shake flask experiments. As shown in Fig. 6c, acetate secretion dropped by 56% after the double deletion from 4.18 g/L to 1.84 g/L in the culture of the strain LZ08 (pQQ05, pZQ06) and the statistical analysis showed the marked decrease of acetate secretion compared with the strain LZ05 (pQQ05, pZQ06). This indicated that the double knockout was of great significance for cell growth and mcl-PHA accumulation in cells. Further studies will be needed to enhance content of the odd-numbered monomers in the mcl-PHA production and augment the biosynthesis of intracellular propionyl-CoA from renewable and structurally-unrelated carbon source directly.
It has been a challenging task to synthesize mcl-PHA copolymers for a long time, especially for synthesizing even- and odd-chain mcl-PHA monomers equal to or longer than C8. There was no research reported that could make PHA copolyesters consisting of C6–C14 even- and odd-chain monomers. However, the fatty acid β-oxidation reversal was successfully utilized to generate the intermediates of mcl-PHA from renewable feedstocks in this study. By integrating two parallel precursor-supplying modules, the E. coli strain was confirmed to produce mcl-PHA containing both odd- and even-chain monomers efficiently. After optimization of the odd-numbered monomer module and the chassis, E. coli was found to synthesize mcl-PHA up to 6.23 wt% harboring odd-numbered monomers about 20.03 mol% from glucose and propionate. To the best of our knowledge, this is by far the first report on the novel mcl-PHA production both with even- and odd-numbered monomers with the highest yield. When grown on glucose and other related fatty acids, the recombinant E. coli was capable of producing other molar ratios of the monomers. This allows for generation of more and more PHA smart materials with diverse properties. Therefore, the engineered E. coli will be recruited as potential valuable and intelligent cell factories for industrial production to meet various applications.
Microbial strains and media
Table 1 lists the various strains and plasmids used in this study. The E. coli LS5218 strain [fadR, atoC(Con)], which constitutively expresses the enzymes of fatty acid β-oxidation pathway, allows expression of various pathway genes cloned into pTrc99a and pBBR1MCS2 vectors upon induction with isopropyl-β-d-1-thiogalactopyranoside (IPTG) . The IPTG was added when the cells had reached an optical density at a wavelength of 600 nm (OD600) of 0.6. The PHA-producing strain LZ05 with deletion of the genes ptsG, tesB and yciA was described previously . The E. coli DH5α strain served as the host strain for subsequent construction and propagation of various PHA-producing plasmids. During the recombinant plasmid construction, strains were cultivated in Luria–Bertani (LB) medium (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl). For gene knockout, SOB medium (20 g/L tryptone, 5 g/L yeast extract, 0.5 g/L NaCl, 10 mM MgCl2 and 2.5 mM KCl) was utilized.
For the even-chain monomer supply, the construction of plasmid pQQ05 has been previously described . Briefly, the genes yqeF, fadB, phaJ1Pa, ter and phaC2Pa were all cloned and ligated into the corresponding sites of pTrc99a which were cut with the same restriction enzymes stepwise to generate plasmid pQQ05.
The construction of odd-chain monomer generation pathway was as follows. The codon-optimized prpP gene was cloned into the pBBR1MCS2 vector between the KpnI and BamHI sites to construct the plasmid of pZQ01. Later, in order to form the plasmid pBBR1MCS2-acs, namely pZQ02, the acs gene amplified via polymerase chain reaction (PCR) using E. coli MG1655 genomic DNA (gDNA) as template was also inserted into the pBBR1MCS2. The prpE and pct fragments amplified from R. eutropha H16 gDNA with primers prpE-F/prpE-R and pct-F/pct-R were separately ligated into the pBBR1MCS2 to yield the plasmids pZQ03 and pZQ04. Subsequently, co-expression of two genes prpP and acs, prpP and prpE, prpP and pct in the pBBR1MCS2 was utilized to form the plasmids pZQ05, pZQ06 and pZQ07, respectively. All of the genes were under the control of the lac promoter with separated ribosomal binding site located upstream of each gene to facilitate the translation. The R. eutropha H16 template used for these PCR reactions was isolated using the TIANamp Bacterial DNA Kit (TIANGEN BIOTECH, China). The primers used to amplify different fragments for cloning reactions are listed in Additional file 1: Table S1.
In all cases, PCR was performed using an S1000 Thermal Cycler (Bio-Rad, USA). PrimeSTAR HS DNA polymerase was purchased from Takara (Tokyo, Japan), restriction endonucleases were from Fermentas/Thermo Scientific (Pittsburgh, USA), and T4 DNA ligase was from New England Biolabs (Ipswich, USA). Propagated plasmids were prepared by TIANGEN Plasmid Mini Extraction Kit (TIANGEN BIOTECH, China), and restriction enzyme-digested products were purified using an E.Z.N.A.™ Gel Extraction Kit (Omega, USA). DNA sequencing of all constructed plasmids were performed by Liuhe BGI Tech Co. Ltd (Beijing, China). All of the constructed plasmids were transformed into the strain LZ05 and the optimum double plasmids were then transformed into the strain LZ08 according to standard procedures .
