Functional analysis of Leifsonia xyli subsp. xyli membrane protein gene Lxx18460 (anti-sigma K)
Sugarcane is an important sugar and economic crop in the world. Ratoon stunting Disease (RSD) of sugarcane, caused by Leifsonia xyli subsp. xyli, is widespread in countries and regions where sugarcane is grown and also limited to sugarcane productivity. Although the whole genome sequencing of Leifsonia xyli subsp. xyli was completed, progress in understanding the molecular mechanism of the disease has been slow because it is difficult to grow in culture.
The Leifsonia xyli subsp. xyli membrane protein gene Lxx18460 (anti-sigma K) was cloned from the Lxx-infected sugarcane cultivar GT11 at the mature stage using RT-PCR technique, and the gene structure and expression in infected sugarcane were analyzed. The Lxx18460 gene was transformed into Nicotiana tabacum by Agrobacterium tumefaciens-mediation. The transgenic tobacco plants overexpressing Lxx18460 had lower levels in plant height, leaf area, net photosynthetic rate and endogenous hormones of IAA, ABA and GA3, as well as lower activities of three antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than the wild type (WT) tobacco. With the plant growth, the expression of Lxx18460 gene and protein was increased. To better understand the regulation of Lxx18460 expression, transcriptome analysis of leaves from transgenic and wild type tobacco was performed. A total of 60,222 all-unigenes were obtained through BGISEQ-500 sequencing. Compared the transgenic plants with the WT plants, 11,696 upregulated and 5949 downregulated genes were identified. These differentially expressed genes involved in many metabolic pathways including signal transduction, biosynthesis of other secondary metabolism, carbohydrate metabolism and so on. Though the data presented here are from a heterologous system, Lxx 18460 has an adverse impact on the growth of tobacco; it reduces the photosynthesis of tobacco, destroys the activity of defense enzymes, and affects the levels of endogenous hormones, which indicate that Lxx18460 may act important roles in the course of infection in sugarcane.
This is the first study on analyzing the function of the membrane protein gene Lxx18460 of anti-sigma K (σK) factor in Leifsonia xyli subsp. xyli. Our findings will improve the understanding of the interaction between the RSD pathogen Leifsonia xyli subsp. xyli and sugarcane. The output of this study will also be helpful to explore the pathogenesis of RSD.
KeywordsRatoon stunting disease Lxx18460 Transgenic tobacco Transcriptome Functional analysis
Fragments per kilobase of transcript per million mapped reads
Leifsonia xyli subsp. Xyli
National Center for Biotechnology Information
Open reading frame
Rapid amplification of cDNA ends
Reactive oxygen species
Ratoon stunt disease
Reverse transcription-polymerase chain reaction
Sodium dodecyl sulfate
Sugarcane is an important economic crop in Guangxi province which contributes more than 60% of the total sugar production in China [1, 2]. Ratoon stunting disease (RSD), caused by a nutritionally fastidious Gram-positive bacterium (Leifsonia xyli subsp. xyli – Lxx), is widespread in worldwide sugarcane production areas [3, 4] and responsible for substantial losses in yield [5, 6, 7, 8]. RSD control measures include meristem tissue culture, sterilization of harvesting and planting equipment and seedcane heat treatment. However, all of these measures have limited efficacy, RSD persists in sugarcane as a major threat to sugar industry.
Lxx colonizes at xylem vessels, mesophyll and bundle sheath cells surrounding the vascular system [9, 10]. Lxx is genetically uniform, and it has no genetic variation [7, 11]. The whole genome sequencing of Lxx (CTCB07) was completed by Monteiro-Vitorello et al. . The sequencing results have caused research interests in host recognition, pathogenicity gene and infection mechanism. Two possible pathogenicity genes, that is, pectinase and cellulose genes would be involved in cell wall degradation, but no evidence of this in the electron micrographs has been shown in the literature [13, 14]. Other pathogenicity genes may be involved in the production of abscisic acid, which inhibits growth of sugarcane . No any definite disease-causing gene has been found up to now.
