Transcriptome analysis reveals a positive effect of brassinosteroids on the photosynthetic capacity of wucai under low temperature
Brassinosteroids (BRs) have a positive effect on many processes during plant growth and development, and in response to various abiotic stressors. Low-temperature (LT) stress constricts the geographic distribution, growth, and development of wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen). However, there is little information on the global gene expression of BRs under LT stress in wucai. In this study, the molecular roles of 24-epibrassinolide (EBR) after exogenously application, were explored by RNA sequencing under LT conditions.
According to the Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, photosynthesis was significantly enriched after spraying EBR under LT. The transcripts encoding the photosystem II (PSII) oxygen-evolving enhancer protein, photosystem I (PSI) subunit, light-harvesting chlorophyll protein complexes I and II, and ferredoxin were up-regulated after the application of EBR. Transcripts encoding several key enzymes involved in chlorophyll biosynthesis were also up-regulated, accompanied by significant differences in the contents of 5-aminolevulinic acid (ALA), porphobilinogen (PBG), protoporphyrin IX (Proto IX), Mg-protoporphyrin IX (Mg-proto IX), protochlorophyllide (Pchl), and photosynthetic pigments. Notably, transcriptional and physiological analyses revealed that under LT stress, plant responses to EBR involved a major reorientation of photosynthesis, as well as porphyrin and chlorophyll metabolism.
This study explored the role of EBR as an LT stress tolerance mechanism in wucai. At the transcription level, LT tolerance manifests as an enhancement of photosynthesis, and the amelioration of porphyrin and chlorophyll metabolism.
KeywordsRNA-Seq Low-temperature stress Photosynthesis Porphyrin and chlorophyll metabolism
- B. rapa
- Chl a
- Chl b
Control with 24-epibrassinolide
Different expression gene
Kyoto Encyclopedia of Genes and Genomes
- LT + EBR
Low temperature with 24-epibrassinolide
- Mg-proto IX
Membrane stability index
- Proto IX
Real time quantitative PCR
The reads per kilo bases per million reads
Specific leaf area
- Total Chl
Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a variant of non-heading Chinese cabbage (Brassica campestris L.), a crucial species in the Brassicaceae family . As an important autumn and winter vegetable crop, wucai is cultured throughout most parts of China, especially in the Yangtze-Huaihe River Basin. With a beautiful shape and high levels of 70 vitamins and minerals, wucai is becoming an increasingly popular crop in other countries.
Brassinosteroids (BRs) were isolated from Brassica pollen ~ 40 years ago, and they are a type of plant steroid hormone. 24-epibrassinolide (EBR), one of the BRs, is biologically-active brassinolides hormones used in physiological and molecular studies . Moreover, previous studies found that BRs regulate a variety of physiological and developmental processes, including plant stress tolerance, leaf elongation, and flower development, which up-regulate large genes involved in cell division and differentiation, and exhibit control over all developmental processes . BRs are known to regulate photosynthesis under normal as well as in abnormal conditions [4, 5]. The studies of Choudhary et al.  and Yuan et al.  showed that foliar application of EBR can increase plant chlorophyll (Chl) content and up-regulate the expression levels of various oxidative stress marker genes, thereby improving photosynthetic capacity to enhance the tolerance of Arabidopsis and Cucumis sativus L. responding to different stresses.
Low-temperature (LT) stress is harmful to numerous plant physiological processes and is the main abiotic stressor that limits plant growth and development. For example, LT stress can stunt plant growth, disrupt photosynthesis, and reduce chlorophyll content, resulting in significant yield and economic losses. Recently, our team reported that the differentially expressed proteins in wucai (Brassica campestris L.) produced by temperature stress were mainly enriched in porphyrin and chlorophyll metabolism, carbon metabolism, carbon fixation in photosynthetic organisms, and so on . Previous research demonstrated that the exogenous application of BRs significantly improved resistance to LT stress by regulating the morphological, physiological, and biochemical characteristics of pepper (Capsicum annuum L.) [9, 10], tomato (Solanum lycopersicum Mill.) , and cucumber (Cucumis sativus L.) . Transcriptome analysis indicated that the DEGs of Winter Rapeseed (Brassica rapa L.) under cold stress were significantly enriched in plant hormone signal transduction, starch and sucrose metabolism, and photosynthesis . Jaglo illustrated that the freezing tolerance of B. napus can be enhanced through CBF-mediated “regulon engineering” . The results of these studies indicate that exogenous applications of BRs reduce the adverse effects of LT stress by increasing chlorophyll content, maintaining photosynthesis, and activating gene expression and signal transduction pathways [15, 16].
