Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content
Rapeseed (Brassica napus, B. napus) is an important oil seed crop in the world. Previous studies showed that seed germination vigor might be correlated with seed oil content in B. napus, but the regulation mechanism for seed germination has not yet been explained clearly. Dissecting the regulation mechanism of seed germination and germination vigor is necessary.
Here, proteomic and genomic approaches were used to analyze the germination process in B. napus seeds with different oil content. The identification of 165 differentially expressed proteins (DEPs) in the germinating seeds of B. napus with high and low oil content was accomplished by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE). The comparative proteomic results revealed that seeds with high oil content had higher metabolic activity, especially for sulfur amino acid metabolism. Thirty-one unique genes were shown to be significantly changed during germination between the seeds with high and low oil content, and thirteen of these genes were located within the confidence interval of germination-related quantitative trait locus (QTLs), which might play an important role in regulating seed germination vigor.
The present results are of importance for the understanding of the regulation mechanism for seed germination vigor in B. napus.
KeywordsBrassica Seed germination vigor 2D-DIGE QTL analysis
Two-dimensional fluorescence difference in gel electrophoresis
Biological variation analysis
- CBB R-250
Coomassie brilliant bule R-250
Differentially expressed proteins
Differential in-gel analysis
Ethylene Diamine Tetraacetic Acid
Hour after imbibition
High oil content
High performance liquid chromatography
Heat shock protein
Immobilized pH gradient
Late embryogenesis abundant protein
Low oil content
Polymerase Chain Reaction
Peptide mass fingerprinting
Quantitative trait locus
Sodium dodecyl sulfate
Tricarboxylic acid cycle;
Transmission electron microscopy
Seed germination is a critical development process in the life cycle of spermatophyte. Successful seed germination and normal seedling construction are the decisive factors for plant reproduction [1, 2]. Thus, research on the regulation mechanism of seed germination is important for agricultural production.
Brassica napus is the second most important oilseed crop in the world, which occupies approximately 13–16% of the world vegetable oil production . The seed germination and germination vigor are affected by many factors in B. napus, such as salt [4, 5], temperature [6, 7], plant hormones , and aging . The seed germination vigor of rapeseed could be enhanced by priming [10, 11]. In addition, a genome-wide association analysis showed that seed germination and radicle growth were strongly environment-dependent in B. napus . Conditions during seed production and storage have a profound effect on seed vigor, and the performance of seed dormancy was related to differences in germination performance . A number of genes, proteins or QTLs that correlated with germination were identified , and these genes included SNOWY COTYLEDON 1 (Bna.SCO1), ARABIDOPSIS TWO-COMPONENT RESPONSE REGULATOR (Bna.ARR4) and ARGINYL-t-RNA PROTEIN TRANSFERASE 1 (Bna.ATE1). The homologous genes in Arabidopsis have been shown to participate in seed germination and seedling growth [13, 14, 15]. A 90 kD heat shock protein (HSP 90) was detected in the elongating axes and cotyledons of germinating seedlings and could be induced by stress in B. napus, which highlights a role for HSP 90 during seed germination . Jian et al. identified 19 QTLs that are associated with seed germination rates under salt and drought stress in B. napus .
Seed germination vigor refers to the potential to accomplish rapid, precise germination and seedling formation from seeds in field conditions . Seed vigor has been shown to be closely related to crop field performance in variety of crops, especially in B. napus [18, 19, 20, 21]. After long-term storage, the seeds gradually deteriorate and lose their activity, which is known as the aging of seeds. High temperature and high humidity storage conditions could accelerate the aging process of seeds [9, 22]. This is an important problem in practical seed production, and a significant cost has been paid in order to reduce the loss of seed vigor during storage [23, 24]. Based on the present results in Arabidopsis, oxidative stress was an important factor in seed aging . The loss of seed vigor during aging could be attributed to two aspects: one was protein changes in dry seeds, and the second was that low-activity seeds cannot express normal proteomes during germination . The key mechanisms for maintaining seed vigor include the translation capacity, seed storage mobilization and antioxidant efficiency . Additionally, some late embryogenesis abundant (LEAs) proteins and HSPs also play an important role in maintaining seed vigor .
The fatty acid (FA) composition of rapeseed was changed dramatically in the aging process. In addition, the aging of seeds could also prolong the germination time, reduce the germination and the overwinter rate, and decrease the rapeseed yield [21, 26]. B. napus seeds are always sown in autumn and harvested in late spring, so the seeds will go through a hot and humid summer, which can lead to the loss of germination vigor. Therefore, a comprehensive understanding of the molecular mechanism of seed germination and germination vigor is important for reducing the loss of seed vigor in B. napus. Yin et al. demonstrated that the primary factor contributing to the aging of B. napus seeds might not be the accumulation of reactive oxygen species (ROS) but the accumulation of endogenous abscisic acid (ABA) during seed aging . Morris et al. have found that two genes, BoLCVIG1 and BoLCVIG2, and a QTL (Reduced ABscisic Acid 1, RABA1) were associated with the determination of seed vigor in Brassica oleracea, and these genes were associated with ABA metabolism or seed sensitivity to ABA . In addition, proteins that are involved in metabolic and protein transport were reduced by artificial aging treatment, whereas actin, mannose-binding lectin superfamily protein, glycosyltransferase, beta-glucosidase and other cell and cell wall structure-related protein content increased . Moreover, previous studies also have shown that the loss of seed vigor during storage in B. napus seeds with different oil content is distinct . Similarly, the loss of seed vigor in low oil-containing seeds was significantly lower than that of seeds with high oil content under the same conditions . These results indicate that the seed vigor of rapeseed might be correlated with oil content.