Lxx is a bacterium, σ factor is one of protein subunits (α2ββ’σ) of the bacterial RNA polymerase holoenzyme and also a major regulatory factor of prokaryotic gene expression regulation. It can be used to identify the target gene promoter regions, promote the combination of the target region and RNA polymerase holoenzyme , and anti σ factors could suppress the transcription by affecting the σ factor failing to identify the promoter protein conformation or failing to form RNA polymerase holoenzyme. The first anti σ factor AsiA was cloned in 2002 , and since then many anti σ factors have been cloned subsequently [17, 18]. A transmembrane anti σ factor RseA is the product of co-transcription by downstream genes σECF and σE in Escherichia coli, which tightly binds to σE, then restricts its identification of promoter and its binding with the core enzyme of RNA polymerase . Rv0444c of Mycobacterium tuberculosis encodes anti-Sigma K factor (RskA), which could inhibit the Sigma K regulating action and affect the antigen protein expression of MPB70 and MPB83 ultimately . The σR factor (sigma R factor, SigR) activity is regulated by the anti σ factor (regulator of sigma R, RsrA) of genus Streptomyces . Previous studies showed that there are huge differences among anti σ factors in sequence and structure, and these differences led the anti σ factors to react to stimulus response and improve flexibility in bacterial transcriptional regulation .
Transcription is the first step of gene expression, and σ factor plays a key role in starting transcription, so studies on σ factor and anti σ factor are considered the key for regulating the gene expression in recent years. However, it is hard to culture Lxx in vitro, and the pathogenesis research on RSD is still at a preliminary stage. The anti σ factors of Lxx and their corresponding features and functionalities have not been sufficiently analyzed. In this study, we isolated and cloned the membrane protein gene Lxx18640 (anti-sigma K factor, RskA) of Lxx, analyzed its biological characteristics, detected its expression in infected sugarcane, and verified its expression in a heterologous system, to further discern its functional role.
Molecular characterization and bioinformatics analysis of Lxx18460
A full-length Lxx18460 cDNA was isolated from the infected sugarcane. It was 720 bp in size and the predicted molecular weight of Lxx18460 protein was 24.8 kDa with an isoelectric point (pI) of 5.01 (see Additional file 1). The sequencing results showed that its homology with the Lxx (NC_006087) from GenBank was up to 100%, and has been registered with the accession No. JQ740153.
Expression of Lxx18460 gene in Lxx-infected sugarcane
Transgenic expression of Lxx18460 in tobacco
Expression of Lxx18460 gene decreased plant height, leaf area and net photosynthetic rate in tobacco
Expression of Lxx18460 gene declined the SOD, POD, CAT and T-AOC activities in tobacco leaves
IAA, ABA and GA3 contents in tobacco leaves
Lxx18460 gene expression in transgenic tobacco plant
Lxx18460 protein expression in transgenic tobacco plant
In this study, the full-length Lxx18460 gene, the anti ơ factor of Lxx was cloned and characterized. It was found that the amino acid sequence has low homology with those from other strains, so it is speculated that most ơ factor homology is not high between different bacterial strains. Since the anti ơ factor has huge differences in structure and sequence, the bacteria could respond to the stimulus elements, which could better regulate certain gene transcriptions and expressions. Studies have shown that the concentration of Lxx in sugarcane is different in various tissues, and the Lxx titers decreasing from the basal part to the tail of stalk in sugarcane . Therefore, the expression of Lxx18460 in infected plant is related to the level of Lxx in different organs of sugarcane. We used the purified protein of Lxx18460 to obtain monoclonal antibody, which can be used for qualitative and quantitative detection of Lxx18460 protein. In addition, it can be used to further explore the colonization of the bacterium-Lxx at transcription and translation levels using Lxx18460 gene.
