Advertisement

BMC Molecular Biology

, 20:20 | Cite as

Correction to: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone

  • Neftali Vazquez
  • Lilia Sanchez
  • Rebecca Marks
  • Eduardo Martinez
  • Victor Fanniel
  • Alma Lopez
  • Andrea Salinas
  • Itzel Flores
  • Jesse Hirschmann
  • Robert Gilkerson
  • Erin Schuenzel
  • Robert Dearth
  • Reginald Halaby
  • Wendy Innis-Whitehouse
  • Megan KeniryEmail author
Open Access
Correction
  • 43 Downloads

Correction to: BMC Molecular Biology (2018) 19:3  https://doi.org/10.1186/s12867-018-0105-8

The original article [1] contains three erroneous mentions of usage of a restriction enzyme—BstZ17I—in the Methods section as displayed in the following sentences:
  1. 1.

    […] Therefore, FOXO3 gene fragments (PCR products) had pcDNA3 sequences on the ends that corresponded to upstream and downstream sequences of the utilized restriction sites (DraIII for Arm1 and BstZ17I for Arm2) in pcDNA3.

     
  2. 2.

    […] The intermediate vector (with FOXO3 Arm 1) was cut with BstZ17I, which is on the other side of the neomycin resistance gene in the pcDNA3 plasmid compared to FOXO3 Arm 1.

     
  3. 3.

    […] FOXO3 Arm 2 was amplified with the primer pair specified in Table 1, producing a product that had sequences on each end that were identical to the sequences proximal to the BstZ17I site in the intermediate FOXO3 Arm 1 vector.

     

As such, the above three sentences should instead have stated the correct restriction enzyme—BsmI—in place of where BstZ17I was mentioned in each instance.

Notes

Reference

  1. 1.
    Vazquez N, Sanchez L, Marks R, Martinez E, Fanniel V, Lopez A, Salinas A, Flores I, Hirschmann J, Gilkerson R, Schuenzel E, Dearth R, Halaby R, Innis-Whitehouse W, Keniry M. A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone. BMC Mol Biol. 2018;19:3.  https://doi.org/10.1186/s12867-018-0105-8.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Neftali Vazquez
    • 1
  • Lilia Sanchez
    • 1
  • Rebecca Marks
    • 1
  • Eduardo Martinez
    • 1
  • Victor Fanniel
    • 1
  • Alma Lopez
    • 1
  • Andrea Salinas
    • 1
  • Itzel Flores
    • 1
  • Jesse Hirschmann
    • 1
  • Robert Gilkerson
    • 1
  • Erin Schuenzel
    • 1
  • Robert Dearth
    • 1
  • Reginald Halaby
    • 2
  • Wendy Innis-Whitehouse
    • 3
  • Megan Keniry
    • 1
    Email author
  1. 1.Department of BiologyUniversity of Texas-Rio Grande ValleyEdinburgUSA
  2. 2.Department of BiologyMontclair State UniversityMontclairUSA
  3. 3.School of MedicineUniversity of Texas-Rio Grande ValleyEdinburgUSA

Personalised recommendations