The advent of powerful statistical methods for linkage-based QTLs mapping combined with the available diversity of the germplasm and the completion of reference genome sequencing in B. napus, allowing the genetic architecture of complex traits to be uncovered in B. napus [29, 30, 31, 32]. The combination of QTL mapping and multi-omics studies provides a powerful framework for the functional characterization of genes . For instance, this strategy has been used to identify genes controlling lipid biosynthesis in maize . In this class of approaches, the candidate genes can be narrowed by utilizing multiple datasets . These results indicate that the identification of candidate genes could be easier and more reliable by combining ‘-omics’ methods and QTL mapping.
The decreased quality and germination vigor of B. napus seeds during long-term storage is a substantial problem. Therefore, improving seed germination vigor and weakening or eliminating the adverse effects of aging on seed germination vigor are related to the improvement of the yield and the quality of the domestic edible oil, which has great significance. This requires a detailed understanding of the regulation mechanism for seed germination and germination vigor in B. napus. In this paper, DEPs were observed in the seed germination process of rapeseed lines with high and low oil content, and important candidate genes were identified by combining the QTL analysis for controlling of seed germination vigor in B. napus. The results lay the foundation for the ultimate improvement of seed germination vigor and weakening or eliminating the adverse effects of aging on seed germination in B. napus.
Effects of oil content on seed germination vigor in B. napus
Major metabolite changes in germinating seeds with high and low oil content
To evaluate the mobilization of storage reserves in seeds during germination and post-germination, the total oil content was measured (Additional file 3), and it was revealed that the changing tendency of oil content in germinating KenC8 and 12WH191 seeds was similar, and significant changes only occurred during post-germination seedling growth (Additional file 3). This result suggests that the storage of lipids might be mobilized during germination and are mainly consumed during the post-germination stages. The difference in the relative content of FAs was dramatically observed in these two materials (Additional file 4). In dry seeds, the content of C18:3 in KenC8 was significantly lower than that in 12WH191; otherwise, the content of C18:1 and C18:2 in KenC8 were higher than that in 12WH19. Though some changes of C18:3, C22:1, eicosanoids (C20:0), eicosanoic acid (C20:1) and palmitic acid (C16:0) could be observed in Ken C8 and 12WH191 during germination, the FAs remained relatively stable during germination and post-germination growth.
The changes in protein and sugar content were observed during the germination process (Additional file 3). It was revealed that the total protein content of both KenC8 and 12WH191 increased rapidly after imbibition (0–12 HAI) and was followed by a gradual decrease. At 72 h, the total protein content of both had been reduced to approximately 30 mg/g. This result suggests that in the early stages of germination (0–12 h), there is a large amount of new protein synthesis in the germinating seed, and a large amount of stored protein is consumed as the source of energy and substance metabolism for seed germination afterwards. In the early stages of germination, the total sugar content in the seeds decreased gradually. The total sugar content of both cultivars increased rapidly following 36 HAI, suggesting that the sugars in the seeds might be the source of energy and substance metabolism required for early seed germination.
Other physiological characteristics that might reflect the physical and metabolic energy use during germination were also determined, including soluble sugar, pyruvate and acetyl-CoA (Additional file 3). There were no significant differences in the pyruvate content between KenC8 and 12WH191 during germination. In dry seeds, the soluble sugar content in 12WH191 was essentially equal to Ken C8. However, the content of soluble sugar in KenC8 increased rapidly during early germination (0–12 h) and then decreased gradually, and the soluble sugar content in 12WH191 was always lower than that in KenC8 during germination. The change of acetyl-CoA was nearly same in KenC8 and 12WH191, which gradually decreased in the early stage of germination, and then gradually increased, but began to decrease again after 36 HAI. The content of acetyl-CoA in KenC8 was always lower than that in 12WH191, especially at 0–24 HAI. The content of acetyl-CoA in 12WH191 was 2–3 times higher than that in KenC8. This might imply that the substance and metabolic energy usage in low oil-containing seeds was significantly lower than that in high oil-containing seeds.
As an important plant hormone, the content of ABA was also compared between 12WH191 and KenC8 during germination (Additional file 3). It was shown that the content of ABA decreased along with germination in both cultivars, but it was significant higher in KenC8 seeds with lower oil content. This result implies that ABA might participate in the determination of seed vigor in B. napus seeds with different oil content.
