Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family
The western corn rootworm, Diabrotica virgifera virgifera, is a pervasive pest of maize in North America and Europe, which has adapted to current pest management strategies. In advance of an assembled and annotated D. v. virgifera genome, we developed transcriptomic resources to use in identifying candidate genes likely to be involved in the evolution of resistance, starting with members of the ATP-binding cassette (ABC) transporter family.
In this study, 65 putative D. v. virgifera ABC (DvvABC) transporters were identified within a combined transcriptome assembly generated from embryonic, larval, adult male, and adult female RNA-sequence libraries. Phylogenetic analysis placed the deduced amino-acid sequences of the DvvABC transporters into eight subfamilies (A to H). To supplement our sequence data with functional analysis, we identified orthologs of Tribolium castaneum ABC genes which had previously been shown to exhibit overt RNA interference (RNAi) phenotypes. We identified eight such D. v. virgifera genes, and found that they were functionally similar to their T. castaneum counterparts. Interestingly, depletion of DvvABCB_39715 and DvvABCG_3712 transcripts in adult females produced detrimental reproductive and developmental phenotypes, demonstrating the potential of these genes as targets for RNAi-mediated insect control tactics.
By combining sequence data from four libraries covering three distinct life stages, we have produced a relatively comprehensive de novo transcriptome assembly for D. v. virgifera. Moreover, we have identified 65 members of the ABC transporter family and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species.
KeywordsATP-binding cassette (ABC) transporter Phylogenetic Transcriptome RNA interference (RNAi) Corn rootworm
National Center of Biotechnology Information
Open reading frames
The western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), is a major pest of maize in Europe and North America [1, 2, 3], where costs of management, as well as crop losses attributed to damage by this pest, are estimated at over 1 billion U.S. dollars annually in North America alone (reviewed in ). The notorious difficulty facing efforts to control D. v. virgifera feeding on maize has arisen via intra-species adaptations that overcome various pest management methods . For example, changes in oviposition preference within “soybean variant” populations of D. v. virgifera in the Midwest United States circumvent the cultural-control practice of corn-soybean rotation [3, 5, 6, 7]. Additionally, adapted phenotypes within North American D. v. virgifera populations can survive high exposures to organochlorine , pyrethroid , and carbamate and organophosphate insecticides . In some instances resistant phenotypes have persisted for decades despite the removal of selection pressures . More recently, field populations of D. v. virgifera have developed high levels of resistance to transgenic maize hybrids that express Bacillus thuringiensis (Bt) crystal toxins Cry3Bb1 , mCry3A , Cry3.1Ab , and Cry34/35Ab1 [14, 15, 16, 17]. However, RNA interference (RNAi) shows great potential as a novel insect pest control technology , especially in instances where target species are sensitive to oral RNAi . D. v. virgifera is highly sensitive to oral RNAi [20, 21, 22], suggesting that it could suppress feeding damage caused by this pest .
ATP-binding cassette (ABC) proteins comprise one of the largest gene families, and are found across prokaryotic and eukaryotic domains . Most of these proteins function as transmembrane transporters, which actively move a myriad of molecules across cellular membranes . ABC transporter proteins have a two-domain structure: a highly conserved nucleotide-binding domain (NBD) and a variable transmembrane domain (TMD) . The NBD binds and hydrolyzes ATP to provide the energy required for translocating a substrate across cell membranes, while the TMD forms a channel through which the substrate is transported . Each NBD possesses several highly conserved, characteristic motifs, including Walker A, Walker B, Q-loop, D-loop, H-loop, and ABC signature motifs, while each TMD is made up of five to six transmembrane α-helices that dictate substrate specificities . ABC transporter proteins require two NBDs and two TMDs for functionality. Some ABC transporters are full-transporters (FT) in that two TMDs and two NBDs are encoded in a single protein, whereas most are half-transporters (HT; one TMD and one NBD) and form functional units following homodimerization or heterodimerization [24, 27, 28]. Due to the relatively conserved sequence of the NBD, it has been used for the phylogenetic classification of the ABC transporter superfamily into eight subfamilies designated A to H (ABCA to ABCH) .