Li et al.  constructed the eukaryotic expression vector of TA3–13, which is a gene coding for the fragment of a cold shock protein WCP1 of wheat (Triticum aestivum L.), and transformed it into tobacco; the transgenic tobacco showed significant resistance against Tobacco mosaic virus and rot pathogen Pectobactrium carotovorum subsp. carotovorum. Tang et al.  transformed Sugarcane mosaic virus (ScMV) coat protein (CP) gene into sugarcane variety Badila, and the transgenic sugarcane of ScMV-CP gene significantly enhances the ScMV resistance of sugarcane and improve the cane yield and quality greatly. In this study, we analyzed the function of Lxx membrane protein gene Lxx18460 (anti-sigma K) by transforming it into tobacco, and then investigated the phenotypic, physiological, biochemical and molecular changes in the transgenic tobacco plants. We found that the net photosynthetic rate was lower in the transgenic tobacco than that in WT plants. The reason may be that Lxx18460 gene inhibited the expression of photosynthetic enzyme genes, and this speculation was confirmed by quantifying the relative expression of photosynthesis related genes using RT-PCR. We observed that the expression of Rubisco and PEPC genes were inhibited in the transgenic tobacco. Yu et al.  reported that the net photosynthetic rate in tomato decreased after infected with tomato yellow leaf curl virus. Sun  found that 51 down-regulated genes coded the proteins involved in photosynthesis in rice after blast infection, and the pathogen suppressed the photosynthesis genes’ expression and decreased the photosynthesis greatly. Plant could form an antioxidant protection mechanism to remove excess free radicals, and the defense enzymes of SOD, POD and CAT play a very important role in scavenging reactive oxygen species (ROS) [28, 29, 30]. Excessive expression of SOD enzyme in transgenic tobacco can increase the tolerance under stress . POD activity in alfalfa increased after infection by brownblotch strains , while CAT activity in tobacco increased after infected by pseudomonas solanacearum . In this study, the SOD, POD and CAT and T-AOC activities in the Lxx18460 transgenic tobacco declined as compared to the WT plants, which suggested that the defense capability had been decreased by transformation of Lxx18460 gene. The transcriptome analysis results also showed that the expression of catalase was decreased. The transgenic plants were more susceptible to membrane damage compared to the WT plants. The pathogen Lxx showed extremely strong infection effect though it had been diluted for hundreds of times . It might be possible that at the beginning of the infection, the expression of Lxx18460 gene in pathogenic bacteria inhibits the gene transcription of the defense enzymes and damage the defense regulation of host plants, which would help the pathogen to infect the host and to multiply inside the plant cell. Growth of plant is not only affected by the change of various enzyme activities, but also regulated by various endogenous hormones . According to the results of this study, the syntheses of GA3, IAA and ABA were inhibited in the Lxx18460 transgenic plants, which suggested that the expression of Lxx18460 gene suppresses the biosynthesis and signaling pathways of the endogenous hormones. The physiological and phenotypic changes in the transgenic tobacco proved that Lxx18460 plays a role in inhibiting the signal transduction and hormone synthesis gene expression.
It has been reported that the photosynthesis and cane yield were decreased, and defense enzyme activities and endogenous hormone levels were adversely affected by the infection of Lxx [36, 37, 38, 39]. Interestingly, the symptoms of Lxx18460 gene expression in tobacco are similar to those caused by Lxx in sugarcane. After transforming the gene Lxx18460 into tobacco, the net photosynthetic rate was decreased and the endogenous hormone synthesis inhibited, and the growth of transgenic plants hindered. As the result the plant height was lower and the leaf area smaller, subsequently, the plants become dwarf and smaller, and the biomass decreased. Furthermore, this study demonstrated that the expression level of Lxx18460 gene and Lxx18460 protein was stably increased with time course during plant growth. The results strongly supported the view that the Lxx18460 gene had been transformed into tobacco, and its expression had slowed down the growth of the transgenic tobacco plants. The higher expression showed the Lxx18460 gene, the more obvious was the effect on transgenic tobacco.
The membrane protein gene Lxx18460 of anti-sigma K (ơK) factor in Leifsonia xyli subsp. xyli in sugarcane was cloned, and its structure and expression in sugarcane infected by RSD were analyzed. The function of Lxx18460 gene was analyzed by transforming it into tobacco. The results showed that the expression of Lxx18460 gene could inhibit the growth of the transgenic tobacco. Lxx18460 gene plays an important role in regulating signal transduction, gene transcription and plant growth, and in the course of infection in sugarcane. It is important to further investigate the function of Lxx18460 gene and its role in Lxx pathogen. This is the first report of analyzing the function of the membrane protein gene Lxx18460 of anti-sigma K (σK) factor in Leifsonia xyli subsp. xyli. It is necessary to further investigate the interaction between sugarcane and Lxx using Lxx18460 gene to illustrate the molecular mechanism of Lxx infection in sugarcane.