Physiological parameter effects of EBR
The largest change was in the total soluble sugar content among the 4 indicators. Compared to Cont, the total soluble sugar content increased by 56.87% in LT plants (Fig. 2c). Furthermore, the total soluble sugar content of LT plants significantly decreased after spraying EBR under LT stress (37.59%, Fig. 2c). The MDA content followed a similar trend. Compared to Cont, MDA content significantly increased by 49.81% in LT plants, and significantly decreased by 30.95% in LT + EBR (Fig. 2d). For the LT treatments specifically, SLA was depressed by 36.33%. Compared to LT, SLA significantly increased by 46.80% in LT + EBR plants (Fig. 2a). Compared to Cont, the MSI content of LT was significantly reduced by 2.07%. In contrast, MSI significantly increased by 1.14% after EBR treatment (Fig. 2b). Compared to LT, SLA significantly increased by 3.27% in LT + EBR (Fig. 2a).
Mapping and quantitative assessment of Illumina sequences
Two libraries were constructed from the LT and LT + EBR treatment groups for analysis by RNA-Seq. A total of 53.51 million (LT) and 50.83 million (LT + EBR) reads were generated. After removing the adapter, poly-N, low-quality, and empty reads, > 6 billion clean bases were obtained with a Q30 percentage > 94%, and a GC percentage between 47.4–47.7% (Additional file 5: Table S1). The quality control (fastqc v0.11.5, Illumina) and reads mapping (fastqc v0.11.5, Illumina) were shown in Additional file 1: Figure S1 and Additional file 2: Figure S2.
Each library of clean reads was aligned to the Brassica rapa (B. rapa) reference genome . The proportion of clean reads in the 2 wucai transcriptome libraries that mapped to the B. rapa reference genome ranged from 86.67–87.56% (Additional file 6: Table S2). A total of 30,843 genes were confirmed from the mapped libraries. All of the RNA- sequence data in this article have been deposited in the NCBI- SRA database and are accessible in SRP200451 (https://www.ncbi.nlm.nih.gov/sra/SRP200451).
GO and KEGG analyses of the EBR-induced DEGs
Among the DEGs, there were 7 pathways with Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations that were significantly affected: (1) photosynthesis–antenna proteins, (2) photosynthesis, (3) plant hormone signal transduction, (4) flavonoid biosynthesis, (5) pentose and glucuronate interconversions, (6) quorum sensing, and (7) phenylpropanoid biosynthesis (p < 0.001; Fig. 4b). Higher enrichment scores and more genes were observed in photosynthesis–antenna proteins (24 genes) and photosynthesis (24 genes).
Quantitative real-time PCR (qRT-PCR) analysis
Effects of EBR on Porphyrin and chlorophyll metabolism analysis
Effect of EBR on photosynthesis
BRs are a series of naturally present plant steroids associated with several critical physiological and cellular plant processes when experiencing a variety of environmental stressors . BRs are a category of steroidal growth regulators that promote stress tolerance and crop yield [22, 23]. Although previous studies on BRs have demonstrated that the functions of exogenous BRs can mitigate the damage induced by abiotic stress [24, 25], the associated regulatory mechanisms, especially the underlying molecular mechanisms, have received little attention. In this study, through physiological and biochemical analyses, it was demonstrated that an EBR pretreatment improved the cold tolerance of wucai. Then, in order to focus on the EBR-mediated variety in gene expression, RNA-Seq was used to investigate the part of EBR that responded to LT at the global transcriptome level. To the best of our knowledge, this study is the first demonstration of the molecular mechanisms of wucai responses to EBR treatments under LT. In order to determine the effect of EBR on LT tolerance, the discussion is focused on contrasting the LT and LT + EBR treatment groups. Based on the GO and KEGG analyses, DEGs correlated with the application of exogenous EBR under LT were notably enriched in genes associated with photosynthesis and chlorophyll biosynthesis.