Among insect species, ABC transporters are implicated in diverse functions, including transportation of eye pigments [30, 31, 32, 33, 34], and resistance to chemical insecticides [35, 36]. Within the model species for Coleoptera, the red flour beetle, Tribolium castaneum, Broehan et al.  reported that RNAi-mediated knockdown of some ABC transporters resulted in mortality, or phenotypes characterized by arrested growth, abnormal cuticle formation, defective eye pigmentation, or abnormal egg-laying or -hatching. Changes in the expression level or structure of some ABCA, ABCC and ABCG subfamily members have been associated with Bt toxin resistance in species of Lepidoptera , while paralogs of an ABCB transporter were linked to Bt Cry3Aa resistance in the coleopteran species, Chrysomela tremula , and were found to be in proximity to a quantitative trait locus (QTL) for Cry3Bb1 resistance in D. v. virgifera .
Similar investigations of ABC transporters in D. v. virgifera are arguably limited due to the dearth of genomic resources available for this species, which are currently comprised of Sanger and Roche 454 read-based transcriptome assemblies [40, 41, 42, 43]. Complicating the development of genomic tools is the 2.58 GB size and complex repetitive structure of the D. v. virgifera genome [4, 44]. Regardless, RNA sequencing (RNA-seq) has become an expeditious and cost-effective method for obtaining a wealth of transcriptome sequence data in non-model insects . In the following, a de novo transcriptome assembly approach was used for the first prediction, annotation, and functional analysis of the ABC transporter gene family in D. v. virgifera. Specifically, eight ABC transporters were identified as putative orthologs to those previously reported to have a defining RNAi phenotype in the model coleopteran species, T. castaneum  (DvvABCA_50718, DvvABCB_39715, DvvABCE_2830, DvvABCF_2701, DvvABCG_3712, DvvABCG_14042, Dvvw and DvvABCH_5118). Subsequent RNAi-mediated knockdown demonstrated conservation of function with T. castaneum, as well as established potential new insecticidal targets for the control of this devastating agricultural pest.
Transcriptome sequencing, assembly, and annotation
Paired-end RNA-sequencing libraries and sequencing
Raw read data
Trimmed read data
600 to 700-bp
600 to 700-bp
600 to 700-bp
600 to 700-bp
Bioinformatic analysis of the D. v. virgifera ABC transporter family
Classification of D. v. virgifera ATP binding cassette (ABC) transporters
Diabrotica virgifera virgifera transcript
Nearest Tribolium castaneum ortholog
Gene expression across developmental stages
Results of RNAi knockdown of selected ABC transporters
Deformed wings & elytra
Defect in pupal-adult molt
Malformed ovaries; low egg lay
Lethal; pupal developmental arrest
Prevented embryonic development
Pigmentation defect; white eyes
Lethal at molting
Lethal pupal developmental arrest
Lethal at molting
RNAi knockdown phenotypes
RNAi-mediated knockdown of DvvABCE_2830 and DvvABCF_2701 in larvae resulted in 100% mortality. Prior to death, it was noted that the body mass of treated individuals was less than that of similarly-aged larvae treated with buffer alone (Fig. 4g, h). Analogously, injection of DvvABCE_2830 and DvvABCF_2701 dsRNA separately into pre-pupae both caused 100% mortality with no adult eclosion (results not shown). Injection of dsRNA specific for DvvABCH_5118 into early-instar D. v. virgifera larvae and pre-pupae caused development to arrest as individuals prepared to molt, thus resulting in 100% mortality (Fig. 4f). Affected individuals appeared to desiccate prior to death (personal observation).
Injection of dsRNA targeting DvvABCG_3712, DvvABCG_14042, and Dvvw resulted in phenotypes similar to those seen with RNAi knockdown of the corresponding T. castaneum orthologs . Specifically, injection of dsRNA targeting Dvvw, gave the expected white-eye phenotype (Fig. 4e); indeed, we had identified this white ortholog previously . Injection of DvvABCG_3712 dsRNA into pre-pupae caused developmental defects that resulted in 80% mortality (Table 3; Fig. 4c). Interestingly, adult females treated with DvvABCG_3712 dsRNA produced fewer eggs compared to females injected with buffer alone (Fig. 5b), and the eggs that were laid lacked obvious signs of embryonic development (Fig. 4i) and ultimately failed to hatch (Additional file 6: Figure S4). Injection of DvvABCG_14042 dsRNA into larvae and pre-pupae resulted in molting defects; about 80% of these died during their next molt (Table 3), while the 20% that survived through subsequent larval molts died following pupation (Fig. 4d).
