Effective treatment of apatinib in desmoplastic small round cell tumor: a case report and literature review
Desmoplastic small round cell tumor (DSRCT) is a rare malignant sarcoma with poor prognosis due to lack of effective treatments. Apatinib is a new potent oral small-molecule tyrosine kinase inhibitor, and targets the intracellular domain of vascular endothelial growth factor receptor 2 (VEGFR-2). In this study, we presented a case of intra-abdominal DSRCT which was effectively treated by apatinib.
A 32-year-old man was admitted due to increasing urination frequency and palpable mass in right lower abdomen for 2 months. The mass was resected and diagnosed DSRCT. The patient refused chemotherapy and radiotherapy,and used Chinese medicine only. Six months after the surgery, the patient re-hospitalized due to growing abdominal mass and ascites. Intraperitoneal cisplatin treatment showed little effect. Apatinib was then recommended. Apatinib revealed outstanding effect on reducing mass size and ascites during 2-month treatment. Apatinib therapy continued for additional 2 months, and the patient was in good condition. The only toxicity was hand-food syndrome, which was controllable and well tolerated.
It is the first report that apatinib is effective on DSRCT. This report may provide an additional option for the treatment of metastatic DSRCT.
KeywordsApatinib Desmoplastic small round cell tumor VEGFR-2
Carbohydrate antigen 125
Desmoplastic small round cell tumor
Epithelial membrane antigen
Hyperthermic intraperitoneal chemotherapy
Intensity-modulated radiation therapy
Non-small-cell lung cancer
Peripheral neuroectodermal tumours
tyrosine kinase inhibitor
Desmoplastic small round cell tumor (DSRCT) is a rare malignant and aggressive tumor. Only 850 such patients were reported in the medical literature . DSRCT was first described by Gerald and Rosai in 1989 [2, 3]. It mainly occurs among children and young adults. No standardized treatment guideline is available nowadays. Current treatment consists of surgical resection combined with chemotherapy, radiotherapy . Although these multimodel therapies, DSRCT still has a poor prognosis, with less than 30% three-year survival rate and only 18% five-year survival rate [5, 6]. Thus, novel therapy is required.
Apatinib (Hengrui Pharmaceutical Co., Ltd., Shanghai, China) is a small molecule tyrosine kinase inhibitor (TKI) and targets vascular endothelial growth factor receptor 2 (VEGFR-2). Apatinib has been proved to be effective and safe in advanced gastric cancer, metastatic breast cancer, esophageal cancer, and non-small-cell lung cancer. Moreover, the drug has shown a substantial potential in multiple solid tumors . However, there is no any report for apatinib in treating DSRCT to day. In this study, we presented a case of intra-abdominal DSRCT, which was effectively treated by apatinib.
On October 25, the patient was given laparotomy. There were 3 masses between bladder and rectum with firm consistency and gray-white color. The sizes were about 7 × 6 × 5 cm, 8 × 7 × 4 cm, and 6 × 6 × 5 cm. No invasion was found in diaphragm, liver, spleen and pancreas. Dozens of metastatic nodules (0.5 × 0.5 × 1 ~ 5 × 2 × 1 cm) were found on the surface of omentum and mesentery of small and big intestine. All neoplasms were resected and sent for pathological evaluation.
After surgery, the patient had a good recovery, and left hospital on November 4, 2016. The patient received traditional Chinese medicine therapy instead of chemotherapy and radiotherapy because of concerns of the toxicities such as vomiting, nausea, headache, etc.
On June 4, 2017, apatinib was administered at a dose of 500 mg/d. After one-month therapy, serum CA125 level was reduced to 60.2 U/mL. Symptoms were significantly relieved and body weight and abdominal circumsference were decreased from 66 kg to 50 kg. CT scan showed abdominal ascites diminished significantly (Fig. 4b-c). After one more month of apatinib treatment, serum CA125 level was decreased to normal level (25.3 U/mL). CT scan revealed abdominal mass was further reduced (Fig. 4d). The toxicity the patient experienced was hand-food syndrome. The patient continued to use apatinib as maintenance therapy. Until now, apatinib therapy continued for additional 2 months, and the patient was in good condition.
Discussion and Conclusions
DSRCT is an extremely rare and highly aggressive malignant neoplasm. Estimated incidence is 0.2~ 0.5 per million people . It typically presents as a large intra-abdominal mass with numerous small peritoneal implants . Extra abdominal DSRCT is rare. However it was reported that some DSRCTs originated from soft tissues and bone , testis , nasal cavity , pleural cavity , uterus , kidney , lung  and skull . Liver and lung are the most two common sites of metastases . DSCRT also metastasizes to lymph nodes of the groin, neck, or mediastinum .
Clinical manifestations of DSRCT are nonspecific, including abdominal pain, distention, constipation, bowel obstruction, nausea, ascites, and compression symptoms of urinary organs such as urinary disorders. Physical examination may reveal a palpable mass in the abdominal or pelvic cavity. Ultrosonic, CT scan and MRI may reveal multiple tumor nodules.
