Correction to: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone
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Correction to: BMC Molecular Biology (2018) 19:3 https://doi.org/10.1186/s12867-018-0105-8
[…] Therefore, FOXO3 gene fragments (PCR products) had pcDNA3 sequences on the ends that corresponded to upstream and downstream sequences of the utilized restriction sites (DraIII for Arm1 and BstZ17I for Arm2) in pcDNA3.
[…] The intermediate vector (with FOXO3 Arm 1) was cut with BstZ17I, which is on the other side of the neomycin resistance gene in the pcDNA3 plasmid compared to FOXO3 Arm 1.
[…] FOXO3 Arm 2 was amplified with the primer pair specified in Table 1, producing a product that had sequences on each end that were identical to the sequences proximal to the BstZ17I site in the intermediate FOXO3 Arm 1 vector.
As such, the above three sentences should instead have stated the correct restriction enzyme—BsmI—in place of where BstZ17I was mentioned in each instance.
- 1.Vazquez N, Sanchez L, Marks R, Martinez E, Fanniel V, Lopez A, Salinas A, Flores I, Hirschmann J, Gilkerson R, Schuenzel E, Dearth R, Halaby R, Innis-Whitehouse W, Keniry M. A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone. BMC Mol Biol. 2018;19:3. https://doi.org/10.1186/s12867-018-0105-8.CrossRefPubMedPubMedCentralGoogle Scholar
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