Physiological parameters verify the role of exogenous EBR
Variations in leaf structure patterns represent the adaptation of many species . Higher SLA helps with higher potential evaporation requirements and wider foliar display, capturing more light for use in constant biomass investment . MDA content is a main indicator for evaluating the degree of membrane oxidation. Low MDA content leads to a higher tolerance of LT stress. Soluble sugars play a prominent, central role in plant structure and metabolism at the cellular and whole organism levels . MSI can be used to assess the integrity of the plasma membrane ; the higher the MSI value, the more complete the plasma membrane. In this study, the results of SLA, MSI, total soluble sugar content, and MDA indicate that EBR can significantly alleviate injury to LT. In order to explore the underlying molecular mechanisms, transcriptome sequencing was performed on LT and LT + EBR plants.
Exogenous EBR improves chlorophyll biosynthesis by promoting the conversion of intermediates
Chlorophyll is an important component in photosynthesis, as it plays a role in the transmission, distribution, and transformation of light energy . Chlorophyll content is closely related to photosynthetic rate and organic matter accumulation . Some studies have shown that exogenous application of EBR accelerates the increase of chlorophyll content in plants under stress [31, 32, 33]. Chlorophyll metabolism is an intricate pathway that involves the biosynthesis and degradation of chlorophyll. Chlorophyll biosynthesis is catalyzed by a diverse set of enzymes and can be restrained by abating the activity of all of these enzymes . The results of this study indicate that exogenous EBR treatment can increase the content of photosynthetic pigments under LT stress and promote a spectrum of enzymatic reactions in chlorophyll biosynthesis.
ALA is the first precursor of chlorophyll biosynthesis. Therefore, ALA biosynthesis has a considerable effect on the rate of chlorophyll biosynthesis . In this study, a significant increase in endogenous ALA was detected in LT + EBR plants compared to LT. Additionally, the conversion of ALA to chlorophyll includes many intermediates such as PBG, Proto IX, Mg-Proto IX, and Pchl. EBR application caused significant increases in the ALA and PBG contents, and significant decreases in Proto IX, Mg-Proto IX, and Pchl compared to LT. Four transcripts encoding 4 of the key enzymes involved in catalyzing the synthesis of these products were all up-regulated by exogenous EBR application. CHLM encodes a magnesium-protoporphyrin O-methyltransferase (EC:188.8.131.52), which catalyzes the transformation of Mg-protoporphyrin IX to Mg-protoporphyrin IX 13-monomethyl ester, accompanied by a significant decrease in Mg-Proto IX. POR encodes a protochlorophyllide reductase (EC:184.108.40.206), which catalyzes the transformation of protochlorophyllide to chlorophyllide a, accompanied by a significant decrease in Pchl and a significant increase in photosynthetic pigments. These results suggest that applications of exogenous EBR can modulate the synthesis of endogenous ALA and its transformation to intermediate products, thereby improving chlorophyll biosynthesis.
Exogenous EBR weakened stress-induced photoinhibition and enhanced photosynthesis
Photosynthesis is among the primary plant processes that are affected most frequently by abiotic stress [35, 36]. PSII is believed to play an important role in plant responses to environmental stress . For example, chilling stress has been shown to inhibit leaf photosynthesis through PSII-related photoinhibition [38, 39]. Chlorophyll fluorescence transients quantitatively explain dynamic changes in the OJIP curve, which in turn quantitatively explains the PSII light energy absorption, conversion, electron transport, PSII action center, activity on the receptor and donor sides, and redox state of the electron transporter. In this study, GO and KEGG enrichment analyses suggest that EBR-mediated induction of photosynthesis, chloroplast thylakoid membrane, and chlorophyll biosynthesis may be the primary factors contributing to the distinct photosynthetic characteristics of the EBR transcriptome under LT conditions.