Materials and methods
The Lxx-infected sugarcane (Saccharum spp.) variety GT11 grown in the Sugarcane Institute, Guangxi University (Nanning, China, 108°19′E 22°49’N) was used for cloning Lxx18460. The wild type tobacco (Nicotiana tabacum L. variety 346) was used for transgenic analysis.
Cloning and sequencing of Lxx18460 gene
Total RNA from the Lxx-infected sugarcane stalk was extracted using Trizol Reagent (Cowin Biosciences, Beijing, China) , and the cDNA template for RACE-PCR amplification was prepared according to the User Manual (TaKaRa, Dalian, China). Based on the sequence of Lxx18460 from the GenBank (NC-006087), we design the specific primers as R1: 5’-ATGACCGACGACGAGCGGGG-3′ and R2: 5’ TCAGGAGGCGATGCCTACGA-3′ using Primer Premier 5.0 software. The putative full length Lxx18460 gene was obtained using RT-PCR technique. The reaction procedure was as follows: 10 min at 95 °C for degeneration, 40 cycles for 40 s at 94 °C, for 40 s at 62 °C, for 90 s at 72 °C, and a final extension for 10 min at 72 °C. The amplified target bands were recovered and purified with a Gel Extraction Kit (TaKaRa, Dalian, China), and then attached to pMD18-T vector (TaKaRa, Dalian, China), transformed into DH5a competent cells (Transgene, Beijing, China); and the positive clones were identified by sequencing.
BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) was used to analyze the sequence in the NCBI database. The physical properties of the Lxx18460 protein were predicted using Expasy (http://web.expasy.org/protparam/). Conservative structure and function of the gene encoded protein domains were analyzed using NCBI CDD (Conserved Domain Databases), SMART (http://smart.embl-heidelberg.de/). Transmembrane structures of the protein were analyzed using TMPRED Server (http://embnet.vital-it.ch/software/TMPRED_form.html) and TMHMM2.0 (http://www.cbs.dtu.dk/services/TMHMM/).
Quantitative real-time PCR (qRT-PCR) analysis of Lxx18460 expression in Lxx-infected sugarcane and transgenic tobacco
Quantitative real-time PCR technique was applied to detect the expression of Lxx18460 in different stalks and leaves during the mature growth stages. Internodes 1–7 represent the first to the seventh internode form the base of sugarcane, and leaves + 1 to leaf + 5 are the first to the fifth leaf with visible dewlap from sugarcane morphological upper part on the ground. The qRT-PCR was done according to the method outlined by Zhu et al. . We calculated the relative level of gene expression using the 2−ΔΔCt formula . For transgenic tobacco, the Lxx18460 gene expression was measured at 50 to 110 days after emergence. The GAPDH sugarcane gene (accession number EF189713) and GAPDH tobacco gene (accession number M14419) were used as the internal controls to quantify the relative transcript levels in Lxx-infected sugarcane and transgenic tobacco, respectively [43, 44].
Lxx18460 protein prokaryotic expression, tobacco Lxx18460 transformation and transgenic plant generation
The Lxx18460 expression vector primers were designed according to the Lxx18460 gene ORF. EcoR I endonuclease site, Xho I endonuclease site and protective base pair were added to the 5′ terminal of the primer for gene expression detection. The prokaryotic expression vector pET-30a was transformed into E. coli BL21(DE3)pLysS. After the IPTG induction process, the protein was isolated, purified and analyzed by SDS-PAGE with Image Lab 5.0 software. The protein was identified and confirmed by mass spectrometry (MALDI-TOF). The protein induced and over-expressed in E. coli was used as antigen to produce monoclonal antibody. ELISA test was applied to analyze the titer. The specificity was analyzed using Western blot in bacteria and in the healthy and Lxx-infected sugarcane plant samples.
The full length open reading frame (ORF) of Lxx18460 was cloned using PCR technique, and then inserted into a pBI121 vector (conserved by our lab). This vector was under control of cauliflower mosaic virus 35S (CaMV35S) promoter and nopaline synthase (NOS) terminator . The recombinant plasmid pBI121-Lxx18460 construction was introduced into the Agrobacterium tumefaciens strain EHA 105, and then transformed into the WT tobacco callus as described by Horsch et al. .