Microstructure comparison of embryonic cells during germination in seeds with high and low oil content
Comparative proteomic analysis in seeds with high and low oil content
Thirty-nine DEPs were involved in stress and defense, of which 22 spots were expressed significant higher in 12WH191 than in KenC8 in at least one germination stage. For example, compared to expression in KenC8, myrosinase-binding protein 2 (spot 59) was significantly higher in 12WH191 at 12 and 36 HAI (P < 0.05), while two other protein spots were higher in 12WH191 at 24 HAI (P < 0.05; Additional file 1: Table S3). Similarly, mannose-binding lectin superfamily protein (spot 522 and 1492), monodehydroascorbate reductase 1 (spot 1176), probable aldo-keto reductase 6 (spot 856) and dehydrin Rab18 (spot 1175) were highly expressed at one or more germination stages in germinating seeds of 12WH191 (P < 0.05; Additional file 1: Table S3). In contrast, other DEPs showed higher expression in germinating seeds of KenC8 compared to expression in 12WH191 seeds, including myrosinase-binding protein 2 (spot 51, 53 and 120), peptide methionine sulfoxide reductase B5 (spot 1668), epithiospecifier protein (spot 753 and 754), iron/manganese superoxide dismutase family protein (spot 1207), superoxide dismutase [Mn] (spot 1217), thioredoxin-dependent peroxidase 1 (spot 1319) and 1-cys peroxiredoxin PER1 (spot 1472) (P < 0.05; Additional file 1: Table S3). These results indicated that different stress- and defense-related proteins were activated or inhibited in response to internal and external stress and stimulation during seed germination, and that there might be significant differences in the occurrence and establishment of defense systems in the process of germination.
Thirty-two DEPs were involved in protein synthesis, folding, transport, modification and degradation, of which 21 protein spots were highly expressed in 12WH191 (Additional file 1: Table S3). For example, aspartic proteinase A1 (spot 980, 992 and 1655), protein disulfide isomerase-like 1–2 (spot 428, 432 and 436), elongation factor EF-2-like protein LOS1 (spot 97), heat shock cognate protein 70–1 (spot 292), heat shock protein 81–2 (spot 211), heat shock protein 70–4 (spot 286), luminal-binding protein 2 (spot 267), protein phosphatase 2A subunit A2 (spot 332), chaperonin-60 alpha (spot 407), TCP-1/cpn60 chaperonin family protein (spot 411), translational initiation factor 4A-1 (spot 633), etc., (Additional file 1:Table S3) were all highly expressed in 12WH191. In addition, 24 and 12 DEPs that are related to energy metabolism as well as sugar and carbohydrate metabolism involving tricarboxylic acid (TCA) cycling, glycolysis, gluconeogenesis, adenosine triphosphate (ATP) synthesis, glycerol and galactose metabolism were identified. In addition, most of these DEPs were also expressed significantly higher in 12WH191 compared to expression in KenC8 (P < 0.05), such as malate dehydrogenase and malate dehydrogenase 1 (spot 808, 816, 892 and 1094) and dihydrolipoyl dehydrogenase 1 (spot 438), which were highly expressed in high oil seedlings at several stages of germination (Additional file 1: Tables S3 and S5). It was shown that energy metabolism and carbohydrate metabolic activity might be significantly higher in 12WH191 than in KenC8, especially in the late stage of germination. In addition, there were 13 DEPs involved in amino acid metabolism, such as glutathione S-transferase (spot 1202, 1204, 1467 and 1709) and mercaptopyruvate sulfurtransferase 1 (spot 867), which were highly expressed in germinated 12WH191seeds.
Interestingly, we found that five DEPs (spot 125, 153, 370, 606 and 1108) were involved in signal response and conduction, and besides thioglucoside glucohydrolase 1 (spot 370), other DEPs were significantly higher in germinated seeds of 12WH191 than of KenC8 and reached their peak at 36 or 48 HAI (Additional file 1: Tables S3 and S5). Mature proteins in seeds and LEAs were also been identified; these proteins are involved in seed maturation and were highly expressed during seed development and maturation. The present results demonstrated that the expression level of these proteins was mostly reduced during seed germination, but the expression level of two LEAs (spot 192 and 506, late embryogenesis abundant protein) fluctuated during germination and post-germination seedling growth and were highly expressed after 36 HAI.
Expression pattern analysis of the DEPs
Interaction analysis of DEPs and comparison of metabolic pathways in germinating seeds with high and low oil content
QTL mapping and identification of important candidate genes that correspond to the DEPs
The DEPs were first screened as previously reported in the germinating B. napus seed . Then, the genes corresponding to the DEPs that were obtained by proteomic analysis were used for alignment with the QTL intervals for GI and GP to verify whether the DEPs between the HO and LO cultivars were underlying the variation in seed germination vigor. The results revealed 31 unique genes that were also observed previously, and 13 genes of which were within the confidence intervals (CIs) of QTLs for GI and/or GP that were detected in the KNDH population (Additional file 1: Tables S7 and S8). BnaC01g43710D (AT3G21380, Mannose-binding lectin superfamily protein) was located in the CI of q13GI-C1–1. BnaC06g19960D (AT1G79230, mercapto-pyruvate sulfurtransferase 1), BnaC06g20420D (AT1G78380, glutathione S-transferase TAU 19), BnaA07g20290D (AT1G79550, phosphoglycerate kinase) and BnaA07g20210D (AT1G79690, nudix hydrolase 3) were located within the CI of q13GI-C6–2. BnaA06g16950D, which is homologous to AT3G48000 and encodes aldehyde dehydrogenase 2B4, was identified underlying q13GP-A3 and q13GI-A3. BnaC04g42010D (AT5G02500, heat shock cognate protein 70–1) was identified as underlying q13GP-C5 and q13GI-C5–4. BnaC06g09330D (AT1G52030, myrosinase-binding protein 2) and BnaC03g70070D (AT3G13920, translational initiation factor 4A-1) were identified in the CI of the major QTL, q13GP-C6–1. BnaA07g33310D (AT1G77120, alcohol dehydrogenase 1) and BnaC06g38200D (AT1G77510, protein disulfide isomerase-like 1–2) colocalized within q13GP-C6–2 and q13GI-C6–2. BnaC06g14990D (AT3G53990, Adenine nucleotide alpha hydrolases-like superfamily protein) and BnaC06g14670D (AT1G48130, 1-Cys peroxiredoxin PER1) were located in the CI of q13GI-C6–1 and q13GP-C6–1 (Fig. 9; Additional file 1: Tables S7 and S8). The 13 genes that corresponded to the DEPs were identified within QTL CIs for GI and/or GP, indicating that these DEPs might be more related to seed germination vigor.