In contrast, the PSII activity level, which is reflected by ϕPo, was not affected in plants sprayed with EBR under normal temperature conditions (Fig. 7). ϕPo decreased in both LT and LT + EBR plants, especially in LT. This suggests that exogenous EBR can protect PSII under LT stress. Changes in F0 depend on the factors present during energy dissipation and PSII inactivation . PSII damage or inactivation can result in an increase in F0 . In this study, F0 increased under LT. However, the increase was smaller in LT + EBR than in LT (Fig. 7), indicating there was less relative damage to PSII in LT + EBR. Thus, exogenous EBR could alleviate damage caused by LT to photosynthetic reaction centers and reduce chilling photoinhibition.
The health of PSII is often used to determine the photochemical efficiency of the light reactions in photosynthesis II . Compared to LT plants, the exogenous application of EBR in wucai promoted the maintenance of photochemical health under LT stress due to higher ΦPSII activity. From the KEGG pathway analysis, EBR application significantly regulated a series of proteins involved in PSII, including the oxygen-evolving enhancer, 10 kDa and Psb27 proteins. The direct effect of EBR on these PSII proteins revealed that EBR could bind to PSII membranes in order to maintain their integrity and improve photosynthetic function despite osmotic stress .
This study provides insight into the function of exogenous EBR application on gene expression and predicted functions in wucai responding to LT stress. Compared to LT, SLA and MSI were significantly improved in LT + EBR. Additionally, the content of MDA and total soluble sugar significantly decreased in LT + EBR, indicating that exogenous EBR could alleviate LT injury. The transcriptome analysis revealed that EBR primarily affected the transcripts associated with photosynthesis, porphyrin and chlorophyll metabolism under LT stress. Specifically, a significant increase in chlorophyll content and significant changes in the chlorophyll fluorescence parameters (i.e., Vj, Fv/F0, and PIabs) were detected when LT + EBR was compared to LT. The regulation of the transcriptome in combination with the physiology of chlorophyll biosynthesis and photosynthesis demonstrates the protective mechanism of EBR treatment in response to LT stress. The DEGs identified here could be further investigated as molecular markers, or used in future functional analyses investigating responses to LT stress and EBR application.
Plant material and stress treatment
Wucai seeds were provided by the Vegetable Genetics and Breeding Laboratory of the College of Horticulture, Anhui Agricultural University. Seeds were germinated from plugs before being transplanted into pots containing a mixture of stroma and vermiculite (3:1, v/v). Seedlings were grown in a growth chamber at 25/18 °C (day/night), 300 μmol m− 2 s− 1 photon flux density, and 70% relative humidity with a 12:12 h photoperiod.
In a pilot study, 6 levels of EBR concentrations (0, 0.05, 0.08, 0.1, 0.12, and 0.15 μM) were explored for treating wucai plants following a previously described protocol . Based on the results of the pilot study, 0.1 μM EBR was identified as the optimum EBR concentration, which was subsequently used as the processing condition in the main experiment (Additional file 4: Figure S4).
Seedlings of uniform size at the 5–6 leaf stage (45 dafter planting) were selected and randomly divided into 2 groups for pretreatment. One group (n = 50) was sprayed with a 0.1 μM EBR solution, while the other group (n = 50) was sprayed with the same volume of ddH2O. Two d after pretreatment spraying, the seedlings in each pretreatment group were further divided into 2 groups. One group (n = 25) continued to grow in the suitable environment without LT stress, while the other group (n = 25) was grown under LT. In total, there were 4 treatment groups: (1) Cont plants grown in an ideal environment with the ddH2O pretreatment; (2) Cont+EBR plants grown in an ideal environment with the 0.1 μM EBR pretreatment; (3) LT plants grown with the ddH2O pretreatment; and (4) LT + EBR plants grown with the 0.1 μM EBR pretreatment. Seedlings in both the LT and LT + EBR groups were transferred to a phytotron set at 7 /3 °C (day/night), 300 μmol m− 2 s− 1 photon flux density, and 70% relative humidity with a 12:12 h photoperiod. Each treatment was replicated 3 times in the phytotron, and each treatment consisted of 25 plants. After 5 d of LT, functional leaf samples from 3 biological replicates were collected from LT and LT + EBR for sequencing. All of the samples were collected at the same time, ground into powder in liquid nitrogen, and immediately stored at − 80 °C for future analyses.