The tobacco seeds were sterilized with 70% ethanol for 30 s and then rinsed with sterile ddH2O two times, followed by disinfection for 8–10 min in 4% sodium hypochlorite, rinsing with sterile ddH2O five times before they were sown in plate culture medium (M1) based on MS medium  supplemented with 25 g L− 1 sucrose and 0.7% agar (pH 5.8). The infected explants were co-cultured on M1 medium complemented with 1 mg L− 1 6-benzyladenin (BA) and 0.5 mg L− 1 indoleacetic acid (IAA), which is defined as M2 medium, at 25 °C in dark for 4 days. Then the co-cultured plants were rinsed with ddH2O containing 300 mg L− 1 cephalosporin. Positive transgenic plants were selected on M2 medium containing 100 mg L− 1 of kanamycin. The kanamycin-resistant shoots were transferred to M3 selection medium (M1 medium plus 0.5 mg L− 1 IAA, 75 mg L− 1 kanamycin) for rooting. A few days later, the non-transgenic and transgenic status of the T1 plantlets was confirmed by PCR and Western blot analysis.
The T1 plants were just used for phenotypic observation. Seeds of wild type and the transgenic T1 plants were harvested and used for further experiments. The expression of Lxx18460 in the independent T2 generation was also determined by PCR and Western blot. For physiological and biochemical analysis, the seedlings were grown in the glasshouse condition. During the T2 generation growth period, Lxx18460 expression was observed and analyzed in the transgenic and WT plants using the same method as described above.
PCR identification of transgenic tobacco
The genomic DNA was extracted from the leaves of transgenic and WT tobacco plants using a modified SDS extraction method , and tested for the presence of target gene of Lxx18460 and marker gene of NPTII. The PCR reaction and PCR procedure was described in Additional file 5.
Western blot analysis of Lxx18460 in transgenic tobacco
Total protein was extracted from the leaves of transgenic and WT tobacco plants using a modified phenol extraction method , and quantified by spectrophotometer. Western blot was processed as described by Zhu et al. .
Plant height, leaf area and net photosynthetic rate measurements
Plant height was measured from the transgenic and WT tobacco stalk at 50, 80, and 110 days after emergence, respectively. Leaf area was measured using CI-203 Handheld Leaf Area Meter (CID Bio-Science, Inc., Camas, USA). Net photosynthetic rate was measured using Li-6400 photosynthesis system (LI-COR, Lincoln, USA).
Activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and total antioxidant capacity (T-AOC) assay
Activities of SOD, POD, CAT and T-AOC were measured from leaves of the transgenic and WT tobacco at 50, 80 and 110 days after emergence, respectively. The enzymes were extracted from 0.5 g leaf and grounded in 2 mL extraction buffer containing 0.1 M phosphate buffer (pH 7.4) and 1% polyvinylpyrolidone using a mortar and pestle kept in ice bath. The homogenate was centrifuged at 10,000×g for 10 min at 4 °C and the supernatant was used for enzyme assay. The SOD activity was measured using the nitro-blue tetrazolium method . The activity of POD was measured using the guaiacol method . The activities of CAT and T-AOC were measured using CAT and T-AOC Assay Kit (Jiancheng, Nanjing, China) according to the User Manual.
Contents of IAA, ABA and GA3 assay
The fresh leaves of transgenic and WT tobacco were sampled at 50, 80 and 110 days after emergence, respectively. The plant hormone was extracted from 1 g leaf with 2 mL extract buffer (80% methanol containing 1 mM butylated hydroxytoluene) and grounded into homogenate in ice bath. Then solution containing 0.1% Tween-20 and 0.1% gelatin phosphate buffer, pH 7.5) was used to dissolve the hormone extract. The extracts were analyzed using ELISA method with five repeats .
BGISEQ-500 sequencing and transcriptome analysis
Total RNA of each sample was extracted using the Trizol Reagent (Cowin Biosciences, Beijing, China) according to the manufacturer’s protocol from three biological replicates of each treatment (WT and Transgenic) resulting in 6 samples. The total RNA concentration of each sample was estimated using a Agilent 2100 Bioanalyzer (Agilent Technologies Inc.). The integrity of the RNA samples was assessed with 1.2% agarose gel electrophoresis. The six samples were sequenced using BGISEQ-500 Sequencing performed by Beijing Genomics Institute (BGI)-ShenZhen, China according to the manufacturer’s instructions. Detailed BGISEQ-500 sequencing experimental process and transcriptome data analysis method refer to Additional file 6.