Oil content affects seed germination in B. napus
Seed vigor is the ability of seeds to quickly and precisely germinate and successfully build healthy seedlings, which is an important factor in determining crop yields [18, 20]. Seed vigor decreases with seed aging [18, 19, 20]. Some studies have shown that the seed vigor of B. napus is affected by artificial or natural aging treatments for seeds with different oil contents [9, 26]. The present results indicated that the average germination index for high oil content cultivars was slightly higher than that of cultivars with low oil content. The germination rate, germination initiation time and germination index of some high oil-containing cultivars were significantly higher than these measures in the low oil-containing cultivars. This result indicates that the seed germination vigor of B. napus might be affected by oil content.
Abscisic acid and its signaling pathways may be related to the differences in seed vigor in B. napus with high and low oil content
ABA has been recognized as an important factor in determining plant seed germination . During the transition from dormancy to germination, the susceptibility of seeds to ABA and indole-3-acetic acid (IAA) decreased . A transcriptional analysis of the wheat seed response to ABA demonstrated that ABA inhibited the germination of wheat seeds by inhibiting chromosome assembly, cell wall modification, and the expression of gibberellin (GA)-metabolic activation-related genes [2, 36]. Morris et al. found that two genes (BoLCVIG1 and BoLCVIG2) and a QTL (Reduced ABscisic Acid 1, RABA1) were associated with seed vigor in Brassica oleracea, which were demonstrated to be associated with ABA metabolism or the ABA sensitivity of seeds . The germination rate of B. oleracea seeds might be determined by the combination of seed ABA content and seed ABA sensitivity . Germination is regulated by the increasing growth potential of the radicle and the resistance of the testa and endosperm tissues covering the radicle . Embryo elongation growth requires cell expansion in defined regions of the radicle and the lower hypocotyl, which is promoted by GA and inhibited by ABA [2, 38]. The resistance of the surrounding tissues could be reduced by enzymatic or physical weakening of their cell walls. Further, in Lepidium sativum and Arabidopsis, it has been shown that ABA delays endosperm rupture . The liquid chromatograph mass spectrometer (LC-MS) analysis indicated that the content of ABA was higher in KenC8 during early germination (0–24 HAI). The endosperm rupture and the cell expansion in the radicle and lower hypocotyl may be delayed by the higher ABA content in B. napus seeds with low oil content. In addition, the present results showed that three DEPs, thioglucoside glucohydrolase 1 (spot 370) and protein low-temperature-induced 65 (spot 125 and 153), that associated with ABA-activated signaling pathways differed significantly between 12WH191 and KenC8 during germination. The protein low-temperature-induced 65 was highly expressed in 12WH191 throughout the germination process; otherwise, the expression of thioglucoside glucohydrolase 1 was significantly higher in the KenC8 initially and then it was more highly expressed in 12WH191 during the subsequent germination process. Although there are no reports on the role of thioglucoside glucohydrolase 1 in seed germination, previous studies have shown that it might be involved in ABA-related signaling pathways in leaf-guarding cells . The protein low-temperature-induced 65 is induced by low temperature, drought, etc., and this induction is associated with ABA . Therefore, the difference in seed vigor in B. napus cultivars may be related to seed ABA content and the ABA-related signaling pathways.