In recent years, ABC transporters have become a major focus for research in arthropods. This is in part due to their overall role in xenobiotic transport and insecticide resistance [25, 47, 48, 49, 50], but more specifically, due to their suspected role in susceptibility to Bt toxins [38, 51, 52]. For example, Gahan et al.  reported genetic linkage of Heliothis virescens HvABCC2 with resistance to Cry1Ac, while changes in the structure, splicing, or expression level of ABCC2 orthologs were later associated with Cry1Ac resistance in Helicoverpa armigera , Bombyx mori , and Spodoptera exigua . Indeed, expression of the P. xylostella ABCC2 ortholog in Drosophila melanogaster conferred susceptibility to this lepidopteran-specific toxin . An ABCC2 ortholog is also linked to Cry1F resistance in Ostrinia nubilalis  and S. frugiperda . Additionally, structural mutations in a member of subfamily A, HaABCA2, were implicated in Cry2Ab resistance in H. armigera , and, more recently, researchers were able to recapitulate an ABCA2 resistance allele in a susceptible population of H. armigera , providing further evidence for the importance of normal ABCA2 function in Cry2Ab toxicity. Reduced expression of ABCG members have been associated with Cry1Ac resistance in P. xylostella , as well as Cry1Ac and Cry1Ab resistance in O. furnacalis . More recent studies in species of Coleoptera have implicated ABCB subfamily members in Cry3Aa resistance in C. tremula  and in Cry3Ab1 resistance in D. v. virgifera .
The study of ABC transporters in several arthropod species have relied on genomic data, including T. castaneum , Aethina tumida , B. mori , D. melanogaster , Bemisia tabaci , Daphnia pulex , and Tetranychus urticae . Due to the status of D. v. virgifera as a major pest of cultivated maize (see Introduction) and current fragmented state of the unpublished draft genome assembly of this species (GenBank accession PXMJ00000000.2), the Illumina-based transcriptome assemblies reported here represent a particularly valuable genetic tool for gene discovery, characterization, and genome annotation. In particular, the 65 ABC transporter genes we identified are expected to be useful in downstream studies on insecticide resistance traits in D. v. virgifera.
Broehan et al.  previously identified 73 ABC transporters in T. castaneum, and a 74th ABC transporter was more recently reported by Grubbs et al. . There are several possible reasons for why the 65 DvvABC transporters we identified are comparatively fewer than in T. castaneum. Firstly, our transcriptome was derived from lower-throughput sequencing data (Illumina MiSeq), therefore genes expressed at very low levels may not have been represented within our raw Illumina data. Secondly, our RNA-seq libraries were not comprehensive of all possible life/growth stages or conditions, such that transcripts not expressed during growth states or under conditions used in this study would have been missed. Regardless, BLASTx analyses of the 65 putative D. v. virgifera ABC transporters identified in this study demonstrate their greatest sequence similarity to T. castaneum and A. glabripennis orthologs. This is probably a consequence of the extensive publicly available genomic data for both T. castaneum and A. glabripennis, as well as their close phylogenetic relationships to D. v. virgifera. Furthermore, the putative one-to-one relationship among orthologs from D. v. virgifera and T. castaneum may suggest the retention of copy number without extensive gene loss or gain across evolutionary time.
Despite the relatively large amount of genomic and transcriptomic data available for model and some non-model coleopteran species, there is a comparative overall dearth of functional data available to support automated computational annotations. To partially address this shortfall, we generated functional information based on RNAi knockdown of eight D. v. virgifera ABC transporters, each of which demonstrated fairly conserved roles relative to their T. castaneum orthologs . While some D. v. virgifera RNAi-mediated loss-of-function phenotypes include visible developmental defects, such as loss of eye pigmentation, others cause growth arrest and/or death. For example, knockdown of DvvABCA_50718 led to death during the pupal-to-adult molt and also caused deformation of wings and elytra in surviving adult beetles, which was the same as previously seen in T. castaneum  (Table 3; Fig. 4a). Since subfamily A transporter members are implicated in mammals with lipid transport, which can impact cell physiology , it is conceivable that the effects of the knockdown of DvvABCA_50718, and of its homologs, TcABCA-9A/9B, in T. castaneum , could be the result of disrupting critical lipid transport. DvvABCB_39715 RNAi also recapitulated the lethal effects of its T. castaneum ortholog; the effects on female fecundity could make this gene a particularly interesting target for RNAi-based pest control. It is worth noting that D. v. virgifera is predicted to have one more ABCB HT subfamily member compared to other insects , especially other beetles [30, 64]. While ABCB FTs have been implicated in chemical insecticide resistance among insects , HTs are known to be mitochondrial transporters in humans, with roles in iron metabolism and transportation of Fe/S protein precursors [68, 69]. These possibilities were outside the scope of our research, but future investigations into the function of DvvABCBs could be beneficial for deciphering mechanisms of resistance evolution in D. v. virgifera.