All the data were analyzed using Microsoft Excel 2003 (Microsoft) and IBM SPSS 21.0. All data points represent means from 15 plants of transgenic or WT tobacco. There were three technical replicates for each experiment, and statistical differences were compared based on the Duncan’s test with P values set at 0.05 and 0.01 levels, respectively, and means were compared by standard error (SE).
We acknowledge Mr. Dajie Zhou from Beijing Genomics Institute (Shenzhen) for the assistance of transcriptome sequencing.
The present study was supported by National Natural Science Foundation of China (31360293), International Scientific Cooperation Program of China (2013DFA31600), Guangxi Special Fund for Scientific Base and Talent (GKAD17195100), Guangxi Special Funds for Bagui Scholars and Distinguished Experts (2013–3), Guangxi Natural Science Fund (2011GXNSFF018002, 2012GXNSFDA053011), and Guangxi Sugarcane Innovation Team of National Agricultural Industry Technology System (gjnytxgxcxtd-03-01). The funding bodies played no role in the design, execution, interpretation of the data, or writing of the manuscript.
Availability of data and materials
Clean data (FASTQ files) for all samples can be accessed in the NCBI Sequence (https://www.ncbi.nlm.nih.gov/sra) under the accession number of PRJNA498357.
L.T.Y. and Y.R.L. designed the study. K.Z., M.S., D.Z., and Y.X.X. conducted the experiments. K.Z. analyzed the data. K.Z., L.T.Y. and Y.R.L. wrote the manuscript. L.T.Y. and Y.R.L. revised and finalized the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
No specific permits were required for the described field studies. The location is not privately-owned or protected in any way, and the field studies did not involve endangered or protected species.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
- 1.Li YR. Modern sugarcane sciences. Beijing: China Agriculture Press; 2010.Google Scholar
- 2.Li YR, Yang LT. Sugarcane agriculture and sugar industry in China. Sugar Tech. 2015. https://doi.org/10.1007/s12355-014-0342-1.
- 4.Haapalainen M, Mattinen J, Metzler MC. The growth of a plant-parasitic bacterium, Clavibacter xyli subsp cynodontis, is enhanced by xylem fluid components. Physiol Mol Plant Pathol. 2000. https://doi.org/10.1006/pmpp.2000.0260.
- 5.Dean JL. Single-stool plots for estimating relative yield losses caused by ratoon stunting disease of sugarcane. Plant Dis. 1983. https://doi.org/10.1094/PD-67-47.
- 6.Grisham MP. Effect of ratoon stunting disease on yield of sugarcane grown in multiple three-year plantings. Phytopathology. 1991. https://doi.org/10.1094/Phyto-81-337.
- 7.Li WF, Shen K, Huang YK, Wang XY, Luo ZM, Ying XM, Yin J, Ma L, Shan HL, Zhang RY. PCR detection of ratoon stunting disease pathogen and natural resistance analysis in sugarcane core germplasms. Crop Prot. 2013. https://doi.org/10.1016/j.cropro.
- 8.Young AJ. Possible origin of ratoon stunting disease following interspecific hybridization of Saccharum species. Plant Pathol. 2016. https://doi.org/10.1111/ppa.12545.
- 10.Quecinea MC, Silvaa TMG, Carvalhoa SS, Mondina M, Teixeira-Silva NS, LEA C, Monteiro-Vitorello CB. A stable Leifsonia xyli subsp. xyli GFP-tagged strain reveals a new colonization niche in sugarcane tissues. Plant Pathol. 2015. https://doi.org/10.1111/ppa.12397.
- 11.Fegan M, Croft BJ, Teakle DS. Sensitive and specific detection of Clavibocter xyli subsp. xyli, causative agent of ratoon stunting disease of sugarcane, with a polymerase chain reaction-based assay. Plant Pathol. 1998. https://doi.org/10.1046/j.1365-3059.