The gene pflB which encodes pyruvate formate lyase was knocked out by the one-step inactivation method as described previously  and poxB encoding pyruvate oxidase was knocked out by linearized DNA fragments with extending homologous sequence . First, the linerized DNA fragments with the FLP recognition target sites and 39 bp homologous sequences were obtained via PCR using pKD4 (KmR) as a template and pflB-F/pflB-R as primers. After the DNA gel extraction, the purified PCR product was electroporated into the host cells which carried the plasmid pKD46, and then E. coli LZ05 was induced by 0.3% (w/v) l-arabinose to express the λ Red system. The positive transformants were selected and identified by colony PCR using the primers pflB-test-F/pflB-test-R. Regarding the poxB deletion, primers poxB-F/poxB-R and chromosomal DNA of the strain QZ1111 were applied to amplify the linearized DNA fragments for poxB. The deletion procedure of poxB gene was as follows. After DpnI digestion, the PCR products were then purified and electroporated into the competent strain E. coli LZ05 containing the plasmid pKD46. Transformant cells were selected in solid LB medium (1% tryptone, 0.5% yeast extract, 1% NaCl, and 1.5% agar powder) containing chloramphenicol (CmR). Candidate clones were screened by PCR employing primers poxB-F/poxB-R. The PCR products were ultimately sequenced in Liuhe BGI Tech Co. Ltd (Beijing, China) if necessary. After removing pKD46, the corresponding KmR or CmR cassette was removed with the helper plasmid pCP20. The plasmids pKD46 and pCP20 were eliminated by overnight cultivation at 42 °C. Finally, the strain LZ08 with the above two gene inactivation was generated.
The medium of shake flask study contains 10 g/L tryptone, 5 g/L yeast extract, 30 mM NH4Cl, 5 mM (NH4)2SO4, 1.48 mM Na2HPO4, and 100 μM FeSO4 supplemented with 125 mM MOPS.
For all shake flask experiments, single colony was inoculated into 5 mL LB broth and grown at 37 °C overnight. 0.5 mL pre-culture was inoculated to 300 mL Erlenmeyer flask containing 50 mL LB and cultivated for 8 to 10 h and then 1% (v/v) seed inoculum for shake flask cultivation was incubated in 50 mL fermentation medium. When all liquid fermentation medium (50 mL) was incubated in 300 mL conical flasks at 37 °C with an agitation of 250 rpm to an optical density at 600 nm (OD600) of 0.6–0.8, 1 mM IPTG was added to the culture broth as an inducer. After induction, 30 g/L glucose was supplied as the sole carbon source at the appropriate time and then fermented for 72 h at 30 °C with shaking at 250 rpm. When necessary, ampicillin (100 μg/mL), kanamycin (50 μg/mL) or chloramphenicol (25 μg/mL) was added to the medium to maintain the stability of the plasmids. After cultivation, cells were gathered by centrifugation at 12,000 rpm for 15 min, washed with water twice and treated with ethanol once and then lyophilized.
PHA production analysis
The content and monomer compositions of intracellular accumulated PHA were analyzed by gas chromatography (GC) as described previously . PHA content was defined as the percent ratio of PHA concentration to CDW. Liquid culture was centrifuged to obtain the supernatant and cellular biomass. 15 mg lyophilized cells were subjected to methanolysis in the presence of 1 mL of chloroform and 1 mL of 3% (v/v) sulfuric acid in methanol for 1 h at 100 °C. The samples were cooled to room temperature and then 1 mL of distilled water was added in order to extract the cell debris that is soluble in the aqueous phase. 10 mg/mL pentadecanoic acid in ethanol was added as an internal standard. The mixture was vortexed and centrifuged at 12,000 rpm for 10 min. After the layer separation, the organic (chloroform) phase (500 μL) was transferred to another new vial and analyzed using a Shimadzu GC2010 gas chromatograph (Kyoto, Japan) equipped with an AOC-20i auto-injector and a RestekRxi®-5 column. PHA standard samples were dissolved in chloroform and also analyzed according to the method above by GC. The temperature program used was as follows: 80 °C hold for 1 min, ramp from 60 to 230 °C at 10 °C per min and a final hold at 230 °C for 10 min .
Cell growth, glucose consumption and acetate assimilation analyses
Cell growth was monitored by measuring OD600 utilizing a spectrophotometer (Shimazu, Japan). Glucose and acetate were quantitatively analyzed by high-performance liquid chromatography (HPLC) (Shimazu, Japan) which equipped with a refractive index detector (RID-10A) and an Ion Exclusion column (Bio-Rad, HPX-87H). The samples were first centrifuged at 12,000 rpm for 10 min, and then the supernatant was filtrated with a 0.22 μm filter membrane. 5 mM sulfuric acid was utilized as the mobile phase of HPLC with the flow rate of 0.6 mL/min and the utilized column temperature was 65 °C.
All data examined were expressed as mean ± SD. Statistical analyses of the data were carried out using two-tailed Student’s t-test between two groups, and one-way ANOVA followed by the post hoc Tukey’s test for multiple groups. P < 0.05 was considered significant. The * denotes P < 0.05, the *** denotes P < 0.001.
Availability of data and materials
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cell dry weight
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This research was financially supported by the grants from the National Natural Science Foundation of China (21808114), the Key Research and Development Program of Shandong Province (2019GSF107044), the Natural Science Foundation of Shandong Province (ZR2016CB03), and the Young Doctoral Cooperation Fund Project of Qilu University of Technology (Shandong Academy of Sciences) (2017BSHZ007).
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Zhuang, Q., Qi, Q. Engineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers. Microb Cell Fact 18, 135 (2019). https://doi.org/10.1186/s12934-019-1186-x
- Escherichia coli
- Odd-chain monomers
- Reversed fatty acid β-oxidation cycle
- Metabolic engineering
- Synthetic biology