RNAi knockdown of DvvABCE_2830 and DvvABCF_2701 resulted in 100% larval mortality. ABCE and ABCF subfamilies are highly conserved across all phyla, and due to their lack of TMDs are considered non-transporters. Instead, they appear to play roles in regulating translation [70, 71], indicating that ABCE and ABCF proteins are essential. Thus, given that these genes are highly conserved across taxa in sequence, function, and RNAi phenotype , it may not be surprising that lethal RNAi knockdown phenotypes were obtained in D. v. virgifera.
The phenotypes observed following independent RNAi knockdown of DvvABCG_14042 and DvvABCH_5118 involved molting defects that resulted in near complete mortality. While these results are consistent with functional analysis of their T. castaneum orthologs, RNAi knockdown of the DvvABCG_14042 homolog TcABCG-8A in T. castaneum produced an additional phenotype of premature development of compound eyes . In contrast, we did not observe any analogous eye phenotypes in D. v. virgifera following RNAi knockdown. It is likely that since the injected D. v. virgifera larvae died prior to reaching the next stage of development, there was no opportunity for compound eyes to form. In other species, orthologs of DvvABCH_5118 are known to transport cuticular lipids that are deposited in the outer epicuticle layer to form a waterproof barrier [30, 62]. Therefore, it could be that cuticular lipid deposition may be reduced following RNAi knockdown of this ABCH transporter, which could promote desiccation and subsequent mortality of affected individuals.
The ABCG proteins are HTs, and, with 12 predicted members, form the second largest subfamily of ABC transporters identified in D. v. virgifera (Table 2). Among insects, some of the first ABCGs to be characterized were the pigment transporters (white, scarlet and brown) in D. melanogaster [34, 72]. Mutants of white are characterized by white eyes (i.e. complete loss of eye pigmentation), scarlet mutants by bright red eyes (i.e. loss of brown pigments), and brown mutants by dark brown eyes (i.e. loss of red pigments) . Studies have revealed that some ABCG proteins perform other crucial physiological roles in the transport of lipids, sterols, and drugs . In the current study, RNAi-mediated knockdown of Dvvw resulted in a white-eyed phenotype consistent with prior observations in T. castaneum , and with our own previous findings in D. v. virgifera . Our findings support a prediction that Dvvw is part of the ommochrome pathway, where it is likely acting within a heterodimeric complex to import ommochrome pigments into the pigment granules of the compound eye. As mentioned above, loss of white function in D. melanogaster, results in white-eyed flies, while mutations in scarlet lead to red-eyed flies. However, RNAi-mediated knockdown of the corresponding gene, ABCG-9A (scarlet), in T. castaneum produces white-eyed beetles [30, 32]. This finding was not surprising, since a previous report of RNAi targeting vermilion, a pivotal gene in the ommochrome pathway, also generates a white-eyed phenotype in T. castaneum , leading the authors to conclude that the T. castaneum eye is pigmented by ommochromes alone, and that the ommochrome biosynthetic pathway in T. castaneum produces red pigments as end products, rather than brown pigments as in D. melanogaster. Unfortunately, our initial survey of the D. v. virgifera transcriptome failed to identify a scarlet ortholog in our DNASTAR assembly, thus its function was not assessed. We did identify a scarlet ortholog from the Trinity assembly (See Table 2 and Additional file 5: Figure S3) after we had completed our functional analyses, but we were still unable to find any evidence of a brown ortholog. So, it will be interesting to investigate in future studies if pigmentation of the D. v. virgifera eye is more similar to that of T. castaneum or D. melanogaster. Specifically, in D. melanogaster a third ABCG transporter, brown, is required for wild-type pigmentation of the eye. In flies, Brown heterodimerizes with White and transports pteridine-based pigments into the eye. Although an ortholog of brown has been identified in the T. castaneum genome, no function has been identified .