Microscopically, DSRCT is characterized by nests of small round blue cells separated by desmoplastic stroma which shares features with other round cell tumors such as small cell carcinoma, mesothelioma, Wilms’ tumor, and Ewing’s sarcoma/peripheral neuroectodermal tumors (PNET). The gold standard for the diagnosis of DSRCT is the results of histopathology and cytogenetics. DSRCT simultaneously expresses epithelial (CK and EMA), mesenchymal (vimentin and desmin), and neural (CD56 and NSE) markers . Almost all DSRCTs are positive to WT-1 . Cytogenetic study shows unique (11:22), (p13:q12) translocation which resulted in an active fusion protein involving the Ewing sarcoma (EWS) and Wilms tumor (WT1) genes . The presence of this translocation provides definitive diagnosis for DSRCT .
The treatment of DSRCT remains challenging. No standard treatment regimen is available nowadays. Current therapies mainly include surgical therapy, chemotherapy, and radiotherapy . Primarily surgical approach is the optimal scheme. It has been reported that 3-year survival rate in cases with complete tumor resection was 58%, while the number was 0% in nonresectable cases . However, in patients with extraperitoneal metastasis, surgery can not produce any benefit . Owing to peritoneal implantation, microscopic residual disease is often present even after resection. Hyperthermic intraperitoneal chemotherapy (HIPEC) with cisplatin was considered as an effective adjunctive therapy . Currently the most accepted chemotherapy was reported by Kushner et al.  in 1996, and the alkylating agents (cyclophosphamide or ifosfamide, along with vincristine and doxorubicin alternating with ifosfamide and etoposide) were similar to those used in Ewing sarcoma. In 2009, Mora et al. published a series of pediatric cases treated with gemcitabine and docetaxel in relapse therapy . The survival benefiting from chemotherapy still outweighs its side effects. Multiple agent chemotherapy is still recommended with aggressive surgery in DSRCT. Radiation therapy in DSRCT is controversial. Goodman observed significant hematological and gastrointestinal toxicities in whole abdominopelvic radiotherpy . While intensity-modulated radiation therapy (IMRT) which was used in the adjuvant setting was beneficial to survival . Immunotherapy of DSRCT is under investigation. About 96% of DSRCT cells express neoantigen B7H3, which is targeted by monoclonal antibody 8H9. A phase I trial of intraperitoneal radioimmunotherapy with 131I-8H9 is in process in patients with DSRCT, which showed a good toleration .
Magnan et al. reported that VEGFR-2 is overexpressed in DSRCT and that DSRCT xenografts have been shown to be highly responsive to anti-VEGF agents such as bevacizumab . Small molecule TKIs including sunitinib and pazopanib have shown effective in treating DSRCT in some cases [28, 29, 30]. These results suggest that small molecule TKIs may block VEGFR and their downstream pathways, which might represent a possible treatment option for advanced DSRCT patients.
In this case, the patient refused to receive chemotherapy at initial treatment and the effect of intraperitoneal chemotherapy with cisplatin at second hospitalization was not obvious. Gene expression analysis showed the patient expressed high level of VEGFR-2. Targeted therapy was then recommended. However, no list of sunitinib and pazopanib in domestic, so we chose apatinib as a third choice.
Apatinib (YN-968D1) is one of the latest small-molecule TKIs targeting VEGFR-2. Some encouraging clinical data have shown that apatinib is effective in treating a variety of solid tumors . However, it was not reported whether apatinib exerts effects in the treatment of DSRCT. Apatinib was successfully applied as a third or fourth line drug in advanced gastic cancer and was approved and launched in China in 2014. It was also currently tested in phase II and III clinical trials in non-small cell lung cancer, breast cancer, hepatocellular carcinoma and colorectal cancer . Hypertension, proteinuria and hand-foot syndrome are the most common adverse effects.
In the present study, the patient developed abdominal metastasis and extensive ascites 8 months after surgery. Apatinib effectively relieved the symptom and reduced ascites and the mass size. The survival time has been over 4 months since the treatment. A longer-term efficacy of apatinib can be expected. Additionally, the patient suffered no severe toxicity except for hand-foot syndrome, which was controllable and well tolerated.
To the best of our knowledge, this is the first report for apatinib in treating DSRCT. High level of VEGFR-2 expression in DSRCT and succefully treatment with sunitinib and pazopanib for DSRCT provide mechanism support for the treatment. Further prospective trials are needed to further confirm the efficacy and safety of apatinib in the clinical treatment of DSRCT.
The authors thank the patient for his participation and his agreement to publication of the report.
No source of funding to declare.
Availability of data and materials
The datasets generated during the current study are not publicly available because of patient privacy, but are available from the corresponding author on reasonable request.
CS conceived the idea for this case report, and performed the surgery with YF. YF performed the surgery. LZ made the diagnosis. DD and MY followed the patients with author. LP wrote the manuscript. All authors checked and approved the final manuscript.
Ethics approval and consent to participate
Consent for publication
Written informed consent was obtained from the patient for publication of this case report and any accompanying images.
The authors declare that they have no competing interests.
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