Defense systems may contribute to the high germination vigor of seeds with high oil content
Seeds and seedlings are very sensitive to stress . Therefore, the establishment of a defense system during seed germination may play an important role in ensuring normal seed germination. Many studies have emphasized the importance of cell defense mechanisms in the establishment and regulation of seed germination [43, 44, 45]. For example, low concentrations of salicylic acid (SA) can enhance the expression of some superoxide dismutase under salt stress. In addition, SA also enhances the re-induction of the late-maturing process in the early stages of germination, the glyoxylate cycle and methionine metabolism, as well as the mobilization of seed storage proteins. All of these SA-affected responses may help to improve the suitability of seeds for the environment and improve seed vigor . The sulfur-containing amino acid metabolic pathway and the myrosinase-glucosinolate system play an important role in plant development and defense . The sulfur amino acid metabolic pathway is a key determinant of whether the seed can initiate germination2, and inhibition of sulfur-containing amino acid metabolism can strongly delay seed germination and seedling growth [47, 48, 49]. Methionine is not merely a structural unit for protein synthesis; it also participates in the synthesis of S-adenosylmethionine and thus affects the synthesis of polyamines, ethylene and the vitamin biotin . Glutathione and glutathione disulfide are essential for the reduction of various peroxides [51, 52]. Glutathione S-transferase plays a direct role in the response to external environmental stimuli, reduced oxidative damage, and damage from foreign substances [53, 54]. The present results revealed that 7 sulfur amino acid metabolism-related DEPs were highly expressed in high oil-containing seeds, and two corresponding genes (AT1G79230 and AT1G78380) located within the CIs of QTLs for GP and GI were also identified. This suggests that higher sulfur-containing amino acid metabolic activity might help to improve seed suitability for the environment and seed vigor. The DEPs that correlated with the myrosinase-glucosinolate system were also identified in the germinating high and low oil-containing seeds; the myrosinase-binding protein 2 gene corresponding to the DEPs were located within the QTLs for GI and GP. There were three myrosinase-binding protein 2 spots (spot 59, 1516 and 1588) that were highly expressed in the seeds with high oil content, but the other three (spot 51, 53 and 120) were highly expressed in the seeds with low oil content, which indicated a complex relationship between the myrosinase-glucosinolate system and germination vigor.
Seeds with higher oil content have more active substance and energy metabolism during germination
LEA and HSPs play an important role in maintaining seed vigor . It was revealed that maize seeds with high vigor synthesized more heat shock proteins during germination, and five HSPs with molecular weights of 73 kD, 65 kD, 62 kD, 54 kD and 18 kD were shown to differ quantitatively between high and low vigor maize seeds. A special HSP with a molecular weight of 56 kD was only present in seeds with high vigor; thus, it might be used as an indicator of seed vigor . Similarly, the heterogenously expressed HSP gene of lotus (NnHSP17.5; Nelumbo nucifera Gaertn.) in the A. thaliana could significantly enhance the germination potential of transgenic Arabidopsis seeds and improved the thermal tolerance of seedlings . Consistent with this result, the high expression of LEA (spot 192, 506, and 528) proteins and HSPs (spot 211, 286 and 292) was identified in the proteomic experiments in the seeds with high oil content and was also detected in the CIs of the QTLs for GP and GI. Moreover, many important proteins related to protein synthesis, folding and modification were also highly expressed in the high oil-containing seeds, such as protein disulfide isomerase (spot 428, 432 and 436), chaperonin-60 (spot407 and 411), transcriptional initiation factor 4A-1 (spot 630) and tRNA synthetase (spot 477 and 1576). AT1G77510 and AT3G13920 encode protein disulfide isomerase and transcriptional initiation factor 4A-1, respectively, and were identified as located within the CIs of the QTLs for GP and GI. These DEPs might play an important role in ensuring the normal and rapid expression of the proteome during germination [57, 58, 59]. Protein synthesis is an absolute requirement for seed germination because the inhibition of protein translation can induce a complete block of Arabidopsis seed germination, and an apparent correlation between the protein translational ability and seed vigor has been identified [25, 60, 61]. A more active protein metabolism may be correlated with high seed vigor in B. napus seeds with high oil content.
In addition, the mobilization of seed storage reserves is one of the key mechanisms for maintaining seed vigor . The glycolytic pathway is necessary for the mobilization of storage reserves during seed germination and is the main source of energy and material required for seed germination and seedling growth. Therefore, the glycolysis process is closely related to the rate of seed germination and seedling formation [2, 6, 62]. In the artificial aging of B. napus seeds, some metabolic-related proteins are inhibited by aging, which is thought to be associated with the reduction of seed germination vigor . By comparing the differences in proteomics during the germination of high and low oil-containing seeds, we found that 10 amino acid metabolism-related proteins, 11 sugar and carbohydrate metabolism-related proteins, 15 proteins correlated with glycolysis/gluconeogenesis, TCA cycle and pyruvate metabolism and other energy metabolism processes were highly expressed in the high oil-containing seeds. Some genes corresponding to these DEPs were found to be located in the QTL confidence intervals for GI and GP by the QTL alignment analysis, such as AT1G79550, AT1G77120 and AT3G48000, which encode phosphoglycerate kinase, alcohol dehydrogenase 1 and aldehyde dehydrogenase 2B4, respectively. Surprisingly, the total oil content in the dry matter increased significantly at 24 HAI in seeds with both high and low oil content and peaked at 48 and 36 HAI, respectively. A similar result was also observed in germinating jatropha seeds, but no relevant research focused on the mechanism underlying this phenomenon . Embryo elongation growth requires cell expansion in defined regions of the radicle and lower hypocotyl during early seed germination, while cell division mainly occurs during post-germination seedling growth . Large amounts of cell proliferation require the accumulation of phospholipids and may cause lipid metabolism to shift toward lipid regeneration. This requirement may be why the total oil content in dry matter increased significantly at 24 HAI. This increase in oil content was significantly higher in B. napus seeds with high oil content, suggesting that cell proliferation may be more exuberant in B. napus seeds with high oil content, which was consistent with seedling growth. These results showed that high oil-containing seeds have higher substance and energy metabolic activity during germination, which can accelerate storage substance mobilization, which provides the synthetic material and energy required for seed germination and seedling growth.