This study provides a relatively large transcriptomic resource comprising genes expressed across several life stages of the arthropod pest species, D. v. virgifera. Due to potential omission of orthologs from our assembly, undoubtedly additional research will need to be performed in order to identify the full compliment of ABC transporters encoded by D. v. virgifera, and further functional assays will be needed to validate putative biochemical roles. Regardless, our work represents the initial description of the ABC transporter gene family in D. v. virgifera. Furthermore, the knockdown of ABC transporters DvvABCB_39715 and DvvABCG_3712, each of which reduced egg production and/or prevented embryonic development, could provide novel targets for D. v. virgifera population suppression and use as an insecticidal control agent. This research is a contribution to a growing set of genomic resources for arthropods, and provides information that may facilitate the development of methods to enhance the control of a devastating agricultural pest species.
All D. v. virgifera used in this study are nondiapausing, from a colony previously established at North Carolina State University using beetles obtained from both Dr. Wade French (USDA-ARS-NGIRL, Brookings, SD) and Crop Characteristics, Inc. (Farmington, MN, USA) (see ). Eggs deposited in an oviposition chamber (agar plate with cheese cloth) were collected weekly, pipetted into soil-filled containers, and held at 26 °C for 1 week. Larvae were reared on roots of germinated corn seed in 16-oz containers, while adults were maintained in a 30cm3 BugDorm (MegaView Science, Taiwan) at 26 °C, 70% relative humidity with an L14:D10 photoperiod and fed an artificial diet (Western Corn Rootworm w/o Pollen Substitute, Frontier Insect Diet, Newark, DE, USA). Injected individuals were reared in small containers with corn seedlings to allow downstream observation.
Transcriptome sequencing, assembly, and annotation
Total RNA was extracted from mixed-staged D. v. virgifera embryos (n = 500 from an overnight egg lay aged up to 14 days), mixed-stage larvae (first-instar larvae (n = 20); second-instar larvae (n = 10); and third-instar larvae (n = 2), as well as an adult male, and an adult female (n = 1 each) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) and treated with DNase I (Qiagen) according to the manufacturer’s instructions. The isolated total RNA was submitted to the Genomic Sciences Laboratory (North Carolina State University, NC, USA) for quality assessment, poly(A) selection, fragmentation, selection of ~ 650 bp fragment sizes, Illumina TruSeq® library preparation, and 300 bp paired-end sequencing on an Illumina MiSeq sequencer (Illumina, San Diego, CA, USA).
Raw FASTQ reads for each library were assessed using FastQC . Reads were initially imported into SeqMan NGen ® (DNASTAR, Madison, WI, USA), where onboard scripts were used to quality trim and de novo assemble reads into contigs using default settings. Additionally, raw reads from individual libraries were trimmed of Illumina adapter sequence contamination, bases having Phred quality score < 20 (q < 20), and sequence reads < 35 bp using Trimmomatic 0.32 . Resulting trimmed read pairs from each library were concatenated into single R1- and R2-specific FASTQ files using a custom PERL script, and then assembled into contigs using SOAPdenovo-Trans v 1.0.3  (asm-flags = 0; max_rd_len = 301; map_len = 75; avg_ins = 700; kmer (−K = 127)). Trimmed reads were also assembled with Trinity  using default parameters, except for adjustment for library insert length (−-group_pairs_distance = 700) and minimum read overlap (−-path_reinforcement_distance = 75). The complexity of SOAPdenovo-Trans and Trinity assemblies were reduced by clustering allelic variants using CD-HIT-EST  with default parameters, except for change of sequence identity (−c 0.95), word length (−n 10), and length of throw-away sequence (−l 11). The relative completeness of each clustered D. v. virgifera transcriptome assembly was evaluated by comparison with the universal single-copy orthologs from Arthropoda obtained from OrthoDB v 9  using BUSCO v 3  (E-value cutoff 0.001). Full- and partial-length open reading frames and corresponding derived amino-acid sequences were predicted from the resulting SOAPdenovo-Trans clusters with TransDecoder v3.0.0  using a minimum length of 100 amino acids.
The transcript sequences assembled by SeqMan NGen® (DNASTAR, Madison, WI) were imported into Blast2GO v4.0 [84, 85] and annotations acquired via BLASTx  comparison to the non-redundant (nr) arthropod-specific protein database at the National Center of Biotechnology Information (NCBI). The combined graphs were created at level 2 for Biological Process (P), Cellular Component (C), and Molecular Function (F) categories from Blast2GO.