Measurement the contents of SLA, MSI, Total soluble sugar and MDA
SLA was determined according to Choong et al.  with minor modifications. SLA was calculated by dividing the area of 10 diameter leaf discs (0.6 mm). Then, the leaf samples were placed in a constant temperature drying oven set at 105 °C for 15 min, and subsequently dried at 75 °C to a constant dry weight. SLA was calculated as the ratio of the leaf area to dry leaf weight.
After grinding, dry leaf samples were mixed with 4 mL 80% alcohol and shaken at 80 °C for 30 min. Cooled samples were centrifuged at 6855 r/min for 3 min, and the supernatant was collected. The residue was washed with 80% alcohol 3 times, and the supernatant was extracted after each wash. Then, all of the supernatants were brought to a set volume of 10 mL with 80% alcohol and decolorized with 0.01 g activated carbon at 80 °C for 30 min. After the addition of activated carbon, the material was filtered to obtain a filtrate, which was mixed with 6 mL anthrone and heated in a boiling water bath for 10 min. Samples were then ice-cooled and kept at room temperature (25 °C) for 10 min. After the chromogenic reaction, the optical density (OD) was spectrophotometrically recorded at 620 nm.
MDA is a product of lipid peroxidation, associated with plant oxidative stress. The MDA level of all of the plant samples was assessed through lipid peroxidation following the methods previously described by Mohammadi et al.  with minor modifications. Fresh leaf samples (0.5 g) were homogenized in 10 mL trichloroacetic acid (10%) and centrifuged at 4000 r/min for 10 min. Then, 2.0 mL supernatant was mixed with 2 mL thiobarbituric acid (0.6%), heated at 100 °C for 15 min, and cooled to room temperature. The absorbance of each aliquot was measured at 450 (A450), 532 (A532), and 600 nm (A600). The MDA content was calculated using the following formula:
RNA extraction, library construction and RNA sequencing
Total RNA was extracted from the LT and LT + EBR leaf samples using a mirVana miRNA Isolation Kit (Ambion, TX, USA) following the manufacturer’s instructions. RNA integrity was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). Samples with an RNA Integrity Number (RIN) ≥ 7 were subjected to subsequent analysis. Libraries were constructed using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, CA, USA) following the manufacturer’s instructions. Then these libraries were sequenced on the HiSeqTM 2500 or HiSeq X Ten Illumina sequencing platform (Illumina, CA, USA), which generated 125 bp/150 bp paired-end reads.
Sequence assembly and annotation
Each library generated > 6 gigabytes of raw data. Clean reads were filtered from the raw sequencing data and low-quality reads containing unknown nucleotides or adaptor sequences were removed; this procedure was performed following the methods previously described by Bolger et al. . Clean, filtered reads were aligned to the B. rapa reference genome by hisat2 .
Differential expression analysis
A differential expression analysis was performed for both LT and LT + EBR treatments based on the DESeq R package , which allowed for statistical analysis using a negative binomial (NB) distribution model. To control the false discovery rate (FDR), the FDR value calculation method was used to calculate the p-value of each gene. Then, the FDR error control method was used to perform multi-hypothesis test corrections on the p-value, where an adjusted p-value < 0.05 and |log2(foldchange)| > 1 was accepted to represent DEGs.
GO enrichment analysis of the DEGs was performed according to the GOseq R package. GO terms with the number of DEGs > 2 were regarded as significantly enriched (p < 0.05). Statistical enrichment of the DEGs was carried out in KEGG pathways using KOBAS software .
Validation of DEGs by qRT-PCR
Total RNA was extracted using the RNeasy extraction tool then reverse-transcribed into cDNA for the next analysis. A total of 20 transcripts were selected to verify the RNA-Seq analysis. Actin (ACT) was used as a reference gene to normalize the data. The SYBR fluorescent reagent (TaKaRa, RR820A) and Bio-Rad CFX96™ Real-Time System (Bio-Rad, CA, USA) were used to conduct qRT-PCR following the manufacturer’s instructions. The gene expression data were analyzed using the 2−ΔΔCt relative quantitative methods  in Microsoft Excel.