- 12.Monteiro-Vitorello CB, Camargo LE, Van Sluys MA, Kitajima JP, Truffi D, do Amaral AM, Harakava R, de Oliveira JC, Wood D, de Oliveira MC, Miyaki C, Takita MA, da Silva AC, Furlan LR, Carraro DM, Camarotte G, Almeida NF Jr, Carrer H, Coutinho LL, El-Dorry HA, Ferro MI, Gagliardi PR, Giglioti E, Goldman MH, Goldman GH, Kimura ET, Ferro ES, Kuramae EE, Lemos EG, Lemos MV, Mauro SM, Machado MA, Marino CL, Menck CF, Nunes LR, Oliveira RC, Pereira GG, Siqueira W, de Souza AA, Tsai SM, Zanca AS, Simpson AJ, Brumbley SM, Setúbal JC. The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli. Mol Plant Microbe Interact. 2004. https://doi.org/10.1094/MPMI.2004.17.8.827.
- 15.Chan ZL, Wang Q, Xu XB, Meng XH, Qin GZ, Li BQ. Functions of defense-related proteins and dehydrogenase in resistance response induced by salicylic acid in sweet cherry fruits at different maturity stages. Proteomics. 2008. https://doi.org/10.1002/pmic.
- 16.Urbauer JL, Simeonov MF, Urbauer RJ, Adelman K, Gilmore JM, Brody EN. Solution structure and stability of the anti-sigma factor AsiA: implications for novel functions. PNAS. 2002. https://doi.org/10.1073/pnas.032464699.
- 17.Park ST, Kang CM, Husson RN. Regulation of the SigH stress response regulon by an essential protein kinase in Mycobacterium tuberculosis. PNAS. 2008. https://doi.org/10.1073/pnas.0801143105.
- 18.Lourenço RF, Kohler C, Gomes SL. A two-component system, an anti-sigma factor and two paralogous ECF sigma factors are involved in the control of general stress response in Caulobacter crescentus. Mol Microbiol. 2011. https://doi.org/10.1111/j.1365-2958.
- 19.Campbell EA, Tupy JL, Gruber TM, Wang S, Sharp MM, Gross CA, Darst SA. Crystal structure of Escherichia coli σE with the cytoplasmic domain of its anti-σ RseA. Mol Cell. 2003. https://doi.org/10.1016/S1097-2765(03)00148-5.
- 20.Said-Salim B, Mostowy S, Kristof AS, Behr MA. Mutations in Mycobacterium tuberculosis Rv0444c, the gene encoding anti-SigK, explain high level expression of MPB70 and MPB83 in Mycobacterium bovis. Mol Microbiol. 2006. https://doi.org/10.1111/j.1365-2958.2006.05455.x.
- 22.Bai H, Yan H, Hou Z, Yu Q, Luo XX. The research progress on the units of bacteria RNA polymerase. Prog Physiol Sci. 2011;42:47–51.Google Scholar
- 23.Grisham MP, Pan YB, Richard JEP. Early detection of Leifsonia xyli subsp. xyli in sugarcane leaves by real-time polymerase chain reaction. Plant Dis. 2007; doi: https://doi.org/10.1094/PDIS-91-4-0430.
- 25.Tang XD. The evaluation of ScMV-CP gene transgenic sugarcane. Fujian Agriculture and Forestry University. Master's thesis. 2009;Google Scholar
- 26.Yu L, Guo SR, Zhu WM, Yan J, Hei YX. Effects of tomato yellow leaf curl virus on photosynthetic characteristics and chloroplast ultra-structure of the tomato leaves. Acta Botan Boreali-Occiden Sin. 2011;31:1355–9.Google Scholar
- 27.Sun LX. SAGE analysis of transcriptome response in rice leaves during host-fungal pathogen interactions. Dissertation, Institute of Plant and Ecology, SIBS, CAS. 2005;Google Scholar
- 28.Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot. 2003. https://doi.org/10.1093/aob/mcf118.
- 30.Huang XS, Liu JH, Chen XJ. Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes. BMC Plant Biol. 2010. https://doi.org/10.1186/1471-2229-10-230.
- 31.Gupta AS, Heinen JL, Holaday AS, Burke JJ, Allen RD. Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. PNAS.1993; doi: https://doi.org/10.1073/pnas.90.4.1629.