Bioinformatic analysis of the D. v. virgifera ABC transporter family
A searchable database was created from the combined DNASTAR D. v. virgifera transcript assembly, and subsequently searched with the set of deduced T. castaneum ABC transporter amino-acid sequences [30, 32] as queries using the tBLASTn algorithm in BlastStation software (TM Software Inc., Arcadia, CA, USA). Homologous sequences were selected based on sequence identity and E-value (< 10− 6). Putative D. v. virgifera ABC sequences were then used as BLASTx queries of the non-redundant NCBI protein database using the web blast interface (https://blast.ncbi.nlm.nih.gov/Blast.cgi) to confirm their identity as insect ABC genes; those that appeared to not be of non-insect origin, or were otherwise not ABC genes, were discarded. The number and positions of transmembrane domains were assessed via query of the NCBI Conserved Domain Database . Finally, each D. v. virgifera ABC gene was putatively assigned to a subfamily (A-H) based on greatest similarity assigned to orthologs within BLASTx results. This BLAST search procedure was analogously repeated for SOAPdenovo-Trans and Trinity assemblies. The complexity of each ABC gene set was reduced by clustering allelic variants (sequence) across assemblies, and a comprehensive non-redundant set of putative D. v. virgifera ABC transporter contigs were generated (Additional file 7). Assembly of origin is denoted in sequence names as follows: DNASTAR (D), Trinity (T), and SOAPdenovo-Trans (S = “scaffold” and C = “contig”) within the FASTA files. The full translation product of each contig can be found in Additional file 8.
Phylogenetic relationships among derived D. v. virgifera ABC transporter protein sequences were reconstructed from the conserved NBD. A multiple sequence alignment was performed with MUSCLE using MEGAX  (default parameters) and used within a subsequent phylogenetic analysis. The unrooted Maximum Likelihood phylogenetic trees were constructed in the MEGAX program using default parameters in all categories except: LG model of amino-acid substitution with Gamma distributed substitution rates (based on Best Model determination within the MEGA program), Partial Deletion treatment of gaps/missing data, and 1000 bootstrap replicates . ABC transporter subfamilies were assigned to D. v. virgifera sequences and clades within this phylogenetic analysis by comparison to similarities from our BLASTx search results and tree topologies among nearest orthologous gene family members in T. castaneum [30, 32], and D. melanogaster. Multiple sequence alignments were generated as described above, wherein the deduced D. v. virgifera amino-acid sequences included full-length sequences when possible, but some were incomplete partial-protein sequences. All phylogenetic reconstruction methods were performed as described above.
Gene expression across developmental stages
Preliminary analysis to estimate the relative expression levels for eight transcripts (DvvABCA_50718, DvvABCB_39715, DvvABCE_2830, DvvABCF_2701, DvvABCG_3712, DvvABCG_14042, Dvvw and DvvABCH_5118) across growth stages was made via semi-quantitative PCR in order to ensure dsRNA injections would be performed prior to the time of corresponding peak expression. Total RNA was extracted from each developmental stage [embryo (E), larval (L), pupal (P), and adult male (M) and female (F)], from which cDNA was reverse transcribed using the Superscript™ III First-Strand Synthesis System (Invitrogen, Carlsbad, CA, USA) using an anchored poly(T) primer. These cDNA pools were then used individually as template in eight separate PCR reactions each using D. v. virgifera ABC transporter transcript-specific primer pairs (Additional file 9: Table S3). Primers for the D. v. virgifera ribosomal protein S6, DvvRPS6, were used as an external control. PCR reactions were set up using MyTaq™ DNA polymerase according to manufacturer instructions (Bioline, Memphis, TN, USA), and subsequent amplification reactions were performed in a C1000 Thermal Cycler (Bio-Rad Laboratories Inc., Hercules, CA, USA) with the following cycling conditions: (95 °C for 3 min), 25× (95 °C for 30s, 58 °C for 30s, 72 °C for 10s), (4 min incubation at 72 °C). Amplification products were then visualized and compared using 1.5% agarose gel electrophoresis.