Measurement of photosynthetic pigment content and chlorophyll biosynthesis intermediate contents
In order to estimate the concentration of ALA, 0.5 g leaf samples were homogenized in 5 mL trichloroacetic acid (4%) and the extract was centrifuged at 14,541 r/min for 15 min. Then, 3 mL supernatant was added to 1.7 mL sodium acetate and 0.1 mL of acetylacetone. The mixture was placed in a boiling water bath for 10 min then cooled to room temperature. Two mL supernatant was thoroughly mixed with an Ehrlich-Hg reagent (0.2 g HgCl2 + 1 g P-dimethyl amino benzaldehyde + 42 mL glacial acetic acid + 8 mL 70% perchloric acid). After 15 min in darkness, the absorbance of the resulting solution was determined spectrophotometrically at 553 nm .
PBG content was measured following the methods previously described by Bogorad et al. . Briefly, 0.5 g leaves were homogenized in 5 mL extraction buffer (0.6 M Tris and 0.1 M EDTA, pH was adjusted to 8.2 with HCl) and centrifuged for 10 min at 14,541 r/min. Two mL supernatant was thoroughly mixed with 2 mL Ehrlich-Hg reagent. After incubating in darkness for 15 min, the absorbance was measured at 553 nm.
The (Proto IX, Mg-Proto IX and Pchl contents were quantified following the methods previously described by Hodgins and Huystee et al. . Briefly, leaf tissues were extracted with 80% alkaline acetone and centrifuged at 11,873 r/min for 10 min at 4 °C. The absorbance of the supernatant was measured at 575, 590 and 628 nm. The contents of Proto IX, Mg-Proto IX and Pchl were calculated using the formula previously described by Hodgins et al. .
Measurement of chlorophyll a fluorescence transients
In vivo chlorophyll a fluorescence emissions were measured in 30 min dark-adapted leaves with a continuous excitation fluorometer Pocket Plant Efficiency Analyzer (PEA, Hansatech, UK). Data were sampled at 10 μs intervals for the first 300 μs, providing excellent time resolution of F0 and the initial rise kinetics. The time resolution of digitization was switched to slower acquisition rates as the kinetics of the fluorescence signal slowed. Chlorophyll a fluorescence transient was analyzed using the JIP-test formulae . The fluorescence intensity at 20 μs was considered to be F0, while the maximal fluorescence intensity was presumed to be equal to Fm, as the intensity was high enough to ensure the closure of all of the reaction centers (RCs) of PSII. Moreover, the fluorescence intensity at 300 μs (F300 μs), 2 ms (J-step, FJ), and 30 ms (I-step, FI) were also measured . The following parameters refer to time 0 (the start of fluorescence induction): (a) the specific energy fluxes for absorption per reaction center (ABS/RC), trapping (TRo/RC), electron transport (ETo/RC), and dissipation at the level of the antenna chlorophylls (DIo/RC); and (b) the normalized total complementary area above the OJIP transient or total electron carriers per RC (Sm = EC0/RC = Area/(Fm-F0)). Performance index (PI) on absorption basis was calculated as: PI (abs) = (RC/ABS) • [φPo / (1– φPo)] [ψo / (1– ψo)]. Maximum quantum yield of primary photochemistry was calculated as: φPo = Fv / Fm = (Fm - Fo) / Fm . Other parameters measured in this study are provided (Additional file 12: Table S8).
All of the data from the 4 treatments were subjected to an analysis of variance (ANOVA). The mean separation was performed using the Fisher’s protected least significant difference (LSD) test with a significance level of p < 0.05. Analyses were conducted using SPSS v19.0 for Windows (SPSS Inc., USA). Figures were plotted using GraphPad Prism v7.0 and Origin Pro v9.1 software (OriginLab Corporation, MA, USA).
CW is the project leader. MZ performed research and wrote the manuscript; MZ and LY designed the experiments. MZ and JW carried out the experiments. LY and SX helped to modify the manuscript. YZ, LN, GC, JH and SZ supervised the study. All authors have read and approved the final manuscript.
This work was supported by the National Key R & D Program of China (2017YFD0101803) for the study and collection of data, the Major Science and Technology Projects of Anhui Province, China (17030701013) for analysis of data, the National Natural Science Foundation of China (No. 31701910) and the Natural Science Foundation of Anhui Province, China (1908085MC96) for interpretation of data.
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
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