Among the candidate genes we found were involved in the regulation of high and low oil seed germination vigor by the methods of QTL mapping and proteomics, only one gene (AT5G02500) was annotated as seed germination in the NCBI database. We speculated that there are two main reasons for this issue, (i) some DEPs have not been successfully detected, (ii) the difference in germination vigor between high and low oil content materials may not be attributed to the main genes involved in seed germination as reported previously. This might suggest that the regulatory mechanisms for germination vigor between high and low oil content B. napus seeds are more complex than we predicted. The present results not only emphasize the important role of protein synthesis, folding and degradation processes and the glycolysis process in the regulation of seed germination vigor but also suggest that sulfur amino acid metabolism and defense systems are also involved in the regulation of seed germination vigor. Some candidate genes are identified and further investigation of the functional analysis of these genes is needed in the future.
Twenty B. napus cultivars with different oil content were used (Additional file 1: Table S9). All the materials were stored in laboratory of Pro. Li Maoteng of huazhong university of science and technology. The seeds were imbibed in ultrapure water at 28 °C in the dark. The seeds/seedlings were collected for physiological indices, gene expression and proteomics analysis at 0, 12, 24, 36, 48, 60 and 72 HAI. Tissues were stored at − 80 °C until analysis.
Seed germination index and transmission electron microscopy analysis
The number of newly germinated seeds was counted every 4 h, and then the seed germination index was calculated according to the described method .
Transmission electron microscopy (TEM) analysis was carried out according to the method described by Gan et al. . Cotyledons of dry seeds and germinated seeds were collected and fixed with 2.5% glutaraldehyde for more than 24 h and then fixed with 1% osmium solution for 2 h. Next, the samples were dehydrated with gradient acetone (20, 50, 70, 90 and 100% × 3). Subsequently, the samples were infiltrated for 3 h in an acetone/epoxy resin mixture (acetone: epoxy, 2: 1) and epoxy resin and then infiltrated overnight using epoxy resin. The samples were finally embedded with epoxy resin and polymerized at 60 °C for 24 h. The samples were cut into 70 nm slices using a slicer (Leica MZ6, Germany), collected into a copper mesh and then stained with saturated uranium acetate and 0.4% lead citrate. The samples were rinsed with ultra-pure water 6 times, spaced with 15 s each time. Then, the sections were observed and imaged by a transmission electron microscope (JEM-1230, Japan).
Oil content and fatty acid composition analysis
The change in oil content during seed germination and post-germination growth was detected following an improved Soxhlet extraction method . Each sample was processed in three replicates. FA compositions were determined according to Browse et al. , using gas chromatography (GC-2010 Agilent, DEGS-diethyl glycol succinate column) with a flame-ionization detector. Relative FA compositions were calculated from five independent biological replicates.
Total protein and sugar content analysis in germinating seeds
The Bradford method was used for total protein quantitation . A standard solution (1 mg/ml γ-globulin) was used for the calibration curve, and 100 μl distilled water was used as the blank. Assay reagent (5 ml) was added into each tube and was well mixed by inversion or gentle vortex. The absorbance was measured at 595 nm. Protein samples (100 μl) were placed in centrifuge tubes and treated as described above. The total protein content of samples was calculated according to the standard curve.
Total sugar was analyzed with an improved method described by Wen et al. . A glucose standard solution (100 μg/ml) was used for the calibration curve, and the standard curve was obtained by using the glucose content as the abscissa and the absorbance value as the vertical axis. The absorbance of the samples and the standard solution was analyzed at 620 nm. Then, the total sugar content of the sample was calculated according to the standard curve.
Soluble sugar, pyruvate, acetyl-CoA and ABA analysis in germinating seeds
Soluble sugar was extracted with 1 ml deionized water in a boiling water bath for 10 min. The extracting solution was filtered into a 10 ml volumetric flask, and the volume was brought to 10 ml with deionized water . Detection was performed according to the above method for three replicates.
The change of pyruvate content during B. napus seed germination was determined by the 2,4-dinitrophenylhydrazine method with a pyruvate content determination kit (Comin Biotechnology; CHN) . Each sample was processed in three replicates.
The extraction and detection of acetyl-CoA was completed following a previously described method . The final volume of the standards was 50 μl, and the concentrations of the standards were 60 pmol/L, 40 pmol/L, 20 pmol/L, 10 pmol/L and 5 pmol/L. Each concentration gradient was repeated twice. Ultrapure water (50 μl) was used as the blank control. The sample diluent (40 μl) was added to 10 μl of sample for testing. Absorbance was measured at 450 nm. The acetyl-CoA content was calculated according to the standard curve.
ABA was extracted from germinating seeds according to previous descriptions . Then, ABA content was measured by following a derivatization approach as described previously, coupled with an API 4500 QTRAP LC/MS/MS system with a C18 column (WatersACQUITY UPLC HSS T3, 1.8 μm, 2.1 mm*100 mm) .