RNAi knockdown phenotypes
Primers were designed for the generation of dsRNA using Vector NTI Advance (VNTI) software (Invitrogen), for all ABC genes whose orthologs are known to produce obvious RNAi phenotypes in T. castaneum . These primer sets targeted regions that encoded transcript-specific TMD domains; this was done in order to potentially reduce unintended off-target effects by avoiding the more conserved NBD domains. Partial cDNAs were amplified for the 8 genes (DvvABCA_50718, DvvABCB_39715, DvvABCE_2830, DvvABCF_2701, DvvABCG_3712, DvvABCG_14042, Dvvw and DvvABCH_5118), as described above for developmental stage expression. Nested PCR was performed with an initial denaturation of 95 °C for 3 min, 35 cycles at 95 °C for 30s, 58 °C for 30s, and 72 °C for 10s, and then a 4 min incubation at 72 °C on a C1000 Thermal Cycler (Bio-Rad). PCR products were purified using the QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s instructions, ligated into the pGEM-T vector (Promega, Madison, WI, USA), and the resulting plasmids were used to transform TOP10 competent E. coli (Invitrogen). All positive clones were cultured in a selective LB medium containing 100 mg ampicillin L− 1. The recombinant plasmid DNAs were isolated using the QIAprep® Spin Miniprep Kit (Qiagen), and the inserts were Sanger sequenced and confirmed by use as BLASTn queries (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Purified plasmids with each cloned ABC transporter were used as template in separate PCR reactions primed with the following primers: T7 as a forward primer (due to location in pGEM), and a pGEM-specific reverse primer that was tailed with T7. This enabled all amplification reactions to be performed using the same set of primers under conditions described above. PCR products were analyzed by 1.5% agarose gel electrophoresis, purified using the QIAquick PCR Purification Kit (Qiagen), and then ~ 1 μg of each was used as template for dsRNA synthesis using the MEGAscript T7 in vitro Transcription Kit (Ambion, Austin, TX, USA). Each of the synthesized dsRNAs were purified using the MEGAclear Kit (Ambion) and concentration determined using a Nanodrop 1000 (Thermo Scientific, Waltham, MA, USA) using the single-stranded RNA setting.
RNAi assays were conducted by injecting dsRNA corresponding to each of the 8 specific D. v. virgifera ABC genes individually into the hemocoel of third-instar larvae, pre-pupae and/or newly-eclosed female adults. Before microinjection, experimental insects were anesthetized on ice for 30 min, then injected with ~ 0.2 μl of a gene-specific dsRNA at a concentration of 1-2 μg/μl. Each treatment was replicated three times, with ≥20 individuals in each replicate. Following injection, larvae and pre-pupae were allowed to recover at room temperature for 1 hour, and then moved to germinated corn for further monitoring and phenotypic analysis. Phenotypes were observed daily using a stereomicroscope, and transcript levels assessed at 5 days post-injection by semi-quantitative PCR using RNA isolated from pools of injected individuals (one individual per replicate, for a total of three individuals per PCR reaction).
Treated females were kept in an oviposition chamber (agar plate with cheese cloth) and maintained on an artificial diet. At 2 days post-injection, females were mated to untreated males, and generally started to lay eggs ~ 10 days later. To determine egg viability, eggs were harvested from the oviposition chamber and placed on moistened filter paper in Petri dishes and held at 26 °C, 70% relative humidity with an L14:D10 photoperiod. Females were allowed to lay eggs over a two-week period, and eggs were counted every other day to assess the rate of egg laying. Hatch rate counts were made every other day, beginning 10 days after the first egg lay (22-days post-injection) and continuing for 4 weeks until no further hatching was observed.
We thank Pei-Shan Wu, Sofia Pinzi, Teresa O’Leary, Lauren Slayton and Wanose Getachew for their expert assistance in rearing WCR. This article reports the results of research only and any mention of products or services does not constitute an endorsement by USDA-ARS. USDA-ARS is an equal opportunity provider and employer.
FA and MDL conceived and designed the experiments; FA performed the experiments; FA, NG, BC, BW, MDL analyzed the data; and FA, NG, BC, MDL wrote the manuscript. All authors have read and approved the manuscript.
This work was supported by a grant from the Monsanto Corn Rootworm Knowledge Research Program, grant number AG/1005 (to ML), and start-up funds to ML from NC State University. Portions of this work by BC was supported by a joint United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (CRIS Project 5030–22000-018-00D), ARS SCINet computational resources (https://www.ars.usda.gov/scinet/), and the Iowa Agriculture and Home Economics Experiment Station, Ames, IA (Project 3543). USDA-ARS is an equal opportunity employer and provider. The funding bodies had no role in the design of the study, collection, analysis, or interpretation of data, or in writing the manuscript.
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The authors declare that they have no competing interests.
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