- 32.Yuan QH, Gui Z, Zhang WS. Comparison of the activities of SOD, POD and PPO within alfalfa cultivars resistant and susceptible to alfalfa common leaf. Acta Prataculturae Sinica. 2002;11:100–4.Google Scholar
- 33.Ke YQ, Pan TG, Fang SM. Effect of Pseudomonas solanacearum infection on H2O2 metabolism and chlorophyll fluorescence parameters and their relations to disease resistance. Chin J Eco-Agric. 2002;10:36–9.Google Scholar
- 34.Li PP, Zhang X, Pan XM, Wang JS, Song CF. His-tag fusion expression, purification and TMV resistance induction of truncated wheat cold shock protein gene fragment TA3–13. J Nanjing Agric Univ. 2010;33:54–8.Google Scholar
- 35.Fan JJ, Li XM, Ruan YH. Changes in endogenous hormone contents during grain filling in hybrid rice and its parents. Plant Physiol Commun. 2004;40:146–8.Google Scholar
- 36.Chen MH, Huang X, Xie XN, Yang LT, Li YR, Chen BS. Effects of ratoon stunting disease on growth and endogenous hormone in sugarcane. J Huazhong Agric Univ. 2013;32:57–61.Google Scholar
- 38.Zhang XQ, Liang YJ, Tang YX, Shao M. Morphological characteristic of Leifsonia xyli subsp. Xyli and its effects on photosynthesis of sugarcane. J Huazhong Agric Univ. 2015;34:42–7.Google Scholar
- 39.Zhang XQ, Liang YJ, Zhu K, Wu CX, Yang LT, Li YR. Influence of Inoculation of Leifsonia xyli subsp. Xyli on Photosynthetic Parameters and Activities of Defense Enzymes in Sugarcane. Sugar Tech. 2016. https://doi.org/10.1007/s12355-016-0479-1.
- 40.Hogenhout SA, Redinbaugh MG, Ammar ED. Plant and animal rhabdovirus host range: a bug’s view. Trends Microbiol. 2003. https://doi.org/10.1016/S0966-842X(03)00120-3.
- 41.Zhu K, Yuan D, Zhang XQ, Yang LT, Li YR. The physiological characteristics and associated gene expression of sugar cane inoculated with Leifsonia xyli subsp. xyli. J Phytopathol. 2018. https://doi.org/10.1111/jph.12659.
- 42.Livak K, Schmittgen T. Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods. 2001. https://doi.org/10.1006/meth.
- 43.Li J, Phan TT, Li YR, Xing YX, Yang LT. Isolation, transformation and overexpression of sugarcane SoP5CS gene for drought tolerance improvement. Sugar Tech. 2017. https://doi.org/10.1007/s12355-017-0568-9.
- 44.Phan TT, Sun B, Niu JQ, Tan QL, Yang LT, Li YR. Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco. Plant Cell Rep. 2016. https://doi.org/10.1007/s00299-016-2004-0.
- 45.Deng L, Liu H, Yang WN. Construction of the recombinant expression plasmid pBI121-GFP-62390 and pBI121-GFP-51780 containing green fluorescent protein gene. J Anhui Agric Sci. 2008;36:11260–2.Google Scholar
- 48.Shan Z, Wu HL, Li CL, Chen H, Wu Q. Improved SDS method for general plant genomic DNA extraction. Guangdong Agric Sci. 2011;8:113–5.Google Scholar
- 49.Yang L, Lin H, Takahashi Y, Chen F, Walker MA, Civerolo EL. Proteomic analysis of grapevine stem in response to Xylella fastidiosa inoculation. Physiol Mol Plant Pathol. 2011. https://doi.org/10.1016/j.pmpp.2010.11.002.
- 50.Li HS, Sun Q, Zhao SJ, Zhang WH. Principle and technology of physiological and biochemical test in plants. Beijing: Higher Education Press; 2000. p. 195–261.Google Scholar
- 51.Yang LT, Li YR, Mo JR. Preliminary study on effects of several physiological and biochemical characteristics of sugarcane leaves after the application of “duo Xiao Hao”. J Guangxi Agric Coll. 1990;9:79–83.Google Scholar
- 52.Wu SR, Chen WF, Zhou X. Enzyme linked immunosorbent assay for endogenous plant hormones. Plant Physiol Commun. 1988;53:57.Google Scholar
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