Protein extraction and fluorescent dye labeling
The seeds/seedlings of 12WH191 and Ken C8 were collected for proteomics analysis at 0, 12, 24, 36 and 48 HAI. Protein was extracted according to the method described previously [34, 65]. Approximately 0.5 g of seeds/seedlings was homogenized to fine powder in liquid nitrogen and then transferred 0.1 g sample into a 2 ml Eppendorf tube with three replicates. Each sample was homogenized in 750 μl Tris saturated phenol (pH > 7.8) and 750 μl of homogenization buffer containing 0.1 M Tris-HCl (pH 7.5), 0.9 M sucrose, 10 mM ethylene diamine tetraacetic acid (EDTA), 0.4% (g/ml) dithiothreitol (DTT) in an ice bath for 30 min. The homogenate was centrifuged at 5000 g for 15 min at 4 °C. The supernatant was transferred to a new tube and mixed with 1.5 ml 0.1 M ammonium acetate-methanol solution in − 20 °C for overnight. The mixture was centrifuged at 5000 g for 10 min at 4 °C, and then the precipitation was washed twice with 0.1 M ammonium acetate-methanol solution and twice with acetone. The extracted protein was dried under room temperature for about 5 min and dissolved in a buffer solution containing 7 M urea, 2 M thiourea, and 4% 3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) (pH = 8.8) and then was frozen at − 20 °C for experiments. The protein concentration in the sample was tested using the 2D Quant kit (GE Healthcare, UK) kit according to the instructions. The concentration of all protein samples was adjusted to 5 μg/μl before it was labeled with DIGE fluorescent dyes . For protein labeling, 50 pmol of CyDye(Cy2, Cy3 and Cy5) were mixed with 6 μl of protein sample and incubated on ice for at least 2 h in the dark. The labeling reaction was terminated by adding 1 μl of 10 mM lysine . Three replicates of each sample were labeled with Cy3/Cy5 dyes as Additional file 1: Table S10. The internal reference were labeled with Cy2 by equilibrating all protein samples of equal volume . Finally, mixes of the Cy2-, Cy3-, or Cy5-labeled protein were used for 2D-DIGE analysis.
Gel electrophoresis, image scan, and data analysis
For 2D-DIGE analysis, the labeled proteins were mixed with 2D-DIGE buffer (7 M urea, 2 M thiourea, 4% CHAPS, 0.4% DTT, 0.5% IPG buffer) and separated with isoelectric focusing after being loaded on an immobilized pH gradient strip (IPG, pH 4–7, 24 cm; Amersham Biosciences, Uppsala, Sweden) . The experimental conditions were followed as described by Gan et al. . The IPG strips were rehydrated for 14 h, followed by holding at 100 V, 300 V, 600 V, 1000 V for 1 h at each step, then the voltage was raised to 10,000 V at linear mode and held until reaching a total value of 120,000 V-h. Then the IPG strips were equilibrated for 15 min each in an equilibration buffer [6 M urea, 0.375 M Tris−HCl (pH 8.8), 20% glycerol, and 2% DTT] containing 2% sodium dodecyl sulfate (SDS) and 2.5% iodacetamide, respectively. The Second dimension electrophoresis was performed on 12.5% SDS-polyacrylamide gel with an Ettan DALT six electrophoresis system (GE Healthcare, UK). The electrophoresis was performed at 50 V for 2 h and then at 110 V until the bromphenol blue front arrived to the bottom of the gel. A Typhoon 8600 scanner (GE Healthcare, UK) was used for image collection and these images were then analyzed by DeCyder 6.5 software (GE Healthcare, UK) according to Gu et al.  Spot detection was performed by the differential in-gel analysis (DIA) module by setting the estimated spots number as 3500. After removing the artifact spots by manual editing, the images were further analyzed by the biological variation analysis (BVA) module. Three biological replicates were used for statistical analysis of each treatment. Spots showing a ≥ 1.2-fold change in spot volume were considered as differentially expressed spots (T-Test, P < 0.05).
Protein spot picking and identification
For spot picking, 1 mg protein samples were loaded onto IPG strips for isoelectric focusing, and then they were separated by 2D electrophoresis as described in the method above . Gels were stained with coomassie brilliant bule R-250 (CBB R-250) and scanned by a UMAX Power Look 2100XL scanner (UMAX, Inc., Taipei, China) . Then, the DEPs were selected by matching the spot-picking gel map and DIGE map, and then were manually excised from the gels for mass spectrometry (MS) and MS/MS analysis [65, 74, 75, 76].
The gel pieces were distained with 50 mM NH4HCO3 in 50% (v/v) methanol for 1 h at 40 °C twice. Then the protein in the gel piece was reduced with 10 mM EDTA, 10 mM DTT in 100 mM NH4HCO3 for 1 h at 60 °C and incubated with 10 mM EDTA, 40 mM iodoacetamide in 100 mM NH4HCO3 for 30 min at room temperature under the dark. The gel pieces were minced and lyophilized, then rehydrated in 25 mM NH4HCO3 with 10 ng of sequenced grade modified trypsin (Promega, Madison, WI) at 37 °C overnight. After digestion, the protein peptides were collected, and the gels were washed twice with 0.1% trifluoroacetic acid (TFA) in 50% acetonitrile to collect the remaining peptides. The peptides were desalted by ZipTipC 18 pipet tips (Millipore, Bedford, MA) and co-crystallized with 1 volume of saturated R-cyano-4-hydroxycinnamic acid in 50% (v/v) acetonitrile containing 1% TFA.
The desalted protein samples were subject to ABI 5800 MALDI-TOF/TOF Plus mass spectrometer (Applied Biosystems, USA), using a surveyor high performance liquid chromatography (HPLC) system. Data were acquired in a positive MS reflector using a CalMix5 standard to calibrate the instrument (ABI5800 Calibration Mixture). The mass spectrometry range of peptide mass fingerprinting (PMF) was set to 800–3500 Da and followed by 10 MS/MS scans on the 10 most intense ions from the MS spectrum. Both the MS and MS/MS data were integrated and processed by using the GPS Explorer V3.6 software (Applied Biosystems, USA) with default parameters. Based on combined MS and MS/MS spectra, proteins were successfully identified based on 95% or higher confidence interval of their scores in the MASCOT V2.3 search engine (Matrix Science Ltd., London, U.K.), using the following search parameters: NCBInr-Brassica napus database; trypsin as the digestion enzyme; one missed cleavage site; fixed modifications of Carbamidomethyl (C); partial modifications of Acetyl (Protein N-term), Deamidated (NQ), Dioxidation (W), Oxidation (M); 100 ppm for precursor ion tolerance and 0.5 Da for fragment ion Tolerance .
Protein classification, hierarchical clustering analysis and interaction analysis of DEPs
DEPs were classified into different categories using NCBI (https://www.ncbi.nlm.nih.gov/), TAIR (http://www.arabidopsis.org/index.jsp) and KEGG (http://www.genome.jp/kegg/) databases. To visualize the expression characteristics of the DEPs, a hierarchical cluster was constructed using the K-means clustering approach in SPSS .
Interactions between DEPs were analyzed by STRING 10.0 (http://string-db.org). The locus tag of the Arabidopsis homologous corresponding to the DEPs and DEGs were used as inputs for STRING 10.0 to create the interaction network. The main interaction analysis parameters were as follows: The organism was set to A. thaliana; the interaction analysis was based on the default selection for all options, including text mining, experiment, database, coexpression, neighborhood, gene fusion and simultaneous occurrence; the interaction score was set to medium confidence (i.e., the interaction score is 0.4); the remaining parameters were selected as the default. In addition, the network was then displayed by Cytoscape 3.2.
QTL mapping of seed germination index and germination potential
The high-density linkage genetic map used for QTL mapping of the seed germination index and germination potential was constructed based on the segregating double haploid (DH) population, named the KNDH population by Chao et al. . The KNDH population was derived from a cross between the parental lines KenC-8 with low oil content and N53–2 with high oil content. The seeds of the KNDH population that were used for the germination index and germination potential analysis were collected from Wuhan of Hubei Province (a semi winter-type rapeseed growing area, coded WH) and Dali of Shannxi Province (a winter-type rapeseed growing area, coded DL) in 2014. The QTL analysis for the seed germination index and seed germination potential was performed as described by Chao et al. . Putative QTLs was detected by Windows QTL Cartographer 2.5 under the CIM model. The scan walking speed was 2 cM, and the window size was 10 cM with five background cofactors. The LOD threshold (< 2.0) for the detection of QTLs was calculated by a 1000-permutation test based upon a 5% experiment-wise error rate. All of these QTLs were named by combining the microenvironment and the chromosome number, such as q13GI-C1–2. Gene alignment and candidate gene identification within QTL regions were performed according to the method that was described by Chao et al. .
Gene expression analysis of candidate genes
An RNAprep Pure plant kit (TOYOBO, Japan) was used for the extraction of total ribonucleic acid (RNA). According to the user manual, the first chain was synthesized using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO, Japan). The ABI 7900HT Fast Real-Time PCR System (Applied Biosystems, USA) was used for gene expression analysis with the SYBR premix EX TaqTM kit (TaKaRa, Japan). Relative gene expression was calculated according to Pfaffl’s method . Each sample was completed in three replicates for calculating standard deviation (SD) errors to evaluate the intra-assay variation. BnaActin 2.1 was used as the reference gene . The primers that were used in this study are listed in Additional file 1: Table S11.
This work was financially supported by the National Science Foundation of China (31471532), the National Basic Research Program of China (2015CB150205), the New Century Talents Support Program by the Ministry of Education of China (NCET110172), the Hubei Provincial Natural Science Fund Knowledge Innovation Project (2018CFC851) and the Hubei Provincial Department of Education Science and Technology Research Program Youth Talent Project (Q20182707). The funder had no role in the study design, data collection and analysis or preparation of the manuscript.
Availability of data and materials
The datasets generated and analyzed during the current study are available from the corresponding author and first author on reasonable request.
All authors have read and approved the manuscript. ML, JX and BW conceived and designed the study. YL and HW collected the plant samples. JG and DH performed the experiments and analyzed the data. HC, KZ and NR analyzed the data. JG and DH contributed equally to this paper.
Ethics approval and consent to participate
The seed of B. napus were from the Hybrid Rapeseed Research Center of Shaanxi Province in Shaanxi Province.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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